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Edwardsiella tarda의 특이 Bacteriophage와 Bacillus subtilis KM-1혼합액이 Edwardsiella tarda 에 미치는 항균효과
백민석 ( Min Suk Baek ),황요셉 ( Yo Sep Hwang ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3
본 연구는 E. tarda에 대한 특이박테리오phage와 생균제의 일종인 B. subtilis KM-1의 혼합제가 E. tarda에 대한 항균활성에 미치는 효과를 알아보고자 수행되었다. 가장 효과적인 혼합제의 항균활성을 보인 phage의 적정 수는 B. subtilis 가 2×105 CFU/㎖의 농도로 존재할 때 배양 36시간째 2×105 PFU/㎖로 나타났다. B. subtilis의 경우 2×105 PFU/㎖의 phage가 존재할때 첨가량에 비례하는 항균활성을 보여주었다. phage와 B. subtilis의 혼합투여는 phage나 B. subtilis 각각을 투여한 그룹보다 훨씬 높은 항균활성을 나타냈다. 본 연구의 결과는 수산양식에서 대두되고 있는 양식어류의 E. tarda 감염성 질병 치료를 위한 항생제 대체 제로서의 사료첨가제 개발 가능성을 제시하고 있다. The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was 2×105 PFU/㎖ with 1×10 CFU/㎖ of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage (2×105 PFU/㎖). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
백민석 ( Min Suk Baek ),황요셉 ( Yo Sep Hwang ),최상훈 ( Asang Hoon Choi ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.1
The present study investigated the effects of dietary Edwardsiella tarda (E. tarda) specific bacteriophage (phage) and Bacillus subtilis (B. subtilis) mixture on innate immune responses and antibacterial activity of Nile tilapia, Oreo-chromis niloticus. In a dietary experiment, tilapia were fed the control diet (C), a phage-only supplemented diet (P), a B. subtilis only supplemented diet (B), or a B. subtilis and phage mixed diet (B+P). A respiratory burst and significant increase in lysozyme activity (P <0.05) were noted in the B+P group, as compared to other groups after 4 days of feed- ing. The B group showed a significant (P <0.05) increase in respiratory burst and lysozyme activity versus the C and P groups, whereas no significant increases (P <0.05) were observed in the P and C groups. ACH50 was significantly up-regulated in the B+P group versus other groups after 8 days of feeding (P <0.05). In vivo antibacterial activity was significantly enhanced in the B+P fed group, as compared to other groups (P <0.05) after 7 days of E. tarda challenge. A significant (P <0.05) increase in antibacterial activity was seen in the B group, as compared to C or P groups after 14 days of feeding. These results suggest that a B. subtilis and phage mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
이재훈 ( Jae-hoon Lee ),권대근 ( Dae-geun Kwon ),황요셉 ( Yo-sep Hwang ),신현근 ( Hyeon-geun Sin ),정욱조 ( Uk-jo Jeong ),양지연 ( Ji-yeon Yang ),한광혁 ( Gwang-hyeok Han ),임양원 ( Yang-won Lim ),임한규 ( Han-kyu Lim ) 한국정보처리학회 2013 한국정보처리학회 학술대회논문집 Vol.20 No.2
최근 스마트폰 보급률이 높아지면서 장애인들에 대한 스마트폰 보급도 올라가게 되었다. 하지만, 스마트폰을 이용하기 위한 수많은 어플리케이션이 출시되고 있는데 비해 장애인들에게 필요한 어플리케이션은 많지 않다. 또한 장애인들을 위한 어플리케이션은 그 조작법이 어렵거나 사용자가 필요로 하는 기능이 부족하다. 특히, GPS기능을 활용한 유용한 앱들이 많은 반면 장애인들을 위한 내비게이션 앱은 부족하다. 본 논문에서는 청각장애인들이 쉽게 접근할 수 있도록 청각장애인을 위한 길찾기 어플리케이션에 청각장애인들이 유용하게 활용할 수 잇도록 청각장애인용 앱을 설계하였다.
Vibrio alginolyticus에 대한 특이 bacteriophage의 생물학적 특성
허용주 ( Yong Ju Heo ),이찬흔 ( Chan Heun Lee ),백민석 ( Min Suk Baek ),안현미 ( Hyun Mi Ahn ),황요셉 ( Yo Sep Hwang ),박권삼 ( Kwon Sam Park ),최상훈 ( Sang Hoon Choi ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6
Vibrio alginolyticus, a marine f ish and shellf ish pathogen, has been found at a high frequency around the coastal areas of Korea. Vibrio alginolyticus was purif ied from various diseased f ish, and a V. alginolyticus-specif ic bacteriophage was isolated from seawater obtained from f ish farms located on the west coast of Korea. In a bacterial lysis experiment using the phage and antibiotics, tetracycline, 103 cfu/ml of V. alginolyticus were completely lysed by both the phage and the antibiotic, suggesting that the purif ied phage in the study could be utilized as an alternative to antibiotics in the control of f ish and shellf ish diseases caused by V. alginolyticus.
서해안 양식장에서 분리한 Vibrio alginolyticus의 특이 bacteriophage에 대한 구조적 특성
허용주 ( Yong Ju Heo ),이찬흔 ( Chan Heun Lee ),백민석 ( Min Suk Baek ),안현미 ( Hyun Mi Ahn ),황요셉 ( Yo Sep Hwang ),박관하 ( Kwan Ha Park ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Vibrio alginolyticus (V. alginolyticus), which is one of bacterial pathogens evoking severe infection in fish and shellfish as well as in human has been found at high frequency around all coast areas in Korea. Both V. alginolyticus and V. alginolyticus specific bacteriophage were isolated from sea water and various fishes from fish farms on west coast in Korea. In a morphological study based on electron microscope, the purified phage appeared to be composed of hexagon head of 60 nm and short tail of 20 nm. In the denatured SDS-PAGE analysis, the structural proteins of the phage were found to be 7 different protein fractions ranging from 37.8 to 198 kda. The kind of nucleic acid of the phage was ascertained to a double stranded DNA.