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췌조직과 성장 발육에 따른 흰쥐 조직내 S-Adenosylmethionine Synthetase 활성도 및 S-Adenosyl-L-methionine의 분포
박승희,유태무,홍성렬,이향우,Park, Seung-Hee,Yu, Tae-Moo,Hong, Sung-Youl,Lee, Hyang-Woo 대한약학회 1994 약학회지 Vol.38 No.4
S-Adenosyl-L-methionine synthetase (ATP: methionine S-Adenosyltransferase, EC 2.5.1.6; AdoMet synthetase) catalyzes the biosynthesis of S-Adenosyl-L-methionine(AdoMet) from methionine in the presence of ATP. To elucidate the role of transmethylation reaction in the pancreatic tissues, we examined AdoMet synthetase and isozyme activities, and AdoMet contents in the various tissues. The activities of AdoMet synthetase marked the highest in the kidney, and the lowest in the testis among the various tissues of rat. Considerable amounts of AdoMet synthetase activities were detected in the pancreatic tissues of various animals except for those of frog. The level of ${\alpha}$ and ${\gamma}$ isozyme activities were present in the pancreatic tissues of various animals, while ${\beta}$ isozyme activities were detected as trace. AdoMet synthetase activities of rat brain, liver, testis were decreased with growth. In the rat pancreatic tissues, AdoMet synthetase activities were increased during 16 days after birth and then decreased between 16 and 47 days of age. Levels of AdoMet contents of rat brain and testis were decreased with growth. However, AdoMet contents of rat pancreas were decreased until 26 days of age, and then increased thereafter. AdoMet synthetase isozyme patterns did not vary with growth in the pancreas and testis. But, in the liver, ${\beta}$ form is strikingly increased with growth.
누룩(Rhizopus oryzae KSD-815)으로부터 분리한 지질화합물의 세포독성 및 항염증 활성
곽호영 ( Ho Young Kwak ),이상진 ( Sang Jin Lee ),이대영 ( Dae Young Lee ),배낙현 ( Nark Hyun Bae ),정낙훈 ( La Koon Jung ),홍성렬 ( Sung Youl Hong ),김계원 ( Gye Won Kim ),백남인 ( Nam In Baek ) 한국응용생명화학회 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.2
흰쥐에서 신델라 겔 (송아지 제단백혈액추출물 : 황산미크로노마이신=20:1 복합제제)의 30일간 반복투여 경피독성시험
남석우(Suk Woo Nam),성대석(Dae Suk Sung),유세근(Se Keun Yoo),장만식(Man Sik Chang),최완수(Wahn Soo Choi),정영국(Young Kuk Chung),김규봉(Kyu Bong Kim),한정환(Jeung Whan Han),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 대한약학회 1997 약학회지 Vol.41 No.2
This study was conducted to investigate the subacute transdermal toxicity of Syndella gel, a new topical drug containing deproteinized dialysate of calf''s blood and micronomicin sulfate in Sprague-Dawley rats. Three doses (1.97, 3.94, 7.88 g/kg) of Syndella gel was daily treated transdermally to male and female rats for 30 days. No death was occurred in either control or treated rats. No significant toxic clinical signs and body weight change were not observed at any doses in the male or female rats treated. There were no significant alterations in hematologic and biochemical parameters in both sexes, however slight increase of potassium concentration was observed in 3.94g/kg and 7.88 g/kg female groups. No significant necrotic changes were not observed in examined organs. This study showed that up to 7.88g/kg Syndella gel did not induce subacute transdermal toxicity.
