연체동물 유전체 연구현황

방인석,한연수,이준상,이용석,Bang, In-Seok,Han, Yeon-Soo,Lee, Jun-Sang,Lee, Yong-Seok 한국패류학회 2010 The Korean Journal of Malacology Vol.26 No.4

The availability of fast and inexpensive sequencing technology has enabled researchers around the world to conduct many genome sequencing and expressed sequence tag (EST) projects of diverse organisms. In recent years, whole genome projects have been undertaken to sequence ten species from the phylum Mollusca. These include Aplysia californica, Lottia gigantea, Crassostrea virginica, Spisula solidissima, Mytilus californianus, Biomphalaria glabrata, Crepidula fornicata, Elysia chlorotica, Lottia scutum and Radix balthica. Additionally, complete mitochondrial genomes of 91 mollusks have been reported. In Korea, EST projects have been conducted in nine mollusk species that include Nesiohelix samarangae, Pisidium (Neopisidium) coreanum, Physa acuta, Incilaria fruhstorferi, Meretrix lusoria, Ruditapes philippinarum, Nordotis gigantea, Crassostrea gigas and Laternula elliptica. Finally, the mitochondrial genome projects from the Pacific Oyster (Crassostrea gigas) and the rock shell (Thais clavigera) have been conducted and reported. However, no systemic mollusk genome project has so far been conducted in Korea. In this report, the current status and research trends in mollusk genome study in Korea will be discussed.

두둑을 재활용한 한국형 무경운 농업 III. 시설 무경운 유기재배 고추의 생육 및 생물다양성의 변화

양승구,신길호,김선국,김도익,한연수,정우진,Yang, Seung-Koo,Shin, Kil-Ho,Kim, Sun-Kook,Kim, Do-Ik,Han, Yeon-Soo,Jung, Woo-Jin 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.1

두둑을 재활용한 무경운 재배 토양의 고추의 생육량은 관행경운 토양에 비하여 22% 정도 증가되었고, 무경운 3년차는 12% 정도 증가되었다. 무경운 재배 2년차 토양에서 생산된 풋고추 건물중은 $348.4kg10^{-1}$으로 관행 경운 토양에 비하여 16% 증가되었으나, 무경운 3년차는 감소되었다. 무경운 2년차 토양의 세균은 관행 경운 토양에 비하여 유의적인 증가를 보였으나, 무경운 3년차에서는 관행 경운 토양과 유의적인 차이가 없었다. 방선균도 경운 토양에 비하여 무경운 2년차 토양은 유의적인 증가를 보였으나 무경운 3년차는 감소되었다. 곰팡이는 경운토양에 비하여 무경운 2~3년차 토양에서 1.3~1.7배 정도 증가되었다. 무경운 재배 토양의 $CO_2$ 발생량은 경운재배 토양에 비하여 41% 수준으로 발생량이 현저하게 감소되었다. 고추 생육 초기 관행 경운 토양의 미소동물은 톡토기목과 응애목 2종 6개체가 포획되었으나, 무경운 1년차 토양에서는 지네강 등 5종 11개체가 포획되었고, 무경운 2년차는 딱정벌레와 지네강 등 3종 5개체가 포획되었다. 고추 정식 46일 후에 조사한 경운재배 토양은 보라톡토기 등 4종 40개체를 포함 큰집게벌레 등 8종 97개체가 포획되었다. 무경운 재배토양은 9~10종 101~107개체가 포획되었다. 지표생물로서 환경의 변화의 기준이 되는 자연도 점수는 관행경운양 19점에 비하여 무경운 토양의 자연도 점수는 33점으로 1.74배 정도 높았다. 따라서 두둑과 고랑을 재활용한 한국형 무경운 농업은 관행 경운토양에 비하여 작물의 생육과 토양 미생물, 토양 곤충을 포함한 토양생물 다양성, 온실가스 발생량 감소에 긍정적인 역할을 하는 것으로 판단되었다. Hot pepper growth in no-tillage cultivation on recycled ridge was increased by 22% compared with tillage cultivation. At 3 years after no-tillage cultivation, hot pepper growth was increased by 12% compared with tillage cultivation. Dry weight of unripe hot pepper at 2 years of no-tillage cultivation was 348.4 kg/10a increasing 16% compared with tillage cultivation while dry weight of unripe hot pepper was decreased at 3 years of no-tillage cultivation. Bacteria flora at 2 years of no-tillage cultivation was significantly increased compared with tillage cultivation. Bacteria flora was not significantly different at 3 years of no-tillage cultivation. Actinomyces flora at 2 years of no-tillage cultivation was significantly increased compared with tillage cultivation. Actinomyces flora was decreased at 3 years of no-tillage cultivation. Fungi flora at 2 and 3 years of no-tillage cultivation was increased by 1.3 and 1.7 times respectively, compared with tillage cultivation. Generation amount of carbon dioxide at no-tillage cultivation soil was remarkably decreased by 41% compared with tillage cultivation. Population of animalcule in early stage of hot pepper soil was 2 species and 6 individuals on Collembola and Acari at tillage cultivation. Population of animalcule in hot pepper soil was 5 species and 11 individuals including Chilopode at one year of no-tillage cultivation. Population of animalcule in hot pepper soil was 3 species and 5 individuals including Coleoptera and Chilopode at 2 years of no-tillage cultivation. Population of animalcule was 4 species and 40 individuals including Hypogastrurigae and 8 species and 97 individuals including Earwig (Labidura japornica) at 46 days after transplanting on tillage cultivation. Population of animalcule was 9~10 species and 101~107 individuals on no-tillage cultivation. Nature status for environmental change as index organism was 19 points and 33 points, at tillage and no-tillage cultivation, respectively. These results indicate that no-tillage agriculture of korean-style on recycled ridge plays a very important roles on pepper growth, biodiversity of animalcule, and greenhouse gases at plastic film greenhouse soil in no-tillage systems.

