다양한 계통의 옥수수 미성숙배로부터 캘러스 유도와 식물체 재분화

홍준기,박기진,이강섭,김둘이,김주곤,이승범,서은정,이연희,Hong, Joon Ki,Park, Ki Jin,Lee, Gang-Seob,Kim, Dool Yi,Kim, Ju-Kon,Lee, Seung Bum,Suh, Eun Jung,Lee, Yeon-Hee 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1

옥수수의 최적 조직 배양 조건을 확립하기 위하여 옥수수 국내 5 계통과 국외 11 계통 총 16 계통을 포트와 포장 재배하여 미성숙 배를 분리하여 배발생 캘러스 유도 및 식물체 재분화율을 조사하였다. MS 배지에 auxin으로 1.5 mg/L Dicamba와 0.5 mg/L 2,4-D 가 첨가된 배지에서 캘러스 형성은 본 실험에 사용된 옥수수 계통 모두에서 높은 빈도로 유도되었으며, 캘러스로부터 식물체 재분화는 5mg/L zeatin이 첨가된 재분화 배지에서 높은 재분화율을 보였다. 또한 포장에서 재배된 옥수수로부터 미성숙 배를 분리하여 사용하였을 때 캘러스 유기 및 식물체 재분화 효율이 높았던 것으로 보아 미성숙 배를 분리하기 위한 옥수수 상태 및 genotype이 중요한 영향을 준다는 것을 알 수 있었다. 본 실험을 통하여 배 발생 캘러스 형성 및 식물체 재분화 효율이 조사된 옥수수 계통들은 생명공학 기술을 활용한 신품종 개발을 위한 형질전환 시스템 개발에 유전자원으로 활용될 수 있는 정보를 제공할 것으로 사료된다. We investigated the callus induction and plant regeneration ability of 16 maize genotypes, including the Korean inbred lines, using 9 to 15 day-old immature zygotic embryos from maize grown in pots and from field cultures. Immature zygotic embryos placed on MS medium supplemented with L-proline 0.7 g/L, MES 0.5 g/L, Dicamba 1.5 mg/L, 2,4-D 0.5 mg/L, $AgNO_3$ 4 mg/L, and sucrose 20 g/L, showed the highest frequency of callus induction. The highest number of shoots regenerated when the embryogenic callus were transferred to MS medium supplemented with 5 mg/L zeatin. The root formation was observed when shoots were grown on MS medium supplemented with 0.2 mg/L indole-3-butyric acid (IBA). Additionally, under the same culture conditions, immature zygotic embryos from maize grown in the field also had a high frequency of plant regeneration. Except one genotype, 15 genotypes showed callus induction and shoot regeneration. Among the 16 genotypes tested, H99, B98, HW3, and B73 yielded the best plant regeneration. H99 showed maximum shoot formation from the primary embryogenic callus. The results suggest that genotypes and growth conditions of the maize plant plays very important roles for enhancing the embryogenesis competence of immature zygotic embryos. The successful regeneration from immature zygotic embryos of maize inbred lines provides a basis for molecular breeding of new cultivars by genetic transformation.

운동성 장애에 대한 감마나이프 시술의 초기경험

홍준기,김무성,이선일,정용태,김수천,심재홍,Hong, Joon Ki,Kim, Moo Seong,Lee, Sun Il,Jung, Yong Tae,Kim, Soo Chun,Sim, Jae Hong 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.8

Objectives : With recent improvements in neuroimaging and the development of third and fourth-generation radiosurgical dose-planning soft ware, came a renewed interest in using radiosurgery for the treatment of movement disorders. Radiosurgery involves no opening of the cranium and no incisions, eliminating both the risk of hemorrhage from passing an electrode to the depths of meningitis from operative infection. It is for these reasons stereotactic radiosurgical treatment of movement disorders has value in a small subgroup of patients. The authors report four cases of Parkinson's disease and one case of dystonia that were treated by Gamma knife. Methods : Radiosurgical nucleus ventralis intermedius thalamotomy using the gamma knife unit was performed to make lesion in two Parkinson's disease patients. A radiation dose of 120Gy was delivered to nucleus using a single 4-mm collimator plug pattern following classic anatomical landmarks. Patients were followed for a median of 10.5 months(range 9-12 months). An independent neurological evaluation of tremor, based on the change in the United Parkinson's Disease Rating Scale tremor score(UPDRS), was correlated with a subjective evaluation. Gamma knife ventrolateral(V.O.P) thalamotomy was performed in one case of dystonia. A central dose of 150Gy was delivered and the patient was followed for 18 months. Gamma knife globus pallidus interna pallidotomy was performed in two Parkinson's disease patients. A radiation dose of 130Gy(range 120-140Gy) was delivered. Patients were followed for a median of 13 months(range 9-14 months). Result : Ventrolateral thalamotomy in dystonia produced regained left hand usage in order to be able to use the telephone. Ventralis intermedius thalamotomy produced an excellent improvement of the tremor in one case, mild improvement of the tremor in the other case of Parkinson's disease. A globus pallidus internalis(GPi) pallidotomy produced improvement of rigidity and dyskinesia : one other showed no change. There were no neurological complications. Conclusion : Gamma Knife thalamotomy considered a safe and effective technique for the treatment of tremor in Parkinson's disease. Although the results from Longer follow-up is not available yet, the short-term results seem to be encouraging.

