순무(Brassica rapa L.)의 TNF-α 자극에 의해 유도된 혈관염증 개선 효능

황경아(Kyung-A Hwang),황유진(Yu-Jin Hwang),황혜정(Hye-Jeong Hwang),김영준(Young Jun Kim),최정숙(Jeong-Sook Choe),이성현(Sung-Hyen Lee),장환희(Hwan-Hee Jang) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.2

본 연구에서는 TNF-α에 의해 자극된 혈관세포에서 순무추출물이 염증매개인자 및 세포부착인자에 미치는 영향을 평가함으로써 초기 동맥경화를 예방 및 완화할 수 있는지 평가하였다. 그 결과 HUVECs 세포에 순무 추출물을 처리했을 때 농도 500 μg/mL에서 독성을 나타내어 25, 50, 100μg/mL 농도를 이용하여 혈관 내 염증 억제 효능 실험을 수행하였다. 염증 유발에 의해 유도되는 케모카인(IL-8, MCP-1)과 세포부착인자(ICAM-1, VCAM-1) 유전자와 단백질 발현량은 순무 추출물에 의해 억제되었고, TNF-α 발현 유도 및 IL-8, MCP-1 전사조절에 관여하는 NF-κB의 활성이 순무 추출물에 의해 억제되는 것을 확인하였다. 또한 TNF-α에 의해 감소한 NO 생성량이 순무 추출물에 의해 농도 의존적으로 증가하였고, 혈관 확장 및 NO 생성 효소 eNOS 유전자와 단백질 발현을 증가시켰다. 이상의 연구 결과에서 TNF-α로 유도된 혈관내피세포 염증에 순무 추출물을 처리한 결과 eNOS의 발현 증가에 의해 NO가 증가하고 NF-κB의 활성은 억제시킴으로써 TNF-α에 의해 증가한 IL-8, MCP-1과 ICAM-1, VCAM-1의 발현이 감소하였다. 따라서 본 연구 결과에 의해 순무 추출물은 초기 동맥경화 질환예방 및 개선을 위한 기능성 소재로의 활용 및 건강기능식품으로의 개발 가치를 확인하였다. A high cholesterol diet, tobacco usage, and other negative life style elements are all factors that cause intravascular inflammation by interfering with the flow of blood and damaging vascular tissues. Recent cases of vascular diseases are gradually increasing due to these physical and chemical factors and the vascular inflammation persists, then the early symptoms of arteriosclerosis may occur. Turnip (Brassica rapa L.) is known to be a commonly consumed vegetable that has various physiological properties such as anti-inflammation and anti-cancer activities, among other activities. Therefore, this study investigated the anti-inflammatory effect of turnip extract in tumor necrosis factor-α (TNF-α) stimulated human umbilical vein endothelial cells (HUVECs). The mRNA and protein expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) of the turnip extracts treated group, as compared to that of the TNF-α only treated group, were significantly inhibited at various concentration turnip extract treatment. The RNA of pro-inflammatory chemokines induced by the TNF-α expression were decreased by the turnip extract, and the turnip extract also inhibited NF-κB activity involved in inflammatory signaling. Nitric oxide (NO) was decreased by TNF-α treatment and significantly increased to 10.9 μM in the 100 μg/mL turnip extract treatment groups. Further, the mRNA and protein expressions of endothelial nitric oxide synthase (eNOS) were increased by the turnip extract in a dose-dependent manner. These results suggest the applicability of turnip extracts, because of their anti-inflammatory and vasodilatation functions, could be used to develop functional treatments to prevent the early onset of arteriosclerosis.

