경주마(競走馬)의 혈액화학성상(血液化學性狀)에 관한 연구: I. 혈청효소(血淸酵素) 활성치(活性値)

강정부,Kang, Chung-boo 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1

The present study was carried out to investigate the serum enzyme activities of racehorses. The enzymes investigated were aspartate and alanine aminotransferase (GOT, GPT), ${\gamma}$-glutamyl transpeptidase(${\gamma}$-GTP), Lactic dehydrogenase(LDH), creatine phosphokinase(CPK), and alkaline phosphatase(ALP). Animals used were 30 healthy racehorses(♀17, ♂13) average weighing 435kg and were from 2 to 8 years of age. LDH activity was progressively decreased with age and next in the order of GOT and CPK activities. GOT and ${\gamma}$-GPT activities were not changed with age but ALP activity tended to be decreased wish age. Activities of GOT, GPT, ${\gamma}$-GTP and ALP were higher in female than in male. There was no difference in CPK activity by sex, and no difference was found out among breeds.

건유(乾乳)와 필유(泌乳)에 따른 유우(乳牛)의 혈액(血液) 및 혈액화학상(血液化學像)의 변화에 관한 연구

강정부,이국천,이순선,조희택,안동원,Kang, Chung-boo,Lee, Kuk-cheon,Lee, Soon-sun,Cho, Hee-taek,Ahn, Dong-won 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4

This experiment was carried out to investigate the hematological and blood chemical values in dairy cattle during the dry and lactating period. Blood was collected from six healthy dairy cattle in Gyeongnam province. Leucocytes count, hemoglobin concentrations and the percentage of packed cell volume were lowest at the early lactation period. Leucocytes count was not affected during dry the to lactating period. The differential count of eosinophilic leucocytes was low at the late lactation period, while the basophilic leucocytes was high at the dry period. Inorganic phosphorus value was below under normal level. Creatinine value was from 1 to 2mg/100ml of serum. Positive reaction to CRP was shown in normal dairy cattle. The values of AST and ALT were higher during the dry than lactating period. r-GTP and total cholesterol tend to be decreased during the lactating period. CPK value was not affected for dry and lactating period.

Enzyme immunoassay(EIA)에 의한 소의 progesterone 측정과 이의 응용에 관한 연구 II. Progesterone 측정에 대한 효소면역측정방법(酵素免疫測定方法)의 확립

강정부,신종욱,최상용,Kang, Chung-boo,Shin, Jong-uk,Choe, Sang-yong 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, enzyme conjugate and gelatin were invested. The sensitivity, recovery rate and reproducibility by this assay were also analyzed. The results obtained were as follows: 1. The suitable supplementation level of gelatin was 0.2%. As the gelatin level increased to 1%, coefficient variation of interassay was shown to be irregular. 2. The optimum dilution rate of enzyme conjugate was 30 times. Enzyme activity was greatly fluctuated depending on the minor condition of enzyme conjugate technique. Therefore, it was considered to be checked when determined. 3. The sensitivity of the assay was 12 pg/tube. 4. Recovery rate was decreased when the amount of sample was too little or too much, but the recovery rate was high as 97.8% when the amount of sample between 50 and $200{\mu}l$. 5. Mean intra-assay and inter-assay coefficient of variation was 4.5% and 5.9%, respectively. By using liquid phase double antibody enzyme immunoassay, progesterone in plasma can be detected, and also this assay system is applicable to study on physiological function of progesterone and to diagnosis of reproductive disorders.

경주마(競走馬)의 혈액화학성상(血液化學性狀)에 관한 연구 II. 혈액화학치(血液化學値)

강정부,Kang, Chung-boo 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

This experiment was carried out to provide the data of blood chemistry in use of application to clinical diagnosis in racehorses. Blood was collected from 30 racehorses(♀ 17, ♂ 13). Calcium, inorganic phosphorus, blood glucose, blood urea nitrogen, cholesterol, bilirubin, fibrinogen and ketone body in serum were analyzed as follows. 1. The values of calcium and inorganic phosphorus were maintained a certain level without much variation regardless of sex and species, Ca/P ratio was in the range of 3.13~3.35. 2. The value of blood glucose was 60 mg/100ml, Age and sex of racehorses make no difference in the value. 3. The value of blood urea nitrogen and total cholesterol showed a tendency to increase according to the age. Total cholesterol value was somewhat higher in male than in female. 4. The value of total bilirubin showed a tendency to increase according to the age. There was no difference between sexes in the value. 5. The value of fibrinogen showed no apparent difference with age and sex. 6. Ketone body was negative in all of the cases. 7. There was no variation in the species of the racehorses.

