Single Somatic Embryogenesis from Transformant with Proteinase II Gene in Panax ginseng C.A. Meyer

Yang,Deok-Chun,Kim,Se-Young,Rho,Yeong-Deok,Kim,Moo-Sung 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Ginseng(Panax ginseng C.A. Meyer) is a perennial herbaceous plant which grows very slowly. It takes about 3 to 4 years from seeding to collecting the ripe seeds and the ginseng propagation is very difficult. and so, it is very difficult to breed ginseng plant. Ginseng tissue culture was started from at 1960, and ginseng commercial product by in vitro callus culture was saled, however upto now, regenerants were not planted to soil normally. Recently, plant genetic engineering to produce transgenic plants by introducing useful genes has been advanced greatly. In a present paper, transformation of ginseng plants was achieved by co-cultivation with Agrobacterium harboring the binary vector coding Proteinase-II gene, which confer resistant or tolerant to insect pests, The binary vector for transformation was constructed with disarmed Ti-plasmid and with double 35S promoter. The NPT II gene and introduced genes of the transgenic ginseng plants were successfully identified by the PCR. Especially the transgenic ginseng plants were regenerated using new techniques such as repetitive single somatic embryogenesis.

홈 페이지의 시각적 표현에 관한 연구

전양덕,금진우 서울産業大學校 1996 논문집 Vol.43 No.1

The internet as an international network today attracts concerns of many people. In the meantime internet has given and received data in the professional field in part only as text type however, now it provides with variety to the moving animated image and to service of various sounds from a splendid picture by entrance of the worldwide web (WWW). Since the various type data is supported as these the user's interface that can see the data has also changed. Since Web Browser has developed many people began to gather in internet connection and naturally the pretty and splendid programs began to enter on the internet. As the commercial use of internet is permitted many of enterprisers or businessmen started to make their home-page as well as individuals. In such a timepoint this study is to check the principle of visual expression from the viewpoint of participation and roles of professional design in this new field in order to provide the information more effectively by getting rid of reckless generation, providing and search.

Induction of Ginseng Hairy Roots And Their Possible Application To Large Scale Culture

Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Ginseng(Panax ginseng C. A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng hairy roots induced by introducing Ri-plasmid of Agrobacterium rhizogenes into genomic DNA of plant cells show vigorous growth, and the hairy roots produce the same or more saponins than natural ginseng roots. Therefore, hairy roots can be used for commercial purposes. The present study was carried out to induce hairy roots with both active growth and high saponin contents. Numerous hairy roots of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes R1000 A4T in dark condition after one month of culture. About 3 hundred lines of hairy roots were selected according as morphological characters on medium with carbenicillin. After pre-selection of fifteen lines of hairy roots with active growth, KGHR-l and KGHR-8 lines were finally selected which had characters of high content of ginsenoside-Rd and ginsenoside-Re, respectively. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark (22℃) under 60 rpm gyratory shaking. Hairy roots grew well in 5L Erlenmeyer flasks, lL roller drums, 10L jar-fermenters, and especially in 20L air-lift culture vessels.

産業디자인에 있어서 포스트모던 傾向에 關한 硏究 : Concentrated on Graphic Design 그래픽 디자인을 中心으로

全洋德 三陟大學校 1992 論文集 Vol.25 No.-

The Post-Modernism, of which the mental basis is originated in France, Germany and also in America at 1960'S to the field of Art, Music, spread fast throughout all over the Western. The term 'Post-Modernism' first used by Arnold Toynbee right after World WarⅡ means new Avan-garde against Modernism that attained the power of new tradition. Joseph Hudnut, however, used first in the full in the full sense of the term in 1949 and began to go current in Design after Charies Jencks used in his book " The Language of Post-Modernism Architecture', Rosemarie Tissi applied it the Graphic Design for the first time. Ihab Hassan, the enthusiastic supporter of Post-Modernism, defined the characteristics as follows' 1. Indeterminacy 2. Fragmentation 3. Decanonization 4. Unrepresentability 5. Hybridization 6. Populism 7. Performance and Participation 8. Immensity Also, the characteristics of Post-Modernism in Desigh are following; 1. Historicism 2. Metaphor and Symbolism 3. Ornamentation 4. Dual Coding 5. Complexity and Contradiction 6. Conctextualism 7. Electicism And the expression of Post-Modernism characterized as follows; 1. Layering 2. Superscale 3. Supermannerism 4. Supergraphic 5. Overlapping When we recognize the fact that Post-Modernism regained the historical view which Modernism abandoned and focused on the public's concentration, it can be the first step toward the Design of these days of nationalism, democracy which we aim to. Therefore, we have understand Post-Modernism in general not as a method only for fixing the form, but as a New Design method based on the new perception of human beings.

