실크 피브로인을 함유한 비출혈용 복합 지혈제 개발에 대한 연구

문보미 ( Bo Mi Moon ),주형우 ( Hyung Woo Ju ),박현정 ( Hyun Jung Park ),이정민 ( Jung Min Lee ),이옥주 ( Ok Joo Lee ),박찬흠 ( Chan Hum Park ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.2s

Epistaxis is defined as active bleeding arising from the nasal mucosa. It constitutes one of the most common otolaryngology emergencies and can be severe or even fatal. Hemostasis is a process which causes bleeding to stop, meaning to keep blood within a damaged blood vessel which involves differential sequential events. This system in humans has been the most extensively researched. However, lot of research work is going on and lot more needs to be done to overcome the problem associated with this serious issue. This study was conducted to develop a new sponge type of biomaterial to be used for ideal hemostatic agent. The combination of three biomaterials; Silk fibroin (SF), Hyaluronic acid (HA), Gelatin (Gel) and Polyvinyl alcohol (PVA) as proposed hemostatic agent were tested against commercially available hemostatic pad (ChitoClot®). A comparative study of these silk based hemostasis agents designed in combination of SF/HA/PVA and SF/Gel/PVA sponges were tested on injured model of rats. Each sponge to be tested was made same size of (1×1 cm) then applied on top of injury site while applying a pressure of 5 N for 30 seconds. The In vivo experiments were carried by creating a proximal arterial injury in unilateral femoral arteries of 12 anesthetized SD rats. In conclusion, this study suggest a new developed silk fibroin-based sponges as hemostatic materials, which induced durable hemostasis and an increased blood clotting in animal studies. These initial studies explores the loopholes present in the existing ChitoClot® pad compared with that of fabricated silkbased sponges.

조선시대 관문서 관(關)의 기원과 수용 -행이체계를 중심으로-

문보미 ( Bo Mi Moon ) 한국고문서학회 2010 古文書硏究 Vol.37 No.-

도축장 출하돈의 돼지써코바이러스 발생율과 유전형 분석

문보미 ( Bo-mi Moon ),추금숙 ( Keum-sook Chu ),김승채 ( Seung-chai Kim ),김환주 ( Hwan-ju Kim ),김다정 ( Da-jeong Kim ),김원일 ( Won-il Kim ) 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.4

This study was carried out to investigate the genotypic diversity of PCV2 and co-infection of PCV3 in the hilar lymph nodes of 700 randomly-selected slaughter pigs. Fourteen samples per each farm were obtained from 50 farms between February and August in 2022. Of the 50 farms, 44 farms that had been positive for PCV2 by RT-PCR were genotyped. As a result of PCV2 genotyping, positive rate of PCV2 DNA was 62.3% (436/700). Among the PCV2 DNA-positive samples, positive rate of a single PCV2 genotype was 79.1% (345/436), while multiple PCV2 genotypes were only detected in 20.9% (91/436). Of the 436 single infection cases, PCV2d genotype was most prevalent. Positive rates of PCV2 and PCV3 were 53.6% and 26.0% at the sample level, 5.1% and 8.0% at the farm level, respectively. And the co-positive rate of two viruses was 8.7% (61/700) at the sample level, 62.0% (31/50) at the farm level. These results demonstrate that PCV2 prevalence in slaughter pigs is very high and co-infection between different PCV2 genotypes and between PCV2 and PCV3 is relatively common. Therefore, genetic diversity and co-infection between other porcine circoviruses should be consistently monitored in the future.

전북지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석

박미연 ( Mi-yeon Park ),문보미 ( Bo-mi Moon ),강수진 ( Su-jin Gang ),이종하 ( Jong-ha Lee ),박진우 ( Jin-woo Park ),조성우 ( Sung-woo Cho ),허철호 ( Cheol-ho Her ) 한국동물위생학회(구 한국가축위생학회) 2021 韓國家畜衛生學會誌 Vol.44 No.2

Although many swine farms continuously vaccinated to sow to prevent Porcine epidemic diarrhea(PED), PED has occurred annually in swine herds in Jeonbuk province, Korea. In the present study, the small intestine and feces samples from 17 farms where severe watery diarrhea and death of newborn piglets occurred in 2019 were collected, amplified by RT-PCR and determined the complete nucleotide sequences of the spike (S) glycoprotein genes of nine Jeonbuk PEDV isolates. The spike (S) glycoprotein is an important determinant for molecular characterization and genetic relationship of PEDV. These nine complete S gene isolates were compared with other PEDV reference strains to identify the molecular diversity, phylogenetic relationships and antigenicity analysis. 9 field strains share 98.5∼100% homologies with each other at the nucleotide sequence level and 97.3∼100% homologies with each other at the amino acid level. The nine Jeonbuk PEDV isolates were classified into G2b group including a genetic specific signal, S-indels (insertion and deletion of S gene). In addition, comparisons the neutralizing epitopes of S gene between 9 field strains and domestic vaccine strains of Korea mutated 12-15 amino acids with SM-98-1 (G1a group) and mutated 0-3 amino acids with QIAP1401 (G2b group). Therefore, the development of G2b-based live vaccines will have to be expedited to ensure effective prevention of endemic PED in Korea. In addition, we will need to be prepared with periodic updates of preventive vaccines based on the PEDV variants for the re-emergence of a virulent strain.

