독서사회학의 관점에서 본 독자

허경은(HEO Kyeong-Eun) 프랑스학회 2000 프랑스학연구 Vol.19 No.-

독활지황탕가미방을 포함한 한의치료로 자율신경장애가 호전된 MSA-C 환자에 대한 치험 1례

허경화,김동주,허혜민,황예채,조승연,박정미,고창남,박성욱,Kyeong-Hwa Heo,Dong-Joo Kim,Hye-Min Heo,Ye-Chae Hwang,Seung-Yeon Cho,Jung-Mi Park,Chang-Nam Ko,Seong-Uk Park 대한중풍순환신경학회 2023 대한중풍.순환신경학회지 Vol.24 No.1

■Objectives This study is to report the effectiveness of Korean medicine on the treatment for multiple system atropy(MSA-C) patient complaining of autonomic dysfunction. ■Methods The patient was treated with Korean medicine(mainly DokhwalJihwang-tang Gami-bang) for 39 days. The evaluations were performed using UMSARS(Unified Multiple System Atrophy Rating Scale), SCOPA-AUT(The Scale for Outcomes in Parkinson's disease-Autonomic), K-OGS(Korean version of the Orthostatic Grading Scale). ■Results After treatment, the UMSARS score decreased from 25 to 18, the SCOPA-AUT score decreased from 21 to 14, K-OGS score decrease from 15 to 8. ■Conclusion This case suggests that Korean medicine treatment may be effective for MSA-C patients with autonomic dysfuction.

3차원 보행분석기를 통해 보행장애의 호전이 확인된 비정형 파킨슨증후군 환자 한방치험 1례

허혜민,이경화,허경화,황예채,조승연,박정미,고창남,박성욱,Hye-Min Heo,Kyeong-Hwa Lee,Kyeong-Hwa Heo,Ye-Chae Hwang,Seung-Yeon Cho,Jung-Mi Park,Chang-Nam Ko,Seong-Uk Park 대한중풍순환신경학회 2023 대한중풍.순환신경학회지 Vol.24 No.1

■Objectives This case study is to report the effects of Korean medicine on parkinsonism patient's Gait Disturbance. ■Methods During 12 days of hospitalization, the patient was treated by acupuncture, pharmaco-acupuncture, moxibustion, herbal medicine, especially Cheongsimyeonjatang-gamibang. In order to assess the change of symptoms, we used a 3-Dimensional(3D) gait analysis system, Unified Parkinson's Disease Rating Scale(UPDRS), analysis of gait video and self-evaluation of discomfort. ■Results After treatment, The improvements of walking pattern were observed in both objective analysis results of gait analysis system and subjective video analysis. And the UPDRS score decreased, especially Part III score decreased more than minimal clinically important difference(MCID). In addition, There was improvement in self assessment of the patient. ■Conclusion This study suggests that Korean medical treatment might be effective in motor disorder of parkinsonism patient.

HPLC를 이용한 Isoproturon, Phenmedipham, Pyridate 및 Nitenpyram 4종 성분의 잔류농약 분석법 개발

양성용(Sung-Yong Yang),구윤창(Yun-Chang Koo),Zeng Wang,허경(Kyeong Heo),김형국(Hyeongkook Kim),안은미(Eun-Mi An),신한승(Han-Seung Shin),이진원(Jin-Won Lee),이광원(Kwang-Won Lee) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.8

