당뇨의 한의학적 치료에 대한 최근 연구동향

정한성,유정석,송범용,Jung, Han-Sung,Yu, Jung-Suk,Song, Beom-Yong 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.4

Objective The purpose of this study was to analyze the directions of Korean Medicine treatment of diabetes mellitus. Methods We reviewed the 52 studies about diabetes mellitus which had been published from 2000 to 2007. We selected those studies from the search engine of the web site of five journals. Those were the Journal of Korean Oriental Medical Society, Korean Journal of Oriental Physiology & Pathology, the Journal of Korean Acupuncture & Moxibustion Society, Korean Journal of Oriental Internal Medicine and the Journal of Korean Pharmacopuncture Institute. Results 1. The types of diabetes mellitus model in studies were the model used Alloxan(2cases), the model used Streptozotocin(41cases), NOD mice(1case), ob/ob mice(1case), db/db mice(5cases) and rats fed highfat diet(2cases). 2. The types of method in studies were pharmacopuncture(8cases), herbal medcine(47case) and both pharmacopuncture and herbal medcine(3cases). the types of materials in studies were single herb(24cases), multiple herbs(32cases) and both single and multiple herbs(4cases). 3. The types of evaluation criteria in studies were glucose, pancreas, liver, kidney, serum lipid, oxidative stress, nervous system, vascular system and immunity. Conclusions There have been reported many studies of diabetes mellitus in Korean Medicine. It requires to study further types of diabetes mellitus, kinds of herbs and complications of diabetes mellitus for Korean Medicine treatment of diabetes mellitus was covered the ground.

뉴스 데이터를 활용한 한계기업 탐지에 관한 연구

정한성 ( Han-sung Jung ),임희석 ( Heuiseok Lim ) 한국정보처리학회 2022 한국정보처리학회 학술대회논문집 Vol.29 No.1

한계기업은 성장가능성이 있는 기업들에게 돌아가야 할 자금 및 지원정책을 기업의 연명수단으로 전략하게 될 가능성이 있어 비효율적 자원배분을 초래하게 되며 이는 궁극적으로는 경제성장의 제약을 유발하게 된다. 따라서 본 연구에서는 뉴스 데이터를 활용하여 이러한 한계기업을 초기에 탐지할 수 있는 방법을 제안하고자 한다. 연구결과, 뉴스 데이터를 활용하였을 경우, 그렇지 않은 경우보다 모든 지표가 우수한 것으로 나타나 실제적인 문제에서의 적용 타당성과 가능성을 보였다. 이를 통해 기업은 부실화된 정도를 사전에 예측하여 경영 전략 재수립을 위한 지표로 활용할 수 있을 것이며, 투자자는 리스크를 관리할 수 있는 수단으로 활용될 수 있다.

공동주택 지하공간의 결로판정 기법에 관한 연구

정한성(Han-Sung Jung),최성호(Sung-Ho Choi),차병주(Byoung-Ju Cha),이승복(Seung-Bok Leigh) 대한설비공학회 2000 대한설비공학회 학술발표대회논문집 Vol.2000 No.-

江華郡의 肺吸蟲症에 關한 疫學的 調査

林漢鍾(Han-Jong Rim),李駿商(Joon-Sang Lee),鄭漢成(Han-Sung Chung),玄逸(Il Hyun),鄭啓憲(Kye-Hun Jung) 대한기생충학열대의학회 1975 The Korean Journal of Parasitology Vol.13 No.2

Sweet Bee Venom과 Bee Venom이 심박변이도(HRV)에 미치는 영향

육태한,유정석,정한성,Yook, Tae-Han,Yu, Jung-Suk,Jung, Han-Sung 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.1