Curcumin이 microglia의 활성화에 미치는 영향
정기경(Ki Kyung Jung),이상진(Sang Jin Lee),이선우(Sun Woo Yi),강석연(Seog Young Kang),김태균(Tae Gyun Kim),강주혜(Ju Hye Kang),홍성렬(Sung Youl Hong),주일로(Ilo Jou),김승희(Seung Hee Kim),한형미(Hyung Mee Han) 대한약학회 2000 약학회지 Vol.44 No.5
Microglia, brain resident macrophages, play a central role in the inflammatory responses of the brain and are activated in brain injuries and several neurodegenerative diseases such as Alzheimer's and Parkinson's disease, thereby aggravating the course of these diseases. In this study, the effects of plant-derived compounds such as curcumin or gingerol on the microglial activation were examined. Microglial cultures were prepared from 2-3 week mixed primary glial cultures obtained from the cerebral cortex of 1-2 day old rats and identified by immunocytochemistry using microglial-specific antibody OX-42. Microglia were activated by lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) and the effect of curcumin or 6-gingerol on the microglial activation was examined. Specific parameters measured to monitor microglial activation were nitric oxide (NO), prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-alpha) release. Curcumin (1-10mcM) inhibited NO release induced by LPS and IFN-gamma in a dose-dependent manner whereas 6-gingerol (2-20mcM) did not have any effect on LPS/IFN-gamma-induced NO release. The levels of PGE2 and TNF-alpha induced by LPS and IFN-gamma were also inhibited by 1-10pM curcumin in a dose-dependent manner. These results showed that curcumin could modulate microglial activation.
남석우(Suk Woo Nam),박승희(Seung Hee Park),윤성필(Sung Pil Yoon),서동완(Dong Wan Seo),남태균(Tae Kyun Nam),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.2
Effects of Biozyme^R and Business^R on alcohol metabolism in rats, and on the activities of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH) were studied in vitro. Alcohol concentration in rat blood was decreased after the treatment of Business(3.3 ㎖/㎏, Biozyme 1.67 ㎎/㎖) and Biozyme(3.3 ㎖/㎏, 1.67 ㎏/㎖) prior to the administration of ethanol(25%, 0.83 g/㎏). And the acetaldehyde concentration of rat blood was also decreased when compared with control values in the same condition. Effects of Biozyme on ADH and ALDH activity were also studied. While the ALDH activity was elevated in the presence of Biozyme(2 ㎍/assay), the ADH activity was not influenced by Biozyme at the concentration range from 2 ㎍/assay to 0.2 ㎎/assay. In summary, Biozyme accelerated the rate of ethanol metabolism and the acceleration might be due to the increase in ALDH activity.
단백질 메틸화효소류 및 S-아데노실-L-메치오닌 연결효소의 활성도에 미치는 사염화탄소-유발 간독서의 영향
남궁석민(Suck Min Namkoong),유태무(Tae Moo Yoo),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 대한약학회 1992 약학회지 Vol.36 No.1
In order to test relationships between hepatotoxicity and transmethylation, activities of protein methylases and SAM (S-adenosyl-L-methionine)-synthetase wee examined in liver tissues of rats treated with CCl4. Also the concentrations of SAM and SAH were measured by HPLC in rat liver. The results are as follows. (1). Activities of protein methylases were not significantly changed in 24 hours after CCl4 treatment. However, in 48 hours, activities of protein methylases were significantly increased in comparison with that of control. (2). Activity of SAM-synthetase was increased steadily in the time course after CCl4 treatment.(3). S-adenosyl-L-methionine concentration of liver tissues in CCl4-treated group was elevated in 24 hours, and then decline thereafter. But the SAH concentration was slightly decreased in the time course after CCl4 treatment. These results indicated that SAM was very actively used in transmethylation reactions of CCl4 damaged rat liver, suggesting the strong relationships between hepatotoxicity and transmethylation phenomena.