Field Evaluation of Bacillus velezensis CE 100 on Growth Improvement of Black Raspberry (Rubus coreanus)

Kwan Woo Lee(이관우),Yeon Soo Han(한연수) 한국토양비료학회 2022 한국토양비료학회 학술발표회 초록집 Vol.2022 No.10

두둑을 재활용한 한국형 무경운 유기 농업 V. 분할 관수와 유기물 처리에 의한 시설 무경운 유기재배 고추의 수량 및 토양 화학성과 미소 곤충의 변화

양승구,김도익,김희권,양정고,한연수,정우진,Yang, Seung-Koo,Kim, Do-Ik,Kim, Hee-Kwon,Yang, Jung-Ko,Han, Yeon-Soo,Jung, Woo-Jin 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.2

본 논문은 한국형 무경운 재배 토양에서 유기물 투입과 분할 관수가 고추 생육 및 수량과 토양 화학성, 생물다양성에 미치는 영향을 구명하고자 추진하였다. 시험 후 토양에 화학성을 조사한 결과 토양 pH는 5.6~6.2, 유기물 함량은 $32{\sim}42g\;kg^{-1}$, EC는 $1.0{\sim}2.7dS\;m^{-1}$, 치환성 K은 $0.08{\sim}0.24cmol^+kg^{-1}$, Ca은 $9.5{\sim}12.8cmol^+kg^{-1}$, Mg은 $2.7{\sim}3.2cmol^+kg^{-1}$ 수준이었으며, 유효인산은 $1,011{\sim}1,137mg\;kg^{-1}$ 수준이었다. 무처리에서 토양에 미소동물은 톡토기와 응애류 등 5종 54개체가 포획되었으나, 대두박을 투입한 처리는 9종 271개체가 포획되어 되었다. 토양환경의 지표가 되는 자연도 점수가 대두박 투입 처리는 11점으로 무 투입 5점에 비하여 2배 정도 증가되었다. 대두박 투입은 무 투입에 비하여 고추의 수확과수의 감소로 수량이 감소되는 경향이었다. 그러나 대두박 무 투입 조건에서 표준시비량의 33~66% 수준에서 고추 수량은 증수되었으며 대두박 투입에서는 표준시비량의 33% 수준에서 증수되었다. 2회 분할관수는 1회 전량관수에 비하여 고추의 수확 과수가 12.5~34.9% 증가되었고, 수량은 13.5~34.4% 정도 증수되었다. This study was carried out to investigate the hot pepper yield, chemical properties of soil and distribution of animalcule with split irrigation and input of organic matter under no-tillage hot pepper green house condition. After experiment, soil pH maintained range of 5.6~6.2 in whole treatments. Organic matter content of soil was range of $32{\sim}42g{\cdot}kg^{-1}$. Soil salinity (EC) content of soil was range of $1.0{\sim}2.7dS{\cdot}m^{-1}$. Exchangeable cations in soil were range of $0.08{\sim}0.24cmol^+{\cdot}kg^{-1}$ in K, $9.5{\sim}12.8cmol^+{\cdot}kg^{-1}$ in Ca, and $2.7{\sim}3.2cmol^+{\cdot}kg^{-1}$ in Mg. Avaliable phosphorus ($P_2O_4$) content in soil was range of $1,011{\sim}1,137mg{\cdot}kg^{-1}$. Yield of pepper was more decreased in treatment of soybean cake fertilizer than no-treatment of soybean cake fertilizer. Yield of pepper in treatment of soybean cake fertilizer was increased at 33% of standard fertilizer application. Yield of pepper in no-treatment of soybean cake fertilizer was increased at 33% and 66% of standard fertilizer application. Number of fruits was increased range of 12.5~34.9% at half division irrigation compared with whole quantity irrigation. Yield of pepper was increased range of 13.5~34.4% at half division irrigation compared with whole quantity irrigation. Nine index of nature status and 271 Individual were captured in treatment of soybean cake fertilizer. Five index of nature status and 54 Individual were captured in treatment of soybean cake fertilizer. Nature status for environmental change as index organism was 11 points and 5 points, at treatment of soybean cake fertilizer and no-treatment of soybean cake fertilizer, respectively.

