투석 및 관류에 의한 살충제 포스파미돈의 제거 효율

홍세용,길효욱,양종오,이은영,Hong Sae Yong,Gil Hyo Wook,Yang Jong Oh,Lee Eun Young 대한임상독성학회 2005 대한임상독성학회지 Vol.3 No.1

Purfose: This study was to observe the phosphamidon reduction rate after haemoperfusion (HP) and Hemodialysis (HD) in vitro. Methods: We started off by measuring the clearance of HD and HP for the phosphamidon in vitro. Phosphamidon was measured hourly by High-pressure liquid chromatography. Results: Phosphamidon clearance was effectiveness in HP and HD. Phosphamidon reduction rate was no difference between HD and HP; $64\%$ versus $91.\%1$ at starting, $82.2\%$ versus $80.2\%$ at 1 hours, $82.2\%$ versus $73.8\%$ at 2 hours, $34.4\%$ versus $14.0\%$ at 3 hours, $14.1\%$ versus $27.4\%$ at 4 hours, $0\%$ versus $3.3\%$ at 5 hours. Conculsion: Extracorporeal elimination of phosphamidon is effective by hemoperfusio and hemodialysis in vitro. We suggest hemoperfusion may be effective in organophsphate intoxication patients.

한국인 만성 음주자 40 예에 대한 간조직학적 고찰

홍세용 ( Sae Yong Hong ),양승하 ( Seung Ha Yang ),김의한 ( Eui Han Kim ) 대한내과학회 1985 대한내과학회지 Vol.29 No.2

혈중 Paraquat 농도가 Paraquat 중독환자의 생존율에 미치는 영향

홍세용(Sae Yong Hong),양동호(Dong Ho Yang),(N N Sabapathy) 대한내과학회 1995 대한내과학회지 Vol.48 No.4

N/A Objectives: Assessment of the severity and prognosis of paraquat poisoning and the need to institute active therapy has been based on the quality alleged to have been taken and the intensity of the blue colour obtained on testing the urine with alkali/dithionite. Such arbitrary methods are fat from satisfactory and may be led to unnecessary vigorous treatment of some petients and, possibly, failure to treat others who might. have benefited. Material: 19 patients for whom the time of paraquat ingestion was known with reasonable certainty were admitted to Soonchyang University Chunan hospital, were investigated, Urine test with alkal/dithionite was positive in all of patients.Venous blood samples were collected on admission to the emergency room and repeatedly every 6hours during 12-18 hours. All patients has treated with same protocol in the text. Methods: Plasma paraquat concentrations were measured by radioimmunoassay. Urine paraquat concentratioons were measured by alkali/dithionite method. Prognostic significanse of plasma paraquat concentrations and the relation to urine concentration were described. Results: Plasma paraquat concentrations was not detected in 5 out of 19 patients and all of these patients survived. Plasma paraquat concentratopn was detected in 14 patients. It was distributed very widly. Among these 14 cases, 3 cases wer survivors and 11 cases died. In died patients, the peak levels of plasma parasquat ranged between 0.16mg/ml-3.13mg/ml (mediam;0.87mg/ml). Plasma paraquat concentration of the 3 survivors decreased to near zero levels in 12 hours. There was a significance relation between plasna parquat conentrations measurd by radioimmunoassay and ILD urine paraquat concentrations measured by alkali/dithionite method. Conclusion: Eliminatjon rate of plasma paraquat seems to be a more significant factor for the survival rate than the intiial plasma concentration of the paraqrat. Because of its simplicity, and low cost, urine paraquat conecentrations measured by alkali/ dithiontit method is coplementary to the plama paraquat conentrations measured by radioimmunoassay.

신경차단이 정맥압박에 의한 t - PA 분비에 미치는 영향

홍세용(Sae Yong Hong),양동호(Dong Ho Yang),신현길(Hyun Kil Shin),김순길(Sun Kil Kim),김난숙(Nam Suk Kim),김형미(Hyung Mi Kim),신정순(Jung Sunn Shin) 대한내과학회 1992 대한내과학회지 Vol.43 No.2

