모반세포성 모반에서 발생한 악성 흑자 1 예

장대성(Dae Sung Chang),심주현(Joo Hyun Shim),서성준(Seong Jun Seo),송계용(Kye Yong Song),홍창권(Chang Kwun Hong) 대한피부과학회 2001 대한피부과학회지 Vol.39 No.5

Lentigo maligna(LM) is a pigmented lesion that occurs on the sun-exposed skin of an older patients and may become lentigo maligna melanoma(LMM). Currently the percentage of LM type melanoma appears to be increasing, and this rise has been attributed to increasing UV radiation exposure. In addition to UV radiation, estrogen and progesterone, and nonpermanent hair dyes have been proposed as risk factors. A case is a 66-year-old female with a 3 years history of pigmented patch. She had a gradually enlarging irregular bordered brown to black mottled patch on the right temple area. Histologic examination revealed atypical melanocytes along the basal layer of atrophic epidermis, arranged in solitary units and small nests. Perilesional skin showed many scattered melanophages and regressing nevus cell nests which suggested preexisting nevus underwent regressive change. On the basis of clinicopathologic examination we diagnosed this case as lenigo maligna arising from nevocytic nevus and then we removed the lesion by wide surgical excision and could not find any recurrence until now.

가족에서 발생한 신경종성 상피병을 보이는 신경섬유종증 2예

한은실(Eun Sil Han),서성준(Seong Jun Seo),홍창권(Chang Kwun Hong),노병인(Byung In Ro) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.4

We reported two cases of elephantiasis neurofibromatosa in a family. The first case was the son of the second case. Both patients showed huge masses and bone changs. They were treated by surgical excision due t,o functional and cosmetic problems. The histopatholog.c findings of the two cases were consistent with neurofibroma, (Kor J Dermatol 1994; 32(4): 724-723)

안드로겐성 탈모증 환자에서 사이토퓨린(Cytopurine)과 펜타데칸산글리세리드(Pentadecanoic Glyceride) 배합 육모제의 유효성 및 안전성 평가에 관한 임상연구

홍창권 ( Chang Kwun Hong ),한태영 ( Tae Young Han ),강한철 ( Han Chyul Kang ),안승원 ( Seung Won Ahn ),안지영 ( Ji Young Ahn ),서성준 ( Seong Jun Seo ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.12

Background: Androgenetic alopecia (AGA) is the most common type of hair loss found in both males and females. Oral finasteride and topical minoxidil are the only drugs approved, till now, by the Food and Drug Administration of USA for the treatment of this condition. Unfortunately, these treatment options are not satisfactory. Therefore, it has been necessary to develop new agents for this psychologically frustrating disease. Objective: This is a 16-week, double-blinded, placebo-controlled, randomized clinical trial to determine whether a new topical agent, cytopurine/pentadecanoic glyceride topical solution, is effective in showing clinical improvements in patients with AGA. Methods: A total of 74 men and 21 women (24∼55 years old) with AGA were treated either with the new topical solution or a placebo, twice daily. Efficacy was evaluated by daily shed hair count, phototrichogram and patients` subjective and investigators` subjective assessments for clinical improvement. All adverse effects were reported during the study. Results: After 16 weeks of therapy, combination cytopurine/pentadecanoic glyceride topical solution treatment resulted in a significant improvement from the baseline in total hair count and daily shed hair count. But there were no statistically significant differences in anagen hair count, mean hair diameter, patients` subjective and investigators` subjective assessments for clinical improvement and side effects between the cytopurine/pentadecanoic glyceride treated and placebo group. Conclusion: We have confirmed the efficacy of combination cytopurine/pentadecanoic glyceride topical solution in treating AGA, by an objective assessment with phototrichogram. (Korean J Dermatol 2007;45(12):1233∼1239)

Tumor Necrosis Factor - α가 각질형성세포의 Superoxide Radical과 Superoxide Dismutase 활성도에 미치는 영향

