고려인삼의 주아 발달경로

정찬문(Chan-Moon Chung),임흥빈(Heung-Bin Lim),이이(Yi Lee),정열영(Youl-Young Chung),전병록(Byeong-Rok Jun) 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.3

본 시험은 인삼의 채종종자의 조직을 관찰하여 잠아의 실체를 조사하였고 묘삼의 잠아특성을 산지별로 조사하여 다경이 되는 잠아의 발달경로를 알아보았다. 그리고 묘삼을 본포에 이식하여 3 년생을 채굴하여 4 년생이 될 주아의 생성경로를 조사하였다. 묘삼의 주아는 발아종자의 경근천이부에서 피층조직이 융기하여 돔 (dome)형태의 원잠아 또는 측잠아가 형성된 후 이들 조직이 분화하여 발달하였다. 그리고 묘삼의 단뇌는 90%가 원잠아에서 유래하였으며 쌍뇌는 원잠아와 2개의 측잠아 중 1 개가 발달되어 형성되었으며 3 뇌는 원잠아와 측잠아 2 개가 발달하여 형성되었다. 본포에서 2 년생과 3 년생은 주아가 단뇌로 형성되어 있어 2 경 개체가 발생하지 않으나 4 년생은 3 년생에서 발달한 쌍뇌 이상의 주아가 있어 2 경 개체이상이 관찰되는 최초의 연생이었다. 4년생의 주아는 종자에서 형성된 원잠아, 측잠아와 2-3년생에서 뇌두부위에서 형성된 피층잠아에서 유래하는 것으로 고년생으로 갈수록 쌍뇌 또는 3뇌는 원잠아에 비해 피층잠아 유래의 주아가 많았다. The researchers studied the composition of latent buds by surveying the embryo of harvested seed, and the developmental pathway of latent buds by analyzing the characteristics of local ginseng. One-year-old ginseng seedlings were transplanted into the field and harvested two years later. The developmental pathway of the main bud, which would be the shoot of ginseng in the fourth year, was also investigated. The main bud of the seedling was formed from the region between root and shoot of the germinating seed. Primary and axillary latent buds protruded in a dome-shape from the cortex and separated from the main bud. Ninety percent of the single main bud was derived from the primary latent bud. Twin main buds were derived from a primary latent bud and one axillary bud, and triple main buds were derived from primary latent bud and two axillary latent buds. In the field, the researchers could not find 2-stem plants in 2-3 years old plants. However, the researchers found a 2-stem plant in a 4-year-old plant because its two main buds developed from a 3-year-old plant. We can conclude that a 2-stem plant was observed in the plant that was at least 4 years old. The main buds of the 4-year-old plant were formed at the primary and axillary latent bud of seed and cortex, the latent bud of rhizome in a 2-3 year old plant. In older plants, twin and triple main buds were derived mostly from the cortex latent bud than the primary latent bud.

인삼에 있어 단경 및 다경개체의 잠아형태별 수량 및 홍삼 품질 비교

정찬문(Chan-moon Chung),신주식(Ju-Sik Shin),정열영(Youl-Young Chung) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.3

인삼에 있어 다경 및 잠아 발생형태가 원료삼 및 홍삼 품질에 미치는 영향을 구명하고자 지하부 특성 및 품질요인을 조사하였다. 단경개체는 1-3개의 잠아를 갖는 형태이고 2경개체는 2-3개 그리고 3경개체는 3개의 잠아를 갖는 형태이었다. 또한 단경개체는 62.9% 그리고 2경 이상의 다경개체는 37.1%의 분포를 나타냈다. 원료삼의 등급은 단경개체에 비하여 다경개체가 될 수록 저하하였고 단경개체와 다경개체 모두 잠아수가 많은 개체에서 등급이 낮았다. 경수에 따른 홍삼수율은 대체로 30% 내외로 단경이나 다경개체간에 큰 차이가 없었다. 그러나 본삼수율은 다경개체로 갈수록 감소하는 경향이었다. 홍삼 등급은 다경개체에 비해 단경개체가 양호하였으며 다경개체는 양삼과 잡삼이 많았다. 그리고 잠아수가 많을수록 홍삼등급은 저하하였다. This study was carried out to study the effect of emergence type of multiple stem and main bud on the quality of fresh and red ginseng in Korean ginseng. To achieve the aim of this study, characteristics of roots and quality factors were investigated. Single stem plants were 62.9% of the total samples, and the remainder(37.1%) were multiple stem plants. The number of stems affected considerably on root weight. Root weight of triple stem type with triple main bud was the largest among the types. As the number of stem per plant increased, the root quality became worse. In both single and multiple stem, the more the number of main buds was, the lower the quality grade was. Yield of the red ginseng was about 30%, showing little difference between single stem and multiple stem plant. As the number of stem increased, yield of Bonsam decreased. The quality grade of red ginseng of single stem was better than that of multiple stem. Multiple stem plant produced relatively more Yangsam and Japsam. As the number of main bud increased, the quality grade of ginseng decreased.

