Genetic Diversity and Population Structure of Liriope platyphylla (Liliaceae) in Korea

허홍욱,최주수,이복규,허만규,Huh, Hong-Wook,Choi, Joo-Soo,Lee, Bok-Kyu,Huh, Man-Kyu Korean Society of Life Science 2007 생명과학회지 Vol.17 No.3

한국내 분포하는 맥문동(Liriope platyphylla) 11집단에 대한 20 알로자임 대립유전자좌위에서 유전적 다양성과 집단구조를 조사하였다. 효소내 다형성을 나타내는 빈도는 55.9%였다. 종과 집단 수준에서 유전적 다양도는 각각 0.178, 0.168로 높았으며, 집단간 분화 정도는 낮았다($G_{ST}$ = 0.064). 전체 11 집단에서 임의교배에 의한 편차는 0.311이였다. 전체 유전적 다양성는 $0{\sim}0.535$였다. 유전적 다양도 중 집단내 변이는 높았다($H_S$ = 0.305). 세대간 이주하는 개체수는 약 3.66으로 이 종의 한국내 집단간 유전자 흐름이 높음을 시사한다. 또한 라이트의 고정지수 분석 결과 많은 대립유전자좌위와 집단에서 이형접합자의 결핍이 존재하고 있었다. 집단간 유전적 동질성은 0.988이였다. 이는 맥문동의 분포지가 한국내 유사한 환경에 놓여 있고 집단이 방향적 동질성을 가지고 있음을 시사한다. Genetic diversity and population structure of eleven Liriope platyphylla (Liliaceae) populations in Korea were determined using genetic variation at 20 allozyme loci. The percent of polymorphic loci within the enzymes was 55.9%. Genetic diversity at the species level and at the population level was high(Hes = 0.178; Hep = 0.168, respectively), whereas the extent of the population divergence was relatively low ($G_{ST}$ = 0.064). $F_{IS}$, a measure of the deviation from random mating within the 11 populations, was 0.311. Total genetic diversity values ($H_T$) varied between 0.0 and 0.535, giving an average over all polymorphic loci of 0.323. The interlocus variation in within population genetic diversity ($H_S$) was high (0.305). An indirect estimate of the number of migrants per generation (Nm = 3.66) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Mean genetic identity between populations was 0.988. It is highly probable that directional toward genetic uniformity in a relatively the homogenous habitat is thought to be operated among Korean populations of L. platyphylla.

ISSR을 이용한 고추나물 집단의 유전적 다양성과 계통학적 연구

허홍욱,허만규,강동호,Huh, Hong-Wook,Huh, Man-Kyu,Kang, Dong-Ho 한국생명과학회 2007 생명과학회지 Vol.17 No.6

Inter simple sequence repeat (ISSR) markers were performed in order to analyse the phylogenetic relationships of eight Hypericum electum populations in Korea. The six primers were produced 37 reproducible ISSR bands. Analysis of ISSR from individual plants of Korean H. erectum resulted in 22 polymorphic bands with 59.5%. Across populations, the mean number of alleles per locus was 1.348 and Shannon's information index was 0.203.Population Mt. Gyeryong had the highest expected genetic diversity (0.175) among all populations. When species were grouped by eight populations, within group diversity was 0.140 (Hs), while among group diversity was 0.472 (G$_{ST}$) on a per locus basis. The estimated gene flow (Nm) for H. erectum was very low (0.561). It is suggested that reproductive isolation by the isolation of geographical distance among H. electum populations and genetic drift may have played roles in shaping the population structure of this species. In phonetic tree, all populations were well separated from each other. Thus, ISSR markers are very effective in classifying natural population levels of genus Hypericum in Korea.

