굴곡부 망상 색소이상증

황규왕(Kyu Uang Whang),이창우(Chang Woo Lee),김중환(Joong Hwan Kim) 대한피부과학회 1985 대한피부과학회지 Vol.23 No.4

Reticulated pigrnented anomalies of the flexures is a pigmentary disorder bearing genetic back ground, also known as Dowling Degos disease or dark dot disease. It is characterized reticulated brownish black macules on the flexural areas with the histological features of filiform pigmented epidermal downward proliferation and the presence of intraepidermal keratin containing cysts. We report 34-year-old housewife who showed brawnish black reticulated macules on her axillae, anterior neck and genitocural folds. Pigmented comedones were also found at the marginal areas of the axillae. Biopsy specimen revealed the presence of intraepidermal keratin containing cysts and basilar hyperpigmentation.

건선의 조직 T 임파구에 관한 연구

황규왕(Kyu Uang Whang),이창우(Chang Woo Lee),손숙자(Sook Ja Son),김재홍(Jae Hong Kim),김중환(Joong Hwan Kim) 대한피부과학회 1985 大韓皮膚科學會誌 Vol.23 No.3

Anti-T cell monoclonal antibodies(OKT series: OKT4a OKT8A, OKT1 1) ir,amunoperoxidase technique studies were performed for the presence of T cells and their subpopulations in cutaneous lesions of early papules and late plaques from 10 patients with psoriasis. In this semiquantitative examination, T cells reactive to OKT11 antiserum constituted about 70 percents of total leukocytes found in the dermis, both in early papular lesions and late plaque lesions. The number of helper/inducer T cells was about equal to the number of suppressor/cytotoxic T cells in early papular lesions, but helper/inducer T cells were predominant in plaque lesions. Although in situ functions of T cell and their subpopulations can not precisely be deduced, this different proportions of the helper/inducer and suppressor/cytotoxic T cells found in this study might be related to the immunopathologic mechanisms pertainmg to the persistence and the expansion of psoriatic lesions.

임질의 항생제 병용 요법 - Kanamycin 과 Penicillin 병용 요법과 Kanamycin 과 Trimethoprim - Sulfamethoxazole 병용 요법의 비교 -

황규왕(Kyu Uang Whang),김재홍(Jae Hong Kim),김중환(Joong Hwan Kim) 대한피부과학회 1986 대한피부과학회지 Vol.24 No.5

From August to November 1985 244 bacteriologically proven male uncomplirated goncicoccal urethritis patients at the VD clinic of Choong-Ku Public Health Center form August to November 1985 were divided into group A and group B according to a random number sheet. In group A, treated with kanamycin 2.Ogm, im plus benzyl penicillin-G 5 mega units, im plus probenecid, 1.Ogm, PO; 112 of 121 patients were followed and 10 patients (8.9%) failed to be recovered. In group B, treated with kanamycin, 2,Ogm, im plus trimethoprim-sulfamethoxazole, 9 tahlets, PO; 112 of l23 patients were followed and 7(6.3%) failed. There is no sign.ificant difference between the two groups (p>0. 05) The failure rates in PPNG urethritis were 14.3% and 8.0% in group A and group B respectively. There is a signficant difference in failure rate between the two groups (P<0.05). It is suggested that, because of high rate of PPNG among circulating N.gonorrhoeae, the combinatioin regimen of kanamycin and trime.thoprim-sulfamethoxazole may be used as a first line treatrnent regimen for uncomplicated gonococcal urethritis

인체 각질형성세포와 KB 세포에서의 Fas 항체 , IFN - γ , IL - 1α에 의한 apoptosis 유발에 관한 연구

이홍규,황규왕,박영립,김영근,( Hong Kyu Lee,Kyu Uang Whang,Young Lip Park,Young Keun Kim,David A Norris ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.2

