생쥐에서 황사로 인한 항원관련 천식성 기도 염증과 기도 과민성에 미치는 영향

임흥빈(Heung Bin Lim),김승형(Seung Hyung Kim) 환경독성보건학회 2010 환경독성보건학회지 Vol.25 No.2

This study was carried out to investigate whether asian yellow sand dust (AS) has promoting effects of allergen-related airway inflammation and airway hyperresponsiveness, because the number of patient with allergic asthma and atopy, and with chronic bronchial inflammation and pneumonia have increased steadily in the cities of Korea. The appearance of AS collected was all round and nat, and the diameter was mostly below about 5 ㎛. When mice were treated with AS suspension by intratracheal instillation combined with ovalalbumin (OVA) sensitization chronically, the level of serum L-lactate dehydrogenase (LDH), IgE and histamine, and respiratory resistance was increased. Intratracheal instillation of AS and OVA also enhanced infiltration of eosinophils in the bronchoalveolar lavage fluid (BALF), IgE and eotaxin expression, and T helper type 2 cell derived cyrokines of interleukin (IL)-4, IL-13 and IL-5 as major contributors to allergy and asthma. These results indicate that AS elevates allergen-related airway inflammation and airway hyperresponsiveness in mice and may play an important role in the aggravation of respiratory diseases in Korea.

커피원두의 분쇄입도에 따른 커피 추출물의 이화학적 품질특성 및 휘발성 향기성분 분석

임흥빈(Heung-Bin Lim),장금일(Keum-Il Jang),김동호(Dong-Ho Kim) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.6

본 연구에서는 커피 분쇄입도 조건에 따라 변화되는 커피의 이화학적 품질특성 및 휘발성 향기성분 변화에 미치는 영향을 조사하기 위하여 분쇄된 원두는 표준체(pore size 850, 600 및 425 μm)를 사용하여 입자 크기를 whole bean(Control), 850 μm 이상(coarse), 850~600 μm(medium), 600~425 μm(fine), 425 μm 이하(very fine)의 크기가 되도록 사별하여 커피 추출 시료를 제조하였다. 품질특성으로는 pH, 산도, 갈색도, 색도, 총페놀, 카페인, 클로로겐산 함량 및 휘발성 화합물을 비교 분석하였다. pH 및 갈색도는 분쇄입도가 작아질수록 4.84~5.18, 0.257~0.284로 증가하였으나, 산도는 0.31~0.17로 감소하였다. 색도에서 L, a 값은 분쇄입도가 작아질수록 감소하였으나 L 값에서 분쇄입도별 유의적인 차이는 나타나지 않았으며 b 값은 분쇄입도가 제일 작은 very fine size(425 μm 이하)에서 15.75로 가장 높은 황색도를 나타내었다. 색차에서 Control과 분쇄 커피분말과의 차이는 11 이상의 색차값을 나타내어 현저한 색의 차이 단계를 나타내었다. 총페놀, 카페인 및 클로로겐산 함량은 분쇄입도가 제일 작은 very fine size 입도(425 μm이하)에서 추출한 커피가 4.54 mg GAE/mL, 733.0 및 383.7 μg/mL로 높은 함량을 나타내었고, 휘발성 화합물의 총함량도 100 mg/kg 이상 높게 검출되었다. 따라서 본 연구에서는 커피 추출의 기본 단계라고 할 수 있는 커피 분쇄입도 조건에 따라 변화되는 커피의 이화학적 품질특성을 상호 비교하고 향기성분 특성을 확인함으로써 커피 품질 평가에 관한 기초자료를 제시하였다고 생각된다. In this study, we investigated the physicochemical properties of coffee brews according to coffee bean grinding grade. We also examined the effect of grinding grade on amounts of volatile flavor compounds. Coffee brew samples were separated using standard sieves (with pore sizes of 850, 600, and 425 μm), making particle sizes of ground beans as follows: whole bean (control), 850 μm or more (coarse), 850∼600 μm (medium), 600∼425 μm (fine), and 425 μm or less (very fine). For each particle size category, pH, total acidity, brown color intensity, chromaticity, total phenolic content, caffeine content, chlorogenic acid content, and total amounts of volatile flavor compounds generated were compared and analyzed. As grinding grade decreased, pH and brown color intensity increased from 4.84 to 5.18 and from 0.257 to 0.284, respectively, whereas total acidity decreased from 0.31 to 0.17%. As grinding grade decreased, the L* and a* color values decreased; however, L* value did not exhibit a significant difference depending on the grinding grade. The b* value was 15.75 in the very fine size category, which showed the highest yellowness. There was an 11 or higher color difference between the control and ground coffee powder, indicating a remarkable color difference. The total phenolic, caffeine, and chlorogenic acid contents of the coffee brewed from ground beans with a very fine size were 4.54 mg gallic acid equivalent/mL, 733.0 μg/mL, and 383.7 μg/mL, respectively, which were high values. The total amounts of volatile compounds in the very fine size category were found to be greater than 100 mg/kg. In this study, we suggest the basis for coffee quality evaluation, which involves evaluating changes in the physicochemical properties and amounts of flavor compounds of coffee relative to the grinding grade of the beans (basic step of coffee extraction).

