LC-MS/MS를 이용한 인체시료 중 프탈레이트 대사체 동시분석법 확립

홍순근,남혜선,정기경,강일현,김태성,조상은,정수희,이장우,김준철,고영림,강태석,Hong, Soon-Keun,Nam, Hye-Seon,Jung, Ki-Kyung,Kang, Il-Hyun,Kim, Tae-Sung,Cho, Sang-Eun,Jung, Su-Hee,Lee, Jang-Woo,Kim, Jun-Cheol,Kho, Young-Lim,Kang, Tae-Se 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.6

Phthalates, such as di (2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) have been proved to be teratogenics and endocrine disruptors, metabolized rapidly and excreted in the urine. In this study, a simultaneous analytical method for 10 phthalate metabolites, MnBP, MiBP, MBzP, MCHP, MEHP, MEHHP, MEOHP, MnOP, MiNP and MiDP, in human urines, based on switching system with on-line pretreatment column using HPLC-MS/MS has been developed. This method was validated according to the guideline of bioanalytical method validation of National Institute of Toxicological Research. Limits of detection range between 0.2 and 0.9 ng/ml for 10 phthalate metabolites. The calibration curves showed linearity in the range 0.997~0.999, and the results of the intra- and inter-day validations were in the range from 0.4 to 14.7% RSD and from 0.3 to 9.4% RSD, respectively. Recoveries of phthalate metabolites varied from 87.0 to 116.1%. This analytical method showed high accuracy and stable precision for all metabolites, and seems to be suitable for biomonitoring of phthalates in human urine.

Studies on the Sfety of Korean Ginseng Ingested as Food Substance

홍순근,윤한교,장규섭,Hong, Soon Keun,Yoon, Han Kyo,Chang, Kyu Seob Institute of Agricultural Science 1984 農業技術硏究報告 Vol.11 No.2

In order to study the safety of ginseng ingested as a food substance, rats were fed ginseng added feed (subgroup 1: 0.625g/kg feed; subgroup 2: 1.25g/kg feed; subgroup 3: 2.5g/kg feed) for periods up to 6 months. Growth rates, blood composition, blood cell counts, and histopathological studies were carried out on both the control and test animals to study the effect of ingested ginseng. Following results were obtained: 1. The growth rates of the test and control animals were similar with the exception of the subgroup 3 in the 2-month fed animals who had a lower weight gain, and the subgroup 2 in the 4-month fed group who had a higher weight gain than the controls. 2. Liver, spleen, kidney, brain, lung and heart weights were similar between the test and the controls. 3. RBC, WBC, hematocrit, and hemoglobin values were similar between the test and control animals. the blood composition determination showed a similar level of alkaline phosphatase, triglyceride, total cholesterol, S-GOT, free cholesterol between the test and controls. However, free fatty acid, total lipid and S-GPT levels decreased while phospholipid, total protein, and HDL-cholesterol levels tended to increase. 4. Fl, F2 generations showed no abnormai values in blood count, organ weight and external appearance. 5. No histopathological differences were observed between the test and control animals even after a long-term administration of ginseng. However, there was evidence that ginseng promotes hematopoiesis in test animals. 6. These results suggest that ginseng is not only safe as a food substance but may indeed strengthen the body and help-prevent diseases of old age.

Studies on the Sfety of Korean Ginseng Ingested as Food Substance

Soon Keun Hong(洪淳根),Han Kyo Yoon(尹漢敎),Kyu Seob Chang(張奎燮) 충남대학교 농업과학연구소 1984 農業技術硏究報告 Vol.11 No.2

인삼 사포닌의 High Performance Liquid Chromatography에 의한 분리

홍순근(Soon Keun Hong),박은규(Eun Kyue Park),이춘영(Choon Young Lee),김명운(Myong Un Kim) 대한약학회 1979 약학회지 Vol.23 No.3,4

A high performance liquid chromatograpic procedure is described for determining ginseng saponins such as ginsenoside-Rb1, -Rb2, -Rc, -Rd, -Re, -Rf, -Rg1, and-Rg2. Ginseng saponins extracted with 90% methanol and water-saturated butanol were compared with pure standard ginsenosides. The resolution of the saponins was satisfactory and detection limit for each saponin was about 5mcg. Separation of the saponins was accomplished using a mc Bondapak carbohydrate analysis column, mobile phase of acetonitrile-water-butanol (80:20:15) and differential refractive index(RI) detector. The reproducibility and the recovery were also studied. This method was applied for determining the saponin contents of several parts of leaf, fresh ginseng, white ginseng, and red ginseng.

