우리나라 식물원·수목원의 이용자 만족도 분석

현정오(Jung-Oh Hyun),김용식(Yong-Shik Kim),신현탁(Hyun-Tak Shin),강신구(Shin Koo Kang),김종익(Jong-Ik Kim),김종근(Jong-Geun Kim),윤정원(Jung-Won Yoon),이명훈(Myung-Hoon Yi) 한국산림휴양학회 2012 한국산림휴양학회지 Vol.16 No.1

우리나라 수목원의 나아가야할 방향을 제시하고자 공립수목원 3개소, 사립수목원 3개소 탐방객을 대상으로 설문을 통해 이용자 만족도를 분석하였다. 탐방객들이 인식하는 수목원의 기능 변수에서는 보전 기능만 유의하였다. 방문동기를 살펴보면 공립수목원의 경우 접근성 및 저비용으로 인해 방문 기회가 용이한 반면, 수목원을 공원으로 인식하는 경향이 우려된다. 또한 탐방객은 수목원 이용 후 자연의 아름다움, 자연에서 느낀 혼자만의 시간, 접근성 및 저비용 측면에서 만족감을 나타내었다. 마지막으로 시설물 이용 만족도 평가 결과 주차장, 화장실, 식물, 경관의 4항목에 서 주차장의 위치, 수용능력, 관리상태, 화장실의 위치, 청결상태, 미관, 관리상태, 식물다양성, 경관의 식물 특이성 변수에서 유의한 것으로 나타났다. 식물 다양성 및 식물 특이성 변수로 볼 때 공립수목원 보다는 사립수목원에서 보다 다양한 식물의 수집 및 교환이 이루어지고 있어 보다 다양한 식물을 접하는 것으로 나타났다. 전반적으로 공립수목원은 종 수집, 전시, 보전적 측면에의 노력을 통해 수목원 본연의 기능을 강화해야할 것으로 판단된다. In order to present domestic arboreta's path to go, a questionnaire was conducted regarding user's satisfaction on visitors to three public and three private arboreta. Among functional variables recognized by the visitors, only conservation function was significant. As for their motives for visiting arboreta, accessibility and low costs enabled ready visits to public arboreta. However, they tended to recognize arboreta as parks, which is worrisome. They also expressed satisfaction with the beauty of nature, time spent alone within nature, accessibility, and low costs. The result of evaluation of their satisfaction with facilities?parking lots, rest rooms, plants, and landscape?showed that variables such as the locations of parking lots, their capacity, cleanliness, fine views, management conditions, plant diversity, and plant specificity in landscape were significant. In terms of plant diversity and specificity, private arboreta collected and exchanged more diverse plants than public arboreta and therefore visitors could experience more various plants in private arboreta. Overall, it is judged that public arboreta should strengthen their original functions by making efforts in collection of species, exhibition, and conservation.

북미사시나무 trembling aspen ( Populus tremuloides Michx . ) 근단조직내의 (根端組織內) 동위효소들의 유전

현정오 ( Jung Oh Hyun ) 한국산림과학회 1984 한국산림과학회지 Vol.64 No.1

The inheritance of isoenzymes in young coat tissues of trembling aspen (Populus tremuloides Michx.) was studied by electrophoretically analysing five parental clones and their full-sib progenies obtained by crossing one female clone to four male clones. The distal 2㎝ section of vigorous young roots of 70 seedlings per family were subjected to horizontal starch gel electrophoresis. The resulting gels were tested for activity of 7 enzyme systems. Evidences for the inheritance of isoenzymes observed indicated that the isoenzyme variants of every isoenzyme zone were under control of codominant alleles at a single locus. Chi-square test of joint segregation data of the two loci, 6-PGD-2 and PGI-2, indicated that the pair of loci was not linked.

