갑상선 수질암의 세침흡인 세포학적 소견 - 1 증례 보고 -

주영채,황태숙,Chu, Young-Chae,Hwang, Tae-Sook 대한세포병리학회 1991 대한세포병리학회지 Vol.2 No.2

A 33-year-old woman who was diagnosed as medullary carcinoma by fine needle aspiration of thyroid mass is presented. The smear revealed dispersed pattern and small clusters of cells without follicular or papillary structures. The nuclei were round, oval or spindle shaped and eccentrically located. The cytoplasm was abundant and showed red-stained cytoplasmic granulation and tail-like projection with indistinct border. Clumps of amorphous, light-green material were intermingled with tumor cells.

Helicobacter pylori 연관 철분 결핍성 빈혈과 H. pylori pfr 유전자 다형성과의 관련성

이지은,최연호,황태숙,Lee, Ji-Eun,Choe, Yon-Ho,Hwang, Tae-Sook 대한소아소화기영양학회 2001 Pediatric gastroenterology, hepatology & nutrition Vol.4 No.1

목 적: H. pylori 감염은 특히 사춘기에서 철분결핍 빈혈의 유발에 관여하는 것으로 여겨진다. H. pylori의 ferritin 단백질인 Pfr은 진핵생물과 원핵생물의 ferritin과 동일하다. 본 연구는 철분 결핍 빈혈이 있거나 혹은 없는 H. pylori 양성 전정부위염환자의 위 생검 표본에서 H. pylori pfr 유전자를 비교 분석하고자 하였다. 방 법: 총 26명의 H. pylori 양성 전정부위염 환자(10~18세)들을 철분 결핍 빈혈의 유무에 따라 두 군으로 나뉘었다. 16명의 환자가 혈액학적 검사를 통해 철분 결핍 빈혈이 있는 것으로 밝혀졌고 그들 중 2명이 십이지장 궤양이 있었다. 다른 10명은 정상적인 혈액학적 검사 소견을 보였다. 각각의 위 생검 표본에서 DNA 분리가 이루어졌다. 2개의 시발체 세트를 사용해서 pfr 유전자 암호의 PCR증폭이 행해졌고 pfr 부위인 50 1bp는 2개의 PCR산물을 연결하여 완성하였다. nucleotide와 단백질서열이 한국의 H. pylori 균주와 Genbank에서 구한 NCTC 11638, 26695, J99 균주의 pfr 부위 사이에서 비교되었다. 또한 철분 결핍 빈혈 양성인 군과 음성인 군 사이의 pfr 부위에 대한 서열의 비교가 행해졌다. 결 과: pfr 유전자의 암호 부위를 완전히 분석한 결과 3곳에서 다형성이 발견되었다. Ser39Ala 돌연변이는 100% (26/26)에서 발견되었고 Gly111Asn은 26.9% (7/26), Gly82Ser은 11.5% (3/26)였다. 철분결핍 빈혈이 양성인 군과 음성인 군간의 pfr 부위의 다형성은 의미 있는 차이가 없었다. 결 론: pfr 유전자의 다형성은 철분 결핍 빈혈과 같은 임상표현형과 관련이 없었다. H. pylori 감염이 철분 결핍 빈혈을 유발한다는 기전을 명료하게 밝히기 위해 숙주측면의 연구나 혹은 다른 복합적인자를 고려한 연구가 향후 필요할 것으로 보인다. Purpose: H. pylori infection is thought to contribute to iron-deficiency anemia, especially during puberty. The ferritin protein Pfr of H. pylori is homologous to eukaryotic and prokaryotic ferritins. The purpose of this study was to analyze the H. pylori pfr status in gastric biopsy specimens according to clinical data, including antral gastritis with or without iron-deficiency anemia. Methods: A total of 26 H. pylori-positive patients aged from ten to 18 years were categorized into subgroups based on the presence or absence of iron-deficiency anemia. All of them had antral gastritis. Sixteen patients were proved to have iron-deficiency anemia by hematological study, two of which had a duodenal ulcer. The other ten patients showed normal hematological findings. DNA isolation was performed from each of the gastric biopsy specimens. PCR amplification of the pfr gene coding was done using two sets of primers. The pfr region, 501 bp, was generated by linking the sequences of the two PCR products. The nucleotide and protein sequences were compared between the pfr regions from Korean H. pylori strains and the NCTC 11638, 26695, and J99 strain, which were obtained from the Genbank. Sequence comparisons were also performed for the pfr regions between the iron-deficiency anemia (+) and (-) groups. Results: Analysis of the complete coding region of pfr gene revealed three sites of mutation. The Ser39Ala mutation was found in 100% (26/26), Gly111Asn in 26.9% (7/26), and Gly82Ser in 11.5% (3/26). There were no significant differences in the mutations of the pfr regions between the iron deficiency anemia (+) and (-) groups. Conclusion: The mutation in the pfr gene did not relate with the clinical phenotype, iron deficiency anemia. Further studies are needed on the aspects of host side or other complex factors to elucidate anemia. Further studies are needed on the aspects of host side or other complex factors to elucidate the mechanisms by which the H. pylori infection might lead to iron deficiency anemia.

