부산지역에서 분리한 레지오넬라균에 대한 PFGE를 이용한 molecular typing

박은희,김미희,김정아,한난숙,이주현,민상기,박연경,진성현,정구영,빈재훈,Park Eun-Hee,Kim Mi-Hee,Kim Joung-A,Han Nan-Sook,Lee Ju Hyeoun,Min Sang Gi,Park Yon Koung,Jin Seong Hyun,Jeong Gu Young,Bin Jae Hun 한국생명과학회 2005 생명과학회지 Vol.15 No.2

2001년부터 2003년까지 부산지역의 냉각탑수에서 분리한 L. pneumophila (serogroup 1) 39균주에 대해 제한효소 SfiI 처리한 경우 PFGE 양상은 dice coefficient $<65\%$의 유사성을 가진 band를 Awl로 10개의 pulsotype으로 나누었고, 가장 많았던 유형은 E pulsotype으로 39주중 18주로 $46.2\%$를 나타내었으며, 그 외 A pulsotype $17.9\%$, C pulsotype $15.4\%$, F pulsotype $7.7\%$ 및 B, D, G, H, I, J pulsotype이 각각 $2.6\%$로 유전적 양상이 다양하였다. 제한효소 NotI 처리한 경우 PFGE 양상은 dice coefficient $<60\%$의 유사성을 가진 band를 $a\~h$로 8개의 pulsotype으로 나누었고, 가장 많았던 유형은 f pulsotype이 $38.5\%$였으며 그 외 d pulsotype $20.5\%$, e pulsotype $17.9\%$, a pulsotype $10.3\%$, h pulsotype $7.7\%$ 및 b, c, g pulsotype이 각각 $2.6\%$를 차지하였다. 본 연구를 통하여 부산지역에서 분리되는 레지오넬라균에 대한 유전자 유형분석 결과를 데이터베이스화하여, 레지오넬라증의 집단 발생 또는 산발적인 질병 발생이 있을 경우 분자학적 측면에서 사람과 환경에서 분리된 균으로부터 감염원과 감염경로를 규명하는 역학적인 도구로써 이용 가능할 것으로 사료되었다. In this study, we did the molecular typing of 39 environmental Legionella pneumophila serogroup 1 isolates collected from 2001-2003 in Busan using the pulsed-filed gel electrophoresis (PFGE). PFGE of SfiI fragments were divided into 10 pulsotypes $(A\~J)$, corresponding to $<65\%$ similarity and a subtype within each pulsotype was characterized by $>84\%$ similarity. The major cluster was pulsotype E $(46.2\%)$, which included 18 isolates and was divided into 4 subtypes $(E1\~E4)$. PFGE of NotI fragments were divided into 8 pulsotypes $(a\~h)$, corresponding to $<60\%$ similarity and a subtype within each pulsotype was characterized by $100\%$ similarity. The major cluster was pulsotype f $(38.5\%)$, which included 15 isolates. The ATCC type strain L. pneumophila serogroup 1 was identified as a different molecular pulsotype compare to the Busan isolates. It is possible that L. pneumophila serogroup 1 isolated in Busan with specific DNA pattern is comparable with those isolation in other cities in Korea.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

중학생의 자의식이 기술·가정 교과 '옷차림과 자기표현' 단원의 중요도와 외모관리행동에 미치는 영향

박은희,이상주,Park, Eun-Hee,Lee, Sang-Joo 한국의상디자인학회 2016 한국의상디자인학회지 Vol.18 No.2

The object of this study was to find out the influence of juvenile self-consciousness on the importance of unit 'clothing and self-express' in technology home economics curriculum and appearance management behavior. Questionnaires were administered to 244 middle school students living in Deagu metropolitan City. Frequency, factor analysis, reliability analysis, regression analysis, and ${\chi}^2-test$ were used for data analysis. Our findings are as follows. Self-consciousness had factors as privately and socially self-consciousness and social anxiety. The factors of the importance of unit 'Clothing and self-express' were clothing, self-express and excellence in traditional clothing. Appearance management behavior had factors as appearance management, plastic surgery interest, diet importance, health focus and eating habit. Self-consciousness was found to have significant effects on the factors of the importance of unit 'Clothing and self-express', and self-consciousness on factors of appearance management behavior. The differences by gender of middle students was examined in clothing and textile interest, clothing and textile unit interest and knowledge acquisition route.