N/A Background: Fibrinoiytic response to venous occlusion of the limbs had been regarded as a sensitive and reliable method for assessing the individual fibrinolytic capacity, but the exact mechanism of the increased fibrinolytic activity after venous occlusion is not clear. Methods: In order to investigate the influence of neurotransmitter on t-PA release by venous occlusion, we compared the increase in t-PA and fibrinolytic activity in the euglobulin fraction initiated by cubital venous occlusion (100mmHg for 10mins), in pre-neural block and post-neural block states, in seven cases. In all patients, the supra-clavicular approach was used to obtain a brachial plexus block and venous occlusion was achieved at the cubital levels on the ipsilateral arm. Results: The euglobulin fibrinolytic activity before venous occlusion was 100.9±27,5 BAU in the pre nerve block state and 102.7±29.4 BAU in the post nerve block state (p>0.5). The t-FA antigen level before venous occlusion was 3.5±1.2ng/ml in the pre nerve block state and 4.0±1.0ng/ml in the post nerve block state (p>0.5). The increase in the euglobulin fibrinolytic activity after venous occlusion was 18.0±16.7 BAU in the pre nerve block state, and 18.3±15.6 BAU in the post nerve block state (p>0.5). The increase in the t-PA antigen levels after venous occlusion was 3.0±2.0ng/ ml in the pre nerve block state and 3.0±2.1ng/ml in the post nerve block state (p>0.5). Conelwion: These findings suggest that the peripheral nervous system does not exert any influence on t-PA release during venous occlusion.

Low Molecular Weight Heparin 이 Fibrinolytic Activity 에 미치는 영향

홍세용(Sae Yong Hong),양동호(Dong Ho Yang) 대한내과학회 1991 대한내과학회지 Vol.40 No.4

N/A To evaluate the influence of standard and low molecular weight heparin (LMWH) on intrinsic and extrinsic fibrinolytic activity, we measured total fibrinolytic activity in dextran sulfate euglobulin fraction, t-PA activity, and t-PA ag in plasma from 10 patients with cerebral infarction before and after heparin injection. The results were compared with that of a control day (on heparin injection day). The increase of total fibrinolytic activity in the 1.MWH group was significantly higher than in the standard heparin group (0. 025 < p < 0.05) and control group ((0.025<p<0,05). The t-PA activity increased in the at 1:00 P.M. samples in 3 cases of the standard heparin group 4 cases of the LMWH group and 4 cases of the saline group. But it decreased in 6 cases of the standard heparin group 6 cases of the LMWH group and 6 cases of the saline group. The change of t-PA activity and the rate of increase and decrease did not differ significantly between the groups (p>0.1 ANOVA). The t-PA ag of the 1:00 P.M. samples increased in 9 cases of the standard heparin group, 4 cases of the LMWH group and 7 cases of the saline group. But it decreased in 1 case of the standard heparin group, 6 cases of the LMWH group and 3 cases of the saline group, The change of t-PA ag and the rate of increase and decrease did not differ significantly between the group (p>0.1 ANOVA). The finding that LMWH in- creased the total fibrinolytic activity without an in- crease of extrinsic fibrinolytic activity suggests that LMWH increases intrinsic fibrinolytic activity.

제초제 Paraquat 중독환자에서 vitamin C 투여가 plasma total antioxidant capacity에 미치는 영향

홍세용(Sae Yong Hong),은수훈(Soo whon Eun),한찬희(Chan Hee Han),윤영근(Yung Kun Yoon),양동호(Dong Ho Yang),조성란(Suk Ran Cho),김휘준(Whi Jun Kim),한찬수(Chan Soo Han),박영현(Yung Hyun Park) 대한내과학회 2000 대한내과학회지 Vol.58 No.6

N/A Background : This study was undertaken to evaluate the effect of plasma concentration of vitamin C is on the plasma total antioxidant status(TAS) in patients with paraquat intoxication. Methods : As a preliminary research for this study, we measured plasma vitamin C levels, uric acid, TAS, and bilirubin from 100 healthy Koreans aged between 25-55 years. Various concentrations of vitamin C were constructed in vitro with normal pooled plasma between 1mg/dl and 100 mg/dL and TAS was measured. Both vitamin C concentrations and TAS were measured from the blood samples, taken at 0.5, 1, 2, 3, 5, 7, 9 hours after a bolus injection of vitamin C(50 mg/kg) from 7 volunteers who had been recruited from medical students at our hospital. Various amounts of vitamin C were given to 10 patients with paraquat intoxication for 5 consecutive days in the morning : first day ; 100 mg single dose, second day; 500 mg single dose, third day ; 1,000 mg single dose, fourth day : 3,000 mg single dose, and fifth day ; 3,000 mg three times every 8 hours. Results : The mean value of TAS and vitamin C measured in 100 healthy Korean adults was 2.22 ±0.16 mmol/L and 0.48±0.10 mg/dL. Age and sex do not influence these levels(p>0.05, data are not presented). A direct correlation was observed between TAS and vitamin C concentrations of 1 mg/dL and 100 mg/dL both in vivo and vitro. As the vitamin C concentration was increased gradually 5 consecutive days, the TAS values increased in the same way as follows : first day(vitamin C 100 mg) 2.26±0.98 mmol/L, second day(vitamin C 500 mg) 2.76±0.78 mmol/L, third day(vitamin C 1.000 mg) 2.81±0.68 mmol/L, fourth day(vitamin C 3.000 mg) 3.18 mmol/L. On the fifth day when 3.000 mg of vitamin C was given by bolus intravenous injection 3 times(every 8 hours for a day), the TAS values measured one hour after the injection was 3.58±0.37 mmol/L. Conclusion : When vitamin C is used as a free radical scavenger, the loading dose and maintenance dose should be around 2278 mg intravenous bolus and 146 mg/hour. High doses of vitamin C are effective and safe for patients with acute paraquat intoxication.(Korean J Med 58:666-674, 2000)