연구논문 : 인공 산성비가 중요 농작물의 생육에 미치는 영향

허홍욱(Hong Wook Huh),허만규(Man Kyu Huh) 한국환경과학회 1998 한국환경과학회지 Vol.7 No.2

Genetic Diversity and Relationships between Wild and Cultivated Radishes in Korea Revealed by RAPD Markers

Hong Wook Huh(허홍욱),Man Kyu Huh(허만규),Joo Soo Choi(최주수) 한국원예학회 2001 한국원예학회 학술발표요지 Vol.2001 No.5

한국산 갯무 자연집단의 유전적 다양성

허만규(Man Kyu Hur),허홍욱(Hong Wook Huh),정명기(Myong Gi Chung) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.4

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시루봉의 산불 이후 식생회복

허만규,최주수,정영기,허홍욱,문성기,Huh Man Kyu,Choi Joo Soo,Jeong Yong Kee,Huh Hong Wook,Moon Sung Gi 한국생명과학회 2005 생명과학회지 Vol.15 No.1

The recovery of vegetation in the burned area after forest fire was investigated in Mt. Sirubng, Jinju city, from 1994 to 2000. The floristic composition of a burned area was 37 kinds in 2000. The life from spectra of six sites showed H-D1-R5-e type. This type is similar to the life form which has been usually showed in most of burned areas. Total diversity index of six burned sites showed 2.135 (1996), 3.784 (1998), and 2.948 (2000). whereas, total diversity index of six unburned sites was 2.642 (1996), 2.516 (1998), and 2.723 (2000). Evenness index of six sites showed 1.816 (1996), 2.641 (1998), and 1.925 (2000). The dominant index of burned and unburned areas were 0.012 and 0.250 (1996), 0.031 and 0.261 (1998, and 0.110 and 0.275 (2000), respectively. The degree of succession in the unburned area gradually increased and the burned area was recovered to be similar to the unburned area after three years. 경상남도 진주시 수곡면 시루봉에서 산불이 난 1994년부터 2000년까지 식생회복에 따른 종조성을 조사하였다. 소나무를 비롯한 목본과 풍산포 종자를 가진 초본류는 산불 이후 3년 이내에 왕성한 성장을 보였다. 산화지의 생활형 조성은 H-Dl-R5-e로 다른 산화지와 크게 다를 바 없었다. 산화지 6지점의 평균 종다양도지수는 2.135(1996년), 2.784(1998년), 2.948(2000년)이였다. 6지점의 균등성지수는 1.816(1996년), 2.641(1998년), 1.925(2000년)였다. 천이 분석에서 식생의 회복과 더불어 종수는 증가하여 산화 3년 후에는 주변지역과 거의 유사하거나 약간 높은 지점도 나타났다. 우점도지수 등의 결과에서 추정되는 바는 산불 후 수목의 식재시 산불 후 3년 이전에 실시하는 것이 적합할 것으로 사료된다

RAPD를 이용한 한국산 층층나무속(Cornus)의 계통분류학적 유연관계 연구

김세영 ( Se Young Kim ),허홍욱 ( Hong Wook Huh ) 부산대학교 과학교육연구소 2008 교사교육연구 Vol.47 No.3

층층나무는 북동 아시아 지역에 주로 분포하며, 이배체 종으로 구성된 층층나무과에 속한다,층층나무속 7종의 계통관계와 유전적 다양성을 분석하기 위하여 RAPD marker를 사용하였다. 속들 간의 유전적 다양도는 0127부터 0180까지 평균 0.163을 나타내어 층층나무속들 간의 유전적 다양도가 높게 나타났다. 층층나우속의 유전적 다양도의 평균은 유사한 생활사의 특징을 가진 종들의 평균값보다 높았다. 이는 다년생이면서 유정생식을 하는 특징이 높은 식물의 유전적 다양도를 추정하는 요소이다. 전체 Nm값은 1.031로 한국산 층층나무 속들 사이에서 유전적 부동의 효과는 미미하였다. 계통수는 전혀 다른 3개의 clade를 보여주었다. 산수유와 산딸나무가 한 clade를 형성하였고, 풀산 딸나무는 독립적 분지를 나타내었으며 나머지 층층나무, 곰의말채, 말채나무, 흰말채나무가 한 clade를 형성하면서 산수유와 산딸나무의 clade와 sister group을 나타내었다. 이러한 계통수는 한국산 층층나무 속의 유전적 다양성을 보여주었다. Comas is a genus of the family Comaceae consists of diploid species (2n=18, 20, 22) and is mainly distributed in northeastern Asia. Random Amplified Polymorphic DNA(RAPD) markers were performed in order to analyse the phylogenetic relationships of seven species of C canadensis, Comas alda, C. controuersa, C macrophylla, C waited, C officinalis, and C kousa & And also used to investigate the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.163 across species, varying from 0.127 to 0.180. A high level of genetic variation was found in populations of Comas species Specially, Nei`s gene diversity for C walteri was maintained high. Mean of genetic diversity in Comas species was higher than average values for species with similar life history traits. The sexual reproduction, perennial habitat, and longevity are proposed as possible factors contributing to high genetic diversity. An indirect estimate of the number of migrants per generation (Nm=1.031) indicated that gene flow was extensive among Korean populations of Cornus species. The phylogenic tree showed distinct three clades. One included C officinalis and C kousa and another included four Comas species, Calba, C. macrophylla, C controversa and C walteri. The C canadensis showed sister group of both clades, the tree also showed genetic differentiation among Korean species.

