자궁경부암의 근치적 절제술 후 총장골동맥림프절 침범 시 동시항암화학치료와 예방적 대동맥주위림프절 방사선조사의 효과

한태진(Tae Jin Han),우홍균(Hong-Gyun Wu),김학재(Hak Jae Kim),하성환(Sung Whan Ha),강순범(Soon-Beom Kang),송용상(Yong-Sang Song),박노현(Noh-Hyun Park) 대한방사선종양학회 2010 Radiation Oncology Journal Vol.28 No.3

목 적: 자궁경부암에서 근치적 절제술 후 림프절 침범은 중요한 예후인자 중 하나이다. 본 연구에서는 총장골동맥림프절 침범 시 대동맥주위림프절에 대한 예방적 방사선조사의 효과를 알아보고자 하였다. 대상 및 방법: 1985년 5월부터 2004년 10월까지 총 909명의 환자가 서울대병원에서 자궁경부암으로 근치적 절제술 후 방사선치료를 시행받았다. 골반 내 림프절 침범이 있는 환자는 375명이었고 총장골동맥림프절 침범이 있는 환자는 69명이었다. 이 중 총장골동맥림프절 침범이 있으면서 대동맥주위림프절 침범은 없었던 54명의 환자를 대상으로 후향적 분석을 시행하였다. 대상환자의 FIGO 병기는 IB, IIA, IIB가 각각 22명, 21명, 11명이었다. 이 중 10명은 전골반과 대동맥주위림프절을 포함하는 확장조사야로 방사선치료를 받았고 모두 항암화학치료를 병용하였으며, 나머지 44명은 전골반 만을 포함하는 표준조사야로 방사선치료를 받았고 이 중 16명이 항암화학치료를 병용하였다. 확장조사군과 표준조사군의 추적관찰기간은 각각 21∼58개월(중간값, 47개월)과 6∼201개월(중간값, 58개월)이었다. 결 과: 전체 환자의 4년 생존율, 4년 무병생존율, 4년 무전이생존율은 각각 70.0%, 61.1%, 71.7%였다. 단변량 분석 시 절제연 침범(p<0.001), 림프관내 종양 침범(p=0.041)이 있는 경우 유의하게 생존율이 낮았고, 양측 림프절 침범(p=0.001), 5개 이상의 림프절 전이(p=0.006)가 있는 경우 유의하게 낮은 무병생존율을 보여주었다. 낮은 무전이생존율과 관련 있는 인자는 양측 림프절 침범(p=0.009), 5개 이상의 림프절 전이(p=0.003), 자궁경부 전층 침범(p=0.013), 절제연 침범(p=0.014), 림프관내 종양 침범(p=0.041)이었다. 확장조사군과 표준조사군의 4년 생존율은 90.0%와 67.2% (p=0291), 4년 무병생존율은 70.0%와 59.0% (p=0.568), 4년 무전이생존율은 90.0%와 67.5% (p=0.196), 4년 대동맥주위림프절 전이율은 0%와 14.3% (p=0.249)로 통계적으로 유의한 차이가 없었다. 3등급 이상의 중등도 급성합병증은 확장조사군 10명 중 4명(40%; 조혈계 2명, 위장관계 2명)에서 발생하였고, 표준조사군 44명 중에서는 11명(25%; 조혈계 2명, 위장관계 6명, 비뇨생식계 3명)에서 발생하였다. 결 론: 자궁경부암의 근치적 절제술 후 총장골동맥림프절 침범이 있는 경우 예방적 대동맥주위림프절 방사선조사의 생존율에 대한 통계적 유용성은 확인할 수 없었다. 그러나 확장조사야로 치료받은 환자수가 적고 추적관찰기관이 짧았던 점을 고려하면 추가적인 추적관찰이 필요할 것으로 생각한다. Purpose: To retrospectively assess the advantages and side effects of prophylactic Paraaortic irradiation in cervical cancer patients with common iliac nodal involvement, the results for survival, patterns of failure, and treatment-related toxicity. Materials and Methods: From May 1985 to October 2004, 909 patients with cervical carcinoma received postoperative radiotherapy at the Seoul National University Hospital. Among them, 54 patients with positive common iliac nodes on pathology and negative Paraaortic node were included in the study. In addition, 44 patients received standard pelvic irradiation delivered 50.4 Gy per 28 fractions (standard irradiation group), and chemotherapy was combined in 16 of them. The other 10 patients received pelvic irradiation at a dose of 50.4 Gy per 28 fractions in addition to Paraaortic irradiation at 45 Gy per 25 fractions (extended irradiation group). In addition, all of them received chemotherapy in combination with radiation. Follow-up times for pelvic and Paraaortic irradiation ranged from 6 to 201 months (median follow-up time, 58 months) and 21 to 58 months (median follow-up time, 47 months), respectively. Results: The 4-year overall survival, disease free survival, and distant metastasis free survival in the standard irradiation group and extended irradiation group were 67.2% vs. 90.0% (p=0.291), 59.0% vs. 70.0% (p=0.568) and 67.5% vs. 90.0% (p=0.196), respectively. The most common site of first failure for the standard irradiation group was the paraaortic lymph node, while no paraaortic failure was observed in the extended irradiation group. Relatively, hematologic toxicity grade 3 or greater was common in the extended irradiation group (2/10 extended vs. 2/44 standard), while gastrointestinal toxicity of grade 3 or greater was lower (2/10 extended vs. 6/44 standard), and urologic toxicity of grade 3 or greater was observed in the standard irradition group only (0/10 vs. 3/44). Conclusion: Concurrent chemotherapy and prophylactic Paraaortic irradiation in patients with common iliac nodal involvement showed slightly improved clinical outcomes aside from increased hematologic toxicity, which was statistically insignificant. Considering the relatively small number of patients and short follow-up times, additional studies are needed to obtain more conclusive outcomes.