Bis-carbamate계(phenmedipham), urea계(isoproturon), thiocarbamate계(pyridate) 및 vinyllidenediamine계(nitenlyram) 4종의 농약을 HPLC를 이용하여 분석하는 방법을 개발하였다. 사용된 칼럼은 C-18(250 ㎜×4.6 ㎜, 5 ㎛ diameter particle size)이며 isoproturon, phenmedipham은 acetonitrile과 물을 50:50으로 섞어서 사용하였으며, pyridate는 acetonitrile과 물을 85:15로, nitenpyram은 phosphoric acid를 이용하여 pH 2.5로 맞춘 50 mM KH₂PO₄와 acetonitrile을 90:10으로 섞어 유속 1 mL/min의 isocratic 조건으로 분석하였다. 시료 주입량은 10 μL이며 retention time은 isoproturon 6.12분, phenmedipham 8.63분, pyridate 9.40분, nitenpyram 12.76분, 정량한계는 모두 0.05 ㎎/㎏이었다. 회수율 실험은 쌀, 사과 및 대두에 4종의 농약 표준품을 각각 0.05, 0.1 및 0.5 ㎎/㎏이 되도록 세 가지 농도로 spiking 하였으며, 회수율 및 분석오차는 70.18~118.08% 범위와 10% 이내를 만족하였다. A method for the determination of four pesticide compounds, urea (isoproturon), bis-carbamate (phenmedipham), thiocarbamate (pyridate) and vinyllidenediamine (nitenpyram) were examined and analyzed by HPLC with C-18 column (250 ㎜×4.6 ㎜, 5 ㎛ diameter particle size). Mobile phase consisted of deionized water, acetonitrile and 50 mM KH₂PO₄ (pH 2.5). Isoproturon and phenmedipham analytical condition was isocratic elution of the column with 50% solvent A (acetonitrile) and 50% solvent B (deionized water); pyridate was 85% solvent A (acetonitrile) and 15% solvent B (deionized water) at a flow rate of 1 mL/min; and nitenpyram analytical condition was 90% solvent A (50 mM KH₂PO₄, pH 2.5) and 10% solvent B (acetonitrile) at a flow rate of 1 mL/min. In results, retention times were 6.12, 8.63, 9.40 and 12.76 min for isoproturon, phenmedipham, pyridate and nitenpyram, respectively. All injection volumes were 10 μL and the limit of quantitation was 0.05 ㎎/㎏ for four pesticide compounds, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four pesticide compounds were spiked at concentrations of 0.05, 0.1 and 0.5 ㎎/㎏. The recovery rates were ranged from 70.18% to 118.08% and the standard deviations of all experiments were within 10%.

Lactobacillus acidophilus 88의 Protoplast 형성 및 재생에 관한 연구

전홍기,허경,조영배,백형석 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.2

유산균 육종의 일환으로 protoplast fusion 방법을 사용하여 Lactobacillus casei KCTC 1121과 Lactobacillus acidophilus 88을 융합시키기 위해 먼저 L. acidophilus 88의 원형질체 형성 및 재생 조건을 조사하였다. L. acidophilus 88은 다양한 indicator 균들에 대해 강한 생육저지효과를 나타내는 bacteriocin을 생산하였으나, L. casei는 bacteriocin 생성능이 거의 없었다. 융합시 유전적인 선별 marker를 부여하기 위하여 streptomycin(2 ㎎/㎖)에 내성을 가진 L. casei와 kanamycin(600 ㎍/㎖)에 대해 내성을 나타내는 L. acidophilus 88을 분리하였다. L. acidophilus 88은 대수증식기 중반에서 후반에 원형질체가 가장 잘 형성되었고, lysozyme 125 ㎍/㎖를 원형질체 형성용 완충액에서 37℃, 30분간 처리했을 때 원형질체 형성 및 재생율이 가장 좋았으나, mutanolysine에 대해서는 감수성이 적었다. 원형질체 형성용 완충액은 20 mM HEPES buffor(pH 7.0)에 삼투압 안정제로 sucrose 1 M을 가했을 때 가장 양호하였으며, MgCl_2는 원형질체 형성에 좋지 않았다. Protoplast 재생은 재생용 배지에 10%의 sucrose를 첨가했을 때 가장 효과적이었다. In the course of the study on strain improvement by protoplast fusion, Lactobacillus acidophilus 88 protoplasts production and regeneration conditions were investigated. This strain produced a bacteriocin that revealed strong inhibitory activity against various indicator strains, especially L. helveticus CNRZ 1096. Protoplasts of L. acidophilus 88 strains were very efficiently obtained by treatment with 125 ㎍/㎖ lysozyme in a protoplast forming buffer containing 20 mM obtained by treatment with 125 ㎍/㎖ lysozyme in a protoplast forming buffer containing 20 mM N-2-hydroxy-ethyl-piperazine-N’-2-ethane-sulfonic acid (HEPES, pH 7.0) and 1 M sucrose at 37℃ for min. However, treatment with mutanolysin was not effective for the production of L. acidophilus 88 protoplasts under the same conditions. High protoplast yield was obtained from the cells at the middle to late logarithmic growth phase in the de Man, Rogosa and Sharpe(MRS) medium. Regeneration was efficiently accomplished with the MRS medium containing 10% sucrose.