닭 췌장 Protein Methylase II의 분리정제 및 성질
유태무(Tae Moo Yoo),남궁석민(Suck Min Namkoong),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 대한약학회 1991 약학회지 Vol.35 No.6
Protein methylase II (S-adenosyl-L-methionine:protein carboxyl-0-methyltransferase; EC 2.1.1.24., PM II) was purified from chicken pancreas by subcellular fractionation, DEAE-cellulose chromatography, QAE-Sephadex A-50 chromatography, Sephadex G-75 chromatography, and Sephadex G-75 rechromatography. The purified PM II gave a single band upon polyarcrylamide gel electrophoresis both in the presence of SDS and in Tris glycine buffer without SDS. The pI value of purified PM II was identified as 5.7 on isoelectric focusing gel. Properties and activities of PM II were studied and the following results were obtained. 1) PM II from chicken pancreas was purified approximately 221-fold with a yield of 1.3%. 2) The purified PM II appear constituted of a single polypeptide chain of a molecular weight 46,800 daltons. 3) Hemoglobin exhibited the highest of methyl-accepting activity among the substrates tested. 4) The purified PM II has a Km of 4.67 X 10-6M and a Vmax of 37.5 pmoles of methyl-14C/min/mg enzyme for SAM-14CH3 as methyl donor in the presence of histone type II-As. 5) It is found that S-adenosyl-L-homocysteine is a competitive inhibitor for PM II with KI value of 3.23 X 10-5M.
랫드에서 인체 재조합 적혈구 조혈인자, rHuEPO의 13주 정맥투여 아만성독성에 관한 연구
김형식,곽승준,천선아,박현선,한하수,임소영,안미영,김원배,안병옥,홍성렬,이병무,Kim, Hyung-Sik,Kwack, Seung-Jun,Chun, Sun-Ah,Park, Hyun-Sun,Han, 한하수,Lim, So-Young,Ahn, Mi-Young,Kim, Won-Bae,Ahn, Byoung-Ok,Hong, Sung-Youl,Lee, Byung-Mu 한국독성학회 1998 Toxicological Research Vol.14 No.3
A recombinant human erythropoietin (rHuEPO) was administered intravenously at dosage levels of 0, 100, 500, and 2500IU/kg/day for a period of 3 weeks. There were no observed clinical signs and deaths related to treatment in all groups tested. Decreases in body weight gain and food consumption were observed only in males of 2,5000IU/kg group after 2 weeks. In hematological parameters, erythrocyte content, hematocrit values and hemoglobin concentration were dose- dependently increased in rHuEPO treated groups. The ratio between kidney weight and whole body weight was significantly increased in females of 500 and 2,500IU/kg groups. The spleen weight was also increased in both sexes of 500 and 2,500IU/kg groups. However, the absolute weight change of other organs was not observed. In histopathological examinations, the renal tubular basophilia was observed only in males and females of 2,500IU/kg groups. From these results, it is concluded that the no-observed adverse effect level (NOAEL) of rHuEPO is 100 IU/kg in rats in the present study.
박창원(Chang Won Park),조대현(Dae Hyun Cho),홍성렬(Sung Youl Hong),한정환(Jeung Whan Han),이향우(Hyang Woo Lee) 대한약학회 1998 약학회지 Vol.42 No.6
The liver expresses a considerable amount of nitric oxide (NO) upon induction with cytokines or/and endotoxin. The NO synthesized by inducible NO synthase (NOS) of the liver seems to play a role in various hepatic physiological processes. Here we investigate the effects of NO on acetaminophen (AA)-induced liver injury. The treatment of S-nitros-N-acetyl penicillamine (SNAP, exogenous NO donor) at the dose of 0.1mM decreased AA-induced hepatotoxicity suggesting the possibility of NO to play a role in protection from the hepatotoxicity induced by AA. On the other hand, the excessive NO produced by NO donor (SNAP: 0.5, 2.5, 6.25mM) has been shown to cause a concentration dependent hepatotoxicity, and such damages was decreased by Superoxide and increased by superoxide dismutase, indicating that the hepatotoxicity induced by excessive NO depends on balancing between NO and superoxide. Taken together, the results indicate that NO has biphasic effects on hepatotoxicity.