홍창권(Chang Kwun Hong),서성준(Seong Jun Seo),노병인(Byung In Ro) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.1

Bsckgaund : Superoxide dismutases(SOD) are important metallo-enzymes which scavenge and dismutase the superoxide free radical. They are thought to be the main enzymes in the antioxidant defense system. In the several cell lines the induction of Mn-SOD expresion by tumor necrosis factor (TNF)-a, which is a known inflammatory cytokine, suggest that Mn-SOD may play a role in the inflammatory process. However, this effect is cell type specific. Objsctive : The present study was undertaken to deterr or whether the induction of SOD expression by TNF-a in the keratinocytes is occurred Methods : Primary keratinocyte cultures from neonatal or skins were grown in complete MCDB 153 medium. Measurement of superoxide radical and SOD activity were performed according to Babicr and Fridovich methods, respectvey. Results : 1. The release of supercxide radical were measured after 30, 60, 90 minutes exposure to 10 ng/ml TNF-a, the results shows 2.94+0.30 nmol/2x10, 3 5+0.52 nmol/2x10, 4.13+0. 70 nmol/2 x10 respectively, The increase of superoxide raduial release was detectable in a time dependent manner. 2. Total SOD activity without treatment of TNF-e at 2 hours was 18.46+2.85. Total SOD activities after treatment of 1, 10, 100 ng/ml TNF-a a 4 hours were 36.47+6.80, 88.01+7.79, 94.50+8.97 respectively, which suggested that TNF-a induced increase of total SOD significantly(P<0.05). TNF-a stimulate total SOD incuction in a dose and time dependent manner, although the effects were not outstanding Mn-SOD activity after treatment of 100 ng/ml TNF a at 48 hours was 84.12+14.83, which was approximately 10.5 fold of that of the untreated case. TNF-a also induced Cu,Zn-SOD activity, but the effect was not so much a in the case of Mn-SOD. Conclusion : TNF-a induces production of total SOD acivities, especially Mn-SOD in the human keratinocytes, which may be to protect cells from, inflammation mediated. oxidative damage, namely prrduction of total SOD protein, esrcally Mn-SOD in response to TNF-a induced and scavrged overproduced superoxide ralic which in turn may result in protection of the keratinocytes. (Kor J Dermatol 1995; 33(1): 19-27)

Dinitrochlorobenzene 면역요법에 의한 소아에서의 사마귀 치료

이주협,홍창권,노병인 ( Joo Hyeup Lee,Chang Kwun Hong,Byung In Ro ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

N/A Immunotherapy of Wart with Dinitrochlorobenzene in Children Joo Hyeup Lee,M.D., Chang Kwun Hong,M.D.,Byung In Ro, M.D. Department of Dermatology, College of Medicine, Chung Ang University Seoul, Korea This study was conducted to evaluate the thrapeutic effect of dinitrochlorobenzene (DNCB) immunotherapy on warts in children. Thirty nine cases of warts were studied in 28 children who visited the Department of Dermatology, College of Medicine, Chung Ang University from January 1989 to June 1990. The type and number of lesions were as follows : periungual warts(17), verruca vulgaris(14), palmoplantar wart(5), and verruca plana juvenilis(3). The jesion were challenged with 0.05% DNCB in weekly intervals after sensitization with 1% DNCB. The results were summarized as follows 1. Sex ratio of patients was 1:1(14 of male, 14 of emale) and mean age was 10.5 year old. 2. Twenty of all 39 cases(51%) were cured. 3. The highest cure rate was achieved for palmoplantar warts(80%), verruca plana juvenilis(67%), verruca vulgaris(50%). The worst outcome occured with periungual wart(41%). 4. Warts of short duration showed higher cure rates than those of long duration. 5. The number of DNCB challenges until cure for the lesion was 5.5 times for palmoplantar wat;5.4 times for periungual wart;5 times for verruca vulgaris;2 times for verruca plana juvenilis. The mean number of DNCB challenge was 4.5. (Kor J Dermatol 29(4) : 500-505, 1991)