Background: The Fas antigen is a cell surface molecule that mediates apoptosis in many cell types. Matsues group indicated that keratinocytes constitutively express the Fas antigen and apoptosis was induced only on pretreatment with interferon-r(IFN-y) in cultured normal human keratinocytes(NHK). Objective : We undertook this study to determine the induction of apoptosis by Fas antibody alone and/or in combination with IFN y, IL-1a in normal human keratinocytes(NHK) and transitional epithelioma cell lines(KB cell) which had lower levels of intracellular IL-1 receptor antago- nists(IL-1ra ). Methods : We used cultured NHK and KB cells. Each cell was treated with IFN-r, IL-la and Fas antibody for induction of apoptosis. For quantifying the apoptosis, index fluorescent DNA- binding dyes were used. Result: Fas antibody alone could induce apoptosis not only in KB cells but also in NHK cells. The combination of Fas antibody and IFN-r enhanced the induction of apoptosis in NHK and KB cells. The IL-la alone could induce apoptosis only in KB cells which had relatively small amounts of IL-1ra compared to NHK. Conclusion : Our result may indicate that Fas antigen in human keratinocytes can regulate normal epidermal cellular differentiation and proliferation. (Kor J Dermatol 1997;35(2): 273-278)

레티노이드에 의한 악성 흑색종 세포주의 bcl - 2 발현에 관한 연구

방성원,박영립,황규왕 ( Sung Won Bang,Young Lip Park,Kyu Uang Whang ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6

Background: Apoptoss plays a major role in cellular proliferation and differentiation in tumor cells. Bcl-2, proto-oncogene, is known to inhibit apoptotic cell death of tumor cells. The high expression of bcl-2 in human melanoma cells over transforrned keratinocytes has been reported. The Loton group indicated that the growth of human melanoma cells exposed to ret.inoids was inhibit ed and their cellular melanin content incrensed over that of the untreat,ed ce1Ls. The Veis group reported that bcl-2 defieient mice showed hypopigmented hair. Which suggests that bcl-2 may in volve melanogenesis. The Above mentioned findings may suggest that. bcl-2 and retinoids may play a role in melanoms biology. Objective : We under ook this study to elucidate a possible relationship between retinoids and bcl-2 expvessions in human melanoma cell lines. Methods : We analysed bcl-2 expressions from SK 28 cells(melanoma cell lines) after pretreat ment with retinoids using flow cytometry and imtnunoblotting. Results : 1. In the results of the preliminary studies, we found that cultured human keratinocytes, fibro blasts and melanocytes n the resting state showed expressions of bcl-2. The latter showed a four fold expression of bcl-2. 2. Expression of bcl-2 was detected in SK 28, a human melanoma cell line, in the resting state. 3. After incubation with isotretinoin or etretinate treatment at 37'C, for 48 hours, this treated group showed a more ir creased expression of bcl-2 than the control group. Conclusion : Our data may explain that the mechanism of ret.inoids indur,ing inhibition of mela noma cell growth may be partly due to upregulation of bcl-2 expression. The high base-line ex pression of bcl-2 in melanoma cells may tell us why these pigment cells can survive against oxi dative products generated during melanogenesis. (Korean J Dermatol 1997;35(6): 1088-1094)

수종 국소향균제의 배양 인체 각질형성세포 및 섬유아세포에 대한 세포독성도의 비교연구

표형찬,김영근,황규왕,박영립,은희철 ( Hyung Chan Pyo,Young Keun Kim,Kyu Uang Whang,Young Lip Park,Hee Chul Eun ) 대한피부과학회 1995 대한피부과학회지 Vol.33 No.5