Objective : In this study, we investigated the effects of Sweet Bee Venom(SBV) and Bee Venom(BV) at a acupoint, HT7(Shinmun) on the Heart Rate Variability(HRV) in the healthy man. And we tried to observe how Sweet Bee Venom and Bee Venom affects on the balance of the autonomic nervous system. Methods : We investigated on 22 heathy volunteers consisted of 10 subjects in SBV group and 12 subjects in BV group. Study form was a randomized, placebo-controlled, double-blind clinical trial. 22 subjects of each group were injected SBV and BV at HT7(Shinmun). And we measured HRV by QECG-3:LXC3203 (LAXTHA Inc. Korea) on 7 times : before and after injection per 5minutes during 30minutes. Results : 1. After SBV injection, Mean-RR was significantly high from 0 to 10 minutes, Mean-HRV was significantly low from to 10 minutes, SDNN was significantly high after 25minutes, Complexity was significantly high from 5 to 10 minutes and RMSSD was significantly high from 5 to 10minutes. 2. Complexity of SBV Group significantly decreased from 20 to 25minutes, RMSSD of SBV Group significantly increased from 10 to 15minute and from $20{\sim}25$minutes, SDSD of SBV Group significantly increased from 10 to 15 minute and from $20{\sim}25$minutes compared with that of BV group. 3. After SBV injection, Ln(VLF) was significantly from 25 to 30minutes. Conclusions : The results suggest that SBV in heathy adult man tend to activate the autonomic nervous system compared to BV within normal range.

생쥐 배아 혀 상피의 발생과정에서 나타나는 사이토케라틴 1, 14와 PAX 9에 관한 면역조직화학적 연구

정혜인(Hye-In Jung),진명욱(Myoung-Uk Jin),조제열(Je-Yoel Cho),정한성(Han-Sung Jung),김재영(Jae-Young Kim) 대한체질인류학회 2008 대한체질인류학회지 Vol.21 No.4

피부의 상피에 대한 연구는 부속기관의 발생 그리고 보호장벽 형성과 같은 다양한 분야에서 연구되어 왔다. 혀 상피조직의 발생과 관련된 보호장벽의 형성 및 상피조직 분화에 대해서는 많은 연구가 필요한 실정이다. 본 연구에서는 혀의 보호장벽이 형성되고 상피의 분화가 일어나는 발생중인 혀를 이용하여 보호장벽 형성과정을 면밀하게 관찰하였고, 피부의 상피에서 나타나는 다양한 사이토케라틴의 발현양상을 발생중인 혀에서 조직면역화학방법으로 확인하였다. 혀 상피의 발생과정에서 PAX 9의 발현이 사이토케라틴 14와 겹쳐서 나타나는 것을 확인할 수 있었으며, 또한 Ki67을 이용한 상피세포의 증식양상과 비교해 볼 때, 유사하게 나타나는 것을 확인할 수 있었는데 이는 PAX 9, 사이토케라틴 14와 세포의 증식이 혀 보호장벽 형성에 관여하는 중요한 인자로 작용할 수 있을 것이라고 생각된다. 이러한 다양한 상피분화관련 인자들의 발현양상들을 비교해 볼 때, 혀 상피의 분화는 E15에서 본격적으로 시작되어 보호장벽의 형성 이전에 사이토케라틴 14와 PAX 9과 같은 인자들의 발현이 먼저 나타나게 되면서 상피의 분화가 진행되고, 이후 사이토케라턴 1과 같은 각질화 관련 인자가 보호장벽 형성에 기여한다고 생각된다. Epithelial differentiation and morphogenesis in skin and oral mucosa were elucidated using various experimental tools. However, tongue epithelial differentiation has not been examined properly yet. In this study, we identified the relationship between morphological changes and localizations of differentiation markers, such as cytokeratins and PAX 9 in mice embryonic tongue development. Protective barrier formation and localization pattern of cytokeratins in tongue epithelium were examined with toluidine blue staining and immunohistochemistry respectively. Localization patterns of PAX 9 and Cytokeratin 14 were coincided during tongue epithelium development. In addition, compared with Ki67 localizations , marker for cell proliferation, localization patterns of PAX 9 and Cytokeratin 14 would suggest that these factors would involve in tongue barrier formation through cell proliferation. Based on these results, tongue epithelial differentiation would begin at E14 with the specific localizations of PAX 9 and Cytokeratin 14 prior to protective barrier formation then Cytokeratin 1, keratinization marker, would involve in protective barrier and filiform papillae formations.