공기업 CEO의 리더십특성이 조직구성원의 리더신뢰수준과 조직유효성에 미치는 영향

한태진(Tae-Jin Han),김홍(Hong Kim) 한국콘텐츠학회 2008 한국콘텐츠학회논문지 Vol.8 No.11

본 연구는 공기업 CEO의 리더십특성이 조직구성원의 리더신뢰수준과 조직유효성에 미치는 영향을 시장형과 준시장형 공기업 14개회사를 중심으로 1,124명의 표본을 대상으로 조사하였는데 조사결과는 다음과 같다. ⓛ 리더의 개인적 배려가 신뢰수준에 영향을 미치고, ② 변혁적 리더십과 거래적 리더십이 조직 유효성에 영향을 미치는 것으로 분석되어 가설이 채택되었다. 그리고 ③ 리더의 신뢰수준으로서의 계산적, 지식적, 동일화 요인이 조직 유효성의 직무만족과 조직몰입에 긍정적인 영향을 높이는 기제로 나타났으나, 신뢰수준에서 계산적 변수의 조직몰입은 기각되었다. 전체적으로 공기업 CEO 리더십 특성으로서의 변혁적, 거래적 리더십이 계산적, 지식적, 동일화 정도의 상호신뢰수준에 따른 조직 유효성에 미치는 영향검증 결과 동일화 기반에서의 조직몰입은 기각되고 그 외 요인은 긍정적인 기제로 채택되어 공기업 CEO의 리더십 특성에 적합한 기제임을 입증시켜 주었다. The purpose of this study is to inquire into the effect of characteristics of public enterprise CEOs' leadership (transforming leadership and transactional leadership) on the leader trust and organizational effectiveness of organizational members. A survey was made on 1,124 persons being in the service of 14 marketable and quasimarketable public enterprises. The results of this study were as follows. First, it showed that the individualized consideration had an effect on the confidence level of leader. Second, it showed that the transforming and transactional leaderships had an effect on the organizational effectiveness, which the hypothesis was adopted. Third, it showed that calculable, intellectual and identical factors had a positive effect on the job satisfaction and organizational commitment, but the organizational commitment of calculable variable was rejected in the confidence level. In general, the effect of transforming and transactional leaderships as public enterprise CEOs' leadership characteristics on the organizational effectiveness by mutual confidence level of calculable, intellectual and identical standards showed that the organizational commitment was rejected in the base of identification. And other factors were adopted as a positive mechanism, which they were a mechanism suitable to public enterprise CEOs' leadership characteristics.

