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Most of the molecular linkage maps in rice were constructed using segregating populations derived from crosses between parents with a wide genetic distance, such as japonica and indica rices, because of the high polymorphic profiles in them. However, the molecular markers in genetic maps in the populations of japonica/japonica or indica/indica are more useful in practice since these markers can be utilized directly in the concurrent breeding programs. This study aims to develop a genetic map using a population derived from early heading japonica cultivar Olbyeo1 and late heading cultivar Ilpumbyeo. Plant height and heading dates were checked in 300 F2 plants, from which randomly selected sixty plants were analyzed with DNA markers. One hundred and twenty nine polymorphic markers were detected in the segregating population and fifty markers were located in seven linkage groups. Total genetic distance of the seven linkage group was 795.5 cM with an average 17 cM distance between markers. Two QTL for heading days were detected in the linkage group 4 whose individual LOD values were 4.06 and 6.69, respectively. Genetic synteny of the seven linkage groups in the present study is being surveyed with the established rice linkage maps with more molecular markers.
This study was conducted to analyze quantitative trait loci (QTL) of alkali digestion value (ADV) and amylose content. The recombinant inbred lines (RILs) were developed by single seed descent (SSD) method of progenies derived from the cross between Japonical/Tongil hybrid cultivar, Nonganbyeo, and indica elite line, BG276. The 272 individual plants of F_7 generation were used for DNA analysis by simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) techniques to construct the molecular linkage map on which the loci of the QTLs governing the characters were determined. The total length of the 12 linkage groups spaned to 1,234 cM and its average length between the markers was 9.4 cM. A total of 7 significant QTL governing 2 characters was detected with the number variation of 3 to 4 QTL for each character. For the characters controlling the grain quality and cooking behavior, a fatal of 4 QTL for ADV was located in chromosome 3, 7, 8, and 11, and 3 QTL for amylose content of grains in chromosome 1, 6, and 11. The phenotypic variation of respective characters explained by these QTL markers were 70% for ADV and 79.5% for amylose content of grains.
To investigate the effects of harvesting times on physicochemical and milling properties of rice, milling tests were performed applying laboratory milling systems. Milling and head rice yields were highest in rice harvested at the 45 days after heading at 76.49 and 94.43%, respectively. Milled rice grain harvested 45 days after heading showed the highest minimum viscosity, as shown in the amylogram curves, as well as the lowest consistency viscosity. Protein and amylose contents were highest in rice harvested at 35 days after heading, whereas the instrumental taste score was highest 45 days after heading.