Prostaglandin $F2{\alpha}$ 투여가 수퇘지의 성행동과 정액 채취 훈련에 미치는 영향

홍준기,유재원,조규호,김명직,박준철,김인철,정일병,Hong, Joon-Ki,Ryu, Jae-Weon,Cho, Kyu-Ho,Kim, Myung-Jick,Park, Jun-Chul,Kim, In-Cheul,Jung, Il-Byung 한국수정란이식학회 2009 한국동물생명공학회지 Vol.24 No.1

Prostaglandin $F_2{\alpha}$ ($PGF_2{\alpha}$) can facilitate release of epinephrine from the adrenal gland. The objective was to extend these findings and determine the effects of $PGF_2{\alpha}$ on sexual activity and semen collection training in sexually inexperienced boars. Boars (n=32; $281{\pm}18$ days of age) were moved individually once weekly to a semen collection room equipped with an artificial sow. Before entering the semen collection room, boar received i.m. treatments of $PGF_2{\alpha}$ at doses of 5 (n=8), 10 (n=8), or 20 (n=8), and control boar (n=8) were not treated. Reaction time (elapsed time after entering collection pen until the start of mounting) for boars receiving 5mg ($3.3{\pm}0.9\;min$), 10mg ($3.3{\pm}0.8\;min$) $PGF_2{\alpha}$ was shorter (p<0.05) than for controls ($6.7{\pm}0.9min$). Duration of ejaculation (min) per session was longer (p<0.05) for $PGF_2{\alpha}$ (10 mg, 20 mg)-treated boars ($7.3{\pm}0.7\;min$, $6.9{\pm}0.7\;min$), compared to control ($3.4{\pm}0.8\;min$). The number of training session per boars was less (p=0.056) for $PGF_2{\alpha}$ 10mg-treated boars ($1.0{\pm}0.4$), compared to control ($2.0{\pm}0.4$). Semen characteristic such as volume, concentration, the number of total ejaculated sperm, were similar for $PGF_2{\alpha}$-treated and controls. There was no apparent difference on sperm movement characteristics (Mot: motility, VCL : curve linear velocity, VSL : straight line velocity, VAP : average path velocity, LIN : linearity) after semen preservation by collected with or without $PGF_2{\alpha}$ treatment. In summary, administration of $PGF_2{\alpha}$ in boars increased the sexual activity and facilitated the training boars to mount an artificial sow for semen collection, but did not affect semen characteristic.

단위가축영양 : 육계에서 구멍갈파래의 항산화 및 면역조절 효과

홍준기 ( Joon Ki Hong ),봉미희 ( Mi Hee Bong ),박준철 ( Jun Cheol Park ),문홍길 ( Hong Kil Moon ),김동욱 ( Dong Wook Kim ),이상철 ( Sang Cheul Lee ),이준헌 ( Jun Heon Lee ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.5