[학생활동] 사이트(SA-YIT) 여주대학 자동차 연구회

정찬문(Chan Moon Chung) 한국자동차공학회 2002 오토저널 Vol.24 No.2

소재로 승부거는 정보산업 / 반도체 초미세가공용 포토레지스트 재료

정찬문(Chan Moon Chung) 한국공업화학회 2000 공업화학전망 Vol.3 No.5

N/A

人蔘 胚培養에서 器官分化 類形別 組織 形態的 特性

Chan Moon Chung(鄭燦文),Je Yong Kang(姜齊瑢),Jae Seong Jo(曺在星) 한국육종학회 1995 한국육종학회지 Vol.27 No.3

These studies were conducted to obtain the propagation method from the embryos of the Korean ginseng cv. violet-stem var. (Panax ginseng C.A. Meyer) There were five types of the shoot formations in the ginseng embryo culture and those are the adventitious bud from the epicotyl, the direct shoot formation from the cotyledon, the shoot primordium initiated from cortex of cotyledon, the adventitious shoot from callus and the somatic embryogenesis. In the case of the shoot formation from the epicotyl, the adventitious buds were initiated from a number of the meristematic tissues at the basal part of epicotyl and consequently developed to multi-shoots. However, in callus tissue, an embryo developed from an individual somatic cell in the callus mass by the somatic embryogenesis. The cotyledon and the roots were developed from epicotyl and hypocotyl of the embryo but the stem and the leaves were not differentiated from the cotyledon developed from somatic embryo.

인삼 조직배양에 있어 CO₂ 처리가 식물체 분화 및 생육에 미치는 영향

정찬문(Chan Moon Chung),배길관(Kil Kwan Bae),Masatoshi Aoki 한국약용작물학회 2000 한국약용작물학회지 Vol.8 No.1

This experiment was conducted to investigate the effects of length of storage period under low temperature, CO₂ enrichment and addition of plant growth regulators in Murashige and Skoog medium on the plant regeneration of Korean ginseng (Panax ginseng C.A. Meyer). Seeds were treated for 60 and 80 days respectively under 5℃ environment. 2500ppm of CO₂ was enriched by ventilation. Three plant growth regulators added to the medium were Indolbutyric acid, Benzyladenin and Gibberellic acid (GA3). The result indicated that : The capacity of differentiation was higher in the aged cotyledons from the seeds treated for 80 days under low temperature condition than in those treated for 60 days. CO₂ enrichment had stimulating effects on the growth and development of shoot primordium significantly but less effects on the formation of adventitious buds. From one zygotic embryo hundreds of plantlets were differentiated. CO₂ enrichment had effects on the formation of both indirect somatic embryo and direct somatic embryo. Indirect somatic embryo showed little growth and differentiation, being undifferentiated vascular stele and epicotyl. Direct somatic embryos were formed on the epidermis of backside basal part of cotyledon. Those embryos developed to whole plant having latent bud.