초음파와 동결/융해에 의한 실크/PVA 하이드로젤의 제조 및 특성 평가

이옥주(Ok Joo Lee),김정호(Jung Ho Kim),주형우(Hyung Woo Ju),문보미(Bo Mi Moon),박현정(Hyun Jung Park),Faheem A Sheikh,박찬흠(Chan Hum Park) 한국고분자학회 2013 폴리머 Vol.37 No.6

실크 피브로인과 poly(vinyl alcohol)(PVA)는 뛰어난 생체적합성과 수용성을 가져 생체의학 분야에서 주목하는 재료이다. 본 연구에서는 실크 피브로인과 PVA를 초음파와 동결/융해 방법으로 드레싱제로서 사용 가능한 하이드로젤을 제조하고자 하였다. 실크와 PVA를 100/0, 75/25, 50/50, 25/75, 0/100 비율로 혼합하였다. 제작한 하이드로젤을 FE-SEM, TGA, FTIR, 압축 강도 측정 등을 통해 물성을 분석하였다. 실크/PVA 하이드로젤은 PVA 함량이 증가할수록 공극 크기와 팽윤도는 감소하였으며, 젤의 강도는 증가하였다. PVA를 첨가함으로써 실크의 기계적 물성이향상되는 것을 확인하였다. 본 연구에서 제조된 실크/PVA 하이드로젤은 드레싱제로서 사용 가능성을 제시하였다. Biomaterials like silk fibroin (SF) and poly(vinyl alcohol) (PVA) have received increasing attention in biomedical applications because of their attractive properties such as hydrophobicity and biocompatibility. In this study, efficient systems consisting of interpenetrating SF/PVA hydrogels were prepared as potential candidate for wound dressing applications. A simple approach consisting of sonication and a freezing-thawing technique was adopted to fabricate the hydrogels. Different blend ratios consisting of SF (100, 75, 50, 25 and 0%) with respect to the weight of PVA were prepared. The produced hydrogels were characterized for physico-chemical investigations using various states of techniques like; FE-SEM, TGA, FTIR and tensile strength. The addition of PVA to SF was proved to be beneficial in terms of reducing the pore size and swelling ratio of hydrogels. The mechanical property of SF had been increased by addition of PVA. These results show that SF/PVA hydrogels may serve as potential candidates for wound dressing application.

PCL/TiO₂ Nanoparticle 3차원 지지체 제조 및 특성 평가

김정호(Jun Ho Kim),이옥주(Ok Joo Lee),Faheem A Sheikh,주형우(Hyung Woo Ju),문보미(Bo Mi Moon),박현정(Hyun Jung Park),박찬흠(Chan Hum Park) 한국고분자학회 2014 폴리머 Vol.38 No.2

Polycaprolactone(PCL)은 생분해성 고분자로 인장강도, 신장률, 충격강도 등의 기계적 물성이 우수하다. TiO2(titanium dioxide) nanoparticle은 친수성으로 밀도가 높고 생체적합성이 우수하다. 본 연구에서는 PCL과 TiO2(titaniumdioxide) nanoparticle을 이용하여 salt-leaching방법으로 3차원 다공성 지지체를 제작하였다. 제작한 지지체를 FESEM,FTIR, TGA, 압축강도 측정 등을 통해 물성을 분석하였다. TiO2 nanoparticle에 의해 물흡수도와 팽윤도는 감소하였으나 압축강도는 증가하였다. CCK-8 assay를 통해 세포의 증식률을 확인한 결과, TiO2 nanoparticle에 의한세포 독성은 없는 것으로 확인되었다. 이러한 연구결과는 PCL/TiO2 nanoparticle 지지체의 생체재료로 사용가능성을 제시하였다. Polycaprolactone (PCL) is a synthetic biodegradable polymer with excellent mechanical properties. TiO2 (tita-nium dioxide) has a hydrophilic, high density and excellent biocompatibility. In this work, we produced three-dimensional porous scaffolds with PCL and TiO2 nanoparticles using a salt-leaching method. Physical properties of the scaffolds were analyzed by FE-SEM, FTIR, TGA and compressive strength. Interestingly, the addition of TiO2 nanoparticles decreased the water absorption and swelling ratio of the porous scaffolds. However, the compressive strength was increased by TiO2. CCK-8 assay, which is generally used for the analysis of cell growth, shows that TiO2 nanoparticles have no cytotoxicity. Taken together, we suggest that the PLC/TiO2-scaffold can be used for biomedical applications.