TRNSYS 건물 에너지 시뮬레이션 모델 캘리브레이션: GenOpt를 이용한 사례 연구

임흥빈(Lim, Heung-Bin),이경호(Lee, Kyoung-Ho),공민석(Kong, Min-Suk),서동현(Seo, Dong-Hyun) 한국건축친환경설비학회 2019 한국건축친환경설비학회 논문집 Vol.13 No.4

This paper demonstrates a calibration methodology for adjusting model parameters and factors using assistant tools - python and GenOpt. In this study, a building simulation model is developed for a demonstration building for a low-energy solar house. The model is composed of a 3D model describing building architecture and HVAC system model using TRNSYS simulation program. In this study, model calibration methodology consists of two phases in order to improve the accuracy of building energy model and reduce errors of simulation results: Parameter adjustment phase and factor optimization phase. Trial and error approach is used for the first phase and GenOpt tool is employed for the second phase to improve the model performance. Based on American Society of Heating, Refrigerating and Air-conditioning Engineers (ASHRAE) Guideline 14 indices, the performance of the calibrated building simulation model is evaluated to obtain Normalized Mean Bias Error (NMBE)hourly, values within ±10% and Coefficient Variation of Root Mean Square Error (CV (RMSE))hourly, values within 30%. The performance indices are calculated with simulated and measured temperatures of each indoor zone and buffer tank. Daily data is used for calibrating heat pump (HP) electrical energy consumption because it is hard to fit the operating time of HP which can be different to time that heating load occurs. As a result of the calibration process, it is found that the values of NMBEhourly and CV (RMSE)hourly achieves within followings: Average indoor temperature - NMBEhourly is 1.2% and CV (RMSE)hourly is 2.2%. Buffer tank temperature NMBEhourly is 1.5% and CV (RMSE)hourly is 8.2%. And the value of NMBEdaily and CV (RMSE)daily are used for HP electrical use as following: NMBEdaily is 9.5%, CV (RMSE) is 23.4%.

저에너지주택 CO2 기반 환기모델예측제어를 위한 실내오염도 학습모델

임흥빈(Heung Bin Lim),이경호(Kyoung Ho Lee),공민석(Min Suk Kong) 대한설비공학회 2018 대한설비공학회 학술발표대회논문집 Vol.2018 No.11

본 연구는 건물의 실내 CO₂를 이용하여 환기장치를 모델예측제어하기 위해 필요한 물리적 학습 모델을 구성함에 있어 모델 파라메터로써 유효실체적, 침기량, 누기량을 구하기 위해 침기량과 누기량의 개수를 달리하며 그 결과를 비교하였다. 파라메터와 사용 데이터의 단순화가 될수록 이를 보상하기 위해 모델 파라메터를 학습하며 실체적이 늘어난 결과가 나타나며 파라메터의 개수가 적을수록 모델의 학습 성능은 낮아지는 것으로 나타났다.

주거복지 및 연관 분야의 교육, 상담, 정보 제공 서비스 실태

임흥빈(Heung Bin Lim),문소희(So-Hee Moon),이현정(Hyun-Jeong Lee) 한국생활과학회 2016 한국생활과학회 학술대회 Vol.2016 No.11

담배연기 응축물중 phenolic fraction의 분리와 확인

이문수,임흥빈,장기철,Rhee, Mun-Su,Lim, Heung-Bin,Jang, Ki-Cheol 한국연초학회 1988 한국연초학회지 Vol.10 No.1

The phenolic compounds were isolated from the cigarette smoke condensate using two immiscible organic solvents by a partition coefficient. Of them, 20 phenolic compounds were identified by GC and GC/MS. The amount of the phenolic compounds was phenol, p-crestol, hydroxybensoic acid in order. And there was no difference in composition of these compound dependent on leaf tobacco . About 70% amount of these compounds were absorbed to cellulose acetate filter used. The contents and composition of these compounds were various in the commercial cigarette.

