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전기로슬래그 혼합골재를 이용한 조기교통개방이 가능한 연속 적층식 상온 아스팔트포장 유지보수 공법
김완상,이석홍,권문현,최도선,Kim. Wan-Sang,Lee. Suck-Hong,Kwon. Mun-Hyun,Choi. Do-Sun 한국방재학회 2007 한국방재학회 학술발표대회논문집 Vol.2007 No.1
The main purpose of this paper is to introduce the early traffic opening-capable continuous multi-layer asphalt pavement maintenance method using electric arc furnace slag aggregate and emulsified asphalt to the actual construction sites. As well known, the previous surface treatment method of emulsified asphalt mixture have various shortcomings such as long work-time, traffic congestions, plastic deformation and poor evenness. Thus, the proposed method has enabled tile early traffic opening by utilizing the optimized emulsified asphalt after consideration of the climate and road conditions in Korea. The application of the electric furnace slag with <TEX>$6\;{\sim}\;18\;mm$</TEX> thickness is helpful to become a environment-friendly construction method. And also, it has been improved enough to accommodate night-time works of mechanized construction activities for wearing course and control course.
Efficient Regeneration from Foliage Leaf and Immature Spathe Explants of Garlic
Do Sun Kim(김도선),Yul Kyun Ahn(안율균),Jong Gyu Woo(우종규) 한국원예학회 2008 한국원예학회 학술발표요지 Vol.2008 No.12
마늘은 생장점 유래의 캘러스 배양으로 Agrobacterium을 이용한 형질전환이 어려운 실정이므로, 엽절편체 및 미숙총포 조직을 이용한 고효율 재분화 체계를 개발하고자 하였다. 난지형 및 한지형 마늘 4개 품종의 보통엽을 발아시기별, 절편크기별로 처리한 결과 2,4-D 0.1㎎ L<SUP>-1</SUP>와 BA 0.1㎎ L<SUP>-1</SUP>가 첨가된 배지에서 계대배양하는 것이 callus 형성률이 높았다. 마늘 발아 후 신초 1.0-2.0㎝ 시기의 보통엽 조직에서 체세포 유사 구조의 캘러스 형성률이 높았고, 첫 번째 보통엽 절편에서만 캘러스가 유기되었다. 이들 캘러스는 10㎎ L<SUP>-1</SUP> BA가 첨가된 배지에서 완전한 식물체로 재분화되었다. 미숙총포 절편은 NAA 1.0㎎ L<SUP>-1</SUP>, BA 1.0㎎ L<SUP>-1</SUP>의 배지조건에서 높은 재분화를 보였다. 또한 이들 식물체는 6% sucrose가 함유된 MS 기본배지에서 다수의 구를 생산할 수 있었다. Plant regeneration of garlic was successfully established from foliage leaf and immature spathe explants. The foliage leaf explants of four garlic (Allium sativum L.) cultivars were cultured on LS medium containing 0.1 ㎎L<SUP>-1</SUP> 2,4-D and 0.1 ㎎L<SUP>-1</SUP> BA with different sampling stages and explant sizes. Callus was induced from the first foliage leaf explants only. Larger sized (0.5 ㎝) foliage leaf explants were the highest in callus formation during sprouting stage of 1.0-2.0 ㎝ leaf. These calli from foliage leaf explants were very similar with somatic embryos in appearance. When these calli from foliage leaf explants were transferred to the differentiation medium containing 10 ㎎L<SUP>-1</SUP> BA, the shoots differentiated normally. Shoot regeneration and in vitro bulblet formation from immature spathe explants were rapid and efficient on MS medium containing 1.0 ㎎L<SUP>-1</SUP> NAA, 1.0 ㎎L<SUP>-1</SUP> BA, and 6% sucrose. Results indicate that immature spathes are more suitable explants for transformation than the calli from foliage leaf explants, since these tissues need longer time for regeneration and result in very low regeneration frequency.