세침흡인된 갑상선의 여포상 선종과 여포상 암종의 형태계측학적 연구

주영채,차희정,민수기,김준미,황태숙,Chu, Young-Chae,Cha, Hee-Jung,Min, Soo-Kee,Kim, Joon-Mee,Hwang, Tae-Sook 대한세포병리학회 1998 대한세포병리학회지 Vol.9 No.1

Fine needle aspiration cytology of "cold" nodules of the thyroid has proved to be of great value in their preoperative diagnosis. Most types of thyroid tumors are readily recognizable from characteristic cellular patterns in the smears of needle aspirates. But follicular neoplasms present some problems because the cytomorphology of the adenomas frequently is same as in carcinoma. For differentiation of benign from malignant follicular neoplasms of the thyroid we tested the usefulness of two objective parameters - nuclear area and perimeter - by morphometry. This study was made on fine needle aspirates from 30 cases with cytologic diagnosis of follicular neoplasm of thyroid. The histologic classification was follicular adenoma in 22 cases and follicular carcinoma in 8 cases. As a reference group we used seven caes with nodular hyperplasia. The smears of aspirates were stained by Papanicolaou method. On each slide 200 randomly selected cells with intact nuclei were measured. The mean value of nuclear area are $25.32{\pm}5.50{\mu}m^2,\;34.08{\pm}7.50{\mu}m^2\;and\;39.97{\pm}6.63{\mu}m^2$ in nodular hyperplasia, follicular adenoma, and follicular carcinoma, respectively. The mean value of perimeter are $19.48{\pm}2.26{\mu}m,\;22.95{\pm}2.65{\mu}m\;and\;24.78{\pm}2.23{\mu}m$ in nodular hyperplasia, follicular adenoma and follicular carcinoma, respectively. The mean nuclear areas and perimeters of cells from follicular adenoma were significantly larger than those from nodular hyperplasia (p<0.05). The mean nuclear areas and perimeters of cells from follicular carcinoma were larger than those from follicular adenoma but the differences are not significant statistically(p>0.05). Therefore, morphometric assessment alone is inadequate to predict malignancy in thyroid aspirates.

Helicobacter pylori vacA 대립유전자의 Mosaicism과 Signal Sequence의 한국고유 시발체

안연화,김흥렬,이지은,황태숙,최연호,Ahn, Yeon-Hwa,Kim, Heung-Ryel,Lee, Ji-Eun,Hwang, Tae-Sook,Choe, Yon-Ho 대한소아소화기영양학회 2001 Pediatric gastroenterology, hepatology & nutrition Vol.4 No.2

목적: H. pylori의 vacA 대립유전자는 종족과 지역에 따라 다양하게 나타나고 있다. 저자들은 H. pylori에 감염된 소아의 위생검조직에서 vacA 대립 유전자에 대한 중합효소연쇄반응과 유전자염기분석을 실시하여 한국의 signal sequence와 mid-region의 다형성 및 고유의 시발체를 연구하고자 하였다. 방법: 상부 위내시경을 시행한 후 H. pylori 감염으로 진단된 10~18세 50명의 환아를 대상으로 위생검조직을 이용하여 vacA 대립유전자에 대한 PCR과 DNA 분석을 실시하였다. 이들 결과와 다른 나라의 vacA 대립유전자를 비교분석하였고 우리나라의 고유한 염기배열을 갖는 시발체를 제작하였다. 결과: 1) 서구의 시발체를 사용한 50명 중 30명(60%)에서 모두 s1이 검출되었고 이중 s1a가 14명, s1c 15명, s1a/s1c hybrid가 한 명이었으며 s1b는 발견되지 않았다. s1c/m1이 가장 많은 형이었다. 2) 우리 나라에 공통으로 발견되는 염기변이가 s1a에서는 GGGAGCGTTR, s1c는 GGGGYTATTG 이었으며 이들을 이용하여 새로운 시발체를 고안하였다(VASK-F, VASK-R, S1AK-F, S1CK-F). 새로이 제작된 시발체로 처음의 50개 조직을 재검한 결과 50개 모두에서 s region이 양성이었다. 결론: 우리 나라의 주된 vacA 대립유전자형조합은 s1c/m1이었고, vacA signal sequence의 한국 고유의 시발체를 만들었음을 보고하는 바이다. Purpose: Helicobacter pylori has been known to have diverse vacA allelic types. The purpose of the study was to identify vacA diversity in Korea and design new primers for signal sequence alleles indigenous to Korea. Methods: Fifty antral biopsy specimens, which had been proven to be H. pylori-positive, were examined for vacA status; signal sequence and mid-region. After PCR amplification and DNA sequencing, vacA alleles of Korean H. pylori strains were compared with those from other countries. Results: Among Korean H. pylori strains vacA alleles with all combinations of signal sequence and mid-region were found, with the exception of s1b or s2. vacA genotype s1c/m1 was predominant in Korea. We found that GGGAGCGTTR in s1a and GGGGYTATTG in s1c were the indigenous sequences to Korea and constructed the new Korean specific primers for the vacA signal sequence; VASK-F, VASK-R, S1AK-F, and S1CK-F. Conclusion: This study showed that s1c/m1 is the predominant type of vacA allele in Korea. We designed new primers for the vacA signal sequence.

