http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
LMK02의 품질규격화와 $A{\beta}$ 올리고머에 의해 유도된 희주해마 H19-7세포주에 미치는 항치매효과
강형원,김상태,손형진,한평림,조형권,이영재,류영수,Kang, Hyung-Won,Kim, Sang-Tae,Son, Hyeong-Jin,Han, Pyeong-Leem,Cho, Hyoung-Kwon,Lee, Young-Jae,Lyu, Yeoung-Su 대한동의생리학회 2009 동의생리병리학회지 Vol.23 No.2
For standardization of LMK02 quality, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelica gigas Nakai in the constituents of LMK02 were estimated as indicative components. From LMK02 water extract, has been used in vitro test for its beneficial effects on neuronal survival and neuroprotective functions, particularly in connection with APP-related dementias and Alzheimer's disease (AD). $A{\beta}$ oligomer derived from proteolytic processing of the ${\beta}$-amyloid precursor protein (APP), including the amyloid-${\beta}$ peptide ($A{\beta}$), play a critical role in the pathogenesis of Alzheimer's dementia. We determined that oligomer amyloid-${\beta}$ ($A{\beta}$) have a profound attenuation in the increase in rat hippocampus H19-7 cells from. Experimental evidence indicates that LMK02 protects against neuronal damage from cells, but its cellular and molecular mechanisms remain unknown. Using a hippocampus cell line on $A{\beta}$ oligomer-induced neuronal cytotoxicity, we demonstrated that LMK02 inhibits formation of $A{\beta}$ oligomer, which are the behavior, and possibly causative, feature of AD. In the Red Ginseng, the average amounts of Ginsenoside Rg3 were $47.04{\mu}g/g$ and $42.3{\mu}g/g$, 90 % of its weight were set as a standard value. And, in the Angelica gigas Nakai, the average amounts of Decursin were 2.71 mg/g and 2.44mg/g, 90 % of its weight were also set as a standard value. The attenuated $A{\beta}$ oligomer in the presence of LMK02 was observed in the conditioned medium of this $A{\beta}$ oligomer-induced cells under in vitro. In the cells, LMK02 significantly activated antiapoptosis and decreased the production of ROS. These results suggest that neuronal damage in AD might be due to two factors: a direct $A{\beta}$ oligomer toxicity and multiple cellular and molecular neuroprotective mechanisms, including attenuation of apoptosis and direct inhibition of $A{\beta}$ oligomer, underlie the neuroprotective effects of LMK02 treatment.
장원환가감방(壯元丸加減方)인 LMK02가 아밀로이드 전구단백질(前驅蛋白質)으로 형질전환(形質轉換)된 초파리에 미치는 효과
김상태 ( Sang Tae Kim ),강형원 ( Hyung Won Kang ),한평림 ( Pyeong Leem Han ),조형권 ( Hyoung Kwon Cho ),김태헌 ( Tae Heon Kim ),류영수 ( Yeoung Su Lyu ),손형진 ( Hyeong Jin Son ) 대한한방신경정신과학회 2008 동의신경정신과학회지 Vol.19 No.2
Objective : Recent studies indicate that the deposition of beta-amyloid (Aβ) is related in the pathogenesis of Alzheimer`s disease (AD), but the underlying mechanism is still not clear. Method : To investigate the potential cellular functions of APP and LMK02, we use transgenic drosophila as a model was treated with either LMK02, and the effect in APP expression was determined by climbing assay. LMK02 have been shown to be neuroprotective in fly model systems. We asked whether dietary supplementation with LMK02 would influence behavior and AD-like pathology in a transgenic fly model. Result : LMK02 water extract have attenuated fly death in vivo. LMK02-treated fly increased percentage of flight ability more longly and survival ratio more than controls. APP-GRIM drosophila treated with LMK02 had significantly less accumulation of APP deposition in the eye and brain as compared to control drosophila. Conclusion : These results suggest that LMK02 prevent APP-induced neurotoxicity through attenuating flies death induced by APP, and may be useful as potential therapeutic agents for AD.
장원환가감방(壯元丸加減方) 수추출물(水抽出物)이 아밀로이드 전구단백질으로 유도된 생쥐의 신경아세포주에서의 항치매 효과
강형원 ( Hyung Won Kang ),김상태 ( Sang Tae Kim ),이종화 ( Jong Hwa Lee ),김태헌 ( Tae Hyeon Kim ),손형진 ( Hyeong Jin Son ),한평림 ( Pyeong Leem Han ),류영수 ( Yeoung Su Lyu ) 대한한방신경정신과학회 2007 동의신경정신과학회지 Vol.18 No.2
Objective : Recent studies indicate that the deposition of β-amyloid (Aβ) is related in the pathogenesis of Alzheimer`s disease (AD), but the underlying mechanism is still not clear.Method : To investigate the potential cellular functions of APP and water extract of the JangwonHwangagambang (JWHG), we use as in vitro model, neuro 2A cells were treated with either JWHG or its oriental medicines, and the effect in APP expression was determined by MTT and LDH assay. JWHG have been shown to be neuroprotective in different model systems. We asked whether JWHG treatment would influence cell survival and AD-like pathology in APP-induced neuronal cells.Result : JWHG and water extracts of some oriental medicine has attenuated high cell death in vitro. JWHG-treated cells increased percentage of cell survival more longly than controls. JWHG had significantly increas neurite outgrowth in the as compared to control cells. Conclusion : These results suggest that JWHG prevent APP-induced neurotoxicity through attenuating oxidative stress, and may be useful as potential therapeutic agents for AD.