너구리의 狂犬病 發生 症例 報告

Eui Kyung Hwang(黃義卿),Jae Hoon Kim(金哉勳),Su Myung Bae(裵洙明),Jung Won Park(朴重元),Jong Tae Woo(禹鍾泰),Yong Dhuk Yoon(尹用德) 한국예방수의학회 1994 예방수의학회지 Vol.18 No.4

Rabies is an ancient disease of humans and animals which is still endemic in many parts of the world. Rabies had not been occured for 8 years from 1985 to 1992. But one dog in Kangwon province in 1993 and many dogs and cattle in Kyunggi and Kangwon province in 1994 were identified to be infected with rabies virus. Wild animals especially including raccoons and badgers which are distributed widely in above regions, therefore they were suspected to be the source of rabies. Two wild raccoons were captured from Kyunggi province and examined routinely by rabies diagnosis techniques. They showed all positive results to the rabies test such as histopathological findgings, indirect fluorescent antibody test, mose inoculation test and transmission electron microscopy. In the histopathological examinations Negri body were observed frequently from the neuronal cytoplasm in hippocampus as well as the Purkinje cells in cerebellum. The bullet-shaped rabies viral particles were observed from not only neurons in hippocampus but also acinar cells in mandibular salivary glands. The raccoon was confirmed to be one of the possible source of rabies in Korea.

Brief Communications and Case Reports : 비육유우에서 발생한 기종저의 병리학적 관찰

황의경(Eui Kyung Hwang),김재훈(Jae Hoon Kim),윤순식(Soon Seek Yoon),손현주(Hyun Joo Sohn),조윤상(Yun Sang Cho),유한상(Han Sang Yoo),박경애(Kyung Ae Park),진영화(Young Hwa Jean),최상호(Sang Ho Choi) 한국수의병리학회 1998 한국수의병리학회지 Vol.2 No.1

狂犬病 發生實態 및 對策 : 綜說

Eui Kyung Hwang(黃義卿) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.3

The first official report on rabies in animals was recorded in 1907 in Korea. The duration from 1907 to present was divided into three periods according to the numbers of rabies cases and epidemiological aspects such as species of rabid animals and regional distribution of the outbreak. The first period named ‘endemic stage of rabies’ was designated from 1907 to 1945. At that time, there were lots of rabies outbreaks in Korea. Rabies had been occurred from 100 to 900 animals annually. For the control of rabies the phenol-glycerine inactivated rabies vaccines were inoculated to only limited numbers of dogs, about 4% of whole all dog population, and average 18,600 stray dogs were removed annually. The second period named ‘decrease and elimination stage of rabies’ was from 1945 to 1992. In this period about average 32 cases of rabies occurred annually from 1950 to 1984 but there was no rabies outbreak for 8 years from 1985 to 1992. Prominent decrease of rabies cases were mainly relied upon increase of vaccination rate in dogs and significant decrease of stray dogs. The type of rabies of both the first and second period was typical urban type which rabies of dogs are most prevalent. The third period named ‘recurrence stage of rabies’ has been begun since 1993 when rabies was recurred in dogs at Chulwon county in Kangwon province neighbouring to demilitarized zone(DMZ). After recurrence of rabies all rabies cases were occurred within six counties, two in Kyunggi and four in Kangwon provinces, which were located near to DMZ. In species of rabid animals, cases of cattle exceeded cases of dogs and two wild raccoons captured from rabies recurring areas were identified to be infected with rabies virus. Therefore wild animals including raccoons were suspected to be a origin of rabies of cattle and dogs. This type of rabies outbreaks was considered as rural type (wildlife type) comparing to previous urban type. Eight counties, four in Kyunggi and four in Kangwon provinces, were designated as the first prevention zone to block spreading of rabies to other parts of the country and strict vaccination policy for dogs. and cattle within the zone has been carried out since 1994. Survey and research on rabies in wildlife within DMZ will be executed soon.

狂犬病 發生實態 및 對策

Eui Kyung Hwang(黃義卿) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.2

The first official report on rabies in animals was recorded in 1907 in Korea. The duration from 1907 to present was divided into three periods according to the numbers of rabies cases and epidemiological aspects such as species of rabid animals and regional distribution of the outbreak. The first period named ‘frequent outbreak stage of rabies’ was designated from 1907 to 1945. At that time, there were lots of rabies outbreaks in Korea. Rabies had been occurred from 100 to 900 animals annually. For the control of rabies the phenol-glycerine inactivated rabies vaccines were inoculated to only limited numbers of dogs, about 4% of all dogs, and average 18,600 stray dogs were eliminated annually. The second period named ‘decrease and elimination stage of rabies’ was from 1945 to 1992. In this period about average 32 cases of rabies occurred annually from 1950 to 1984 but there was no rabies outbreak for 8 years from 1985 to 1992. Prominent decrease of rabies cases were mainly relied upon increase of vaccination rate in dogs and significant decrease of stray dogs. The type of rabies of both the first and second period was typical urban type which rabies of dogs are most prevalent. The third period named ‘reoutbreak stage of rabies’ has been begun since 1993 which rabies was reoccurred in dogs at chulwon county in Kangwon province neighbouring to demilitarized zone(DMZ).. After reoutbreak of rabies all rabies cases were occurred within six counties, two in Kyunggi and four in Kangwon province, which were located nearly to DMZ. In species of rabid animals, cases of cattle exceeded cases of dogs and two wild raccoons captured from rabies reoccurring areas were identified to be infected with rabies. Therefore wild animals including raccoons were suspected to be a origin of rabies of cattle and dogs. This type of rabies outbreaks was considered as rural type(wildlife type) comparing with previous urban type. Eight counties, four in Kyunggi and four in Kangwon province, were designated as the first prevention zone for blocking of rabies outbreaks in other parts of the county and strict vaccination policy for dogs and cattle has been carried out since 1994. Survey and research on rabies of wildlife in DMZ will be executed soon.