현재까지 국소 항균제의 피부 도포는 이들의 생체에 미치는 영향 즉 독성도가 거의 무시된 채 사용되었고 사실상 정상적인 피부에는 여러가지의 장벽이 있어 이들이 직접적으로 피부에 많은 손상을 줄 것으로 생각되지는 않는다. 그러나 최근 화상 환자의 피부 이식에 배양된 각질형성세포의 이용이 증가되고 있고, 여러 피부 질환들에서는 정상적인 피부 방어기전이 손상되어 있으므로 국소 항균제를 사용할 경우 이들의 직접적인 세포독성을 알아볼 필요가 있다고 본다. 세포 배양을 하여 세포 독성을 알아보는 데는 섬유아세포 배양등 배양이 배교적 용이한 세포를 사용하는것이 편리하다고 생각할 수 있겠으나, 국소 항균제의 경우에는 항균제에 의해 직접적으로 자극을 받는 각질형성세포를 이용하는 것이 가장 이상적이라고 할 수 있으며 상처 치유에 밀접한 관련이 있는 섬유아세포와 비교해 보는 것도 의의있다고 하겠다. 정상 인체의 각질형성세포 배양은 배양 조건이 까다로와 이의 사용이 어려웠으나 비교적 최근에 와서 그 배양법이 정립되어, 이러한 계통의 연구에 이용되고 있다. Feeder 세포와 혈청을 첨가한 초기의 각질형성세포 배양법은 그 후 feeder 세포를 제거하고, collagen-coated dish를 사용한 방법들로 변형되어 왔고 그 이후에 개발된 방법으로 feeder 세포와 혈청을 사용하지 않는 방법이다. 세포 배양을 이용하여 피부 독성을 판별하는 방법으로는 배양세포에 약제를 처리한 후 세포 배양액 중에 증가나 감소되 화학 물질의 측정이 사용될 수 있다. 이 방법은 기능적인 변화를 알아볼 수 있는데 유효하다고 알려져 있다. 반면 어떤 물질의 직접적인 세포 독성을 알아 보는데는 세포 수의 계산이나 동위원소를 사용한 방법들이 사용된다. 동위원소의 사용은 안전도가 항시 문제되며 [^3H]-thymidine incorporation 측정법은 주로 종양세포 등의 빠른 증식을 보이는 세포들에서 사용되는데 정상 각질형성세포에서도 측정된 바 있다. 세포배양을 이용한 국소항균제의 독성도를 측정하는 방법은 형태학적 변화를 현미경적으로 관찰 하거나 살아있는 세포를 염색하여 세포수를 계산하는 방법이 가장 많이 사용되었으나 전자는 너무 주관적인 단점이있고, 후자는 부정확하고, 많은 표본을 동시에 시행하기 힘든 단점이 있다. 그에 비해 Mosmann(1983)이 처음 사용한 후 암 억제 물질의 선별 검사법으로 널리 사용되고 있는 3-4, 5-dimethylthiazol-2, 5-diphenyltet razolium bromide test(이하 MTT라 약함)는 살아있는 세포의 미토콘드리아에서 tetrazolium dye를 formazan 이라는 색소로 변환시켜 이 색소변화를 ELISA용 흡광도측정기로 신속한 판독이 가능한, 간편하고 빠르며 sensitivity가 비교적 좋은 방법으로 알려져 있다. 세포배양을 이용한 항균제의 독성도의 측정에 MTT를 이용한 방법은 최근 Tatnall(1990) 등이 섬유아세포와 각질형성세포에서 비교 연구한 바가 있고, Damour 등15이 화상 환자에 주로 사용하는 항균제들을 대상으로 세포독성도를 측정한 바가 있을 뿐이며 과거 타 저자들은 주로 세포 수의 단순계산(Coulter count) 방법을 사용하였다. 심근 경색의 진단 및 기타 질환의 진단에 널리 사용되고 있는 lactic dehydrogenase(이하 LDH)의 측정은 세포막 손상의 지표로 이용되고 있는데 최근 배양각질형성세포의 세포독성 측정에도 이용되고 있으나 항균제의 독성측정에 이용된 바는 없었다. 임상적으로 널리 사용되고 있는 대부분의 국소 항균제들은 소독제 및 국소 항생제들로 창상 치유, 수술 전후 처치, 기타 여러 무균적 조작이 필요 시 소독 및 살균 목적으로 혼용되고 있는데 소독제로서는 chlorhexidine, hydrogen peroxide, povidone iodine, ethanol용액과 화상환자에서 주로 사용되는 sulfadiazine 연고제와 국소 항생제 연고로는 gentamicin, fusidic acid, bacitracin 등이 가장 많이 사용된다. 본 연구의 목적은 정상인의 피부에서 배양된 각질형성세포와 섬유아세포에 가장 많이 사용되고 있는 국소 항균제를 여러농도로 처리한 후 세포의 생존력을 알아 보는 MTT방법과 세포막 손상의 지료로 사용되는 LDH 측정법 및 DNA 합성을 보는 [^3H]-thymidine incorporation 방법을 함께 시도하여 여러 항균제의 세포독성도를 종합적으로 측정 분석하고 두 세포간의 차이 및 각 항균제간의 성분 및 농도별 차이를 비교하여 독성이 적은 국소 항균제를 선별하고 그것의 사용 농도를 조사하는데 있다. The clinical use of topical antimicrobial substances is common in clean wounds as well as contaminated and chronic wounds. This use is based on the observation that the reduction of superficial