CO-I 유전자 기반 국내 유통 Corbicula 속 패류의 종 동정

박소영,강세원,황희주,정종민,송대권,박홍석,한연수,이준상,강정하,이용석,Park, So Young,Kang, Se Won,Hwang, Hee Ju,Chung, Jong Min,Song, Dae Kwon,Park, Hong Seog,Han, Yeon Soo,Lee, Jun-Sang,Kang, Jung-Ha,Lee, Yong Seok 한국패류학회 2016 The Korean Journal of Malacology Vol.32 No.2

The natives of the genus Corbicula have shown worldwide dispersion in recent times, which has caused great ecological and economic impacts on the introduced ecosystems. The species reported from the genus have been consumed as food and explored for medicine with pharmacological activity. Consequently, the demand of Corbicula sp. in the South Korean domestic market has increased and so also it's associated import to the country. However, due to the absence of identification keys of imported Corbicula, the market is facing confronting situations. We hypothesized that the mitochondrial Cytochrome Oxidase I gene (CO-I) based molecular profiling could be a necessary technique for identification of Corbicula sp. in the South Korea domestic market. The genetic analysis identified both Corbicula japonica and Corbicula fluminea from the market foods. C. japonica and C. fluminea are inhabitants in Korea, but C. fluminea production has decreased in Seomjingang river basin. Therefore, C. fluminea identified from this study, is expected to be imported from China and would have a mixed sales in Seomjingang river side basin. CO-I서열의 생물정보학적 분석 결과 국내에서 유통되는 재첩류의 연체부는 일본재첩 (C. japonica) 과 재첩 (C. fluminea) 으로 동정되어 두 종이 혼재하여 유통되는 것으로 확인되었다. 일본재첩 (C. japonica) 과 재첩 (C. fluminea) 은 국내에서 서식하고 있으나, 섬진강 유역의 재첩 (C. fluminea) 은 개체수가 감소하여 거의 생산되지 않고 있으므로, 본 연구를 통해 확인된 재첩 (C. fluminea) 은 중국 수입산으로 예상된다.

동양달팽이 (Nesiohelix samarangae) 의 expressed sequence tags (ESTs) 로부터 분리한 2종류의 Serpin 유전자 분석

박소영,정지은,황희주,왕태훈,박은비,김용민,이준상,한연수,양승하,이용석,Park, So Young,Jeong, Ji Eun,Hwang, Hee Ju,Wang, Tae Hun,Park, Eun Bi,Kim, Yong Min,Lee, Jun-Sang,Han, Yeon Soo,Yang, Seung-Ha,Lee, Yong Seok 한국패류학회 2014 The Korean Journal of Malacology Vol.30 No.2

