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조석우,민태진,이갑득 동국대학교 자연과학연구소 1987 자연과학연구 논문집 Vol.7 No.-
표고버선의 미토큰드리아는 설탕밀도 선형기울기 원심분리법으로 정제하여, 광감음성 mitochondrial ATPase의 유기물 효과, 광증감제 효과 및 파장변화에 따른 K 이온의 유입효과를 실험하였다. 1.이 효소는 10mmol dithiothreitol, 및0.1mmol quinacrine에 의하여 각각 139% 및 128%의 활성도를 증가시켰다. 2.100ug의 oligomycin과 1mmol phlorizin은 이효소의 활성을 각각 48% 및 45%억제 시켰다. 3.광증감제인 0.1mmol phenazine methosulfate는 이 효소의 활성도를 36% 촉진시켰다. 4.K이온 유입 효과의 최적 파장은 690nm였고, 이때의 최적 pH 및 최적 온도는 각각 7.2 및 55%였다. Effects of organic compound, photosentizer and K in influx on the light-induced ATPase of mitochondria in L. deodes purified by linear sucrose density gradient centrifugation were studied. The motochondrial ATPase activity was investigated by various wavelength illumination at dark state. The mitochlndrial ATPase was activated 139% and 128% by 10mmol dithiotheitol and 0.1mmol quinacrine, respectively. This enzyme also was activated 36% by 0.1mmol phenazine, methoulfate as photosensitizer. But 100ug oligomycin and 1mmol phlorizin ingibited activited activity of enzyme to 48% and 45%, respectively. Its optimum wavelength was 690nm on the effect of K ion influx, its optimum pH and temperature were found to be 7.2 and 55℃.
P_(b)^(++) ion選擇性 膜電極에 依한 K₄Fe(CN)_(6)의 定量 및 C_(O)^(++) ion의 間接定量法
李甲得 慶北專門大學 (영주경상전문대학) 1983 慶北專門大學 論文集 Vol.4 No.-
K_(4)Fe(CN)_(6)의 定量法은 中量法, 比色法, 銀ion 選擇性 膜電極에 依한 定量法等이 알려져 있으나 銀ion 選擇性 膜電極에 依한 定量法은 아직 알려져 있지 않다. Horiba製 銀ion 選擇性 膜電極을 指示電極으로 Calomel電極을 參照電極으로 하여 pH4∼5.5範圍內에서 0.1M과 O.OlM - Pb(NO_(3))_(2),標準溶液을 電位差 滴定하였던 바 當量點과 잘 一致되는 銳敏한 變曲點을 얻었다. 이 結果를 써서 Fe(CN)_(6)^(4) ion과 難溶性 沈澱을 形成하여 金屬ion의 間接定量에 利用코저 Cobaltion 定量에 ·定過量의 Fe(CN)_(6)溶液을 加하고 침전을 KNO_(3), 電解質存在下에 違心分離한 沪液에 Methanol을 添加하고 上記 指示電極으로 Pb標準溶液을 電位差 滴定하여 만족스러운 結果를 얻었다.
연어 ( Salmo Salar ) 알중의 Carboxypeptidase A의 분리정제 및 그 성질에 관한 연구
이갑득,민태진,김준행 생화학분자생물학회 1991 BMB Reports Vol.19 No.3
The enzymic properties of carboxypeptidase A in the eggs of salmon (Salmo solar) were investigated by purification with CM-cellulose, ammonium sulfate fractionation, DEAF-cellulose, and sephadex G-75 gel filtration. The fraction A showed its activity toward substrate such as bovine hemoglobin and hippuryl-L-phenylalanine. The molecular weight of this enzyme was determined to be 44,000 daltons by SDS-PAG electrophoresis and the enzyme was monomeric protein composed of 18 different amino acids. Optimum pH was 4.5, optimum temperature was 60℃, and the enzyme was stable at pH 2.5-5.5 and 20°-60℃. This enzyme showed substrate specificity hydrolyzing the peptide bond of glycyl-L-phenylalanine. Its K_m value was 0.29 mmol and the enzyme was inhibited by Zn^(2+), Fe^(3+), and L-cysteine. The L-cysteine was found to be competitive inhibitor, and its K_i value was determined to be 1.2 mmol by Dixon plot.
이갑득 한국생명과학회 2000 생명과학회지 Vol.10 No.5
The action spectrum for light-induced growth of fruit body in Pleurotus ostreatus has been studied by irradiation at various wavelengths. Effective wavelengths were distributed from near ultraviolet to blue region of spectrum. The most effect of light was observed in the region between 340 to 520 nanometers. The growth weigth obtained from which has been irradiated 144 hours in the region of 340-520 nm increases 74%, on the contrary the observed growth weight has been observed in the longer wavelengths than 620 nm. The pileus size was promoted 30$\times$32% in the region of 340-500 nm, and retarded 22$\times$19% in the dark. the strip length was rearded 12% in the region of 340-500 nm, and promoted 38% in the dark. The color thickness for pileus is getting light in longer wavelengths.
李甲得 慶北專門大學 (영주경상전문대학) 1984 慶北專門大學 論文集 Vol.5 No.-
몇개의 製빵工場 및 製紙에서의 廢水汚染의 주요 원인은 물엿 食品製造工程 및 使用機械數와 세척제 使用으로 起因된 것인데 여기에서 나오는 原廢水와 處理後 廢水를 비교 검토한 결과는 아래와 같다. 1) 廢水處理에 있어 첫째 問題가 PH인데 Butter solution을 써서 PH6으로하여 P.A.C.를 투입하였든바 Fig3, Fig4, 와 같은 淨水효과를 얻었다. 2) 두번째 問題가 C.O.D인데 C.O.D를 적게하기 위해서 P.A.C를 淨水藥劑로 淨水한 結果 TABLE-V에서 보는 바와 같이 정수효율면에서 C.O.D 및 濁度에서 훌륭한 淨水 能力을 나타내었다. 3) 工業用水規格의 數直와 비교해 볼때 C.O.D. 및 獨度 PH의 項目에서 回水用水로 再使用할 수 있다고 思料됨. 4) 이상의 관점으로 볼때 本實驗에 제공된 試料水와 유사성이 있는 食品工場의 廢水 및 製紙工場의 廢水는 淨水藥劑處理로서 回水用水로 使用可能한 工業用水로 淨水處理할 수 있다고 思料된다.
鹽基性 黃酸第二鐵의 合成과 組成分析 및 淨水能力과P.A.A.첨과효과에 關한 硏究
李甲得 慶北專門大學 (영주경상전문대학) 1981 慶北專門大學 論文集 Vol.3 No.-
In order to curtail the additional use of alkali, an auxiliary agent of water-purifying reagent in the iron series, an adequate basic sulfate synthesis and an analysis of component elements in the formative materials in accordance with the synthetic formulations were givn.The resulted study of the influencing power from under various conditions the synthesized water-purifying reagent revealed as follows: 1. Manufactured No. 1 is highly basic, but because of easy occurrence of hydrolysis it is of little practical use. 2. Considering the additive quantity of water-purifying reagent and remaing turbid degree of lime water, it show that manufactured No.2 is the best for manufactured goods. 3. In general, pH-8 are easy to cause floe formations, and in view of lower degree of the remaining turbid it is effective for the weak acidic waster-water treatment. 4. Although salt does not have sufficient effect on the water-purifying reagent, the remaining turbid degree tends to increase in accordance with the additive amount. 5. In the series of experiments the addition of P.A.A increasingly takes effect on water purification.