bacterial contamination promotes wound healing. While the antibacterial efficacy of topical antimicrobials has been well documented, their effects on living tissue and the process of wound healing remain controversial. Recently, there have been several trials to detect the toxicity of variousantimicrobial materials by using cultured fibroblasts and keratinocytes. In this study the cytotexicity of several commonly used antimicrobials and the cytotoxic concentrations of antimicrobials on cultured human keratinocytes and fibroblasts were compared using 3 different methods:1) the MTT method showing the ability of the cells to clear the cytotoxic agents, 2) LDH release indicating damage of cell membrane, and 3) tritiated thymidine incorporation measuring DNA synthesis. In the MTT method all topical antimicrobials in their usual clinical concentration showed cytotoxicity on both keratinocytes and fibroblasts by suppressing cell proliferation. Keratinocytes were more sensitive to certain antibiotics. When the 50% inhibition concentration(IC50) of topical antimicrobials were compared, the cytotoxicity to keratinocyte was, in the decreasing order, chlorhexidine, hydrogen peroxide, povidone iodine, sulfadiazine, fusidic acid, ethanol, gentamicin and bacitracin. The cytotoxicity to fibroblasts was, in decrasing order, chlorhexidine, hydrogen peroxide, povidone iodine, fusidic acid, ethanol, sulfadiazine, bacirtracin, and gentamicin. Most of the antimicrobials tested increased LDH release by keratinocyte in IC50. Chlorhexidine, hydrogen peroxide and povidone iodine showed statistically significant increase in LDH release. The tritiated thymidine incorporation study showed the order of cytotoxicity to keratinocyte in IC50 of each antimicrobials was similar to that of the MTT method. Tritiated thymidined incorporation, However, appeared to be a more sensitive method in detecting cytotoxicity than MTT because the IC50 was lower in the former. In conclusion, all topical antimicrobials are cytotoxic to keratinocytes and fibroblast in their usual concentration. Because of their cytotoxicity it is suggested that one should use topical antimicrobials in a dilution of up to 1:1,000 of commonly used concentration for a short period only especially when applied directly on a skin graft or wounds without a protective barrier. Bacitracin or gentamicin appear to be less cytotoxic among all the antimicrobials tested.(Kor J Dermatol 1995;33(5):895∼906)