한국인 하악 유합부에서의 피질골-해면골의 밀도 및 형태

민천기,박현도,김창성,정한성,조규성,김희진,최성호,Min, Cheon-Ki,Park, Hyun-Do,Kim, Chang-sung,Jung, Han-Sung,Cho, Kyoo-Sung,Kim, Hee-Jin,Choi, Seong-Ho 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.3

In performing implant procedures in the anterior portion of the maxilla, many difficulties exist because of anatomical reasons, such as the proximity of the nasal floor, lateral extension of the incisive canal, and labial concavity. On the other hand, in the posterior region of the maxilla, there is often insufficient recipient bone between the maxillary sinus and alveolar ridge due to alveolar ridge resorption and pneumatization of the maxillary sinus. In order to perform implants in such regions, ridge augmentation procedures such as onlay bone graft, guided bone regeneration, and maxillary sinus grafting are performed. In studies of Caucasians, use of autograft from mandibular symphysis has been reported to be highly successful in maxillary sinus grafting. However, in a clinical study of Koreans, autograft of mandibular symphysis has been reported to have significantly low success rate. It has been hypothesized that this is because of insufficient cancellous bone due to thick cortical bone. In order to test this hypothesis, bone quality and morphology of Koreans can be compared with those of Caucasians. In this study, the bone density and morphology of the cortical bone and cancellous bone in the mandibular symphysis of 35 Korean cadavers were evaluated. The following results were obtained: 1. In terms of bone density, type I, type II, and type III consisted of 1.4%(3/213), 72.3%(154/213), and 26.3%(56/213) of the cross-sectioned specimens, respectively. In general, the bone density tended to change from type II to type III, as cross-sectioned specimens were evaluated from the midline to the canine. Type IV wasn't observed in this study. 2. The distance between the root apex and the lower border of the cancellous bone was 18.34mm-20.59mm. Considering that the bone has to be cut 5mm below the root apex during the procedure, autografts with about 15mm of vertical thickness can be obtained. 3. The thickness of cortical bone on the labial side increased from the root apex to the lower border of the mandible. The average values ranged from 1.43mm to 2.36mm. 4. The labio-lingual thickness of cancellous bone ranged from 3.43mm to 6.51mm. The thickness tended to increase from the apex to the lower border of the mandible and decrease around the lower border of cancellous bone. From the above results, the anatomic factors of the mandibular symphysis (bone density, thickness, quantity and length of the cortical bone and cancellous bone) didn't show any difference from Caucasians, and it cannot be viewed as the cause of failure in autografts in the maxillary sinus for implants.

An Immune-compromised Method for Tooth Transplantation Using Adult Bone Marrow Stromal Cells and Embryonic Tooth Germ

권혁제(Hyuk-Jae Kwon),박지희(Gi-Hee Park),김은정(Eun-Jung Kim),이민정(Min-Jung Lee),정한성(Han-Sung Jung),조성원(Sung-Won Cho) 대한체질인류학회 2011 해부·생물인류학 (Anat Biol Anthropol) Vol.24 No.4

소실된 치아를 대체하기 위해 가장 좋은 치료법은 동일개체의 치아 또는 치배를 이식하는 것이지만, 사람에서는 이러한 치아나 치배를 얻기는 매우 힘들다. 동종 또는 이종 간의 치아 이식은 치주인대에서 발생하는 면역거부반응에 의해 대부분 실패하였다. 이번 연구는 성체의 골수간질세포를 배아의 치배와 결합시키는 방법을 사용함으로써, 치아 주위조직의 세포를 골수간질세포로 대치할 수 있는 지에 대해서 알아보았다. 실험결과로서, 골수간질세포에서 유래한 세포들을 치주인대와 이틀뼈에서 관찰할 수 있었다. 이는 골수간질세포가 치주인대와 이틀뼈의 형성에 참여하였다는 것을 의미한다. 만약 이와 같은 치아를 골수간질세포를 제공한 개체에게 이식한다면, 면역거부반응이 덜 일어날 것이다. 이처럼 성체 골수간질세포와 배아 치배를 재결합시키는 방법은 치아주위조직의 일부를 골수간질세포로 바꿔주기 때문에, 이종치배를 이용하여 만든 치아를 사람의 턱뼈에 이식할 수 있도록 하는 방법 중 하나가 될 수 있을 것이다. Tooth transplantation using autogenic adult teeth or embryonic tooth germs is the one of best treatments for replacement of missing teeth, but there are limitations in the accessibility. Isogenic or xenogenic tooth transplantation has been failed because of the immune rejection response occurring in the periodontal ligament of transplanted tooth. In this study, by utilizing the recombination between mouse embryonic tooth germ and mouse adult bone marrow stromal cells, we tried to replace the periodontal tissues such as periodontal ligament and alveolar bone with adult bone marrow stromal cells. At four weeks after the transplantation of the recombinant into a kidney, adult bone marrow-derived cells cells were observed in the periodontal ligament and alveolar bone. This result indicates that adult bone marrow stromal cells can participate in the formation of periodontal tissues. If these tooth and periodontal tissues are transplanted into host who donates adult bone marrow stromal cells, adult bone marrow-derived cells will be regarded as host cells, and immune rejection response will not occur in these cells. Therefore, it is suggested that recombination between adult bone marrow stromal cells and embryonic tooth germ is a good candidate method using xenogenic tooth germ for replacement of missing teeth in human by replacing cells in periodontal tissues with human adult bone marrow stromal cells.