인삼(人蔘) 각(各) Fraction이 Nembutal, Chlorpromazine 및 Reserpine 투여(投與)로 인(因)한 Mouse체온하강(體溫下降)에 미치는 영향(影響)

홍사악(Hong, Sa-Ack),조항영(Cho, Hang-Young),홍순근(Hong, Soon-Keun) 대한약리학회 1969 대한약리학잡지 Vol.5 No.2

Saponin, essential oil, fat oil and alkaloidal fraction were fractionated from the ethanol extract of fanax ginseng. Effect of each fraction of Panax ginseng upon the temperature response induced by reserpine, chlorpromazine and nembutal was investigated in mice, so as to secure some hidden facets of each fraction of Panax ginseng acting upon central nervous system. The authors could arrive at some results, that is: (1) Inhibitory effect of Panax ginseng upon temperature decline induced by nembutal and chlorpromazine and potentiating action upon hyperthermia induced by reserpine reside mainly in saponin fraction and slightly in essential oil fraction. (2) The effect of Ginseng saponin on temperature response in nice seems to be related with the liberation of serotonin and histamine.

고속액체(高速液體) Chromatography에 의(依)한 $Ginsenoside-Rf,\;Rg_2$ 및 $Rh_1$의 분리(分離)

최진호,김우정,홍순근,오성기,대포언길,Choi, Jin-Ho,Kim, Woo-Jung,Hong, Soon-Keun,Oh, Sung-Ki,Oura, Hikokichi 한국응용생명화학회 1980 Applied Biological Chemistry (Appl Biol Chem) Vol.23 No.4

인삼(人蔘)사포닌중 소미량성분인 $ginsenoside-Rf,\;-Rg_2$ 및 $-Rh_1$을 조제용(造製用), 준조제용(準造製用) 및 분석용(分析用) HPLC를 사용(使用)하여 분리(分離)하였다. 본(本) 방법(方法)은 신속하며 이들 소미량 ginsenoside의 분리(分離) 및 동정(同定)에 매우 유효(有效)하였으며 재(再)순환 방식을 사용하여 달성되었다. The minor components of $saponin-ginsenoside-Rf,\;-Rg_2\;and\;-Rh_1$ were isolated from Panax ginseng C.A. Meyer by preparative, semi-preparative and analtical high performance liquid chromatography. The rapid method developed in this work was proved to be very effective in separation and isolation of these minor ginsenosides. A further isolation was achieved by using the recycling technique.

β - D - galactosidase 불활성화에 대한 동력학적 연구

김두하,한영희,홍순근 ( Doo Ha Kim,Young Hee Hahn,Soon Keun Hong ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.1

An improved method of determining the rate constant of an enzyme inactivation was successfully applied to the kinetic study of thermal inactivation of β-D-galactosidase (EC. 3.2.1.23) concerning the hydrogen ion concentration and the initial concentration of the enzyme. The rate constant and halflife of the enzyme could be determined conveniently from the recorded data of absorbance during inactivation by the asymptotic regression method when the substrate depletion could be neglected. The rate constant of inactivation was dependent on the ligand molecules such as metal cofactors, substrate concentration and proton concentration etc. Under the assumption of equilibrium state between each complexed form of different inactivation rate constant, kinetic equations of the apparant rate constant could be derived and applied to the H^+ concentration. The rate constant of inactivation increased with a double sigmoid pattern with decreasing H^+ concentration. And it could be explained by assuming 4 intermediates of ionized enzyme forms. This means that 3 different ionizable side chains of the enzyme contribute largely to the conformational stability of this enzyme. The reaction order of inactivation of β-D-galactosidase increased continuously with increasing the enzyme concentration, and it was affected by the inactivation temperature. In this work, it increased from 1 to about 2. The apparant rate constant increased sharply with decreasing the enzyme concentration below a critical value but it increased slowly with increasing the enzyme concentration above the critical point. This could be successfully explained by a dimerization model and trimerization model as suggested in this work.

Inactivation Kinetics of ${\beta}$-D-galactosidase

김두하,한영희,홍순근,Kim, Doo-Ha,Hahn, Young-Hee,Hong, Soon-Keun 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.1