무(Raphanus sativus L.)의 활성산소종 생성 억제를 통한 항산화 효능

황경아(Kyung-A Hwang),황혜정(Hye-Jeong Hwang),황유진(Yu Jin Hwang) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.11

본 연구에서는 순무와 월동무의 항산화 효능을 비교 분석하였다. DPPH와 ABTS의 라디칼 소거능 측정 결과, 순무와 월동무 모두 200 ㎍/mL에서 항산화 활성이 가장 우수한 것으로 분석되었다. 그리고 산화 및 염증 유발의 원인인 NO 생성량이 시료 농도 의존적으로 감소하였으며 특히, 순무의 200 ㎍/mL에서 가장 높은 NO 생성 억제율을 나타내었다. 또한, 산화스트레스의 주요 지표 intracelluar ROS와 항산화 효소 유전자 발현 역시 순무 및 월동무 추출물에 의해 조절됨으로써 두 무 추출물이 항산화 활성에 효능이 우수한 것으로 판단된다. 본 연구 결과를 통해 순무 및 월동무 추출물이 항산화 활성이 우수하다는 것을 확인함으로써 이후 항산화 관련 기능성 원료로 활용될 수 있을 것으로 기대되며 항산화 관련 작용기전 규명을 위한 동물실험 등 추가적인 연구를 계속 수행할 예정이다. Radish (Raphanus sativus L.) is a root vegetable native to southeast and central Asia and has been traditionally used in the treatment of constipation, indigestion, and stomach disorders. Radish is classified into various cultivars according to their shape and color. The main ingredients of white radish that are commonly known are flavonoids, arvelexin, and glucosinolate. In addition, red radish contains anthocyanin. These ingredients have been reported to have antidiabetic, anticancer, and antioxidant effects, but no research has yet compared the antioxidant activity of white and red radish. Therefore, in this study, the antioxidant effects of Ganghwa turnip (RG) and Jeju winter radish (RJ) were investigated. Evaluation of DPPH and ABTS radical scavenging activity, the major indicators of antioxidant activity, confirmed significant antioxidant effects at a 200 ㎍/mL concentration of RG and RJ. Also, the production of the inflammatory substance nitric oxide was reduced 55% by RG and 52% by RJ at 200 ㎍/mL. Besides, it was confirmed that the production of reactive oxygen species, a major factor in oxidative stress, was also reduced in a dose-dependent manner. It was further confirmed that the antioxidant enzyme gene expressions such as those of Cu/Zn-superoxide dismutase (SOD), Mn-SOD, glutathione peroxidase, and catalase were increased by RG and RJ extracts. In conclusion, radish has excellent antioxidant function and radish can be developed as a functional food for health in the future.

저온숙성마늘의 라디칼 소거 활성을 통한 산화스트레스 억제 효과

황경아(Kyung-A Hwang),김가람(Ga Ram Kim),황유진(Yu-Jin Hwang),황인국(In-Guk Hwang),송진(Jin Song) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.1

본 연구에서는 마늘의 숙성 기간(15일, 30일, 60일)과 온도(60°C, 70°C)를 달리한 저온숙성마늘과 생마늘의 항산화 효과를 비교 · 분석하였다. DPPH와 ABTS의 라디칼 소거능과 FRAP법에 의한 환원력을 측정한 결과 250 μg/mL에서 생마늘 추출물보다 30일 70°C 추출물과 60일 60°C 추출물의 항산화 활성이 우수하였다. 세포 내 활성산소 생성은 15일 60°C 추출물과 30일 70°C 추출물에서 높은 억제 효과를 보였으며, xanthine oxidase에 대한 활성 저해 효과 역시 15일 60°C 추출물에서 우수하였다. 항산화 효소의 유전자 발현은 LPS를 처리한 군과 생마늘 추출물보다 30일 70°C추출물에서 높은 효과를 보였다. 본 연구 결과를 통해 저온숙성마늘이 생마늘보다 항산화 활성이 우수하다는 것을 확인함으로써 차후 항산화 건강기능식품 소재로서의 활용이 가능할 것으로 판단되나, 저온숙성마늘 추출물의 체내 생리활성 메커니즘 규명을 위해 동물실험 등의 추가적인 연구를 계속적으로 수행할 예정이다. Garlic has drawn attention as a food material for its anti-oxidative and anti-inflammatory properties as well as for prevention and treatment of cancer. In order to increase efficiency, various aging methods for garlic have been attempted. In particular, thermally processed garlic is known to have higher biological activities due to its various chemical changes during heat treatment. Therefore, in this study, we investigated the anti-oxidative effects of garlic extracts aged at low temperature (60∼70°C). In the results, 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis (3-ethylbenzo-thiazoline-6-sulfonate) radical scavenging activities and ferric reducing ability of low temperature-aged garlic (LTAG) were similar to those of raw garlic. LTAG also showed decreased lipopolysaccharide (LPS)-induced production of reactive oxygen species, although there were not significant differences among samples. In addition, xanthine oxidase activity was inhibited by LTAG; the 15 days and 60°C extract showed outstanding inhibition compared with the others. To understand the molecular mechanisms behind the anti-oxidative activity of LTAG, we performed quantitative real-time PCR analysis. The 30 days and 70°C extract upregulated mRNA expression of antioxidant enzymes such as Cu/Zn-superoxide dismutase (SOD), Mn-SOD, glutathione peroxidase, and catalase in LPS-stimulated RAW 264.7 cells. This result indicates that LTAG can be a functional food as a nature antioxidant and antioxidant substance.