C형 간염 항체 검출을 위한 새로운 제3세대 전자동 미세 입자 효소면역측정법의 평가

한태진 ( Tae Jin Han ),서숙원 ( Suk Won Seo ),문애란 ( Ae Ran Moon ),오원일 ( Won Il Oh ) 대한임상검사과학회 1997 대한임상검사과학회지(KJCLS) Vol.29 No.1

서울중앙병원에서 IMx를 이용한 혈장내 총Homocysteine의 측정

한태진 ( Tae Jin Han ),신연수 ( Yeun Soo Shin ),유승용 ( Seung Yong Yu ),장진연 ( Jin Yeon Jang ),조병철 ( Byoung Cheol Cho ),전사일 ( Sa Il Chun ),민원기 ( Won Ki Min ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Background : Elevated plasma homocysteine (HCY) levels are considered to be an independent risk factor in coronary 따tery disease, cerebrovascular disease, and peripheral vascular disease. Currently HPLC fluoremetric detection has been most widely used for the quantitation of homocysteine. Recently IMx homocysteine assay by use of a fluorescence polarization immlUloassay(FPIA) has been introduced that enables rapid quantitation of plasma homocysteine levels. We evaluated the performance of the IMx@ Homocysteine assay and compared it to routinely used HPLC method. Methods Within-run precision, between-run precision, degree of correlation, linearity, and reportable ranges were analyzed, according to National Committee for Clinical Laboratory Standarili잉 guidelines as much as possible. To establish a reference interval 148 apparently healthy adults were investigated (81 males, 67 females). For the methods comparison We selected 20 patients admitted to the Asan Medical Center. The total plasma homocysteine concentraton was measure by FPIA (lMx""J Homocysteine assay, Abbotte Laboratories, IL, USA) and by HPLC (HPll00, Hewlett Packard com., Germany) using Bio-rad@ Homocysteine kit (Bio-Rad Laboratories, CA, USA). The caIculations were performed with computer software, EP Suit(MarChem Associates, Concord, USA). Result : The within run coefficients of variations (CVs) of the low, medium, and of the high control were 1.8%, 1.5% and 1.5%. The between run CVs were 2.8%, 1.4% and 1.0%, respectively. Method was linear and passed lack of fit. Reportable range was found to be 0.5-50.0 μ molfL. The reference interval from hea1thy adu1ts was 10.3 i: 5.54(mean i: SD) μ molfL. Total plasma homocysteine levels according to sex were 11.9 i: 6. 89(mean i: SD) μ molfL for male, 8.39i: 2.03(meani: SD) μ mol/L for female, respectively. The comparison between FPIA (y) and HPLC(x) resu1ted in a linear regression analysis described by the equation : y=0.9463x-0.2364 with a correlation coefficient r=0.9961 Conclusions Linearity, pricision, reportable ranges of the IMx homocysteine were satisfactory. The IMx homocysteine also correlated well with HPLC method. The normal range, determined by testing apparent1y hea1thy adu1ts, is in agreement with the literature. πle IMx homocysteine assay is fast and easy to perform and very useful for routine determinations of plasma homocysteine levels.

