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이성진(S . J . Lee),황규춘(K . C . Hwang),최강덕(K . D . Choi),이학교(H . K . Lee),신영수(Y . S . Shin),한재용(J . Y . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.3
This study was performed to analyze genetic characteristics of Korean Native Ogol Chickens compared with various breeds of chickens including Korean Native Yellow Chickens, White Leghorns and Rhode Island Reds. For DIVA fingerprinting, we collected blood samples from each of 10 individuals per breeds and obtained the genomic DNA. The genomic DNA samples were digested with restriction enzymes(Hinf I, Hcre III. etc.) and hybridized with various probes(Jeffreys` probes 33.15, 33.6, and M13) after Southern transfer. Probe 33.1 gave only a few unresolvable bands, however, probe 33.6 and M l3 detected a large number of bands and was thought to be the desirable probes for the analysis of genetic characteristics for various breeds of chickens. The use of pooled DNA enabled us to significantly reduce the number of DNA samples for estimation of genetic relationships among breeds. Furthermore, the results of DFP prepared from pooled DNA were similar to those of DFP prepared from individual DNA. In this analysis, KNOC was estimated to be a highly inbred line (band sharing $gt; 0.69), and to be closest in genetic distance to KNYC than any other bred.
한국 오골계의 유전 특성 분석을 위한 임의 염기 서열의 Short Oligonucleotide Primer 를 이용한 Polymerase Chain Reaction 기법의 적용
황규춘,한재용,손시환,홍영호,이학교,조병욱 한국축산학회 1997 한국축산학회지 Vol.39 No.1
This study was carried out to establish the optimal RAPD condition using, short oligonucleotide primers and then to compare the genetic characteristics of Korean Native Ogol Chicken with other breed of chicken, including WL, RIR, CN and KNBC. Each 5 individuals per breed were bled and DNA was extracted from collected blood. The primers used in this study were composed of 10 oligonucleotides of 50∼80% G+C content. The optimization on the primer annealing temperature, concentration of Mg ion, amount of template DNA was conducted. From initial 400 primers, 50 polymorphic primers between individuals of same or different breeds were selected. And finally, 10 primers showing highly polymorphic and reproducible pattern were selected and 49 polymorphic markers were counted for Band Sharing Coefficient estimation. Both KNOC and KNBC showed a high genetic homogeneity (0.862, 0.858) and RIR showed most homogeneous genetic composition {0.891). In the case of interbreed comparisons, both KNOC and KNBC showed the highest genetic similarity with CN (0.810, 0.835) and the least with WL (0.772, 0.782) than any other breeds. Therefore, the RAPD technique using short oligonucleotide sequences was useful for genetic analysis in chicken
한우의 경제형질들과 관련한 유단백 유전자와 성장호르몬 유전자의 효과
이득환,황규춘,한재용,전광주,이학교,조병욱 한국축산학회 1998 한국축산학회지 Vol.40 No.2
Milk protein genes and growth hormone gene have been targeted as modulator genes in growth and carcass traits. With the objectives to detect some correlation or genotypic effect of three known functional genes on economic performance, 19 sires and their 139 progenies of Hanwoo population were genotyped for κ-casein, β-lactoglobulin and growth hormone(GH) loci by polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP) analysis. The growth traits, such as Body Weight and Average Daily Gain, and carcass traits of this population were analysed for possible association with three genetic loci. The frequency of B allele for κ-casein was 0.37 in Hanwoo and 0.23 for β-lactoglobulin. Among the analysed loci, κ-casein gene was found most significantly associated with the growth traits of economic importance. However, the ,β-lactoglobulin and GH did not show significant effects in Hanwoo. For carcass traits, effects of κ-casein were significant in EMA. Therefore, genotyping the candidate animals for κ-casein in Hanwoo can be of great benefit in selection programs.