This study was conducted to test the efficacy of Ulva pertusa kjellman as immunomodulators under lipopolysaccharide(LPS) challenge in broilers by investigating their effects on serum superoxide dismutase(SOD) like ability, immunoglobulin concentration, and splenic cytokine mRNA expression. A total of ninety six1-d-old male broiler chicks(Ross 308) were divided into 4 treatment groups with 8 replicates of 3 birds in each group. NC(negative control, no immune substances), PC(positive control, β-glucan 25 ppm), Ulva P(Ulva pertusa kjellman Powder 3%), and Ulva E(Extract form Ulva pertusa kjellman 0.3%) were added in feed with respective substance. Heparinized venous blood and spleens were collected from five birds per dietary treatment at 5 wk of age. The SOD like activities in Ulva P and Ulva E were significantly increased in comparison with other groups(P<0.05). The immunoglobulin M content was lower in the Ulva E than other groups(P<0.05). Expression patterns of splenic cytokine mRNA in the IL-1β, IL-2 and IL-6 were similar. Expression rate of IL-1β, IL-2 and IL-6 in Ulva pertusa kjellman(Ulva P, Ulva E) were decreased(P<0.05) in comparison to other groups. Expression rate of IL-1β, IL-2 and IL-6 were significantly lower in groups treated with Ulva E than Ulva P. In conclusion, dietary supplementation of Ulva pertusa kjellman improved SOD like activity and affect immune system by inhibiting inflammatory response in broiler chicks. In addition, it is more effective to use Ulva pertusa kjellman extract than powder for immunomodulatory function. These results suggest the possibility that Ulva pertusa kjellman could be used as the immunomodulator for inflammatory response in broiler chicks.

Expression of a Chinese Cabbage Cysteine Proteinase Inhibitor, BCPI-1, Retards Seed Germination and Early Seedling Growth Rate

Joon Ki Hong(홍준기),Eun Young Lee(이은영),Zheng Lu Jin(김정률),Kyung Ae Yang(양경애),Young Ju Choi(최영주),Woo Sik Chung(정우식),Ho Il Kim(김호일),Dae-Jin Yun(윤대진),Sang Yeol Lee(이상열),Moo Je Cho(조무제),Chae Oh Lim(임채오) 한국원예학회 2003 Horticulture, Environment, and Biotechnology Vol.44 No.5

홍삼 부산물이 스트레스에 대한 산란계 생체반응에 미치는 영향

홍준기(Joon Ki Hong),봉미희(Mi Hee Bong),박준철(Jun Cheol Park),문홍길(Hong Kil Moon),이상철(Sang Cheul Lee),이준헌(Jun Heon Lee),황성구(Seong Gu Hwang) 韓國家禽學會 2012 韓國家禽學會誌 Vol.39 No.1

This study was conducted to determine the possible use of Red Ginseng marc as stress inhibiter in thermal stress (temperature humidity index 86) and lipopolysaccharide (LPS)-exposed laying hens by investigating their effects on laying performance, blood biochemical parameters, immunoglobulin concentration and serum superoxide dismutase (SOD) like ability. A total of forty-five 52-wk-old laying hens (ISA Brown) were divided into 3 treatment groups with 5 replicates of 3 birds in each group. NC (negative control, no immune substances), PC (positive control, β-glucan 25 ppm) and RGM (Red Ginseng Marc 3%) were added in feed with respective substance. Egg production in RGM was significantly increased in comparison with NC groups for 8 weeks (P<0.05). On blood biochemical parameters, effects of ambient temperature is definite by showing significant difference in aspartate aminotransferase and others (P<0.05), but RGM both before and after thermal stimulation have no significant difference in comparison with other groups. And for 3 weeks after thermal stimulation, laying performance was also not significantly different among treatments. Immunoglobulin M content and SOD like activities after challenge with LPS were higher in the RGM and PC than NC (P<0.05). In conclusion, although ineffective as inhibiter in thermal stress, dietary supplementation of Red Ginseng marc improved SOD like activity and immune system by regulating immunoglobulin content in laying hens. These findings have laid the foundation for future studies of immunomodulation in laying hens fed Red Ginseng Marc and of evaluation of heat stress inhibitor.

유한요소해석 프로그램을 이용한 F-4D 전투기의 콘크리트 벽체 충돌 해석

홍준기(Hong, Joon-Ki),강현구(Kang, Thomas H.-K.) 한국콘크리트학회 2017 한국콘크리트학회 학술대회 논문집 Vol.29 No.1

국내 두록 품종의 근교수준 및 유효집단 크기 추정

홍준기(Joon Ki Hong),송나래(Na Rae Song),김두완(Du Wan Kim),김시동(Si Dong Kim),김영화(Young Hwa Kim),최재관(Jae Gwan Choi),문효식(Hyo Sik Mun),조규호(Kyu Ho Cho) 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.1