한국산 돌콩 자연집단의 유전적 다양성

정수동(Su Dong Chung),허홍욱(Hong Wook Huh),정명기(Myong Gi Chung) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.1

N/A

Genetic and Phylogenetic Relationships of Genus Hemerocallis in Korea Using ISSR

Joo Soo Choi(최주수),Hong Wook Huh(허홍욱),Seol-A Lee(이설아),Man Kyu Huh(허만규) 한국생명과학회 2008 생명과학회지 Vol.18 No.6

원추리속(Genus Hemerocallis) 식물은 초본이며 일부 종은 약용으로 매우 중요하다. 이 속내 8개 분류군에 대해 ISSR (inter simple sequence repeats) 마커로 계통학적 관계를 분석하였다. 조사한 식물은 원추리(Hemerocallis fulva), 왕원추리(H. fulva for. kwanso), 각시원추리(H. dumortieri), 골잎원추리(H. coreana), 홍도원추리(H. hongdoensis), 큰원추리(H. middendorffi), 노랑원추리(H. thunbergii), 애기원추리(H. minor)이다. 또한 이들 분류군에 대한 유전적 변이와 구조를 조사하였다. 종간 유전적 다양도는 0.068~0.123이며 평균 유전적 다양도는 0.098로 전반적으로 낮았다. 애기 원추리가 가장 높은 값을 나타내었다. 세대 당 이주하는 개체수는 매우 적었다(Nm=0.218). 원추리속 종은 계통도에서 세 분지군으로 나누어졌다. 한 그룹은 H. fulva, H. fulva for. kwanso, H. middendorffi였다. 다른 그룹은 Hemerocallis, H. dumortieri, H. thunbergii, H. minor였다. 나머지는 H. coreana와 H. hongdoensis로 두 번째 그룹과 자매군을 형성하였다. 비록 종 내 적은 개체수로 분석하였지만 원추리속 식물종이 ISSR 마커로 잘 분리되었다. Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm=0.218) indicated that gene flow was not extensive among Korean populations of Hemerocallis species. The phylogenic tree showed distinct three clades. One includes H. fulva, H. fulva for. kwanso and H. middendorffi. Another includes three Hemerocallis species, H. dumortieri, H. thunbergii and H. minor. The H. coreana and H. hongdoensis were shown as the sister group to the second clades. Although the size of sampling was not large enough for eight Korean Hemerocallis species, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of species.

ITS 서열을 이용한 조팝나무속의 계통분류학적 연구

허만규(Man Kyu Huh),허홍욱(Hong Wook Huh),이소영(So Young Lee) 한국생명과학회 2008 생명과학회지 Vol.18 No.5

Genus Spiraea is a long lived woody species that is primarily distributed throughout Asia and Europe. We evaluated a representative sample of the fourteen taxa in Korea with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within genus. The molecular data allowed us to resolve well-supported clades in taxa. Aligned nucleotide sequences of the length of ITS1 were nearly constant within genus Spiraea varying from 237 to 238. However, S. pubescens and S. salicifolia were showed 227 bp and 242 bp, respectively. Especially, the 5.8S subunit of all taxa of Spiraea was found to 157-165 bp nucleotides. However, aligned nucleotide sequences of the length of ITS2 vary from 263 to 271 bp. Total alignment length is 670 positions, of which 65 are parsimony-informative, 45 variable but parsimony-uninformative, and 410 constant characters. The phylogenic tree showed Korean taxa of genus Spiraea were well separated each other. S. prunifolia for. simpliciflora and S. blumei are conform one group and S. chartacea is the sister to them. S. fritschiana and S. S. miyabei are conform one group and S. japonica is the sister to them. S. pubescens was recognized as a distinct species.