아연폐광산(亞鉛廢鑛山) 주변(周邊) 토양(土壤)의 중금속(重金屬) (Cd, Cu, Zn, Pb) 오염(汚染)에 따른 5개(個) 수종(樹種)의 부위별(部位別) 중금속(重金屬) 축적(蓄積)

한심희,현정오,이경준,조덕현,Han, Sim Hee,Hyun, Jung Oh,Lee, Kyung Joon,Cho, Duck Hyun 한국산림과학회 1998 한국산림과학회지 Vol.87 No.3

본 연구는 폐광지 주변에서의 토양내 중금속(Cd, Cu, Zn, Pb) 오염 정도와 토양내 중금속 농도와 수목내 중금속 축적 농도와의 관계를 규명하여, 오염지역의 정화에 수목을 이용할 수 있는지를 확인하는 것을 목적으로 수행하였다. 경기도 화성군에 위치한 삼보광산과 광명시에 있는 가학광산에서 1997년 4월부터 9월에 걸쳐 토양 시료와 주변의 난티잎개암나무, 리기다소나무, 현사시나무, 진달래, 아까시나무를 채취하여 중금속 농도를 측정하였다. 두 광산지역의 광구에서 500m 이내 주변 토양은 중금속에 의한 오염 정도가 28~143ppm으로써 심각하지 않았으나, Zn(143ppm), Pb(97ppm)과 같은 중금속 농도는 독성을 나타낼 수 있는 수준이었다. 토양내 중금속의 농도는 Zn>Pb>Cu>Cd의 순이었으며, 광구의 중심에서 1.5km 이상 멀어질수록 증금속 농도는 10ppm 이내로 감소하였다. 각 수종별 중금속의 농도는 Pb를 제외하고, 현사시나무에서 가장 높았으며, 특히 잎에 고농도로 축적되어 있었고, Zn의 경우 91ppm이 검출되었다. 난티잎개암나무의 뿌리에서는 Cu와 Pb의 높은 농도로 검출되었다. 토양의 중금속과 수목 조직내 중금속 농도는 매우 높은 정의 상관을 보여주었으며, 리기다소나무에서 가장 높은 상관계수를 나타냈다. 토양에 대한 수목 조직의 중금속 농도비(Concentration Factors : CF)는 Zn이 가장 높은 값을 보였고 Pb이 가장 낮은 값을 보여주었다. 현사시나무는 CF값이 가장 높아서 중금속을 가장 많이 축적하고 있었다. 특히 토양의 중금속 농도에 대한 식물체의 농도비가 가장 높은 현사시나무는 중금속의 흡수 능력이 뛰어나 중금속 오염지에서 정화식물로 이용할 수 있으며, 리기다소나무는 토양의 오염 정도를 가장 잘 반영하는 수종으로 판단된다. This study was conducted to evaluate heavy metal concentrations(Cd, Cu, Z, and Pb) in the soil of two zinc mines and to correlate heavy metal contents between the soils and trees growing near the mines. Soils and leaves, stems, and roots of five tree species(Corylus heterophylla, Pinus rigida. Populus alba${\times}$glandulosa, Rhododendron mucronulatum, and Robinia pseudoacacia) were collected from Sambo Zinc Mine located in Hwasung and Gahak Zinc Mine in Kwangmyung city in Kyonggido. Soils near two zinc mines were not seriously contaminated by heavy metals, but Zn and Pb concentrations were at toxic level. The heavy metal concentration in soils decreased in the order of Zn, Pb, Cu, and Cd, and decreased with increasing distance from zinc mining sites. Among the five tree species, Populus alba ${\times}$ glandulosa showed the highest heavy metal concentrations in the tissue except for Pb. Particulars, leaves of the species contained the high concentrations of heavy metals to reach the maximum of 91ppm Zn. The roots of Corylus heterophylla contained high concentrations of Cu and Pb. The order of heavy metal concentrations in the tree species was Zn, Cu, Pb and Cd. The concentration of heavy metals in the tree tissues showed a positive correlation with that in soil in which trees are growing. The ratio of heavy metal concentration of trees to that of soils(concentration factor : CF) was highest in Zn and lowest in Pb. Populus alba${\times}$glandulosa had the highest CF value among the five tree species. It was concluded that Populus alba${\times}$glandulosa, based on the high metal uptake ability, could be used for decontaminating of heavy metals from contaminated soils, and Pinus rigida could be used to reflect the level of contamination in soils.

