Inoculation Effect of Methylobacterium suomienseon Growth of Red Pepper under Different Levels of Organic and Chemical Fertilizers

Min-Kyoung Lee(이민경),Gil-Seung Lee(이길승),Woo-Jong Yim(임우종),In-Soo Hong(홍인수),Pitchai Palaniappan,Md. Ashaduzzaman Siddikee,Hari P. Deka Boruah,Munusamy Madhaiyan,Ki-Sup Ahn(안기섭),Tongmin Sa(사동민) 한국토양비료학회 2009 한국토양비료학회지 Vol.42 No.4

화학비료와 유기질비료를 시비 수준 및 Methylobacterium suomiense CBMB120의 접종이 고추 생육에 미치는 영향을 확인하기 위하여 유기질비료와 화학비료의 시비량을 권장시용수준의 100%, 75% 및 50%로 처리하고 Methylobacterium suomiense CBMB120을 접종 한 후 정식 후 19, 36, 및 166일에 작물의 높이를 측정하였으며 이식 후 166일에 건물량 측정하였다. 균주를 접종한 처리구의 식물체 높이는 정식 후 19, 36일에 각각 14.17%, 10.03% 증가하였다. 건물 중 역시 10.30%, 6.84% 증가하여 유의성 있는 차이를 나타내었다. 또한 균주 접종은 유기질 비료 100% 시용구는 36일 후 고도의 유의성(p<0.01)을 갖는 차이를 보였으며 화학 비료 100% 시용구는 19일 후 고도의 유의성(p<0.01)을 갖는 차이를 나타내었다. 화학비료 100% 시용구에 균주를 접종하지 않은 처리 구와 화학비료 75% 시용구에 균주를 접종한 처리구의 생육을 분석한 결과 유의성 있는 차이가 없는 것으로 나타났다. 이는 향후 다양한 처리량 및 처리방법의 연구를 통하여 Methylobacterium suomiense CBMB120 균주의 접종이 화학비료를 일정 부분 감비 할 수 있다는 가능성을 시사한다. Use of plant growth promoting symbiotic and non-symbiotic free-living beneficial bacteria as external source of nitrogen is a major research concern for sustainable crop production in the 21st century. In view of this, an experiment was conducted under controlled conditions to determine the effects of inoculation with Methylobacterium suomiense CBMB120, a plant growth promoting (PGP) root and shoot colonizer on red pepper, for the purpose of reducing external chemical nitrogen fertilization. Amendments with organic fertilizer and chemical fertilizer in the form of NPK were made at dosages of 50%, 75% and 100%, at 425 and 115 kg/ha-1 measurements. The soil type used was loam, with a pH of 5.13. The growth responses were measured as plant height at 19, 36 and 166 days after transplantation and final biomass production after 166 days. It was found that inoculation with M. suomiense CBMB120 promotes plant height increase during the active growth phase at 19 and 36 days by 14.17% and 10.03%, respectively. Thereafter, the bacteria inoculated plantlets showed canopy size increment. A highly significant inoculation effect on plant height at p<0.01 level was found for 100% level of organic matter and chemical amendment in red pepper plantlets after 36 days and 19 days from transplantation. Furthermore, there was a significantly higher (10.30% and 6.84%) dry biomass accumulation in M. suomiense CBMB120 inoculated plants compared to un-inoculated ones. A 25% reduction in the application of chemical nitrogen can be inferred with inoculation of M. suomiense CBMB120 at with comparable results to that of 100% chemical fertilization alone. Enumeration of total bacteria in rhizosphere soil confirms that the introduced bacteria can multiply along ther hizosphere soil. Large scale field study may lead to the development of M. suomiense CBMB120 as an efficient biofertilizer.

Effect of Inoculation of Methylobacterium oryzae on the Growth of Red Pepper at Different Organic Fertilizer Levels

Puneet Singh Chauhan,Gil-Seung Lee(이길승),Min-Kyoung Lee(이민경),Woo-Jong Yim(임우종),Gyeong-Ja Lee(이경자),Young-Sang Kim(김영상),Jong-Bae Chung(정종배),Tong-Min Sa(사동민) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.4