ENU 돌연변이법에 의한 핍모증과 무치증을 보이는 유전성 마우스의 발견 및 기초 연구

김양언(Yang-Eon Kim),조재우(Jae-Woo Cho),조규혁(Kyu-Hyuk Cho),정한성(Han-Sung Jung),한상섭(Sang-Seop Han),송창우(Chang-Woo Song) 한국실험동물학회 2002 Laboratory Animal Research Vol.18 No.4

Expression Patterns of Ki-67, Cyclin A, and Cyclin D1 during Tooth Development

Hyuk-Jae Kwon(권혁제),Kyung-Sik Yoon(윤경식),Han-Sung Jung(정한성) 대한체질인류학회 2013 해부·생물인류학 (Anat Biol Anthropol) Vol.26 No.1

치아발생 및 형태형성 과정에서 치아상피와 치아간엽을 구성하는 세포는 동적인 세포주기의 변화가 일어난다. 현재까지 세포증식은 치아발생에 중요한 현상으로 알려져 있지만, 치아발생 중에 일어나는 복잡한 분자적 기전과 연관해서 세포주기의 각 시기가 어떻게 관여하는지에 대해서는 충분한 연구가 이루어지지 않았다. 그러므로 본 연구는 치아발생 기전과 세포주기의 시기의 변화와의 관계를 밝히고자 하였다. 치아발생 과정에서 일어나는 형태변화를 확인하기 위해 싹시기, 모자시기, 종시기의 쥐 앞니 및 어금니 치배를 헤마톡실린-에오신으로 염색하여 조직학적으로 관찰하였다. 또한 세포주기 시기의 표지자인 Ki-67, 싸이클린 A, 싸이클린 D1의 발현양상을 관찰하기 위해 면역조직화학염색을 시행하였다. 싹시기, 모자시기, 종시기에서 증식하는 세포들은 Ki-67과 싸이클린 A를 발현하는 것을 확인하였다. 싸이클린 D1은 앞니의 상아질모세포 및 모자시기의 사기질결절에서 특이적인 발현을 보였으며, 이곳에서는 Ki-67이나 싸이클린 A가 발현되지 않는다는 것을 발견하였다. 본 연구는 치아발생 중 각 주요 시기에서 세포주기의 변화를 관찰하였으며, 이는 치아발생에 관여하는 기전에 대한 중요한 정보를 제공한다. 또한 본 연구의 결과는 지금까지의 앞니의 사기질모세포 및 사기질결절의 특성에 대한 지식을 이해하는 데에 중요한 자료가 될 것으로 사료된다. Dental epithelial and mesenchymal cells that form the teeth undergo dynamic changes in cell cycle during tooth development and morphogenesis. Although proliferation has been known as a key event during odontogenesis, the cell cycle phases and their relations with the complicated molecular mechanisms of tooth development are not fully understood yet. This study comparatively examined the expression patterns of Ki-67, cyclin A, and cyclin D1 during tooth development in the mouse incisor and molar in order to identify the cellcycle characteristics during odontogenesis. We found that Ki-67 and cyclin A were expressed in the proliferating cells in the dental epithelial and mesenchymal tissues at the bud, cap and bell stages. Cycln D1 showed distinct expression in the incisor odontoblast region and the enamel knot, in which Ki-67 nor cyclin A was expressed. Our results provide specific information on the cell cycle phases during tooth development that may provide clues to relate them with the complex odontogenic mechanisms. Furthermore, we suggest that our findings enlightened the previous studies on the incisor odontoblasts and the enamel knot during tooth development.