본 연구는 국내 주요 두록 종돈장의 혈통자료를 이용하여 근교수준과 유효집단 크기를 추정하기 위해 수행하였다. 혈연관계를 가지기 시작한 1987년부터 2013년까지 총 86,639두의 혈통자료를 최종적으로 이용하여 6개 종돈장의 세대별 근교계수 및 유효집단크기를 추정하였다. 종돈장별 세대에 따른 근교계수 변화는 2가지 패턴으로 조사되었으며, 근교계수 평균은 5% 이내로 적절한 수준을 유지하는 것으로 사료된다. 유효집단 크기는 종돈장별 다양한 차이를 보였지만 모든 종돈장이 최소권장수준인 50두 이상으로 유지하는 것으로 분석되었다. 대부분의 종돈장이 낮은 근교수준을 유지하는 것으로 조사되었는데, 근교계수를 고려한 교배관리와 외부 종돈 도입이 주된 원인이라 사료된다. 외부 종돈을 수입해서 도입하는 경우, 집단의 유전적 다양성은 증가할 수 있지만, 향후 국제적 종자주권 행사에는 불리한 상황이 될 수 있다. 특히 나고야의정서의 ABS (Access, Benefit, Sharing) 규정(2010)에 따라 유전자원을 제공하는 업체나 국가가 종자 주권 행사에 유리하도록 전개되고 있다. 그리고 업체상호 간 계약합의가 국제거래규약의 효력을 가지기 때문에 정액, 자손 등 종자의 사후활용에 대해서도 로열티를 요구할 수 있다. 따라서 유효집단크기를 높이기 위한 방법으로 무분별한 수입보다는 국내종돈장 사이에서 우수 종돈을 공유하는 것이 필요할 것으로 사료된다. The pedigree data of 86,639 heads from six major swine breeding farms were investigated to check levels of inbreeding and effective population size of breeding stocks. The average rate of inbreeding was 1.04%, 0.87%, 3.17%, 1.05%, 3.23% and 3.33% for farms A, B, C, D, E and F, respectively. The average inbreeding rate was highest for F farm and lowest for B farm. In farms D and E, there were quite large numbers of immigrant animals per generation compared to other farms. The effective population sizes calculated from the average rate of inbreeding were distributed between 83.0 and 814.8. Specific values were 282.3, 225.5, 83.0, 814.8, 302.9 and 175.7 for farms A, B, C, D, E and F, respectively. The results showed that there was no cause for concern over the current inbreeding level of major swine breeding farm populations and the inbreeding level was within an acceptable range. In addition, internal sharing rather than importing pig is necessary to strengthen seed sovereignty.

배추 속 작물의 개화형 판별 마커 시스템 개발

김진아,김정선,홍준기,이연희,이수인,정미정,Kim, Jin A,Kim, Jung Sun,Hong, Joon Ki,Lee, Yeon-Hee,Lee, Soo In,Jeong, Mi-Jeong 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.4

개화는 배추종 작물의 생산성과 연관된 중요 발달 특성 중 하나이다. 이식 후, 갑작스러운 저온에 노출되어 때이른 개화를 하게 되면 수확되는 생산물의 양과 질이 떨어지게 된다. 따라서, 개화조절 메커니즘을 이해하는 것은 배추 종 작물의 농업적 생산성을 향상시키는데 도움을 줄 것이다. 춘화는 배추과 작물에서 일반적으로 알려져 있는 개화를 유도하는 중요한 요소이다. 그러나 옐로우 사순이나 코마수나와 같은 배추 아종은 춘화처리 없이도 개화한다. 1일을 주기로 하여 생물의 생리기작을 조절하는 생체시계 유전자는 일장감응형의 개화 조절에 중요한 역할을 하지만 춘화처리를 통해 개화를 유도하는 기작과도 연관되어 있다. 본 논문에서는 22개의 배추 아종을 개화에 춘화처리가 필요한 춘화형과 춘화처리 없이도 개화하는 비춘화형으로 나누어 보존된 생체시계 유전자, BrPRR1 군의 염기서열 분석을 수행하였다. 그 중 BrPRR1b 유전자의 결손 영역으로 춘화형과 비춘화형 두 그룹을 구분할 수 있었다. 이 서열변이를 증폭할 수 있는 PCR 프라이머를 디자인하여 비춘화형 배추 아종에서는 451 bp의 긴밴드를, 춘화형 배추에서는 422 bp의 작은 크기의 밴드를 증폭할 수 있었다. 이 프라이머 세트는 43개 배추 아종과 4개의 배추속 작물, 브로콜리, 양배추, 갓, 그리고 유채의 개화형을 구분하는데 적용되었다. 각 작물의 PCR 결과와 개화시기에 대한 정보를 통하여 프라이머 세트가 개화형을 판별할 수 있는 마커로 이용될 수 있음이 확인되었다. 이 마커시스템은 배추 종 작물 육종에 유묘 단계에서 개화형을 판단하는데 이용할 수 을 것이다. 또한 이 결과들은 생체시계 유전자가 배추 종 작물의 개화를 조절하는 좋은 전략이 될 수 있음을 보여주었다. Flowering is one of the most important development traits related to the production of Brassica rapa crops. After planting, a sudden low temperature triggers premature flowering, which leads to a reduction in the yield and quality of harvested production. Therefore, understanding the mechanism of flowering control is important in the agricultural productivity for preventing Brassica rapa crops. Vernalization is generally known as the main factor of flowering in the Brassica plant. However, in the subspecies of Brassica rapa, some accession such as Yellow sarson and Komatsuna display the flowering phenotype without vernalization. Circadian genes, which diurnally regulate plant physiology, have a role for photoperiodic flowering but are related to the regulation of the vernalizarion mechanism. In this report, the 22 B. rapa accession were divided into two groups, vernalization and non-vernalization, and the sequenced circadian gene, BrPRR1s. Among them, the BrPRR1b gene was found to have deletion regions, which could classify the two groups. The PCR primer was designed to amplify a short band of 422bp in the vernalization type and a long band of 451bp in the non-vernalization type. This primer set was applied to distinguish the flowering types in the 43 B. rapa accession and 4 Brassica genus crop, Broccoli, cabbage, mustard, and rape. The PCR analysis results and flowering time information of each crop demonstrated that the primer set can be used as marker to discern the flowering type in Brassica crops. This marker system can be applied to the B. rapa breeding when selecting the flowering character of new progenies or introducing varieties at an early stage. In addition, these results displayed that the circadian clock genes can be a good strategy for the flowering control of B. rapa crops.