한국인 만성 음주환자에서 B 형 간염바이러스 표식자의 출현양상

홍세용 ( Sae Yong Hong ) 대한내과학회 1985 대한내과학회지 Vol.29 No.3

증례 : 급성 간헐성 포르피리아증에서 Hematin 치험 1 예

송병상 ( Byoung Sang Song ),홍세용 ( Sae Yong Hong ),김선주 ( Sun Joo Kim ) 대한내과학회 1985 대한내과학회지 Vol.28 No.6

원인 불명의 급성 신부전으로 발현된 급성 파라콰트중독 1례

길효욱,양종오,이은영,홍세용,Gil Hyo Wook,Yang Jong Oh,Lee Eun Young,Hong Sae Yong 대한임상독성학회 2004 대한임상독성학회지 Vol.2 No.1

Paraquat, a widely used herbicide, is extremely toxic, causing multiple organ failure in human. Many treatment modality has been used, but now paraquat is very fatal drug. Elimination rate of plasma paraquat seems to be a factor for the survival rate. So early diagnosis and early treatment are very important. Plasma paraquat concentration could be measured by radioimmunoassay. But it is impossible that the test was done at Emergency room and the result was checked immediately. There was relation between plasma paraquat concentrations and urine paraquat concentration. Because of its simplicity and low cost, urine paraquat concentration test is complementary to the plasma concentration measurement. If the patient has psychotic problem or unconscious mental state, and is observed unexplained dyspnea and oral ulcer, urine paraquat test is very important to rule out acute paraquat intoxication. We experienced a patient who was presented as unexplained acute renal failure initially and was diagnosed as paraquat intoxication later.

사구체 신염에서 lymphotoxin - α 의 유전자 다형성

이은영(Eun Young Lee),홍세용(Sae Yong Hong),최종순(Jong Soon Choi),양동호(Dong Ho Yang),조종태(Jong Tae Cho),윤성철(Sung Chul Yoon) 대한내과학회 2001 대한내과학회지 Vol.61 No.1

N/A Background: Lymphotoxin (LT)-α is a cytokine that has been involved in the inflammatory response, the pathogenesis of autoimmune disease, and the development and progression of renal injury in various glomerulonephritis. Glomerulonephritis is an important cause of end-stage renal disease requiring renal replacement therapy. A great body of evidence suggests that immunologic mechanisms and genetic factor such as gene polymorphism play an important role in the renal injury of glomarulonephritis. However, the role of LT-α gene polymorphism in primary glomerulonephritis is not clear yet. The purpose of this study is to examine whether there are the associations between LT-α gene polymorphisms and the development and progression of various primary glomerulonephritis. Methods : A cross-sectional study of LT-α gene polymorphism by polymerase chain reaction with restriction fragment length polymorphism was performed on 190 patients with primary glomerulonephritis confirmed by renal biopsy and 249 controls. The gene polymorphism in the first intron of LT-α gene was detected by digestion with Ncol restriction enzyme and subsequent electrophoresis. The clinical parameters at renal biopsy and the latest follow-up were collected. Results : LTA1/LTA1: LTA1/LTA2: LTA2/LTA2 genotype distribution of the LT-α gene in the study population was 0.12: 0.54: 0.34, and the LTA1: I.TA2 allele frequency was 0.39: 0.61. Thirty-one minimal change disease, 33 focal segmental glomerulosclerosis, 18 membranous glomerulonephritis, 16 membranoproliferative glomerulonephritis, and 92 InA nephropathy were included in this study. The distribution of LTA1/LTA1: LTA1/LTA2: LTTA2 was significantly different in the membranous glomerulonephritis (0.33: 0.39: 0.28, ρ<0.001) with control groups. But, there were no significant differences between the distributions of LT-α genotype in the patients with other glomerulonephritis groups. There were no significant differences in the age, systolic and diastolic blood pressure, serum creatinine, cholesterol, and proteinuria at renal biopsy among the three genotypes. There was no significant difference in the incidence of patients who started dialysis treatment or whose serum creatinine was double or more during the follow-up duration which was more than four years among the three genotypes. Conclusion : These data suggested that polymorphism in the LT-α gene may be associated with the development of primary membranous glomerulonephritis, however, these are not directly associated with the progression of renal injury in various glomerulonephritis.(Korean J Med 61:10-16, 2001)