임상간호사의 셀프리더십, 자기효능감, 조직몰입이 안전관리활동에 미치는 영향

박은희,Eun-Hee Park 한국디지털정책학회 2023 디지털정책학회지 Vol.2 No.3

본 연구는 종합병원 간호사를 대상으로 셀프리더십, 자기효능감, 조직몰입이 안전관리활동에 미치는 영향을 파악하기 위한 서술적 조사연구이다. 연구대상은 G시에 위치한 3개의 종합병원 간호사로 코로나19 펜데믹 상황임을 고려하여 온라인 설문을 실시하였다. 자료수집은 2021년 8월 20일부터 8월 30일이었다. 연구결과, 셀프리더십(r=.618, p<.001), 자기효능감(r=306, p<.001), 조직몰입(r=0518, p<.001)은 안전관리활동과 양의 상관관계를 보였다. 또한 셀프리더십, 자기효능감, 조직몰입이 안전관리활동을 38.2%로 설명하였다 (F=20.478, p<.001). 그러므로, 종합병원간호사의 환자관리활동을 높이기 위해 정기적인 리더십 교육, 자기효능감 교육 등을 실시한다면 도움이 될 것이다. 또한 조직몰입 향상을 위해 긍정적인 업무환경 조성과 간호사 업무에 대한 법적 기준 마련, 적절한 보상 체계의 마련이 된다면 환자안전관리활동에 긍정적 효과가 있을 것이다. This study is a descriptive survey study for nurses in general hospitals to understand the effects of self-leadership, self-efficacy, and organizational commitment on safety management activities. The subjects of the study were nurses at three general hospitals located in G City. An online survey was conducted in consideration of the COVID-19 pandemic situation. Data collection was from August 20 to August 30, 2021. As a result of the study, self-leadership (r=.618, p<.001), self-efficacy (r=306, p<.001), organizational immersion (r=0518, p<.001) showed a positive correlation with safety management activities. In addition, self-leadership, self-efficacy, and organizational commitment explained 38.2% of safety management activities (F=20.478, p<.001). Therefore, it would be helpful to provide regular leadership education and self-efficacy education to increase patient management activities of general hospital nurses. In addition, if a positive work environment, legal standards for nurse work, and an appropriate compensation system are established to improve organizational commitment, it will have a positive effect on environmental safety management activities.

Polar Oil 계 Emulsion 의 상거동과 안정성에 관한 연구

박은희,정흥호,김종규,김형일,노재성 ( Eun Hee Park,Hung Ho Chung,Jong Gyu Kim,Hyung Il Kim,Jae Seong Rho ) 한국공업화학회 1997 공업화학 Vol.8 No.3

Cetyl alcohol과 polyoxyethylene(20) sorbitan monooleate를 혼합한 유화제와 양친매성 resin을 용매에 용해시킨 분산상에 분산매인 물을 첨가하여 O/W 에멀젼을 제조하여 HLM(Hydrophilic Lipophilic Balance) 값과 온도변화에 따른 상변화를 관찰 하였다. 양친매성 resin을 함유한 에멀젼은 이를 함유하지 않는 에멀젼보다 온도변화에 따른 상안정성이 좋다는 것을 알 수 있었다. 또한 양친매성 resin이 에멀젼 안정성에 미치는 영향을 파악하기 위해 양친매성 resin에 함유되어 있는 작용기중 하나의 작용기를 갖는 resin을 model compound로 하여 HLB값과 온도변화에 따른 에멀전 상거동을 관찰한 결과, Poly(acrylic acid)<poly(acryl amide)<poly(ethyl methacrylate) 순으로 안정성을 나타내는 것을 알 수 있었다. O/W emulsions were prepared by adding water to the solution containing amphiphilic resin and the mixed emulsifier of cetyl alcohol and polyoxyethylene(20) sorbitan monooleate. Phase behavoir of these emulsions was studied at various HLB(Hydrophilic Lipophilic Balance) values and temperatures. The polar oil emulsion containing the amphiphilic resin showed improved phase stability at various temperatures. Model compounds which contain one of the functional groups in the amphiphilic resin were used in the polar oil phase in order to study the effect of interaction between the functional group and the emulsifier on the phase stability of emulsion. These model compound emulsions showed the phase stability order of poly(acrylic acid) < poly(acryl amide) < poly(ethyl methacrylate).