돼지 주요 경제형질들에 영향을 미치는 유전 및 환경효과 추정

이일주(Ill-Joo Lee),홍준기(Joon-Ki Hong),김두완(Du-Wan Kim),사수진(Soo-Jin Sa),김영화(Young-Hwa Kim),조규호(Kyu-Ho Cho) 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.4

본 연구는 국립축산과학원에서 사육하고 있는 듀록, 랜드레이스, 요크셔 품종 300두의 검정기록 자료를 이용하여 성별, 품종별, 검정종료일령별 효과를 추정하기 위하여 수행하였다. 검정 종료 체중과 측정 mode별 도체 형질의 품종별 차이는 등지방 두께에 있어 종료체중 90 ㎏시 A-mode, 90 ㎏시 B-mode 및 110 ㎏시 B-mode의 세 경우에 있어 A-mode 보다는 B-mode가, 90 kg 종료시 보다는 110 ㎏ 종료시가 더 높은 등지방두께 추정치를 나타내었다. 등심단면적의 경우는 90 ㎏인 경우와 110 ㎏인 경우로 구분하여 측정하였는데, 90 ㎏ 종료시 보다는 110 ㎏ 종료시가 더 높은 등심단면적 추정치를 나타내었고, 품종별로는 Duroc종이 유의적으로 넓었다(P<0.05). 성별의 효과를 살펴보면, 도체 형질에 있어서 등지방 두께는 수컷이 얇았으나, 등심 단면적은 암컷이 더 넓었다. 또한 분석모델에서 공변이로 포함한 모델에서 90 ㎏시 A-mode 등지방 두께, 110 ㎏시 B-mode 등지방 두께, 90 ㎏시 B-mode 등심단면적에서 검정종료일령의 효과가 유의하게 작용하였다. 향후 국가 단위 개량체계가 확립되면 농장검정을 통한 도체형질에 대한 정확한 육종가 추정을 위해서 측정장비의 개선과 측정 요원의 훈련을 통한 정확한 측정이 우선되어야 할 것으로 사료된다. This study was conducted to estimate the effect of sex, breed and on the basis of the records on 300 pigs of Duroc, Landrace and Yorkshire breeds raised at the National Institute of Animal Science. Yorkshire was observed to be superior to other breeds between 90 to 110 ㎏ of body weight. Thicker backfat was estimated in the B-mode than in A-mode of 110 ㎏s range than in 90 ㎏s. Loin eye muscle area was larger in the final weight of 110 ㎏ than in 90 and the Duroc had significantly (p<0.05) larger loin eye muscle area than other breeds. Thinner backfat was observed in males than in females in both modes of backfat measurements and in both of the final weights of test. Females had significantly larger loin eye muscle area than males, and the difference between the two sexes were larger in the final weight of 110 ㎏ than in 90 ㎏. Analysis models concluded that backfat thickness certainly influences the body weight gain when at 90 ㎏s and 110 ㎏s analysed with A-mode and B-mode respectively.