인삼 조직배양에서 CO₂ 처리가 multi-shoot 분화 및 사포닌 함량에 미치는 영향

정찬문(Chan Moon Chung),배길관(Kil Kwan Bae) 한국약용작물학회 1999 한국약용작물학회지 Vol.7 No.4

This experiment was conducted to study the effect of CO₂ (0, 2,500, 5,000, 10,000ppm) enrichment by enabling ventilation on micropropagation of multi-shoot and on the saponin contents in vitro in Korean ginseng(Panax ginseng C. A. Meyer). Embryo was cultured in Murashige and Skoog medium added 3㎎/ℓ of Indolbutyric acid, Benzyladenin and Gibberellic acid (GA₃), respectively. CO₂enrichment had little effects on the number of adventitious buds and shoots originated from adventitious buds. The ratio of differentiated shoots to adventitious buds were about 50% in CO₂enrichment treatment. The shoots originated from adventitious bud showed more rapid growth and had larger leaf area than the shoots originated from the leaf primordia did. The number of shoot primordia was the highest in 2,500ppm of CO₂enrichment treatment. On the contrary, 10,000ppm of CO₂ enrichment made smaller the number of shoot primordia and ratio of shoots to shoot primordia. The range of shoots differentiated was from shoot primordia were 15.4 to 23.9. The rate of dry weight of cultured shoots showed lowest(7.5%) in control and highest(8.59%) in 2,500ppm of CO₂ enrichment. Rate of in vitro flower in control was 7.6% and that in 2500ppm of CO₂ was about twice (15.7-16.3%) as much as in control. Flower number per a embryo cultured was about 1.2-1.3. In the multi-shoots with callus enriched by 2,500ppm of CO₂, the contents of crude saponin and ginsenosides in multi-shoots alone were higher than in multi-shoots with callus. The characteristics of ginsenosides in multi-shoots were especially the higher content of ginsenoside Rd, Re, and Rg₁.

生長調節物質이 人蔘 胚培養에 있어 器官分化에 미치는 影響

Chan Moon Chung(鄭燦文),Yo Tae Kim(金鐃,泰),Jae Seong Jo(曺在星) 한국육종학회 1989 한국육종학회지 Vol.21 No.2

This study was conducted to investigate the effect of phytohormones, IBA, GA, kinetin and BA on regeneration of plantlet, morphological characteristics of inflorescence development, growth of explant and saponin content in embryo culture of Panax ginseng. For the induction of adventitious buds and regeneration of plantlet from adventitious buds, GA was more effect than IBA, kinetin, or BA. The plantlet regeneration and inflorescence development were significantly promoted by adding 3mg/1 IBA and 3mg/1 BA together. Fresh and dry weight and saponin content of plantlet were significantly increased by adding 3mg/1 each of IBA and GA or same amount of IBA and BA. In vitro flowers were appeared at the shoot apex. The regenerated plantlets had the 2 petioles such like field grown 2 yearold ginseng.

人蔘 胚培養에 있어서 花器出現 特性

Chan Moon Chung(鄭燦文),Youl Young Chung(鄭悅永),Jae Seong Jo(曺在星) 한국육종학회 1995 한국육종학회지 Vol.27 No.4

This study was carried out to examine the path way of in vitro flower through embryo culture of Korean ginseng(Panax ginseng C. A. Meyer). Zygotic embryos were cultured on Murashige and Skoog(MS) medium with various concentrations of indolebutyric acid(IBA), benzyladenine(BA) and gibberellic acid(GA). There were two pathway of differentiations from the adventitious buds and shoot primordium in relation to the in vitro flower development. In this case, there are three types of flower emergence : shoot only, flowers only and shoot with flowers. When the amount of in vitro flower differentiation increased, the anther development was poor. The primary culture at 18℃ and the secondary culture at 25℃ under light condition was optimum for the in vitro flower induction.

人蔘 休眠芽培養에 있어 生長調節物質이 休眠打破에 미치는 影響

Chan Moon Chung(鄭燦文),Ki Yeul Nam(南基烈),Yo Tae Kim(金鐃,泰) 한국육종학회 1989 한국육종학회지 Vol.21 No.1

This study was carried out to investigate the possibility of the shortening of generation and mass propagation by in vitro propagation of dormancy bud which is taken out from rhizome of Panax ginseng. There were investigated the effect of MS medium supplemented with GA, NAA, or IBA on dormancy breaking and rooting. Application of GA 30mg/ℓ in MS medium was effect on reducing of dormancy period and expanding of leaf. For rooting and root growth, application of IBA was more effective than that of NAA. Application of IBA 5mg/ℓ alone, or NAA 1mg/ℓ and IBA 5mg/ℓ combination resulted better effect. The rooting of the young bud was possible by treatment of IBA and NAA after dormancy breaking and leaf expansion by GA treatment. Rooting induction of old-aged ginseng was possible, however was so weaker than that of young one. Differentition of root showed vigorous growth of fine root without thickening of primary root.