Kluyveromyces fragilis 의 Inulase 분리 정제

남백희,변시명 ( Baek Hie Nahm,Si Myung Byun ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.2

Extracellular inulase which hydrolyzes β-2:1` linked fructose polymer, inulin, was prepared from Kluyveromyces fragilis in the media containing the Jerusalem artichoke tuber extract. Further purification of the enzyme by Sepharose GB gel filtration followed by DEAE-Sephadex A-50 ion exchange chromatography showed a single protein band on 5% polyacrylamide gel electrophoresis with 232-fold purification. Using the purified enzyme some kinetic parameters of inulase were studied with comparison of invertase activity on sucrose. The enzyme was found to be a glycoprotein and had both inulase and invertase activities, although the general properties varied against substrates in terms of pH, Km, and temperature. The mode of hydrolytic action on inulin by the purified enzyme preparation appeared to be single chain mechanism.

Purification and Characterization of Inulase from Kluyveromyces fragilis

남백희,변시명,Nahm, Baek-Hie,Byun, Si-Myung 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.2

Kluyνeromyces fragilis를 돼지감자 추출액 배지에 배양하여 배지내에 분비된 inulase를 Sepharose 6B겔로 여과한 후 DEAE-Sephadex A-50을 사용하여 효소를 정제 하였다. 이 방법에 의하여 232배 정제하였으며 정제된 효소는 5% polyacrylamide 전기 영동상에서 순수함을 알았다. 순수 분리한 효소를 사용하여 효소의 성질을 조사하였으며 이 효소는 탄수화물을 구성물질로 함유하는 당단백질임을 밝혔다. 효소는 기질에 따라 최적 pH, 최적 온도 및 Km 등이 달랐으나 inulin과 sucrose를 다 분해할 수 있음을 알았다. inulin의 가수분해 양상은 single-chain mechanism 임을 밝혔다. Extracellular inulase which hydrolyzes $\beta$-2:1' linked fructose polymer, inulin, was prepared from Kluyveromyces fragilis in the media containing the Jerusalem artichoke tuber extract. Further purification of the enzyme by Sepharose 6B gel filtration followed by DEAE-Sephadex A-50 ion exchange chromatography showed a single protein band on 5% polyacrylamide gel electrophoresis with 232-fold purification. Using the purified enzyme some kinetic parameters of inulase were studied with comparison of invertase activity on sucrose. The enzyme was found to be a glycoprotein and had both inulase and invertase activities, although the general properties varied against substrates in terms of pH, Km, and temperature. The mode of hydrolytic action on inulin by the purified enzyme preparation appeared to be single chain mechanism.

벼에서의 아밀로즈 생합성 관련 Wx 단백질의 동정 및 분리

남백희(Baek Hie Nahm),김진구(Jin Ku Kim),최해춘(Hae Choon Choi) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6

The Wx protein, known as starch synthase or starch glucosyl transferase (E.C. 2.4.1.11), is responsible for the amylose synthesis. In an effort to explain the mechanism of amylose biosynthesis, the starch synthase known as Wx protein was identified by analyzing the various wx rice mutants with SDS-PAGE of proteins extracted from rice starch. Finally, the 66kDa protein was purified by extracting the starch-bound protein fractions followed by Suprose 12 gel filtration chromatography.

AceDB , the Organism - Specific Genome Database

남백희(Baek Hie Nahm),(Tae Ho Lee),(Byong Iyol Lee),(Moo Young Eun) 생화학분자생물학회 1997 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

Detection of DNA Rearrangement in Rice Using a Cosmid Library

문은표,남백희,Mun, Eun-Pyo,Nahm, Baek-Hie The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.4

Previously we reported the migration and rearrangement of a chloroplast gene cluster into mitochondria. The exact genomic locations of the clusters, modes of the gene rearrangement and mechanisms of the interorganellar migration of the clusters have yet to be understood. The detailed analysis needs to include a larger region of DNA surrounding each cluster. To study DNA rearrangement and migration in more detail a cosmid library was constructed using the total rice genomic DNA including nuclear, chloroplast and mitochondrial DNA. From this cosmid library, a sub-library was obtained by selecting the clones hybridized to various regions of chloroplast DNA. According to the hybridization pattern 136 clones from the sub-library were classified into 29 groups. Detailed analysis of these clones revealed that in addition to authentic chloroplast DNA, the clones contain its homologs resulted from rearrangement and mutation. We analyzed two clones in detail, which contain different rp12 homologs resulted from rearrangement and/or migration, respectively.