Zirconia 에 담지된 황산니켈 촉매의 특성과 산촉매성질

박은희,김선아,조해석,손종락 ( Eun Hee Park,Seon Ah Kim,Hae Souk Cho,Jong Rack Sohn ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.2

Occlusal Analysis of the Subjects with Chewing Side Preference Using the T-Scan II System

박은희,김미은,김기석,Park, Eun-Hee,Kim, Mee-Eun,Kim, Ki-Suk Korean Academy of Orofacial Pain and Oral Medicine 2006 Journal of Oral Medicine and Pain Vol.31 No.3

While orofacial pain or various dental factors are generally considered as the primary cause of unilateral chewing tendency, there exist several studies indicating that dental factors did not affect the preferred chewing side. The aim of this study was to examine difference of occlusal scheme between the subjects with and without chewing side preference. The difference between the chewing and non-chewing sides in the unilateral chewing group was investigated as well. Computerized, T-Scan II system was used for occlusal analysis. 20 subjects for the unilateral chewing group (mean age of $25.25{\pm}2.84$ years) and 20 subjects for the bilateral chewing group (mean age of $27.00{\pm}5.07$ years) were selected by a questionnaire on presence or absence of chewing side preference and those with occlusal problem or pain and/or dysfunction of jaw were excluded. T-Scan recordings were obtained during maximum intercuspation and excursion movement. The number of contact points, relative occlusal force ratio between right and left sides, tooth sliding area and elapsed time throughout the maximum intercuspation were calculated. Elapsed time for excursion was also investigated. The results of this study shows that the unilateral chewing group had the smaller average tooth contact areas compared with those of the bilateral group (p<0.005). In the unilateral chewing group, the contact areas of non-chewing side are smaller than those of chewing side (p<0.005). The contact areas on their preferred sides were not significantly different with those of right or left side of the subjects without chewing side preference. There was no significant difference in the elapsed time during maximum intercuspation and lateral excursion, the sliding areas and relative of right-to-left occlusal force ratio between the two groups. From the results of this study, it is likely that individuals prefer chewing on the side with more contact areas for efficient chewing.

설사환자에서 Campylobacter jejuni의 분리 및 항생제 감수성 특성

박은희,김정아,최성화,빈재훈,최홍식,석동희,이수철,김영희,Park, Eun-Hee,Kim, Joung-A,Choi, Seung-Hwa,Bin, Jae-Hun,Cheigh, Hong-Sik,Suk, Dong-Hee,Lee, Su-Chul,Kim, Young-Hui 한국생명과학회 2007 생명과학회지 Vol.17 No.6

2005년 1월부터 2006년 10월까지 설사환자의 분변 882건에서 27주의 C. jejuni가 분리되어 3.1%의 분리율을 보였으며, 계절별로는 7월에 가장 높은 9주(11.7%)가 분리되었으며, 분리균주 모두는 catalase 양성, hippuric acid를 가수분해하였다. heat-stable antigen을 이용한 PHA법으로 확인한 혈청형은 HS2형이 18.5%로 가장 많이 분리되었으며 Hs1/44형과 HS2l형 이 각각 11.1%, HS4 complex와 HSl9형 이 각각 7.4%,그리고 HSl8형이 3.7%였으며, 40.7%인 11주는 본 실험방법으로 혈청형을 확인할 수 없었다. 항생제에 대한 내성정도는 cephalothin에는 100%의 내성률을 erythromycin과 imipenem에는 0%의 내성률을 나타내어 분리균주 모두 감수성이었다. 항생제별로는 trimethoprim-sulfamethoxazole 63.0%, tetracycline 51.9%, ciprofloxacin 37.0%, ampicillin과 nalidixic acid 33.3%, gentamycin 25.9%, clindamycin 7.4%, chlorarmphenicol 3.7%의 순으로 내성률을 보였다. ln this study we isolated 27 isolates of Campylobacter jejuni from stool samples of 882 diarrheal patients. The seasonal distribution of patients was highest at July (11.7%). All the isolates of C. jejuni hydrolyzing sodium hippurate were serotyped on basis of heat-stable antigens, and identified with the use of passive hemagglutination assay. A total of 59.3% among 27 C. jejuni isolates were identified into 6 different serotypes, which serotype HS2, HSl/44, and HS2l were dominant. Antibiotics resistant rates of C. jejuni isolates were shown to be 100%, 63.0%, 51.9%, 37.0%, 33.3%, 25.9% and 7.4% to cephalothin, trimethoprim- sulfamethoxazole, tetracycline, ciprofloxacin, ampicillin, gentamycin and clindamycin, respectively. All isolates were sensitive to the erythromycin and imipenem.