난치성 주사에 있어서 13 - Cis - Retinoic Acid의 치료경험

허훈(Hoon),황규왕(Kyu Uang Whang),이창우(Chang Woo Lee),김재홍(Jae Hong Kim),김중환(Joong Hwan Kim) 대한피부과학회 1986 大韓皮膚科學會誌 Vol.24 No.2

Eight men with a one-ta-seven-year history of rosacea which poorly controlled by oral tetracycline or metronidazole, with or without topical medications, were given 13-cis-retinoic acid in dosages of p.5 to 0. 7 mg/kg/day for eight weeks. The size of erytherna and the number of papules had been gradually decreased since the second week of treatment. Eight weeks later more than 75p, regression was recognized in six patients, however telangiectasia remained unchanged. All eight patients complained of only mild degrees of cheilitis and dryness of the nose, and had no evidence of the serologic abnormalities on the tests examined during the period of therapy.

만성 신부전증 환자에 동반된 후천성 천공성 질환 4예

방성원,부태성,황규왕,정현,김영근 ( Sung Won Bang,Tai Sung Bu,Chung Hyun,Kyu Uang Whang,Young Keun Kim ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Acquired perforating disease(APD) is characterized by hyperkeratotic papules with transepidermal elimination of degenerated material and is associated with renal disease and/or diabetes. Particular attention has been directed to transepidermal elimination because the articles on perforating diseases among patients with chronic renal failure and/or diabetes have been increasingly reported. We describe four patients with chronic renal failure and/or diabetes whose skin biopsy specimens showed transepidermal elimination. (Kor J Dermatol 1997;35(2): 333-338)

악성흑색종세포에서의 향신경요소 수용체 발현 양상

이정열,박영립,황규왕 ( Jung Youl Lee,Young Lip Park,Kyu Uang Whang ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.6

Background: Nerve growth factor(NGF), brain derived neurotrophic factor(BDNF), neurotropin 3(NT-3) and neurotropir-4/5 are neurotrophic factors necessary for the development and maintenance of specific neurors. The tyrosine protein kinase(trk) receptors exhibit specificity for differ ent neurotrophins. NGF is the cognate ligand for the trk A receptor, BDNF binds to trk B receptor and NT-3 binds to irk A, trk B and trk C receptors, Since melanoma cells are devived from neural ectoderm, growth factors which affect. neuronal tissue may have a role in melanoma biology. Objective : The purpose of this study is to demonstrate the presence of trk receptors in rnelanoma cells and observe th effect of K-252a on these melanoma cells growth and differentiation. Methods : After K252a over a range of 0-200nM was added into their cell lines, we exam ined cell viability of SK 28 and SK 30 cells. We performed this to examine the expression of the trk by flow cytometry and immunoblotting. Results : 1. The incubation of . K 28 cells and SK 30 cells with K 252a resulted in a dose dependent inhibition of cell proliferation. 2. In the flowcytometry, SK 28 cells and SK 30 cells showed a high expression of trk A and trk B, not trk C. 3. Using immunoblottiiig, trk in SK 28 cells and SK 30 cells was not expressed. Cpnclusions : These results indicate that. the identification of tyrosine protein kinase reeeptors and their inhibitor which affect differentiation and growth of a melanoma may provide an additional therapeutic option for treatment of melanoma. (Korean J Dermatol 1997;35(6): 1151-1158)

보통 천포창의 세포자멸사 기전에 관한 연구

노병화 ( Byoung Hwa Roh ),황규왕 ( Kyu Uang Whang ),조문균 ( Moon Kyun Cho ),박영립 ( Young Lip Park ),이종석 ( Jong Suk Lee ),이창우 ( Chang Woo Lee ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.7

Background: Pemphigus vulgaris is an autoimmune bullous dermatoses of skin and mucosa characterized by loss of adhesion between keratinocytes, a process known as acantholysis. Apoptosis, programmed cell death, may participate in pathogenesis of intercellular detachment and loss of cell-matrix interaction. Objective: This study was designed to investigate the induction of apoptosis in the pemphigus lesional epidermis and to elucidate the mechanism of apoptosis induced by pemphigus sera. Methods: Hoechst 33342 staining was performed to determine the induction of apoptosis in the pemphigus lesional epidermis. In addition, we used HaCaT cells treated with pemphigus sera and analyzed the expression of caspase-3, caspase-8, caspase-9 and bcl-2, bcl-xL, bax, bak by the RT-PCR method. Results: In Hoechst 33342 staining, typical findings of apoptosis were observed in the pemphigus lesional epidermis showing acantholysis. RT-PCR showed the upregulation of caspase group (caspase-3, caspase-8, caspase-9), the downregulation of antiapoptotic bcl-2 family (bcl-2, bcl-xL) and the upregulation of proapoptotic bcl-2 family (bax, bak). Conclusion: These results suggest that apoptosis may be associated with acantholysis of pemphigus lesional epidermis and may play an important role in the pathogenesis of pemphigus. (Korean J Dermatol 2007;45(7):650∼658)