5-Fluorouracil-1-yl-3-butanone의 항암제 내성 극복에 관한 연구

서보정,허경,강치덕,김선희,정병선,김정균 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-

암화학요법에 대한 암세포의 약제내성을 극복하기 위한 하나의 방법으로 항암제와 그 유사체의 병용에 의한 항종양 활성의 향상이 시도되고 있다. 5-FU는 유암, 자궁암,위암 등에 사용되고 있는 fluorinated pyrimidine계 항암제이나, 암세포의 5-FU에 대한 내성이 치료에 장애가 되고 있다. 5-FU의 항종양 활성의 향상과 내성극복을 위하여 5종의 5-FU 유도체에 대하여 MTT방법으로 5-FU와의 병용에 의하여 5-FU의 활성증강을 시키는 물질을 조사한 바, 신합성물질인 5-Fouirouracil-1-yl-3-butanone(5-FUBO)에 의한 5-FU의 세포독성 증강효과가 5-FU에 감수성인 암세포에서 보다 내성인 암세포에서 병용효과가 높았으며, 5-FU뿐만아니라 임상적으로 사용되고 있는 항암제인 VCR, VBL과의 병용효과를 나타내는 흥미있는 현상을 발견하였다. 이 합성물질에 의한 VCR 내성극복은 항암제 다제내성세포를 사용하여 조사한 바, VCR과 상기의 합성물질인 5-FUBO(o.1㎍/㎖)의 병용에 의한 VCR 내성극복 정도는 다제내성도가 낮은 암세포에서는 2.4배를 나타내었으며 다제내성도가 높은 세포에서는 11배로서 그 효과가 현저히 높은 것으로 확인하였다. 이와 같이 5-FUBO는 5-FU뿐만 아니라 vinca alkaloid계 항암제인 VCR, VBL과의 병용효과를 나타내는 매우 흥미있는 합성물질로서, 이 물질이 항암제 다제내성 변이주에서 발현하는 MDR1 유전자의 발현을 농도의존적으로 억제하였다. The potentiation effect of anticancer agents by their analogues is one of the possible strstergies for overcoming cellular resistance to chemotherapy. 5-Fouirouracil(5-FU)is a fluorinated pyrimidine which has been used in the treatment of gastrointestinal, breast and ovarian cancers. In attempts to improve the efficasy of 5-FU and overcome 5-FU resistance, We enaluated the sensitizing effect om 5-FU-induced cytotoxicity of various newly synthesized 5-FU derivatives by means of a colormetric MTT assay. 5-Fouirouracil-1-yl-3-butanone(5-FUBO), a newly synthesized 5-FU derivative, potentiates the cytotoxicity of 5-FU in 5-FU resistant mutant to a greater extent than drug-sensitive parental cells. Also, it potentiates the cytotoxic activity of clinically useful antitumor agents such as vincristine(VCR) and vinblastine(VBL). Overcoming by VCR resistance by 5-FUBO was investigated by using multidrug resistant (MDR) cells, VCR cytotoxicity was potentiated 2.4 fold in low-grade MDR cells compared with parental cells by 5-FUBO at o.1㎍/㎖ . In MDR P388(P388/M) cells, 5-FUBO gene inhibited the epression of MDR1 gene in dose-dependent manner. It may be noteworthy the cytotoxicity of structurally different anticancer agents were more potentiated drug resistant mutant to a greater extent than the parental cells.