Cymbidium sp. Protocorm 의 묘조분화시 냉생 Polyamine 함량의 변화

한태진(Tae Jin Han),강영희(Young Hee Kang),김성호(Sung Ho Kim) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.1

Changes in polyamine titers during shoot differentiation in Cymbidium sp. (Jungfrau) protocorms were studied in order to investigate the mechanism of shoot differentiation by using auxin-inhibitors(PCIB, TIBA), hormones(GA_3, ABA, BA), and phenolic compounds (2,4-dichlorophenol, catechol). The shoot differentation and propagation of protocorms were promoted by PCIB or 2,4-dichlorophenol, and the growth of differentiated shoot were promoted by TIBA or catechol. In BA-treated protocorms, white or brown protocorms were observed. Putrescine was the most abundant polyamine during the propagation and differentiation processes. As compared with putrescine, spermidine did not show significant changes and spermine was not detected at all. Putrescine titers decreased after a temporary increase, and then again increased in the presence of GA_3, ABA, 2,4-dichlorophenol, and then again increased in the presence of GA_3, ABA, 2,4-dichlorophenol, catechol, or PCIB. But, in BA-treated protocorms, putrescine level was much lower than spermidine.

애기장대(Arabidopsis thallana) 잎 절편에서의 기관형성 특히 Naphthale neacetic acid의 농도에 따른 부정근, 모용 및 캘러스 형성과 Determination Time

한태진(Han Tae Jin),김인현(Kim In Hyeon),김송림(Kim Song Lim),김준철(Kim Joon Chul),임창진(Lim Chang Jin),진창덕(Jin Chang Duck) 강원대학교 기초과학연구소 1999 기초과학연구 Vol.10 No.-

The effect of auxins and cytokinins on the formation of adventitious shoots, adventitious roots, trichomes, and calli in MS basal medium was investigated in leaf segments from ecotype Columbia of Arabidopsis thallana. Adventitious shoots, adventitious roots, trichome, and calli were formed from leaf segments by a wide range of hormone concentrations and combinations. Adventitious shoots were formed respectively in treatment with 0.1 mg/L IAA and 10 mg/L BA. Adventitious roots were formed in treatments with low concentration of IAA and NAA. Trichomes and calli were formed by increasing the concentration of IAA and NAA. The optimal combination was 0.5 mg/L NAA and 0.1 mg/L BA for trichome formation, 10 mg/L NAA and 10 mg/L BA for calli formation. When NAA was treated alone in culture media, adventitious roots were formed in 0.1 mg/L, trichomes were formed in 2.0 mg/L, and calli were formed in 1.0 mg/L Inductive time for formation of adventitious roots, trichomes and calli were determined at 6,7 and 18 days respectively by periodical transfer of leaf segments from NAA containing medium to NAA free medium. Key words: Adventitious shoot, Iaa kinetin, BA 2,4-D

Cymbidium spp. 의 Protocorm 묘조 분화시 Protocorm 내 전분 함량에 미치는 GA3 와 ABA 의 영향

한태진(Tae Jin Han),강영희(Young Hee Kang),김응식(Eung Sik Kim) 한국식물학회 1988 Journal of Plant Biology Vol.31 No.4

Changes in starch content and activities of ADPG-and UDPG-starch synthase and α-and, β-amylase were studied in order to investigate effects of gibberellic acid and abscisic acid on endogenous starch content during shoot differentiation and protocorm propagation in Cymbidium spp. (Jungfrau) protocorm. Shoot differentiation was promoted during the degradation of endogenous starch and protocorm propagation was promoted during starch accumulation in protocorm. The activities of ADPG-and, UDPG-starch synthase and α-and, β-amylase seemed to be related with starch content. Shoot differentiation and protocorm propagation were slightly inhibited in protocorm explants treated with 100 μM gibberellic acid. The explants treated with 10 μM abscisic acid lost the capacity for shoot differentiation and protocorm propagation, and that could not be overcome by 100μM gibberellic acid added to culture medium. Starch content fluctuated as the control even after 10 μM abscisic acid. None the less, the treatment completely inhibited shoot differentiation and protocorm propagation.