An investigation on applicability of isozyme band patterns for identification of poplar clones

Jung Oh Hyun(玄正悟),Sin Kyu Hyun(玄信圭) 한국육종학회 1993 한국육종학회지 Vol.25 No.1

This paper describes the applicability of the isozyme band patterns produced by horizontal starch gel electrophoresis for identification of clones belong to genus Populus. For isozyme analysis, one to two-weeks-old leaf tissues of the clones were subjected to horizontal starch gel electrophoresis and the resulting gels were stained with four enzyme systems. The band patterns of the variable enzyme systems were characterized by using densitometer and all the distinct band patterns(gel phenotypes) were numbered. One hundred and fifteen clones out of 119 clones could be identified based on their particular band patterns of the four enzyme systems. However, the isozyme band patterns of MDH was changed every week after two weeks from leaf flushing as leaves expand and mature indicating that reproducibility of clone specific gel phenotypes for MDH is limited in a very short period of a developmental stage. Thus, using isozyme band patterns for identification and registration of poplar clones requires careful selection of either enzyme systems that would produce stable isozyme band patterns throughout developmental stages and environmental conditions or sample collection time in a specific developmental stage.

산칠량이 (産漆量) 많은 옻나무개체의 선발에 관한 연구

현정오,김만조,이세표 ( Jung Oh Hyun,Mahn Jo Kim,Shae Pyo Lee ) 한국산림과학회 1993 한국산림과학회지 Vol.82 No.2

This study was conducted to investigate characteristics related to lacquer yield and to evaluate possibility of indirect selection of Rhus verniciflua Stokes. Twenty-four lacquer trees were randomly selected for measuring growth, urushiol content and bark thickness. Urushiol content showed a significant positive correlation with outer bark thickness and secretory canal density at 1% level, and with inner bark thickness at 5% level. Bark thickness and secretory canal density could be used as indicators for high urushiol content within bark and thus for high lacquer yield. Mass selection for high lacquer yield was made based on urushiol content within bark. Sixteen individuals with the highest urushiol content value were selected for breeding. As expected, the selected trees had thicker bark. higher density of secretory canal, better developed secretory canals compare to the non-selected lacquer trees.

참나무류의 원형질체 분리 및 융합에 (融合) 영향을 끼치는 요인에 관한 연구

현정오,김재헌,장석소 ( Jung Oh Hyun,Jae Hun Kim,Suk So Chang ) 한국산림과학회 1985 한국산림과학회지 Vol.71 No.1

Factors affecting isolation and fusion of protoplasts of three Quercus species were investigated and procedures for isolation, purification and fusion of protoplasts of the three species were also established. Unhardened leaves and rapidly growing callus cultures were good source of viable protoplasts. The optimum composition of enzyme mixture for rapid isolation of protoplasts from leaf mesophyll tissues and calli was Cellulase Onozuka R-10 (20g/ℓ, Macerozyme R-10(10g/ℓ), Pectinase(250 units/ℓ, CaCl₂·2H₂O(14mM), MgSO₄·7H₂O(1.8mM), KNO₃(1.0mM), H₃BO₃(1.0mM), KH₂PO₄(0.2mM), KI(1.0μM), 1,4-dithiothreitol (0.1mM) and mannitol (0.6M). Optimum density of protoplasts for maximum fusion was 2×10^5/㎖ which was the highest protoplast density given in this study. Optimum concentration and duration of PEG 1450 treatment for inducing fusion appeared to be 29%(W/V) final PEG 1450 concentration and 5-10 minutes, respectively.

아연폐광산 주변 토양의 중금속 ( Cd , Cu , Zn , Pb ) 오염에 따른 5개 수종의 부위별 중금속 축적

한심희,현정오,이경준,조덕현 ( Sim Hee Han,Jung Oh Hyun,Kyung Joon Lee,Duck Hyun Cho ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.3

