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Development of species specific RAPD markers using populus cpDNA
Jae Soon Lee(李載順)Eun Woon Noh(盧銀雲),Suk Sung Jang(張錫成),Suk Koo Lee(李錫求),Eui Rae Noh(盧義來),Don Koo Lee(李敦求) 한국육종학회 1994 한국육종학회지 Vol.26 No.4
Chloroplast DNAs(cpDNA) from eight different species of Populus(P. alba, P. glandulosa, P. alba×P. glandulosa, P. davidiana, P. maximowiczii, P. nigra×P. maximowiczii, P. nigra, and P. Koreana×P. nigra), Salix pseudo-lasiogyne and Nicotiana tabacum were compared by PCR amplification. The cpDNAs were amplified at relatively low annealing temperature so that many bands could be observed. Amplification of a spacer between 16S γRNA gene and 23S γRNA gene located in inverted repeats in cpDNA resulted in a major band in size of 2.3 Kb and several minor bands of various sizes. A band specific to Populus was found when the amplification was done at lower annealing temperature. Amplification with primers deduced from γpoC1 and γpoC2 gene produced the major target band as well as several additional (minor) bands that could be used for the differentiation of each Populus species. The minor bands appeared repeatedly even when different thermocycles were employed. With the amplification profile, it was possible to differentiate Populus species.
Sang Don Han(韓相敦),Don Koo Lee(李敦求),Sang Yung Shim(沈相榮),Sung Ho Hong(洪性昊),Sung In Shon(孫聖仁) 한국육종학회 1990 한국육종학회지 Vol.22 No.2
Interspecific hybrids between pitch pine and some southern pines were examined on their growth and morphological characteristics. The hybrid pines included in this study were Pinus rigida×P. taeda F₁, P. rigida×P. elliottii F₁, and P. rigida×P. echinata F₁, P. rigida×P. radiata F₁ and P. rigida×P. serotina F₁, which have been grown for more than 15-year-old. P. rigida×P. taeda F₁ showed the best growth in height, diameter, mean tree volume and stand volume per hectare among all hybrid pines at all 8 locations throughout the nation. The next best hybrid pines were P. rigida×P, elliottii F₁ and P. rigida×P. serotina F₁. However, P. rigida×P. radiatea F₁. and P. rigida×P. echinata F₁ rather grew poorer than pitch pine. Growth patterns of the hybrid pines were also compared among four different locations. In Daeduck, Chungnam and Wanju, Chonbuk, hybrid pines did not show and growth retardation after 20 years. Some morphological characteristics such as, bark thickness, the number of epicormic sprout, and various needle characteristics were significantly different among hybrid pine species. The fast growing P. rigida×P. taeda F₁ had the longest needle, thinnest bark, fewest epicormic sprout and the most straight stem. The greatest nubmer of stomata per 1㎟ was shown by P. rigida×P. taeda F₁, and followed by P. rigida×P. serotina F₁, P. rigida×P. elliottii F₁, P. rigida×P. radiata F₁ and P. rigida×P. eachinata F₁ in descending order.
Phenolic compounds of some alnus species growing in Korea
Kyung Ok Jung(鄭卿玉),Don Koo Lee(李敦求) 한국육종학회 1986 한국육종학회지 Vol.18 No.4
This study was conducted to compare phenolic constituents in leaves and stem barks of Alnus hirsuta Alnus hirsuta(sex inversed), Alnus hirsuta var. sibirica, Alnus japonica, Alnus borealis, and Alnus maximowicz growing widely in Korea. The above alders were clustered using the Euclidean distance based on the phenolic composition. Eight phenolic compounds were identified in the leaves, while nine phenolic compounds in the stems. Phenolic constituents in leaves were not exactly same as those in stems. Total amount of phenolic compounds was higher in stems than in leaves. Thus, phenolic constituents differed not only among alder species but between organs.
Isolation of nod F gene from Frankia spp. symbiotic with alnus species
Min Il Choi(崔敏逸),Don Koo Lee(李敦求),Eun Woon Noh(盧銀雲),Jae Soon Lee(李載順) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
Frankia spp. are actinomycetes known to have symbiotic relationship with many actinorhizal woody species including Alnus and Eleagnus spp. Although they are known to infect those woody species by the similar mechanisms which Rhizobium and Bradirhizobium colonize legume species, most of the mechanisms concerning the nodule formation have remained unknown. In this study, we examined whether Frankia spp. have the same nod F gene as that of Rhizobium by amplification of the gene with the primers deduced from Rhizobium sequence. The amplification product and subsequent southern hybridization with nod F gene from Rhizobium meliloti confirmed that Frankia also carries the gene(or DNA segment) comparable to nod F gene in Rhizobium.