포상연부육종의 세침흡인 세포학적 소견 - 1예 보고 -

한혜승,박인서,한지영,김준미,김영배,황태숙,주영채,Han, Hye-Seung,Park, In-Seo,Han, Jee-Young,Kim, Joon-Mee,Kim, Young-Bae,Hwang, Tae-Sook,Chu, Young-Chae 대한세포병리학회 2000 대한세포병리학회지 Vol.11 No.2

Alveolar soft part sarcoma is a rare soft tissue tumor. Few cases on fine needle aspiration cytology have been reported in the literature. We experienced a case of recurrent alveolar soft part sarcoma of the right thigh diagnosed by fine needle aspiration cytology in a 47-year-old man. Cytologic findings showed single cells and clusters associated with thin wailed vasculature in a distinct pseudo-alveolar pattern. The tumor cells exhibited round or ovoid abundant granular cytoplasm and large pleomorphic nuclei with prominent central nucleoli.

한국인 위암에서 점액조직화학법에 의한 표현형 발현양상

김경국(Kyung Kook Kim),최선근(Sun Keun Choi),오성태(Sung Tae Oh),황태숙(Tae Sook Hwang),김영배(Young Bae Kim),김진복(Jin-Pok Kim),지제근(Che Keun Chi) 대한외과학회 1994 Annals of Surgical Treatment and Research(ASRT) Vol.47 No.2

TGF-β 유전자를 포함하는 세포생산 및 가토내 생존

이관희(Kwan Hee Lee),고석면(Suk Myun Ko),황태숙(Tae Sook Hwang),이준규(Jun Kyu Lee),오인석(In Suk Oh),이정윤(Joung Yoon Lee),김성진(Seong-Jin Kim) 대한정형외과학회 1999 대한정형외과학회지 Vol.34 No.1

녹각교가 난소절제 흰쥐에서 골수기질 간세포의 증식 및 분화에 미치는 영향

원영준 ( Young Jun Won ),안혜은 ( Hye Eun Ahn ),문경호 ( Kyoung Ho Moon ),민병현 ( Byung Huyn Min ),황태숙 ( Tae Sook Hwang ),서창국 ( Chang Kook Suh ),박소라 ( So Ra Park ) 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2

Herbal medicines have been claimed to have beneficial effects for the fracture healing and bone formation. Extracts of antler and old antler were known to have effects on the immune modulation, lowering of cholesterol, correction of anemia, detoxification of hepatic injuries, and the growth. However, beneficial effects on the bone metabolism were not determined. In order to investigate the effects of bone metabolism, extracts of old antler were used for in vivo and in vitro study. Eight rats (2-month-old female Sprague-Dawley rats) were placed on Chow diet and served as control (C), and eight rats were got Chow diet with old antler (C+OA) for 12 weeks. Eight rats were done ovariectomy for 4 weeks and had Chow diet (OVX), and another eight ovariectomized rats had Chow diet with old antler (OVX+OA). Sixteen rats were fed Chow diet with 0.4g/kg old antler daily. After 12 weeks, all experimental rats were sacrified for removing the bone marrow stromal stem cells. Bone marrow stromal stem cells rats were cultured and resuspend α-MEM containing 10 % heat inactivated fetal bovine serum (FBS) and antibiotics. Total colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphate stained CFU-Fs cells were counted at 12 days after cell culture. In addition, mineralized nodule was also measured by light microscope at 21 days after cell culture. Total CFU-Fs colony counts were not significant difference among the study groups. Alkaline phosphatase positive colonies of ovariectomized rats with treating of extracts of old antler were significantly increase than those of ovariectomized with Chow diet. The amounts of mineralized nodule of OVX+OA group were significantly increase than those of OVX group. In conclusion, osteogenic differentiation of mesenchymal stem cells were enhanced after treated with extracts of old antler on ovariectomized rats.