벼 배발생 세포의 특성과 배발생 관련 유전자의 분리 - 배발생 세포에 관련된 동위효소 (同位酵素) 특성 -

정병균(Byung Kyun Jung),백윤웅(Yun Woong Paek),고경민(Kyeong Min Ko),남백희(Baek Hie Nahm),황백(Baik Hwang) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.1

N/A

쌀 저장 단백질 프롤라민 유전자 암호 분석

이태호(Tae Ho Lee),김주곤(Ju Kon Kim),남백희(Baek Hie Nahm) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6

To characterize the prolamines in rice cultivars, the complete coding sequences of 17 prolamine genes from the database were analyzed. According to the phylogenic analysis of the sequences, these genes could be classified into 4 groups, Group Ⅰ to Ⅳ. The multiple alignment of the deduced amino acid sequences revealed that the four groups differ from one another in chain length caused by deletion of short internal amino acids or carboxyl terminal fragments. Each group was also found to have different amino acid composition with 1, 4, 10 and 30% of sulfur containing amino acids (methionine and cysteine) in Group Ⅰ to Ⅳ prolamines, respectively. Also the isoelectric points of these groups showed the different values of 9.2, 8.2, 6.7 and 7.4. Finally, from the analysis of colon usage pattern of prolamine genes, the colon usage for arginine, serine, threonine, isoleucine, asparagine, aspartic acid, glutamic acid and cysteine were highly biased. In the analysis of the colon usage pattern, the relation of the fraction of G/C ending colons to effective colon numbers suggests the different translational efficiency in the expression of the prolamine multigenes.

쌀 저장 단백질 글루텔린 유전자 암호 분석

신윤철(Yun Cheol Shin),김주곤(Ju Kon Kim),남백희(Baek Hie Nahm) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6

To characterize glutelins, the most abundant storage protein in rice, 13 complete coding sequences of glutelin genes from the database were analyzed. According to the phylogenic analysis, these genes could be classified into 5 groups, Group I to V. The degrees of homology were calculated to be in the range of 90 to 60%, but the patterns of hydrophobicity were similar in all the groups. Also, each group was found to have similar amino acid composition with variations in lysine content from 2.5 to 3.6% due to the point mutation of arginine to lysine. The isoelectric points of mature proteins and their basic chains of all the groups showed the value of about 9.0 and 10.0, respectively, while the isoelectric points of acidic chains in these groups showed the distinct value of 6.6, 6.7, 7.2, 8.4 and 7.9. The plot of the fraction of G+C at synonimous site in codons (GC3s) against effective codon numbers suggest no major difference in translational efficiency in the expression of glutelin multigenes.

Physicochemical and structural characteristics of grain associated with palatability in japonica rice

Hae Chune Choi(崔海椿),Ha Cheol Hong(洪夏鐵),Baek Hie Nahm(南佰熙) 한국육종학회 1997 한국육종학회지 Vol.29 No.1

To elucidate the major components of rice quality associated with the palatability in japonica, twelve japonica rice cultivars were examined for several physicochemical properties, endosperm structure of rice grain and physical characteristics or sensory palatability of cooked rice. The rice materials used showed narrow varietal variation in grain size and shape, alkali digestibility, gel consistency and amylose and protein contents, but considerable difference in appearance and texture of cooked rice in two years. Significant variations in varieties (V), harvested years (Y), and V x Y interaction were recognized in most of the physicochemical properties except alkali digestibility, volume expansion rate and texturogram of cooked rice. There was relatively larger coefficients of variation for both varieties and years in Mg/K ratio, iodine blue value of extracted solids and amounts of extracted solid matter during cooking, breakdown and setback viscosity, and short chain ratio of amylopectin. The physicochemical properties significantly associated with glossiness or global palatability score of cooked rice were the peak, hot paste and consistency viscosities of viscogram with year difference. The high-quality rice variety “Ilpumbyeo” showed less portion of amylose on the outer layer of milled rice grain and less and slower change in iodine blue value of extracted paste during twenty minutes of boiling. This highly palatable rice also exhibited very fine net structure in outer layer and fine-spongy and well-swollen shape of gelatinized starch granules in inner layer and core of cooked rice kernel compared with the poor palatable rice. Global sensory score of cooked rice could be estimated by multiple linear regression formula, deduced from relationship between rice quality components mentioned above and eating quality of cooked rice, with high probability of determination in two years. Inconsistent distribution of tested japonica rices over years on the plane of two integrated quality components indicated that there were some different high-quality rice types and those environmental response on grain quality was greatly different.

벼 microarray를 이용한 유전자발현 profiling 연구동향

윤웅한,김연기,김창국,한장호,이태호,김동헌,이강섭,박수철,남백희,Yoon, Ung-Han,Kim, Yeon-Ki,Kim, Chang-Kug,Hahn, Jang-Ho,Kim, Dong-Hern,Lee, Tae-Ho,Lee, Gang-Seob,Park, Soo-Chul,Nahm, Baek-Hie 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2

As the International Rice Genome Sequencing Project (IRGSP) was completed in 2005 and opened to the public, many countries are making a lot of investments in researches on the utilization of sequence information along with system development. Also, the necessity of the functional genomics researches using microarray is increased currently to secure unique genes related with major agricultural traits and analyze metabolic pathways. Microrarray enables efficient analysis of large scale gene expression and related transcription regulation. This review aims to introduce available microarrays made based on rice genome information and current status of gene expression analysis using these microarrays integrated with the databases available to the public. Also, we introduce the researches on the large scale functional analysis of genes related with useful traits and genetic networks. Understanding of the mechanism related with mutual interaction between proteins with co-expression among rice genes can be utilized in the researches for improving major agricultural traits. The direct and indirect interactions of various genes would provide new functionality of rice. The recent results of the various expression profiling analysis in rice will promote functional genomic researches in plants including rice and provide the scientists involved in applications researches with wide variety of expression informations.