Enzyme immunoassay(EIA)에 의한 소의 혈중 progesterone 측정과 이의 응용에 관한 연구 I. 이항체(二項體)의 최적조건에 관한 연구

강정부,신종욱,최상용,Kang, Chung-boo,Shin, Jong-uk,Choe, Sang-yong 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was $10{\times}10^3$ to $15{\times}10^3$, 20 and $1{\times}10^3$ times, respectively.

저(低)Calcium혈증(血症)에 관한 심전도학적(心電圖學的) 연구(硏究)

강정부,Kang, Chung-Boo 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.1

Experimental hypocalcemia was induced in normal goats by intravenous infusion with various concentration of $Na_2$-EDTA solution. 1. Progressive depression of reflexes and body temperature, paresis, and cardiac arrest were observed in two groups infused with 4% and 8% EDTA solution, whereas paresis and cardiac arrest were not observed in 3% EDTA group. 2. The patterns of electrocardiogram were changed according to the decrease of plasma Ca level in all of 7 goats. When plasma Ca decreased to 6mg per 100ml or below, sinus bradycardia or tachycardia and prolongation of QT interval were showed, while atrioventricular block was noted in case which the plasma Ca level decreased to 4mg per 100ml or below. Fibrillation and cardiac arrest were evident in case which the plasma Ca level decreased to as low as 3mg per 100ml.

Electromyographical Studies on Experimental Hypocalcemia in Goats

강정부,Kang, Chung Boo The Korean Society of Veterinary Science 1979 大韓獸醫學會誌 Vol.19 No.2

저(低)Ca혈증(血症)의 주징(主徵)인 기립불능(起立不能)의 발현원인(發現原因)의 추구를 위해 실험적(實驗的) 저(低) Ca혈증(血症)에서 기립불능(起立不能) 나아가 심장운동정지(心臟運動停止)에 이르기까지의 과정을 근전도학적(筋電圖學的)으로 실시한 결과의 내용을 요약하면 다음과 같다. EDTA 주입전(注入前)의 정상기립시(正常起立時)에는 항중력근(抗重力筋)에만 tonic discharge가 얻어졌으나 혈정(血中) Ca 농도(濃度)의 저하정도에 따라 근방전(筋放電)이 달라져 6mg/100ml 전후에서 항중력근(抗重力筋) 및 그의 길항근(拮抗筋)에도 phasic discharge를 수반한 tonic discharge가 동기적(同期的)으로 나타나 이의 현상은 후구탈력(後軀脫力)에 의해 주저앉을 때에도 마찬가지였다. 기립불능(起立不能)의 상태에선 clonic convulsion양(樣)의 근방전(筋放電)이 폐사시까지 계속되었다. 경막외마취(硬膜外痲醉)에 의한 근전도(筋電圖)에선 근방전(筋放電)은 항시 상반적(相反的)이었고 후구마비(後軀痲痺)에 의한 횡와위(橫臥位) 상태에선 어떤 근방전(筋放電)도 얻어지지 않아 EDTA 주입(注入)에 의한 저(低) Ca혈증시(血症時)와 경막외마취시(硬膜外痲醉時)의 기립불능(起立不能)의 본질(本質)은 상위(相違)한 것으로 판명되었다. 또한 저(低)Ca혈증시(血症時)에 수반되는 각종 반사 및 임상증상의 추이(推移)로 저(低)Ca혈증시(血症時)에 수반되는 기립불능은 말초신경계 및 신경일근접합부(神經一筋接合部) 및 근(筋) 자체의 기능저하보다는 중추신경계의 기능저하에 의한 가능성을 크게 시사하는 것으로 보아진다.