狂犬病 診斷法 및 豫防藥의 發展 : 綜說

Eui Kyung Hwang(黃義卿) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.3

Rabies outbreak had been occurred in both human and animals even before the first official report on rabies in animals recorded in 1907 in Korea. Before early 1950’s the diagnosis for majority cases of animal rabies was dependent upon clinical signs and epidemiological characteristics such as excitement, salivation, dysphagia and bite by suspected rabid animals. After middle of 1950’s Seller’s staining method, mouse inoculation(MI) test, histopathological examination of brain lesions and complement-fixation test were used for diagnosis of animal rabies. But the complement-fixation test was not used officially but only experimentally for a short period. Direct fluorescent antibody test using hyperimmune serum was developed in 1970 and this method was used as official standard method for diagnosis of rabies until 1987. In 1988, as a result of production of monoclonal antibody against rabies virus, direct fluorescent antibody test was substituted by indirect fluorescent antibody(IFA) test. IFA test, MI test and histopathological examination have been used as official standard methods for the diagnosis of rabis in Korea.

강원도 사육 한우에서 Neospora caninum에 대한 항체양성률 조사

황의경(Eui Kyung Hwang) 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

돼지 Balantidiasis의 병리학적관찰

황의경,권영방,정운익,강승원,Hwang Eui-kyung,Kwon Young-bang,Chung Un-ik,Kang Seung-won 대한수의사회 1984 대한수의사회지 Vol.20 No.11

Severe ulcerative colitis and cecitis by Balantidium coli was studied pathologically in a 40 day old male pig. Grossly, many variable-sized ulcers were diffusely scattered on the mucosa of the cecum and the colon. Microscopically, numerous balantidia pene

PCR-RFLP 기법을 이용한 Porcine Stress Syndrome의 진단

황의경,김연수,Hwang, Eui-Kyung,Kim, Yeon-Soo 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.1

We have utilized the PCR-RFLP method to detect the ryanodine receptor(RYR1) gene mutation and to estimate the genotype frequencies of the RYR1 gene in commercial crossbred pig population. The exon region(659bp) including point mutation(C ${\rightarrow}$T; Arg ${\rightarrow}$Cys) in the porcine ryanodine receptor gene, which is a causal mutation for PSS, was amplified by PCR and digested with Cfo I restriction enzyme. The RYR1 gene was classified into three genotypes by agarose gel electrophoresis. The normal homozygous(NN) individuals showed two DNA fragments consisted of 493 and 166bp. The mutant homozygous(nn) individuals showed only one DNA fragment of 659bp. Also, all three fragments(659, 493 and 166bp) were showed in heterozygous(Nn) carrier animals. The proportions of normal, carrier and PSS pigs within crossbred population of pigs were 81%, 15% and 4%, respectively. According to the results of analysis of variance for the association of genotypes of RYR1 of pigs at 30kg, day age at 90kg and average daily gains, the RYR1 nn genotype was very higher than RYR1 NN genotype for day age at 30kg with 5% level of significant difference, but no significant difference for association of any other genotypes with day age at 90kg and average daily gain in crossbred pigs. Therefore, DNA diagnosis by using PCR-RFLP analysis for the PSS gene was useful for large-scale screening of commercial pigs in the swine industry.

Arbitrary-Primed PCR 기법을 이용한 Salmonella 균의 다형성 분석

황의경,김상균,김연수,김우태,이정구,Hwang, Eui-Kyung,Kim, Sang-Kyun,Kim, Yeon-Soo,Kim, Woo-Tea,Lee, Jeong-Koo 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2