Agrobacterium tumefaciens C58에 依하여 形質轉換된 人蔘組織에서의 saponin 生産

梁德春,李政明 경희대학교 부설 식량자원개발연구소 1988 硏究論文集 Vol.9 No.-

The present study was conducted to obtain some basic information on the transformation of ginseng tissue, identification of opine compound and protein, and saponin production from ginseng tissue transformed with pTiC58. Ginseng tumor callus induced by pTiC58 could be continuously cultured on the phytohormone-free basic medium. The transformation was reconfirmed by the detection and identification of opine compounds from the tumor callus. The transformed ginseng calli contained higher amount of protein than normal calli and the protein pattern of transformed calli was quite different from that of normal callus. Higher saponin content was found in the transformed callus than the normal callus, and ginsenoside composition of the transformed callus was similar to that of cultivated ginseng root, but quite different from that of normal callus.

디자인價値로서의 크리에이티비티에 關한 硏究 : 廣告디자인을 中心으로 focussed on Advertising Design

全洋德 서울産業大學校 1994 논문집 Vol.39 No.1

Value means important or sense that an object has, so that a design value also must mean importance or sense that a design possesses. Therefore, the creation of a valuable design is the fundamental goal that a design should have, and also it is the basic guide line which suggests the direction that the design should follow. In the past, a design has been recognized as a concept that it is produced by applying pure arts to the industry. The more complicate the society is, the more difficult the creativity in a design is because it needs a very special technique. Thus the design methodology is attempted to solve the problem. The most important thing in advertising design is to extract a creative concept by U.S.P.(unique selling point). It is the creativity in a design value. The advertising creativity is different from the pure art creativity in that it has a commercial purpose as well as an esthetic creation. Therefore, a design should refer the various social elements with creativity to produce a real valuable advertising design. This is the design methodology which creates good design.

Simple Sequence Repeat Primer에 의한 딸기의 Genomic DNA 분석

양덕춘,최성민,김무성,민병훈 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-

본 연구는 진핵세포에 널리 산재되어있는 repeat sequence를 primer로 사용하여 딸기의repeat sequence 좌위를 증폭시켜서 분석하고 이를 같은 종일지라도 지역간의 차이로 인한 환경의 변화 또는 재배방법의 차이에 의해서 일어나는 변이를 분석하는 방법에 활용할 수 있도록 하기 위하여 수행하였다. GGC repeat sequence의 딸기 genomic DNA에 존재여부를 판명하기 위하여 (GGC)_(6) probe를 DIG을 사용하여 labeling하여 딸기 genomic DNA에 hybridization한 결과 확실한 spot이 나타났다. 또한 repeat sequence로 이루어진 UBC SSR primer 100개중에서 4가지 서로 다른 primer에서 선명한 band를 확인할 수 있어서 딸기의 genomic DNA내에 여러 종류의 repeat sequence가 있는 것으로 판단되었다. 따라서 각 품종별 표지로서 이용성을 알아보고자 PCR을 수행하였는 바 (ACC)_(6)와 (CCG)_(6)의 primer에서는 다른 band에 비해 매우 확실한 밴드를 형성하였다 이러한 PCR product의 염기서열분석을 위해 우선 대량증식을 위한 PCR의 적정 조건은 primer 10p㏖, DNA 50ng으로서 수행하는 것이 DNA의 증폭에 좋았다. 증폭된 1 Kb DNA 절편을 이용하여 염기서열을 조사하였던 바, 808bp의 서열을 알아냈고 그 반복염기서열은 효모와 사람에 존재하는 유전자이었으며 Cdontella sinensis와의 유의성 검정결과 ACC 반복염기에 62% 동일성을 나타내었다. This study was carried out to investigate genetic variation caused by environmental change or cultural method difference, which was performed by amplifying repeat sequence loci of strawberry widely dispersed in eukaryotic cell. DIG labeled(GGC)_(6), probe was used to test if GGC repeat sequence exists in strawberry genomic DNA, resulting in clear spot on the filter. Among the 100 UBC SSR primers which contain repeat sequence, four different primers amplified clear DNA fragments, suggesting that the repeat sequence exist in the genome of strawberry. PCR was also performed to test whether these primers could be used for a marker among strawberry cultivars. (ACC)_(6), (No. 861) and (CCG)_(6) (No. 865) primers showed very clear bands compared to the other primers. The amplified 808 bp of 1 kb DNA was sequenced, and database search using the sequence information showed that the repeat sequence was the gene existed both in yeast and human, and a significant sequence homology to Odontella sinensis was found.