Burley 21 잎담배에서 건조기간중의 정유성분 조성의 변화

홍열,임흥빈,Hong, Yeol,Lim, Heung-Bin 한국연초학회 2003 한국연초학회지 Vol.25 No.1

The essential oils from Burley 21 tobacco was isolated by using the SDE (Simultaneous Distillation & Extraction) apparatus coupled with gas chromatography and mass spectrometry analysis. Burley 21 tobaccos were divided and collected by six stages at intervals of five days during air-curing process. Air curing was conducted with horizontal hanging method in greenhouse settled by shading materials. 55 components were identified by comparisons of retention indices and mass spectral data, including 22 hydrocarbons, 6 alcohols, 3 aldehydes, 9 ketones and 15 miscellaneous compounds. Neophytadiene was the major components of the oils and almost all hydrocarbons were gradually decreased during air-curing process. Most of alcohols were also diminished, on the contrary, 1-pentanol and benzyl alcohol among them were increased. Aldehydes and ketones were increased during air-curing and especially, the concentrations of solanone, $\beta$-damascone, $\beta$-damascenone and megastigmatrienones were much increased. Indole level of miscellaneous compounds were continuously increased during air-curing of Burley 21 tobacco.

황색종 NC82와 KFl14의 건조단계별 정유성분의 변화

홍열,임흥빈,석영선,신주식,김종열,나도영,이학수,Hong, Yeol,Lim, Heung-Bin,Seok, Young-Sun,Shin, Ju-Sik,Kim, Jong-Yeol,Ra, Do-Young,Lee, Hak-Su 한국연초학회 2001 한국연초학회지 Vol.23 No.2

Essential oil in tobacco leaves influences the taste and aroma of cigarette smoke and is important to tobacco quality. This study was conducted to investigate the change in the level of essential oil components during flue-curing process of two flue-cured tobaccos, NC82 and KEl14. Flue-curing process was divided by six steps; harvest stage, the end of yellowing stage, the middle of color fixing stage, the end of color fixing stage, the middle of midrib drying stage, full-cured stage. NC82 in each stage contained 0.28%, 0.30%, 0.35%, 0.36%, 0.40% and 0.42% essential oil, respectively, and KF114 were 0.29%, 0.31%, 0.34%, 0.36%, 0.39% and 0.41%, respectively. Almost all hydrocarbons on the basis of relative peak area were gradually increased in two varieties with curing, neophytadiene content in them was highest at the full-cured stage. Most of alcohols and esters with curing showed a declining trend, but benzyl alcohol was increased in two tobaccos. Ketones were largely increased at the midrib drying stage during the curing process, especially, the most largely increasing constituent was $\beta$-damascenone among them. The content of 2-butylterahydrofuran, heterocyclic compounds, was largely increased at tile color fixing stage. There was no considerable difference between NC82 and KFl14 at the GC profile of essential oil and the pattern of each components during flue-curing process.

흰쥐 간 마이크로좀의 Cytochrome P - 450 과 Testosterone 및 16α

손형옥,임흥빈,이영구,이동욱,박희윤 ( Hyung Ok Sohn,Heung Bin Lim,Young Gu Lee,Dong Wook Lee,Hee Yun Park ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.4

The binding affinity of the differentially induced cytochrome P-450 (P-450) in rat liver microsomes with testosterone and 16α-hydroxytestosterone (16α-OH-T) was compared. Interaction of testosterone with all three microsomel P-450 of normal, MC- or PB-treated rats, gave type I spectral changes and their spectral binding constants (Ks) to P-450s were 35, 33, and 25 μM at 25℃, respectively. PB-inducible P-450s among three of these showed the highest affinity to testosterone, and the ratio of low to high spin conversion of P-450 by this compound showed also a similar tendency as the results of binding affinity. 16α-OH-T, one of testosterone metabolites, binding to all three P-450 exhibited a reversed type I spectral changes and their Ks values to normal, MC- and PB-inducible P-450 were 41, 28, and 23 μM, respectively, but binding of it with PB-inducible P-450 showed another Soret band near 460 nm. These results indicate that 16α-OH-T also binds to P-450s with a high affinity like testosterone, and it may be further metabolized to more polar compounds by P-450.

GC/MS를 이용한 담배연기중 Melamine과 Cyanuric Acid 분석

곽호근,이수현,임흥빈,Kwak, Ho-Geun,Lee, Soo-Hyun,Lim, Heung-Bin 한국연초학회 2009 한국연초학회지 Vol.31 No.2

In this study, we investigated the existence of melamine and cyanuric acid in cigarette smoke because leaf tobacco contains lots of nitrogen compounds and they are capable of pyrolytic synthesis while smoking. The extraction and derivatization conditions of two compounds were performed according to the procedure of U.S. Food and Drug Administration(FDA). Ky3R4F as reference cigarette was smoked on ISO condition(Puff volumne : 35 ml, duration : 60 sec, Interval : 2 sec). Compared with the results Ky3R4F and standard solution, retention time of two compounds was not corresponded. As a result, we concluded that melamine and cyanuric acid were not existed in cigarette smoke.