이 연구는 AP-PCR 기법을 이용하여 Salmonella 균주의 유전적 다양성과 근연관계를 비교하고자 수행하였다. 18종의 Salmonella 균주에 대해 8종류의 primer를 이용하여 각 균주에 대한 DNA 밴드를 검출한 결과 총 AP-PCR 단편의 수는 39에서 52개의 범위였으며 평균 43.6개가 검출되었다. 총 349개의 표지인자가운데 다형성을 나타내는 단편들은 185개로서 53.0%의 다형성 수준을 보여주었다. 살모넬라균주들은 GEN 703과 GEN 708 primer에서 각각 0.682와 0.676의 높은 다형성 수준을 보여주었으나 GEN 603, GEN 604, 및 GEN 607 primer에서는 각각 0.404, 0.460 및 0.472의 비교적 낮은 수준의 다형성을 나타냄으로써 살모넬라균주간 상동성 비율이 높음을 시사해 주고 있다. 따라서, 이들 primer들은 Salmonella 균주들의 AP-PCR 분석에 대단히 효율적임을 확인할 수 있었다. S typhimirium CU 2001의 다형성 수준은 77%로 S typhimirium CU 2002와 가장 근연관계가 가까운 것으로 나타났으며 다음이 S typhimirium CU 2003으로 63%, S typhimirium ATCC 14028이 50%, 그리고 S typhimirium CU 2004가 43%의 근연관계를 나타내었다. S enteritidis ATCC 13076의 다형성 수준은 83%로 S enteritidis CU 2005와 근연관계가 가장 가깝게 나타내었으며 다음이 S enteritidis CU 2006으로 63%, S enteritidis CU 2007이 58%의 근연관계를 나타내었다. S choleraesuis CU 2009의 다형성 수준은 67%로 S choleraesuis CU 2010과 가장 가까운 근연관계를 나타내었으며 S choleraesuis CU 2008은 53%의 근연관계를 나타내었다. S gallinarum CU 2011과 S gallimarum CU 2012의 다형성 수준은 70%로 근연관계가 가장 가깝게 나타났으며 S gallinarum ATCC 9184는 60%의 근연관계를 나타내었다. 마지막으로 S pullorum CU 2013과 S pullorum CU 2014의 다형성 수준은 80%로 매우 높은 근연관계를 나타낸 반면 S pullorum No 11은 53%로 근연관계가 먼 것으로 나타났다. 따라서, AP-PCR 분석은 Salmonella 균주의 유전적 다양성 및 근연관계를 추정하기 위한 강력한 도구로서 이용할 수 있을 것이다. In this study, eight primers were used to detect genetic variability and phylogenetic relationships among the eighteen Salmonella strains by the arbitrary-primed PCR(AP-PCR) techniques. Five strains of Salmonella typhimurium, four strains of S entertidis, three strains of S choleraeuis, three strains of S gallinarum and three strains of S pullorum were typed by AP-PCR. The number of AP-PCR bands detected per each primer varied from 39 to 52, with an average of 43.6. A total of 349 AP-PCR bands were generated and among them, 185 bands(53.0%) were polymorphic. Among the primers, GEN 703 and GEN 708 primer showed a high level of polymorphism with 0.682 and 0.676, respectively. But GEN 603, GEN 604 and GEN 607 primer showed a low level of polymorphism with 0.404, 0.460 and 0.472, respectively. Therefore, the these primers will be the most effective for AP-PCR analysis of Salmonella strains. The level of polymorphism of S typhimurium CU 2001(0.77) was similar to that of S typhimurium CU 2002(0.77) and lower than those of other strains such as S typhimurium CU 2003(0.63), S typhimurium ATCC 14028(0.50) and S typhimurium CU 2004(0.43). The level of polymorphism of S enteritidis ATCC 13076(0.83) was similar to that of S enteritidis CU 2005(0.83) and lower than those of other strains such as S enteritidis CU 2006(0.63) and S enteritidis CU 2007(0.58). The level of polymorphism of S choleraeuis CU 2009(0.67) was similar to that of S choleraeuis CU 2010(0.67) and higher than those of other strains such as S choleraeuis CU 2008(0.53). The level of polymorphism of S gallinarum CU 2011(0.70) was similar to that of S gallinarum CU 2012(0.70) and higher than those of other strains Such as S gallinarum ATCC 9184(0.60). The level of polymorphism of S pullorum CU 2013(0.80) was similar to that of S pullorum CU 2014(0.80) and higher than those of other strains such as S pullorum No 11(0.53). Therefore, the AP-PCR analysis will be used a powerful tool for estimating genetic variation and phylogenetic relationships among Salmonella strains.

강원도 사육 젖소의 네오스포라포자충(Neospora caninum)에 대한 항체양성률 조사

황의경,Hwang, Eui-Kyung 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.1

This survey was carried out to investigate the seroprevalence of antibodies to Neospora (N.) caninum in dairy cattle raised in Kangwon province in Korea. A total of 349 sera collected from dairy cattle were tested for N. caninum antibodies using an indirect immunofluorescent antibody test (IFAT). Ninety six (27.5%) dairy cattle were positive by IFAT. Seroprevalence of cows was 28.9% (91/315) and seroprevalence of boars was 14.7% (5/34). The seroprevalences of cows were increased according to the ages from 19.6% in cows less than 2 year-old to 50.0% in cows more than 7 year-old. Among the seroprevalences of cattle according to the raised areas, five counties or cities, Wheongsung was 34.6% (27/78), Wonju was 29.3% (46/157), Hongcheon was 28.9% (13/45), Chuncheon was 15.0% (3/20) and Cheolwon was 13.3% (2/15). It was proved that dairy cattle raised in Kangwon provinces exposed extensively and seriously to N. caninum.