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Glucose와 Phosphate가 제거된 M-TALP 배지에서의 난구세포 공배양에 의한 임신율 향상에 관한 연구
정범식,장우현,이문희,김지연,방지호,김규현,서태광,Chung, B.S.,Chang, W.H.,Lee, M.H.,Kim, J.Y.,Bang, J.H.,Kim, K.H.,Suh, T.K. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1
The beneficial effect of glucose and phosphate ions in culture medium on the development of human embryos in vitro has not been fully elucidated. The purpose of this study was to evaluate the influence of fertilization and culture of embryos in glucose/phosphate-free m-TALP medium on pregnancy rates in IVF-ET program. The patients in 244 IVF-ET cycles received GnRH agonist + HMG regimens. A does of 10,000 IU HCG was administered when two or more dominent follicles reached 18mm in diameter. Thirty-six hours after HCG, oocytes were recovered transvaginally using ultrasound guidance. Aspirated oocytes were matured for 4 to 6 h in TCM-199 supplemented with 10% follicular fluid (FF). Insemination was carried out with 50,000 motile spermatozoa in TCM-199 + 10% FF or m-TALP + 5% FF + 5% fetal cord serum (FCS) according to experimental design. After 6 h, oocytes were washed 3 to 4 times and cultured in each fresh medium. After 20 h, oocytes were freed from cumulus/corona cells and examined for the presence of pronuclei. Fertilized oocytes were transferred into each co-culture drops and cultured for further incubation. On day 3, embryo transfer was performed with grade 1 and 2 embryos. Monolayers for co-culture of embryos were prepared by plating $1{\times}10^5$ cumulus cells/ml in 10ul drop of TCM-199 + 10% FF or m-TALP + 5% FF + 5% FCS media 24 h prior to the onset of co-culture. Development to 4 to 16 cell stage was observed at 70x magnification following two days of incubation. Pregnancy was confirmed by detecting increasing serum ${\beta}$-hCG concentrations for 11 days following embryo transfer. Data were analyzed by ${\chi}^2$-test. Oocytes from 244 IVF-ET cycles were randomized. The number of cycles and mean age of patients were 97 and 147, 31.3 yrs and 31.2 yrs for TCM-199 (control) and m-TALP groups, respectively. The mean number of retrieved oocytes/cycle, fertilization rates, number of embryos transferred/ET and pregnancy rates were 11.1 and 10.3, 65.1% and 67.3%, 4.1 and 4.7, 28.9% and 43.8% for TCM-199 and m-TALP groups, respectively. Differences in the pregnancy rates were found between control and m-TALP groups (p<0.05). The pregnancy rate of patients divided according to maternal age groups of ${\leq}30$, 31-35, $36{\leq}$ were 44.4% and 49.0%, 26.1% and 41.3%, 29.2% and 41.2% for control and m-TALP groups, respectively. These data indicate that culture of human embryos in glucose/phosphate-free m-TALP medium improves pregnancy rates.
수도에 대한 Ethylene의 생리작용에 관한 연구 제4보 질소반응과 Ethylene생성의 품종간 차이
이문희,이종훈,태전보부,Lee, M.H.,Lee, J.H.,Ota, Yasuo 한국작물학회 1983 한국작물학회지 Vol.28 No.1
Rice plant was tested in the phytotron to know the varietal difference of ethylene production in different nitrogen levels. The high nitrogen concentrated varieties among tested varieties produced a large amount of ethylene. But high nitrogen concentrated leaf in the one variety produced less amount of ethylene. The varieties which produced a large amount of ethylene was remarkably increased ethylene in no nitrogen plot$(N_0)$ compare to standard level$(N_1)$. In contrast low ethylene producing varieties was remarkably decreased in double nitrogen level$(N_2)$.
수도에 대한 시비방법이 질소효율 및 잡초군락에 미치는 영향
이문희,Lee, M.H.,Datta, S.K. De 한국작물학회 1976 한국작물학회지 Vol.21 No.2
This experiment was conducted to find out how to increase efficiency of fertilizer nitrogen and how to change the weed population with different methods of nitrogen application. Mudball deep placement, at 10-12cm soil depth, produced significantly the highest grain yield within the application methods with same amount of nitrogen (60kg N/ha). It produced also same grain yield with conventional application methods, timely split application method, with 90kg N/ha. Basal application of nitrogen increased weed population and it showed higher dry weight of weed than top dressing methods at early growth stage of rice.