원형 탈모증의 임상적 고찰 ( 제 II 보 )

전태진,홍창권,노병인 ( Tae Jin Chun,Chang Kwun Hong,Byung In Ro ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.6

N/A The authors performed a clinical study of 255 patients with εlopecia areata retrospecitively in order to evaluate the clinical manifestation and the effect of treatment with intralesional injection of triamcinolone acetonide suspension from August, 1986 to February, 1991. The results were as follows : 1. The age distribution showed a peak incidence in the third decade(15.5%, 116/255) and average age was 29.6 years. 2. The most common site of involvement was the occipital region(54.1%) in males and the temporal region(51.0%) in females. 3. The effect of treatment in the group treated with intralesional injection of triamcinolone acetonide revealed no significant difference from the group treated with intralesional injection of tramcinolone acetonide and topical application of minoxidil solutior. 4. The area of the bald patch revealed statistically significant correlation with effect of treatment. But the other factors such as sex, age, history of pervious episode, duration and number of lesions were not contributory. These findings suggested that alopecia areata predominantly developed in male and showed excellent therapeutic effect in cases of bald areas less than 5㎠

청피반성 혈관염의 임상 및 병리조직학적 고찰

이창균 ( Chang Kyun Lee ),서성준 ( Seong Jun Seo ),김명남 ( Myeung Nam Kim ),홍창권 ( Chang Kwun Hong ),노병인 ( Byung In Ro ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.4

Background: Livedo vasculitis predominantly affects middle-aged females with relapses in summer, and fluctuating in winter. It develops spontaneously or in association with connective tissue disorders. Histopathologically, the common feature is partial or complete obstruction of vessels with deposits of fibrinoid material in the upper and mid-derrnal vessel walls. Many studies of large series have been reported in the west, but few collective studies of livedo vasculitis have been reported in Korea. Objective : The purpose of this study was to clarify the clinical and histopathologic features of the livedo vasculitis in Korea, Methods : 19 patients with livedo vasculitis who had visited the Department of Dermatolagy, Chung-Ang University Hospital during the last 9 years(1986.1 - 1994.12) were evaluated. Results : 1. The age distribution was in the range of 5-73 years. The prevalence was highest in the third decade. Sex ratio was 1.7: 1.0(12 males : 7 females). 2. All patients had purpuric or telangiectatic lesions on the lower extremities. Painful ulcers developed in 11 patients(78.9%), livedo reticularis in 8(42.1%), and healed leaving whithish atrophic scars in 4 patients(21.1%). 3. In 9 patients(47.4%) the skin lesions developed or aggravated in summer, 6 patients(31.6% ) in winter, 3 patients(15.8%) in spring, and 1 patient(5.3%) in autumn. 4. Leukocytosis was observed in 3 of 17 patients, and VDRL test was positive in 2 of 12 patients. ANA test was positive(1: 40, speckled pattern) in 3 of 7 patients. 5. Histopathologically, partial to complete obstruction of dermal blood vessels with fibrinoid thrombi, endothelial swelling, and extravasation of RBCs were observed in upper and mid-dermis. 6. 16 patients were treated with aspirin, dipyridamole, pentoxifylline and corticosteroid and among them, 10(62.5%) patients were improved slowly, but, 6(37.5%) patients turned out no response or even aggravation. Conclusion : According to this study, seasonal variation of livedo vasculitis showed a tendency tn aggravate in summer and winter. We found that livedo reticularis, which showed slow reduction of blood flow in the subpaillary venous plexus were often followed by livedo vasculitis. (Kor J Dermatol 1996;34(4):572-579)