Progesterone의 단크론성 항체에 관한 특성 및 활용에 관한 연구 II. ELISA 기법의 개발

강정부,김종수,Kang, Chung-boo,Kim, Jong-shu The Korean Society of Veterinary Science 1991 大韓獸醫學會誌 Vol.31 No.4

Progesterone의 단크론성 항체를 생산, 이용하여 감도가 높으면서도 신속히 측정할 수 있는 ELISA 기법을 처음으로 개발코져 실시하였다. 단크론성 항체는 종래의 면역방법에 의해 획득한 항혈청에 비해 약 10배의 결합율을 보였고 titer 역시 높았다. Dot-blot 분석 결과 단크론성 항체는 IgM이었다. 경합반응은 2시간으로 충분하였고, progesterone 표준용액을 이용한 표준 곡선은 0~1000pg/well에서 거의 직선적이었다. Progesterone의 단크론성 항체를 이용한 ELISA는 임상적으로는 물론 연구용으로도 신속한 항체의 기능 측정에는 물론 각종 번식 관련의 지표로 충분히 활용될 수 있을 것으로 판단된다. This experiment was carried out to develop a sensitive, rapid, solid-phase microtitre plate assay of progesterone using the monoclonal antibody to this hormone. Monoclonal antibody to progesterone was much higher titre and binding affinity about 10 times than conventional polyclonal antibody to progesterone. Dot-blot analysis of monoclonal antibody revealed a single precipitation band when reacted with anti-mouse IgM and anti-mouse K. A competitive reaction was used with a reaction time of 2 hours. The standard dose-response curve was linear through 1,000pg/well. This ELISA system approach is applicable to evaluation for the rapid assessment of luteal function and reproductive status in both clinical and research in a wide variety of species.

소의 조기임신진단 kit의 개발 1. Progesterone의 항체생산(抗體生産) 및 항(抗) BSA항체(抗體)의 제거

강정부,이효종,최상용,Kang, Chung-boo,Lee, Hyo-jong,Choe, Sang-yong 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2

Most progesterone enzyme immunoassays(EIA) are used liquid phase double-antibody separation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of there several problems, we were prompted to develop an effective EIA system by the use of higher titer of progesterone antiserum free of anti-bovine serum albumin antibodies (anti-BSA). The results obtained were as follows. 1. The antibody of progesterone antiserum was high as $1.5{\times}10^5$. 2. Percent activity bound of progesterone antiserum was about 77 at a dilution to $5{\times}10^3$ times. 3. Progesterone antiserum was contained a large amount of anti-BSA antibodies. 4. The anti-BSA was completely absorbed by using of polymerised BSA. 5. The molecular weight of albumin polymer (polymerised BSA) obtained by using 2.5% glut. araldehyde was $5{\times}10^5$.

Progesterone의 이화(異化)효소, 20α-hydroxysteroid dehydrogenase의 활성 및 활용에 관한 연구 1. 20α-hydroxysteroid dehydrogenase의 활성 및 혈청 progesterone 농도의 변화

강정부,곽수동,Kang, Chung-boo,Kwak, Soo-dong 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

Progesterone catabolizing enzyme, the enzyme $20{\alpha}$-hydroxysteroid dehydrgenase($20{\alpha}$-HSD) is pivotal in the regulation of ovarian function in rodents, which catabolizes progesterone into biologically inactive $20{\alpha}$-hydroxypregn-4-en-3-one($20{\alpha}$-OHP). In this study was carried out the influence of $20{\alpha}$-HSD activity of ovarian function, we investigated changes in ovarian cytosol $20{\alpha}$-HSD activity and serume progesterone concentration during the estrous cycles and pregnancy in rat. During the estrous cycles, the $20{\alpha}$-HSD activities were highest on the progestrous, but serum progesterone concentration was lowest on this phase. During the pregnancy, the $20{\alpha}$-HSD activities were relatively higher early pregnancy(day-1-3 gestation) and late pregnancy(day 21 to parturition), serum progesterone concentration was maintained significantly high to day 19 of gestation. The $20{\alpha}$-HSD activities were lower during the middle pregnancy. From these results, ovarian $20{\alpha}$-HSD activities may possibly act as physiologically very important in the control and maintenance of estrous cycles in rat.