다양한 유기질비료 수준에서 Methylobacterium oryzae CBMB20의 처리가 고추의 생육촉진에 미치는 영향을 조사하였다. 유기질비료 권장시비수준의 70, 85, 100, 120% 시용구에서 시비량이 증가함에 따라 고추의 신장, 건물중 및 수량에서 유의성 있는 증가를 확인하였다. M. oryzae CBMB20의 접종 처리에서 고추의 생장이 증가하였으며, 고추의 수량 또한 각 유기질 비료 수준별로 10~35% 증가하였다. 수확기에 조사한 근권토양의 메탄올자화세균의 밀도는 M. oryzae CBMB20 처리구에서 2.51~2.63 log CFU g<SUP>-1</SUP> soil 이었으며, 미처리구에서는 1.0 log CFU g<SUP>-1</SUP> soil 이하로 나타났다. 따라서 접종한 M. oryzae CBMB20은 고추 작물 근권토양에 효과적으로 군집하였으며, 식물생장촉진 호르몬의 분비 등의 작용을 통한 작물생장촉진효과를 발휘한 것으로 판단된다. M. oryzae CBMB20을 접종하지 않은 경우에는 고추의 최대 생장과 수량이 유기질 비료 권장시비 수준 120%에서 나타났으나, M. oryzae CBMB20을 접종한 경우에는 최대 생장과 수량이 권장시비 수준에서 나타났다. 이러한 결과는 식물생장촉진미생물의 접종을 통하여 비료 사용량의 절감 가능성을 제시하는 것이며, 식물생장 촉진 미생물 제제로서 M. oryzae CBMB20의 활용이 가능하리라 사료된다. Plant growth promoting ability of Methylobacterium oryzae CBMB20 was evaluated under different levels of organic fertilizer application on red pepper plants in a pot experiment. Oil cake as an organic N fertilizer was applied at the rates of 70, 85, 100 and 120% of the conventional recommended level. Each treatment was further treated with or without M. oryzae CBMB20 inoculation. The recommended amount of compost for red pepper was added in all the treatments. Results revealed that plant height, dry biomass and fruit yield were enhanced in increasing order as the rate of fertilization increased. Overall plant growth was improved due to the inoculation of M. oryzae CBMB20 and red pepper fruit yield was also increased by 10-35% in the plants inoculated with M. oryzae CBMB20 at different rates of organic fert1izer application. Total methylotrophic bacterial population in rhizosphere soil measured at the time of harvest was significantly higher in M. oryzae CBMB20 inoculated treatments. The growth promoting effect of M. oryzae CBMB20 found in red pepper could be due to the effective colonization of the bacteria in the rhizosphere and its ability of enhancing nutrient availability and producing plant growth hormones. With the plant growth promoting effect of M. oryzae CBMB20, the rate of organic fertilizer application can be reduced without any significant decreases in biomass production and yield of red pepper.

Effect of Methylobacterium oryzae CBMB20 Inoculation and Methanol Spray on Growth of Red Pepper (Capsicum annuum L.) at Different Fertilizer levels

Puneet Singh Chauhan,Gil-Seung Lee(이길승),Min-Kyoung Lee(이민경),Woo-Jong Yim(임우종),Gyeong-Ja Lee(이경자),Young-Sang Kim(김영상),Jong-Bae Chung(정종배),Tong-Min Sa(사동민) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.4

지속가능한 친환경농업을 위해서는 비료와 함께 식물생장촉진 미생물 또는 생장조절물질을 적절히 혼합 사용하는 것이 바람직하다. 본 연구에서는 Methylobacterium oryzae CBMB20의 근권토양접종과 메탄올 엽면살포에 따른 고추의 생육촉진 효과를 유기질 비료의 시용 수준별로 조사하였다. M. oryzae CBMB20의 근권토양접종과 메탄올 엽면살포는 각기 고추생육을 증대시켰다. 또한 이들을 동시에 혼합 처리하였을 경우에는 고추의 생장촉진 효과가 더욱 현저하게 나타났다. M. oryzae CBMB20의 근권토양접종과 메탄올 엽면살포에 따른 고추 생장촉진 효과는 유기질 비료의 시용수준이 낮은 경우에 더욱 현저하게 나타났다. M. oryzae CBMB20와 메탄올을 혼합 처리한 경우 권장시비수준 100%와 300% 처리 사이에 고추생육과 수량에서 유의성 있는 차이가 없었다. 시비량이 지나치게 많을 경우 생장촉진제의 처리효과를 거둘 수 없으며, 적정한 시비수준에서 M. oryzae CBMB20와 메탄올을 혼합 처리함으로써 고추를 비롯한 작물의 생장과 수량을 유지하면서도 시비량을 크게 줄일 수 있을 것으로 판단된다. Fertilizers, plant growth promoting microbes and plant growth regulators should be combined together and used in order to achieve a maximal plant growth and yield in modern sustainable and ecological agricultural systems. In this study rhizosphere inoculation of Methylobacterium oryzae CBMB20 and foliar application of methanol were tested for their ability to promote the growth of red pepper plant at different levels of organic fertilizer. Rhizosphere inoculation of M. oryzae CBMB20 and foliar spray of methanol could promote red pepper plant growth and yield, and the growth promoting effect induced by the combined treatment of M. oryzae CBMB20 inoculation and foliar spray of methanol was more distinctive. This result suggests that a synergistic growth promoting effect of methanol spray and M. oryzae CBMB20 inoculation can be obtained in the combined treatment of the two growth promoting factors. The growth promoting effect was more significant in the lower fertilization rate, and the plant growth was not significantly different between 100 and 300% fertilizer treatments where both M. oryzae CBMB20 inoculation and foliar spray of methanol were included. This result indicates that, with the plant growth promoting effect of M. oryzae CBMB20 and methanol, fertilizer application rate can be profoundly reduced without any significant decreases in biomass accumulation and yield of crops.

무게측정방식에 따른 Lever-linked Roberval Mechanism의 설계특성

안지윤 ( Ji Yun An ),안중환 ( Jung Hwan Ahn ),이길승 ( Gil Seung Lee ),김화영 ( Hwa Young Kim ) 한국센서학회 2020 센서학회지 Vol.29 No.5

The deflection and null balance methods are used for precision force measurement in the precision industry. Since both methods are based on deformation, the performance of the load cell mechanism is important. In this study, the design variables were obtained via the free body diagram of a lever-linked Roberval mechanism (combined with a flexible hinge link and a Roberval mechanism), and the design characteristics were analyzed according to the weight method. Based on the design characteristics, the optimal design was conducted according to the weight method and FEM was used to verify its reliability.