서울중앙병원에서 시행된 항핵항체와 가용성 향원 겸샤 결과의 비교 분석

한태진 ( Tae Jin Han ),조영욱 ( Young Uk Cho ),김봉철 ( Bong Cheol Kim ),조병철 ( Byung Chul Cho ),정윤영 ( Yoon Young Chung ),오흥범 ( Heung Bum Oh ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2

Background : Antinuclear antibodies(ANA) have high sensitivity for the diagnosis of systemic rheumatologic diseases, but have low specificiη relatively. Recently, we have used antibodies to extractable nuclear antigens(ENA) additiona11y, which enabled us to determine what the ANA detected rea11y were. The purpose of this study was to examine frequencies of autoantibodies and clinica1 significances of them by ana1yzing resu1ts of ANA, anti-ENA and anti-dsDNA tests. Methods: From Januaη, 1997 to March, 1998, the sera of 339 patients who were suspected that they have systemic rheumatologic diseases and 54 ones diagnozed as systemic lupus eηthematosus(SLE) were ana1yzed by performing ANA, anti-ENA(anti-RNAjSm, anti-SS - AjS S-B, anti-Sc170) and anti-dsDNA tests. Results : In 339 patients with suspicious systemic rheumatologic diseases, the concordance rate of positivity between ANA and anti-ENA was 23.6% and that of negativiη was 23.3%. The positivity rate of anti-ENA among patients with negative ANA was 0.9%. We detected anti-SS - AjRo in the rate of 41.0%, anti-RNP 16.9%, anti-Sm 2.4%, anti-SS - BjLa 1.2% and anti-Sc170 14.5% among patients with positive anti-ENA. Among 42 patients with positive anti-dsDNA, there were 32 cases with positive anti-ENA. 17 patients had anti-SS - AjRo, 4 ones anti-Scl70, 3 ones anti-RNPjSm. In 54 patients with SLE, the concordance rate of positivity between ANA and either anti-ENA or anti-dsDNA was 72.2%. Among patients with either positive anti-ENA or positive anti-dsDNA, we detected anti-SS - AjRo in the rate of 12.5%, anti-SS - Aj dsDNA 32.5% and anti-dsDNA 17.5%. Conclusion : When the titer of ANA is above 1: 160 and anti-ENA is negative, the accurate identification of ANA specificity would be essentia1 by using enzyme immunoassay or immunoblotting methods. The 1.9% positivity of anti-ENA among ANA negative SLE patients suggests the possibi1ity of presence of ANA-negative SLE in spite of performing ANA test using HEp-2 cells.

Cymbidium sp. 의 Protocorm 내 IAA 산화효소 활성변화가 묘조분화에 미치는 영향

한태진(Tae Jin Han),강영희(Young Hee Kang) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.2

Physiological gradient of IAA-oxidizing enzyme activities was investigated in order to elucidate the mechanism of shoot differentiation in Cymbidium sp.(`Jungfrau`) protocorms by using phenolic compounds (2,4-dichlorophenol, catechol), auxin-inhibitors (PCIB, TIBA), and hormones (GA_3, ABA, BA). The activity of IAA oxidase was decreased in protocorms treated with catechol decreased the catalytic activity of IAA oxidase or TIBA but this enzyme activity was increased after a temporary decrease at initial stages in the presence of 2,4-dichlorophenol or PCIB. The activity of IAA oxidase in BA-treated protocorms (white and crown gall-like) was the highest of all. However, the catalytic activity of peroxidase increased after a temporary decrease at initial period. These results suggest that shoot differentiation and growth may be influenced by effective IAA levels in the protocorms causing IAA-oxidizing enzymes and phenolic compounds.

애기장대(Arabidopsis thaliana)잎 절편에서 NAA와 BA에 의한 신초, 부정근, 모용 및 캘러스 형성 결정 시기

김송림,한태진,Kim, Song-Lim,Han, Tae-Jin 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

The effect of NAA and Benzyladenine(BA) for determination times on the formation of adventitious shoots, roots trichmoes and calli in MS basal medium was investigated in leaf segments from ecotype 'Nosses' of Arabidopsis thalliana. Adventitious shoots, roots, trichomes and calli were formed fromed from leaf segments in a wede range of NAA and BA. The optimal combination of hormones for adventitious shoots formation, 20mg/L NAA for trichome formation, 100mg/L for callus formation. Inductive times for formation of adventitious shoots, roots, trichomes and calli were determined at 14, 4, 6 and 18 days respectively by periodical transfer of leaf segments from hormines containing media to hormone free medium.