참취 추출물과 용매분획물의 항산화 활성

전상민(Sang-Min Jeon),이진영(Jin-Young Lee),김헌웅(Heon-Woong Kim),이영민(Young-Min Lee),장환희(Hwan-Hee Jang),황경아(Kyung-A Hwang),김행란(Haeng-Ran Kim),박동식(Dong-Sik Park) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.9

본 연구는 참취 에탄올 추출물과 용매분획물의 항산화 활성과 총 폴리페놀 함량 및 총 플라보노이드 함량을 측정하고, 생리활성과 항산화 성분 간의 상관관계를 비교?분석하였다. 총 폴리페놀 함량은 70% 에탄올 추출물 99.6 mg GAE eq/g으로 나타났으며, ethyl acetate 분획물 141.9 mg GAE eq/g으로 다른 분획물보다 높은 함량을 나타내었다(p<0.05). 또한 총 플라보노이드 함량도 ethyl acetate 분획물에서 105.6 mg QUE eq/g으로 높은 함량을 나타내었다(p<0.05). DPPH 라디칼 소거능과 ABTS 라디칼 소거능의 경우 ethyl acetate 분획물 1,000 μg/mL 농도에서 97.1% 및 98.7%를 보여 대조군인 ascorbic acid, α-tocopherol 및 BHT와 유사한 소거능을 나타내었다(p<0.05). SOD 유사활성은 ethyl acetate 분획물 1,000 μg/mL 농도에서 42.8%의 활성을 나타내어 DPPH, ABTS 라디칼 소거능보다 비교적 낮은 활성을 나타내었다(p<0.05). FRAP에 의한 환원력 측정 결과에서도 ethyl acetate 분획물에서 높은 활성을 나타내었다(p<0.05). 그러나 Fe<SUP>2+</SUP> chelating 활성 측정은 chloroform 분획물 1,000μg/mL 농도에서 81.8%로 높게 나타났으며, 오히려 ethyl acetate 분획물에서는 6.2%로 가장 낮게 나타났다(p<0.05). 이상의 결과 참취 70% 에탄올 추출물과 용매 분획물 중 ethyl acetate 분획물이 다량의 폴리페놀과 플라보노이드를 함유하고 있으며, 항산화 효과가 높게 나타났다. 따라서 참취는 천연 항산화 소재 및 기능성식품으로의 활용 가능한 약용식물자원인 것으로 판단된다. As an effort to develop functional food ingredients and to discover the biological activity, the total phenolic content, total flavonoid content, DPPH and ABTS radical scavenging activity, SOD-like activity, ferric reducing antioxidant power (FRAP), and Fe<SUP>2+</SUP> chelating of Aster scaber were measured using a 70% ethanol extract and various solvent fractions. As a result, the total phenolic concent was highest in an ethyl acetate fraction of 141.9mg GAE eq/g and the total flavonoid content was 105.6 mg QUE eq/g. The DPPH radical scavenging activity was highest in an ethyl acetate fraction of 97.1% at a concentration of 1,000 μg/mL (p<0.05). The ABTS radical scavenging activity showed a 86.9% ethyl acetate fraction and a 57.9% butanol fraction at a concentration of 125 μg/mL, and higher than that of positive control (α-tocopherol and BHT) (p<0.05). The SOD-like activity showed 42.8% in an ethyl acetate at a concentration of 1,000 μg/mL. The ethyl acetate fraction showed the highest value of FRAP at 1051.9 μM and a concentration of 1,000 μg/mL (p<0.05). The Fe2+chelating was highest in the 70.1% chloroform fraction at a concentration of 500 μg/mL (p<0.05). There is the highest correlation between DPPH radical scavenging activity and FRAP (r=0.981) as compared to other antioxidant assays (p<0.01). With these results, we confirmed that the ethyl acetate fraction of Aster scaber has great antioxidant potential. So it can be expected to be developed into a specific functional food ingredient.

