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연구보문 : 자연과학 ; 새만금간척지에서의 녹비작물 재배 및 시용이 토양이화학성에 미치는 영향
강종국 ( Jong Gook Kang ),이상훈 ( Sang Hun Lee ),이경보 ( Kyeong Bo Lee ),이경도 ( Kyung Do Lee ),길근환 ( Geun Hwan Gil ),류진희 ( Jin Hee Ryu ),박기훈 ( Ki Hun Park ),이수환 ( Su Hwan Lee ),배희수 ( Hee Soo Bae ),황선아 ( Seon 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.1
새로 조성된 간척농지의 토양개량을 위하여 새 만금간척지에서 2007년부터 2011년까지 4년 동안 녹비작물 (여름철: 세스바니아, 제주재래피, 수수×수단그라스, 겨울철: 귀리, 호밀, 보리) 재배 및 시용이 토양이화학성에 미치는 영향을 조사하여 얻은 결과는 다음과 같다. 1. 녹비작물의 바이오매스 생산량은 평균 9.7 ~ 15.0 ton/ha이었으며, 이 중에서 수수×수단그라스의 생산량이 가장 많았다. 2. 녹비작물의 재배와 시용으로 토양유기물 함량이 1.3에서 6.0 g/kg로 매년 평균 1.2 g/kg씩 증가하였다. 3. 녹비작물의 재배와 토양환원에 의해 토양의 용적밀도가 1.44에서 1.24Mg/m3로 감소하였다. 4. 수수×수단그라스와 호밀의 이어짓기가 토양 용적밀도 감소에 가장 효과적이었고, 세스바니아 재배는 토양 질소함량 증가에 가장 효율적이었다. 이상의 결과로 미루어 볼 때 간척지에서의 녹비작물 재배 및 시용은 토양의 비옥도 요인을 일부 개선시킴으로써 간척농지 개량에 기여할 수 있을 것으로 기대된다. 앞으로 신간척지에서의 유기물과 관련된 다각적인 연구가 필요할 것으로 생각 된다. This study was conducted to investigate the effect of green manure crops on soil fertility and to provide informations for optimum cropping systems in Saemangeum reclaimed tidal land. Field trial was established with summer [sesbania (Sesbania sesban), wild millet (Echinochloa crus-galli), and sorghum-sudangrass hybrid (Sorghum bicolor)] and winter [oat (Avena sativa L.), rye (Secale cereale L.), and barley (Hordeum vulgare L.)] green manure crops at Saemangeum reclaimed tidal land in Korea from 2007 to 2011. Biomass of the green manure crops were 9.7-15.0 ton/ha (dry weight), and sorghumsudangrass hybrid produced consistently greater biomass than other crops across growing years. Cultivation and application of green manure crops increased soil organic matter contents from 1.3 to 6.0 g/kg by 1.2 g/kg per year. Green manure crops also improved bulk density from 1.44 to 1.24 Mg/m3 by addition of biomass residues. Sequential cultivation of sorghum-sudangrass hybrid followed by rye was most effective on decreasing bulk density of soil, and sesbania cultivation was most efficient on increasing soil nitrogen content. Overall, green manure crops can be probably useful for improvement soil fertility in Saemangeum reclaimed tidal land.
Soyoung Kang,Hye Jung Lee,김영호,Jung Hun Oh,Bum Jun Kim,Kook Jin Lim,Seunghwan Lee,황승용,이시혁,권덕호 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.3
Proteomic differences between Bemisia tabaci biotypes (B and Q) were investigated by two-dimensional gel electrophoresis in conjunction with mass spectroscopic analysis. Among several protein spots specific to biotype B, carboxylesterase 2 (Coe2) was significantly more expressed in biotype B. Phylogenetic analysis demonstrated the close relationship of Coe2 with Myzus persicae esterase E4. Comparison of full-length cDNA sequences of Coe2 revealed no amino acid differences in functionally important conserved regions between biotypes B and Q. The transcription level of the Coe2 gene (coe2) was 5.8-fold higher in biotype B than in biotype Q, but the coe2 copy number was not different between biotypes, suggesting that the overexpression of Coe2 was due to transcriptional up-regulation. Native isoelectric focusing followed by mass spectrophotometric analysis confirmed that the overexpressed pI 5.7 esterase in biotype B was Coe2. In-gel inhibition of Coe2 by three insecticides indicated the interaction of Coe2 with chlorpyrifos-methyl oxon and permethrin,but not with imidacloprid. These findings suggest that overexpression of Coe2 in biotype B can confer chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration and hydrolysis,as seen in M. persicae E4. Finally, utility of Coe2 as a potential biotype-specific protein marker is discussed.