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RISS 인기검색어
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- 저자 : Dao Chull Shin(申斗澈) (검색결과 1 건)
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In Youl Baek(白寅烈),Yong Hwi Yoon(尹用輝),Dao Chull Shin(申斗澈),Gyu Hwan Park(朴圭煥),Young Hun Hwang(黃永鉉),Dal Ung Kim(金達雄) 한국육종학회 1997 한국육종학회지 Vol.29 No.3
Effective conservation and the use of plant genetic resources are essential for future agricultural progress. In this study, the genetic relationships among fourteen species of Glycine were analyzed based on the RAPDs. A total of fourteen species (twenty one accessions) in the genus Glycine were identified by the frequency of RAPDs polymorphisms. Ten primers out of thirty primers tested, were selected for the reliability and repeatability of banding patterns. The amplification of the genomic DNA produced eighty two polymorphic band patterns with the average of 8.2 bands per primer. By Nei and Li’s formula, the mean 1-F value (genetic similarity) was 0.461, and the highest value was 0.774 (G. max : G. microphylla) and the smallest one was 0.128 (intraspecies G. max). That of interspecies in the subgenus Glycine was 0.620 and 0.250 in the highest and the lowest cases, respectively. Thus the genetic similarities were much variable within the interspecies of subgenus Glycine but those were somewhat small between the subgenus Glycine and Soja. By UPGMA (unweighted pair group method using an arithmetic average) cluster analysis based on 1-F value, the fourteen species of Glycine could be classified into four major-groups (genetic similarity (GS) : 0.45) and nine sub-groups (GS : 0.35), that is, into Group Ⅰ : subgenus Soja : G. max, G. sofa, G. gracilis; Group Ⅱ : G. falcata ; Group Ⅲ : A : G. latrobeana, G. canescens, G. microphylla, B : G. curvata, G. cyrtoloba, C : G. clandestina, D : G. tabacina(2n=80) ; Group Ⅳ : A : G. arenaria, B : G. tomentella (2n=38, 40, 78, 80), C : G. tabacina (2n=40, 120), G. latifolia. By multidimensional scaling (MDS), the subgenus Glycine and Soja were clearly classified in the clustering of genetic relationship distance. The genetic relationship distance was closely identified between G. max of the cultigen and G. clandestina, G. tomentella, G. cyrtoloba, G. curvata of the subgenus Glycine.
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