효소의 불활성화 속도상수를 결정하는 새로운 측정방법을 고안하였으며, ${\beta}$-D-galactosidase (EC. 3.2.1.23)의 열변성에 있어서 수소이온 농도 및 초기효소농도 변화에 따른 효소불활성화의 작용기전을 규명하는데 이 방법을 적용하였다. 효소 불활성화 속도상수와 효소활성의 반감기는 효소반응을 진행시키면서 열에 의하여 불활성화를 유도할때 나타나는 흡광도의 증가를 연속적으로 기록 한 다음, 이동안 기질농도의 변화가 무시될 수 있을때는 점근회기방법에 의하여 이효소의 불활성화 속도상수를 결정할 수 있었다. 효소분자끼리 회합하지 않고, 소분자 물질, 예를들면 금속이온, 기질 및 수소이온등이 효소분자와 결합하여 이들이 평형상태를 이루고 각 결합형들이 서로 다른 반응속도로 불활성화 될 때는 불활성화 속도상수는 효소농도에는 무관하고 단지 결합하는 소분자들에 의해서만 영향을 받음을 수소이온의 경우를 통하여 확인하였다. 이 효소의 불활성화 속도상수는 반응액의 수소이온 농도가 감소함에 따라서 계단식으로 증가함이 관찰되었으며, 이것은 4가지 형태의 이온화한 효소의 중간체를 가정하면 이론적으로 설명할 수 있었다. 따라서 서로 다른 3가지 종류의 아마노산 측쇄가 이효소의 안정성에 크게 관여한다는 것을 알 수 있었다. 이효소의 불활성화 반응차수는 효소의 농도가 증가함에 따라서 연속적으로 증가하였으며, 효소농도에 따른 불활성화 반응차수는 온도에 크게 영향을 받음을 알 수 있었다. 본연구의 실험조건에서는 반응차수가 1에서 2까지 증가하였다. 이때 겉보기 불활성화 반응속도상수는 효소의 어떤 농도를 기준으로 하여 그보다 묽어질때는 급격히 증가하였으며 그보다 진한 농도에서는 완만하게 증가함을 보여주었다. 이현상은 효소의 2분자 또는 3분자 간의 회합을 가정하면 설명이 가능하였다. An improved method of determining the rate constant of an enzyme inactivation was successfully applied to the kinetic study of thermal inactivation of ${\beta}$-D-galactosidase (EC. 3.2.1.23) concerning the hydrogen ion concentration and the initial concentration of the enzyme. The rate constant and halflife of the enzyme could be determined conveniently from the recorded data of absorbance during inactivation by the asymptotic regression method when the substrate depletion could be neglected. The rate constant of inactivation was dependent on the ligand molecules such as metal cofactors, substrate concentration and proton concentration etc. Under the assumption of equilibrium state between each complexed form of different inactivation rate constant, kinetic equations of the apparant rate constant could be derived and applied to the $H^+$ concentration. The rate constant of inactivation increased with a double sigmoid pattern with decreasing $H^+$ concentration. And it could be explained by assuming 4 intermediates of ionized enzyme forms. This means that 3 different ionizable side chains of the enzyme contribute largely to the conformational stability of this enzyme. The reaction order of inactivation of ${\beta}$-D-galactosidase increased continuously with increasing the enzyme concentration, and it was affected by the inactivation temperature. In this work, it increased from 1 to about 2. The apparant rate constant increased sharply with decreasing the enzyme concentration below a critical value but it increased slowly with increasing the enzyme concentration above the critical point. This could be successfully explained by a dimerization model and trimerization model as suggested in this work.

흰쥐의 혈청 콜레스테롤 및 중성지방 수준에 미치는 인삼 총 사포닌의 영향

임창진,박은희,이동원,이송재,홍순근 ( Chang Jin Lim,Eun Hee Park,Dong Kwon Rhee,Song Jae Lee,Soon Keun Hong ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.3

Effects of administration route and dose of ginseng total saponin on the levels of serum cholesterol and triglyceride in normal and cholesterol-administered rats were studied. The results are as follows 1. In normal rats, serum cholesterol was decreased in proportion to the administered dose of purified total saponin. 2. In high-cholesterol administered rats, administration of total saponin 15 ㎎/㎏ (p. o.) increased serum cholesterol, but total saponin 150 ㎎/㎏ decreased it. And it was also observed that administration of prosapogenin 50 ㎎/㎏ had about the same effect as oral administration of total saponin 15 ㎎/㎏. 3. Serum triglyceride was slightly decreased by administration of total saponin, but there was no correlationship between administered total saponin dose and serum triglyceride level.

人蔘屬과 吾加皮屬의 Nucleobases 및 Nucleosides의 分析

조영현(Yung Hyun Cho),홍순근(Soon Keun Hong),박래정(Nae Jung Park) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.1

人蔘과 五加皮中의 核酸系 成分을 HPLC로 分離 定量하여 다음과 같은 결과를 얻었다.<br/> 1. 동일한 원료수삼에서 제조한 白蔘과 紅蔘의 核酸遊離鹽基와 nucleoside의 조성이 거의 같았다.<br/> 2. 人蔘과 五加皮중의 nucleosides 함량에서 adenosine, guanosine, cytidine 등이 特徵的인 差異를 나타냈으며 人蔘屬이 約 3倍이상 많게 나타났다. 그러나 tymidine과 inosine은 五加皮屬이 다소 많았다.<br/> 3. nucleobase 함량면에서는 人蔘이나 五加皮間에 비슷한 함량으로 1~2㎎/100g를 함유했다. Nucleobases and nucleosides in Panax and Acanthopanax genus were determined by high-performance liguid chromatography. Chromatomatography was performed on a reversed-phase system with μ Bondapak C_(18) column using phosphate buffer and 80% methanol gradient. Content of each nucleobase in two genera was about 0-2㎎/100g. Panax was contained guanosine and/or adenosine ca. 15-22㎎/100 g;and Acanthopanax guanosine ca. 3-8㎎/100g and adenosine ca. 2-7㎎/100g. Considerable amounts of cytidine, uridine, inosine, and thymidine were also detected in two genera.