기저양 모낭 과오종

한은실,서성준,홍창권,송계용,노병인 ( Eun Sil Han,Seong Jun Seo,Chang Kwun Hong,Kye Yong Song,Byung In Ro ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Basaloid follicular hamartoma(BFH) is a benign adnexal tumor with a wide spectrum of clinical appearance and is characterized histologically by the presence of branching cords and thin strands of undifferentiated anasomosing, basaloid proliferations that are embedded in a fibrous stroma. It has been often misdiagnosed and treated as basal cell carcinoma. We report a 57-year-old woman with BFH on the face which is similar to basal cell carcinorna clinically that turned out to be a solitary BFH histologically. There has been no recurrence during one year of follow up after excisional biopsy. (Kor J Dermatol 1997;35(2): 303-306)

우성 이영양성 수포성 표피박리증 2예

이창균,서성준,홍창권,노병인,송계용,이주협 ( Chang Kyun Lee,Seong Jun Seo,Chang Kwun Hong,Byung In Ro,Kye Yong Song,Ju Huep Lee ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.4

Dystrophic epidermolysis bullosa(DEB) is a rare, chronic non-inflamrnatory bullous disease of hereditary trait, which easily produces bullae in response to even minor traurna. We report two cases which developed at birth and infancy, respectively. Bullae, atrophic scars on the knees, ankles and elbows, as well as milia of both hands and loss of nails were seen in both cases. Histologically, cleavage occurs beneath the basal lamina and anchoring fibrils are rudirnentary on electron microscopy. We present two cases of DEB that seem to be dominant types considering the presence of family history with clinical, histological and electronmicroscopic findings. (Kor J Dermatol 1997;35(4): 758-762)

자외선 B를 조사한 각질형성세포의 조건배양액내 Endothelin - 1 이 멜라닌세포의 증식과 기능에 미치는 영향

홍석훈,서성준,홍창권,노병인 ( Seok Hun Hong,Seong Jun Seo,Chang Kwun Haong ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6

Background: The regulatory rnechanisrns of melanoc:ytes underlying ultraviolet(UV) melanogenesis have been an interest tu many investigators. They have shown that several materials produced and secreted frorn norm il human keratinocytes play roles as mitogens of human melanocytes, and demonstrated that UVB exposure stirnulated highly the paracrine linkage of endothelin(ET) be tween keratinocytes and melanocytes. It suggest that ET is one of keratinocytes-derived intrinsic mit.ogens in UVB induced hyperpigmentation. Objective : To evaluate whether ET secreted from keratinocytes under t.he UV irradiation works as paracrine effects such as melanocyte pro)iferation and function, the present, study was under taken. Methpds : Primary kevatinocytes and rnelanocytes cultures from neonatal foreskins were grown in complete MCDB 154 medium. Cultured human keratinocytes were irradiated with UVB 50mJ/ cm2. Twenty fours hour later, conditioned medium of keratinocytes was added into the growth medium of melanocytes of concentration in 10%, 20%, and 30%, respectively. Four days later, in order to detect of melanocytes proliferat.ion and function, number of melanocytes, melanin granules and tyrosinase activity v ere measured. Results : J. The number of me anocytes were higher increase in groups of incubation with conditioned medium of irradiated keratinocytes than that of incubation with conditioned mediurn of irradiated keratinocytes and treatment of anti-ET-1(5ug/ml)(p>0.05). 2. The melanin contents were significant.ly higher increase in groups of incubation with condi t.ioned medium(20%, 30%) of irradiated keratinocyt.es than that of incubation with conditioned medium of irradiated keiatinocytes and treatment of anti-ET-1(5ug/ml)(p<0.05). 3. The tyrosinase acti ity of melanocyte incubated with 30% COllcentration of conditioned medium from cultured keratinocyte irradiated with UVB was significantly higher increase than that of melanocyte incubated with 30% concentration of conditioned mediurn from cultured keratinocytes irradiated with UVB and treated with anti-ET-1(p<0.05). Conclusion : This stud provided an important confirmation of the proposal that ET-1 is intrin sic factor for proliferation and differentiation of human melanocytes. These findings suggest that keratinocyte derived ET-1 make a considerable effect on human melanocyte proliferation and function in UV melanog nesis. (Korean J Dermatol 1997;35(6): 1184-1192)