This study was conducted to evaluate heavy metal concentrations(Cd, Cu, Z, and Pb) in the soil of two zinc mines and to correlate heavy metal contents between the soils and trees growing near the mines. Soils and leaves, stems, and roots of five tree species(Corylus heterophylla, Pinus rigida. Papulus alba × glandulosa, Rhododendron mucronulatum, and Rabinia pseudoacacia) were collected from Sambo Zinc Mine located in Hwasung and Gahak Zinc Mine in Kwangmyung city in Kyonggido. Soils near two zinc mines were not seriously contaminated by heavy metals, but Zn and Pb concentrations were at toxic level. The heavy metal concentration in soils decreased in the order of Zn, Pb, Cu, and Cd, and decreased with increasing distance from zinc mining sites. Among the five tree species, Populus alba × glandulasa showed the highest heavy metal concentrations in the tissue except for Pb. Particulars, leaves of the species contained the high concentrations of heavy metals to reach the maximum of 91ppm Zn. The roots of Corydus heterophylla contained high concentrations of Cu and Pb. The order of heavy metal concentrations in the tree species was Zn, Cu, Pb and Cd. The concentration of heavy metals in the tree tissues showed a positive correlation with that in soil in which trees are growing. The ratio of heavy metal concentration of trees to that of soils(concentration factor : CF) was highest in Zn and lowest in Pb. Populus alba×glandulosa had the highest CF value among the five tree species. It was concluded that Populus alba×glandulosa, based on the high metal uptake ability, could be used for decontaminating of heavy metals from contaminated soils, and Pinus rigida could be used to reflect the level of contamination in soils.

RAPD 표식자 분석에 의한 사시나무속 Leuce 절 포플러의 유연관계

홍강낙,현정오,홍용표 ( Kyung Nak Hong,Jung Oh Hyun,Yong Pyo Hong ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2

Genetic relationships of some poplars in the section Leuce, including 5 species and 11 clones of Populus alba×glandulosa, were investigated on the basis of RAPD marker analysis. Twenty-two of the 88 arbitrary 10-mer primers, showed reproducible amplification in the preliminary experiment with 6 samples, were used for PCR and generated a total of 181 RAPD markers. Genetic relationships among the analyzed samples were tested by two phenetic methods of the UPGMA and the neighbor-joining, which revealed the close genetic relationship between P. glandulosa and P. alba. And the close genetic relationship between P. glandulosa and P. davidiana was ascertained by the principal component analysis. Based on the observation of the close genetic relationship between them, it was deduced that P. glandulosa might be originated by the saltational speciation caused by the hybridization between P. alba and P. davidiana in nature.

Inverse PCR 기법을 이용한 양황철 DNA 의 Regulatory Region 의 탐색

손석규,현정오 ( Suk Gyu Son,Jung Oh Hyun ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.3

This research was conducted to identify plant regulatory regions by gene tagging method. A promoterless GUS coding sequence was introduced to Populus nigra × P. maximowiczii via Agrobacterium strains(LBA4404/EHA101), and putative transgenic poplars were selected by culturing on medium containing G418(60㎎/ℓ) and by GUS assay. Among them one positive plant was to amplify the native sequences flanking to the introduced GUS gene in plant genome by inverse PCR method and from this 730 by DNA product was obtained. After subcloning and sequencing, it has 88% homology to the Eucalyptus gunnii CAD(cinnamyl alcohol dehydrogenase) gene. The GUS gene fused with the putative promoter reinserted into poplar leaves by particle bombardment method to test the funtional promoter activity. Upon staining with X-gluc, many blue sprots appeared on the leaf segments bombarded by the chimeric gene 2-3 days, thus the isolated DNA fragment contain some possible coding region as well as a putative regulatory sequences of poplar CAD gene.

Agrobacterium tumefaciens vector 를 이용한 양황철의 형질전환 (

손석규,현정오 ( Suk Gyu Son,Jung Oh Hyun ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2

This study was conducted to find the optimum transformation condition using Agrobacterium harboring promoterless GUS gene. The optimal medium for shoot induction from leaves of Populus nigra×P. maximowiczii was MS medium supplemented with 0.1㎎/ℓ NAA, 0.5㎎/ℓ BAP(94% regeneration frequency and 11.5 average number of shoot) According to the test using pBI121, the concentration of antibiotics for selection marker gene was 100㎎/ℓ kanamycin or 60㎎/ℓ geneticin in the SIM(shoot inducing medium) 3. Two weeks later, callus was induced in the SIM 3 and this callus grew up to 0.5-1cm shoots after 6 weeks in the new SIM 3. And the treatment with methylation inhibitor(5-azacytidine) led to a dramatic increase in foreign gene expression rate from 5.7% to 26.7% . The vector systems showed. different transformation efficiencies based on the fluorometric and histochemical GUS assay. In this study the vector systems used for transformation seemed to affect transformation frequency, in which pEHA101 yielded more transformants(35.9%) than LBA4404/pBI121 did(5.7%). This result indicated that pEHA101 was effective to insert the promoterless foreign gene into a poplar genome.