대장 종양에서의 Sonic Hedgehog 단백의 발현

한혜승 ( Hye Seung Han ),이선영 ( Sun Young Lee ),성무경 ( Moo Kyung Seong ),김정환 ( Jeong Hwan Kim ),성인경 ( In Kyung Sung ),박형석 ( Hyung Seok Park ),진춘조 ( Choon Jo Jin ),황태숙 ( Tae Sook Hwang ) 대한장연구학회 2008 Intestinal Research Vol.6 No.1

Background/Aims: Dysregulation of the hedgehog pathway has been implicated in regeneration and carcinogenesis, leading to the expression of the sonic hedgehog (Shh) protein in gastrointestinal neoplasms. The expression of Shh in colon neoplasms and paired normal colonic mucosa was therefore investigated. Methods: Forty-four colon cancers and 73 colon adenomas that were removed by surgical colectomy or colon polypectomy between August 2005 and August 2006 were included. Colorectal neoplasms and the adjacent normal colon tissue were examined by immunohistochemistry using rabbit polyclonal Shh antibody. Results: Expression of Shh was up-regulated in adenomas and adenocarcinomas of the colon compared to normal colon tissues (p<0.001). The degree of Shh expression was not associated with the size, shape, or, location of the tumor, or as the age and gender of the patient. In normal colonic epithelium, Shh was expressed at the apex of the crypts and in a few basally-located cells. Conclusions: Higher levels of Shh expression in colonic adenoma and adenocarcinoma suggest that Shh is required during epithelial proliferation in the colon. Hedgehog signaling is likely to be associated with early tumorigenesis in colonic neoplasms. (Intest Res 2008;6:19-24)

1)대장직장암에서 임상조직학적 소견과 DNA ploidy와의 상관관계

이석호(Suk Ho Lee),김헌정(Hun Jung Kim),김우철(Woo Chul Kim),조영갑(Young Kap Cho),노준규(John J K Loh),우제홍(Ze Hong Woo),황태숙(Tae Sook Hwang) 대한방사선종양학회 2000 Radiation Oncology Journal Vol.18 No.3

Purpose : DNA ploidy pattern was shown to correlate with several clinicohistologic findings in several tumors. Aim of this study was to evaluate the correlation of the clinicohistologic findings in colorectal cancer and the failure pattern in rectosigmoid cancer with DNA ploidy. Materials and Methods : DNA flow cytometry using the Hedley methods on paraffin embedded specimen from 117 patients with colorectal cancers after curative resection was performed. We tried to find the correlation between DNA ploidy and various clinicohistologic findings. And then the correlation DNA ploidy and the failure pattern in 75 patients of rectosigmoid cancer was analized. Results : Forty samples (34.2%) from tumors gave aneuploidy histogram. There was no significant difference in the frequency of DNA aneuploidy in terms of age, sex, depth of invasion, location and Dukes stage. But there was a significant correlation between DNA ploidy and the failure rates in Dukes stage B rectosigmoid cancer ( p=0.048). Conclusions : These findings suggest that DNA ploidy pattern shows the correlation with the treatment failure rates in Dukes stage B rectosigmoid, but not with many other clinicohistologic findings. However, more patients will be needed to disclose these findings. 목 적 : 여러 종양에 있어 DNA ploidy 양상은 여러 임상조직학적인 소견과의 연관성을 보여주어 왔다. 이에 본 연구에서는 대장직장암에 있어서의 이러한 연관성에 대해 알아보고 직장 S상 결장암에 있어서 치료 실패율과의 연관성에 대해 알아보고자 하였다. 대상 및 방법 : 본 연구에서는 대장 직장암으로 진단 후, 근치적 절제술을 시행받은 환자 117명을 대상으로 하였고 Hedley method에 따라 파라핀에 고정 후 flow cytometry 를 사용하여 DNA ploidy 와 여러 임상조직학적인 소견들과의 연관성을 밝히고자 하였다. 이 중 Duke 병기 B, C 직장 S상 결장암 환자 75명을 대상으로 하여 치료실패 양상과 DNA ploidy 간의 상관관계를 알아 보았다. 결 과 : 종양분석 결과 40예(34.2%)에서 aneuploidy histogram을 얻을 수 있었다. DNA aneuploidy 와 나이, 성별, 침범깊이, 위치 그리고 Dukes’ 병기와는 유의한 상관관계를 보이지 않았다. 그러나 Dukes 병기 B 직장암에 있어서는 치료 실패율과 DNA ploidy 사이에 유의한 상관관계를 보였다( p=0.048). 결 론 : 대장 직장암에서 DNA ploidy 는 다른 임상조직학적 소견들과 관련이 없었고 직장 S상 결장암 병기 B에서는 치료 실패율과 연관성을 보였으나 보다 많은 수의 환자를 대상으로한 검증이 필요하다고 생각된다.