국내 합성피혁제조업 근로자에 대한 디메틸포름아미드의 공기중 농도와 생물학적 노출지표간의 상관성 평가

황양인(Yang In Hwang),이미영(Mi-Young Lee),정윤경(Yun Kyung Chung),김은아(Eun A Kim) 한국분석과학회 2013 분석과학 Vol.26 No.5

면역 관련 건강기능식품(전통식품 소재 중심으로)

황경아(Kyung-A Hwang) 한국식품영양과학회 2020 식품산업과 영양 Vol.25 No.1

식품 및 환경 , 기타 Pseudomonas aeruginosa JRT-4 에 의해 생산된 Biosurfactant 의 계면활성 및 환경적 특성

황경아(Kyung A Hwang),이정래(Jeong Rae Lee),김상종(Sang Jong Kim),김윤석(Yun Seog Kim),안호정(Ho Jeong Ahn) 한국미생물생명공학회 1999 한국미생물·생명공학회지 Vol.27 No.2

N/A

리그난의 화학적 특성, 인체 건강에 미치는 영향 및 리그난의 우유 기능 강화에 관한 연구 고찰

황효정,윤진아,신경옥,Hwang, Hyo-Jeong,Yoon, Jin A,Shin, Kyung-Ok 한국유가공학회 2018 Journal of Dairy Science and Biotechnology (JMSB) Vol.36 No.2

This review discusses the characteristics of major lignans and related studies and provides a basis for future studies. Lignans are present in various food products consumed daily, such as flaxseed and other seeds, vegetables, fruits, and beverages including coffee, tea, and wine. Lignans are natural phytoestrogens with a structure similar to that of secoisolariciresinol (Seco), mataireinol (Mat), pinoresinol (Pin), medioresinol (Med), lariciresinol (Lari), and syringaresinol, which is then converted to enterodiol (END) and enterolactone (ENL), which are mammalian lignans and the primary biologically active enterolignans, by the intestinal microflora. The associations between lignans and a decreased risk of cardiovascular disease are promising; however, they are not yet well-established, probably owing to low lignan intake in habitual Western diets. Nonetheless, these associations were more prominent at the higher doses in interventional studies. Many studies on humans and animals have reported the benefits of lignan consumption in protecting against CVD and metabolic syndrome by reducing lipid and glucose concentrations. END and ENL reportedly exert protective effects including phytoestrogenic, antioxidant, anti-inflammatory, and anticancer effects through various mechanisms. Moreover, lignans reportedly exert beneficial effects in breast, colon, and prostate cancer and osteoporosis have reported that. However, future studies are required to confirm the association between lignan and disease.

연구발표 초록 ( 생물화공 (Ⅱ) ) : 유기용매에서의 효소적 아스파탐 전구체 합성에 관한 연구

황경아 (학),이선복 (정),이경희 (정) ( Kyung A Hwang,Sun Bok Lee,Kyung Hee Lee ) 한국화학공학회 1991 추계학술발표회 Vol.- No.-

건강기능식품 국산 기능성원료(농촌진흥청 연구 중심으로)

황경아(Kyung-A Hwang) 한국식품과학회 2020 식품과학과 산업 Vol.53 No.4

Due to environmental changes such as diet and fine dust, the public has a growing interest in health. With the growing interest in health, consumption of health functional foods to prevent diseases has increased, and the health functional food market is also growing. However, most health functional foods rely on imported raw materials, and the development of health functional foods using domestic raw materials is urgently needed. Therefore, in this paper, provides information on functional raw materials for health functional foods that have been recognized by using domestic agricultural products in Rural Development Administration, and insists that R&D should be more active in order to increase more domestic raw materials in the health functional food market in the future.