연구논문 - 촉매 : 시리카 - 알카리 토금속 산화물 촉매의 산 및 촉매성질

박은희,손종락 ( Eun Hee Park,Jong Rack Sohn ) 한국공업화학회 1992 한국공업화학회 연구논문 초록집 Vol.1990 No.3

설사 환자에서 분리된 Staphylococcus aureus의 특성

박은희,민상기,이주현,박연경,정구영,빈재훈,Park Eun Hee,Min Sang Gi,Lee Ju Hyeoun,Park Yon Koung,Jeong Gu Young,Bin Jae Hun 한국생명과학회 2005 생명과학회지 Vol.15 No.4

부산광역시 관내 병원에 내원한 설사환자 704명의 분변으로부터 64건의 황색포도상구균을 분리하였다. 황색포도상구균의 평균 분리율은 $9.1\%$였으며, 5월에 가장 높은 16건$(16.8\%)$이 분리되었으나 계절에 상관없이 꾸준히 분리되었다. 분리된 황색포도상구균 64균주 중 $45.3\%$인 29균주의 황색포도상구균이 장독소를 생산하였으며, 장독소 유형별로 A형이 24균주$(37.5\%)$, B형이 3균주$(4.7\%)$, C형이 2균주$(3.1\%)$순이었다. 또한 15종의 항생제에 대한 내성율은 penicillin과 ampicillin에 대하여 각각 $95.4\%$와 $93.8\%$로 매우 높은 내성율을 나타내었으나, chloramphenicol과 vancomycin에 대하여는 $100\%$ 감수성이었다. Oxacillin에 내성을 나타내는 MRSA은 $31.3\%$였으며, vancomycin에 내성을 나타내는 VRSA은 $0\%$였다. 성별과 연령에 따른 MRSA의 분리율은 남자가 $35.7\%$ (15균주/42균주), 여자는 $26.3\%$(5균주/19균주)로 남자가 더 높았으며, 연령별로는 9세 이하 5명(남자 4명, 여자 1명), 20대 1명(남자 1명), 30대 2명(남자 2명), 50대 4명(남자 3명, 여자 1명), 60세 이상 8명(남자 5명, 여자 3명)이었다. 10대와 40대에서는 MRSA가 분리되지 않았다. 공시 항생제에 대한 내성양상은 분리된 64주 중 61주의 균이 최소 2종류 이상의 약제에 대하여, 최고 12종류의 약제에 대하여 내성을 나타내는 다양한 다제내성형을 나타내었다. The major causative bacteria of food poisoning were Salmonella spp. $(35.6\%)$, Staphylococcus aureus $(11.3\%)$ and Vibrio parahaemolyticus $(3.2\%)$ in our country. In this study we attempted isolation of S. aureus from stools of patients with diarrhea. Sixty-four strains $(9.1\%)$ were isolated from 704 the stools of patients with diarrhea. The enterotoxin was detected from 29 isolates $(45.3\%)$: 24 isolates $(37.5\%)$, 3 isolates $(4.7\%)$ and 2 isolates $(3.1\%)$ were A, B and C type, respectively. In the antibiotic susceptibility, 63 isolates $(98.4\%)$ were resistant to penicillin, 60 isolates $(93.8\%)$ to ampicillin, 35 isolates $(54.7\%)$ to erythromycin, 32 isolates $(50.0\%)$ to gentamycin, 22 isolates $(34.4\%)$ to tetracycline and 20 isolates $(31.3\%)$ to oxacillin. All of S. aureus isolates were susceptible to chloramphenicol and vancomycin, 20 isolates $(31.3\%)$ were methicillin-resistance S. aureus (MRSA). MRSA isolation rate was higher in male $(35.7\%)$ than female $(26.3\%)$. With the exception of two isolates which were resistant only to penicillin, sixty-one isolates were multiple antibiotic resistance.