Ti-plasmid에 依하여 形質轉換된 煙草組織의 特性과 再分化 樣相

梁德春,李政明 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

Studies were conducted to examine the characteristics of teratoma shoots obtained from diploid and haploid tobacco crown gall tumor tissues induced by the inoculation of Agrobacterium tumefaciens and the effects of plant hormones and active carbon in the cultural media on the growth and regeneration patterns of the teratoma shoots. Results can be summarized as follows. 1. Teratoma shoots could be obtained when the crown gall tumor, induced by the inoculation of A. tumefuciens strain C-58 to the stem, was successively grown on hormone-free medium. 2. The growth of tumor callus induced by C-58 strain was inhibited when several plant hormones were added to the cultural media. Plant regeneration could not be obtained from the tumor callus or the teratomas. 3. Addition of active carbon and benzyl adenine to cultural media was effective for shoot elongation and reduction in multiple shoot formation. 4. The growth of tumor callus obtained from the haploid plant was promoted by the addition of plant hormones to the media. Whole plants were also regenerated from the teratoma shoots formed from tumor callus. 5. Normal or transformed plants were regenerated from the tumor callus formed by the inoculation of a strain of A. tumefaciens collected from field soil. The transformed plants are characterized by having twisted dark-green leaves, shorter internodes, and abundant axillary shoots.

시각 기호로써 ICON에 관한 연구 : 디자인의 원칙과 평가 방법을 중심으로 Around the principle of design and assessing method

전양덕,금진우 서울産業大學校 1996 논문집 Vol.43 No.1

As the computer has popularized among the masses the computer environment also follows to need of the masses. As the delivery method of all messages changes to symbolic method using graphic from the printing type as the majority the window with diversified from to provide GUI(Graphic User Interface) environment in the computer field closed up on stage. The appearance of such GUI manner has provided footing ground to contribute by the graphic designers in the computer science field. This is that has provided another expressive method to the designers but is not solved desire of computer users. Since therefore, in order to grope for solution of numerous problems encountered when designing the ICON a part of the informational design, there is need to compose the information scientifically by applying the principle and knowhow of visual design in addition to make design the interface by the graphic designers. This study has groped for the assessing method of designed ICON as well as the principle and method of visual design may be applicable when designing the ICON.

시각예술로서의 인터넷 광고디자인과 기호학적 접근

전양덕 서울産業大學校 2005 논문집 Vol.54 No.4

There is no doubt that Humanbeings are surrounded by Sign in their everyday life. In either accidental or necessary way, Sign has already formed common ground with human life. In this regard, the study on Sign, which is called Semiotics, will surely serve as groundwork for other study including Communications. This is why some people do not hesitate to call Semiotics as one of the main 3 basic study, together with Philosophy that studies human thoughts and Psychology that does human mental structure. Likewise, Semiotics helps designers anticipate the way meaning is created, train their both analytic and esthetic insight. Consequently, it features its ability to lead designers to those of morecreativity. Until recently, studies in the advertising area have been mainly about Social Science Paradigm, such as Economics, Marketing, Communications, Psychology. However, Modern broad and complex communication setting claims that current attempt to test the message delivery in practical and statistical measure can bear huge chance of error. In other words, when it comes to the message, what is the most important is not how correctly it has been delivered but how it actually works as a text and affects. While texts in books are so linear, sequential, and concrete that reflects designers' intention only, hypertexts are so non-linear that users or visitors can search for their own meaning randomly by editing and analyzing the texts on their own. Likewise, Semiotics gives birth to the study on the way meaning and value are created through presence, and to the scientific and logical creativity that is contrast to the intuition-oriented design or its analysis. Among diverse Semiotical approaches including multimedia advertising design on internet setting, humor can be the most similar concept to Humanism by Semiotic standard. Recent study shows that humor is the most effective way to boost click rate on commercial banner on websites which are also sort of hypertext. Another effective way to create humorous message or visual humor will be a thorough understanding of 'pun' concept, which utilizes tricks on homonym. Accordingly, Semiotic methodology that helps create diverse meanings out of fixed text can surely be the necessary approach toward creativity of the multimedia advertisements in this new era on Internet.