동양달팽이의 EST 에서 serpin 유전자는 2가지 type이 추정되었다. Ns-serpin type 1 (partial) 서열의 코딩 영역은 총 819 bp 이었으며, 272개의 아미노산으로 이루어져 있었으며, Ns-serpin type 2 (partial) 의 코딩영역은 총 555 bp, 185개의 아미노산으로 이루어져 있었다. Laminarin 처리 전에는 Ns-serpin type 1이 2개 발현되었으며 처리 후에는 Ns-serpin type 2가 1개 발현되고 있었다. BLAST 결과를 바탕으로 유사도가 높은 17개의 참고 서열과 동양달팽이의 Ns-serpin type 1, 2의 아미노산 서열을 MEGA6 프로그램의 clustalW 엔진을 이용하여 multiple sequence alignment 를 수행한 결과, 척색동물의 포유강 9종, 조류강 1종, 양서강 1종과 척색동물인 해초강, 절지동물의 거미강 1종과 곤충강 1종이 같은 군으로 묶였으며, 연체동물문의 경우 동양달팽이의 두 가지 type 을 포함한 복족강 4종이 같은 군으로 묶이는 것을 확인하였다. Psipred 프로그램을 활용하여 예측한 2D 구조도 multiple align 및 phylodendrogram 결과와 연관이 있음을 확인할 수 있었다. EST를 통해 밝힌 동양달팽이의 두 가지 type의 Ns-serpin 서열은 Aplysia californica 등의 근연종들의 서열과 유사하였다. 본 연구에서는 무척추동물에서의 선천성 면역과 연관이 있는 것으로 잘 알려진 두 종류의 serpin 유전자 서열을 동양달팽이 EST 결과에서 발굴 하였으며, 동양달팽이에서도 여러 가지 type의 serpin 이 존재 할 수 있음을 입증하였다. Serpins are a group of proteins involved in the regulation of serine and other type of proteases, and have been identified in many kinds of organisms from invertebrates to vertebrates. Serpins are known to regulate the proteolytic cascades of the innate immune pathways in addition to their roles in blood coagulation, angiogenesis, fibrinolysis, inflammation and tumor suppression. In this study, we have isolated two partial serpin gene fragments from expressed sequence tags (ESTs) of Nesiohelix samarangae. Dotplot analysis indicates that they are of two different types, Ns-serpin type 1 and Ns-serpin type 2. Ns-serpin type 1 has 819 bp coding region (272 amino acids), whereas Ns-serpin type 2 has 555 bp coding region (185 amino acids). Molecular phylogenetic analysis shows that the identified serpins have high similarities to their counterparts in the California see slug, Aplysia californica. Yet, the precise biological and immunological roles of these Ns-serpins remain to be further investigated using RNA interference and other molecular techniques.

Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량

이효정(Hyo-Jeong Lee),박기범(Ki Beom Park),한연수(Yeon Soo Han),정래동(Rae-Dong Jeong) 한국식물병리학회 2021 식물병연구 Vol.27 No.3

식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작 물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협 이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예 작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되 고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라 서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital polymerase chain reaction (RT- ddPCR)을 확립하였다. TSWV 검출에 대한 RT-qPCR 및 RT-ddPCR의 민감도를 비교하였고, TSWV에 대한 RT-ddPCR의 특 이성 분석은 고추에서 주로 발생하는 바이러스 및 음성 대조군 에서 특이성을 확인한 결과 증폭되지 않았다. RT-ddPCR 및 RT- qPCR에 의해 측정된 TSWV의 선형회귀곡선은 모두 높은 선형 성을 나타냈지만, RT-ddPCR 분석이 10배 이상 더 민감하고 더 낮은 TSWV의 copy 수를 검출할 수 있었다. 종합적으로, 우리의 연구 결과는 RT-ddPCR이 TSWV 검출에 대해 높은 민감도와 특 이성을 제공하고 낮은 농도의 현장 시료에서 TSWV 검출하는 데 적합하다는 것을 보여준다. Plant viruses cause significant yield losses, continuously compromising crop production and thus represent- ing a serious threat to global food security. Tomato spotted wilt virus (TSWV) is the most harmful plant virus that mainly infects horticultural crops and has a wide host range. Reverse-transcription quantitative real-time PCR (RT-qPCR) has been widely used for detecting TSWV with high sensitivity, but its application is limited ow- ing to the lack of standardization. Therefore, in this study, a sensitive and accurate reverse transcription drop- let digital polymerase chain reaction (RT-ddPCR) method was established for TSWV detection. Additionally, we compared the sensitivities of RT-qPCR and RT-ddPCR for TSWV detection. Specificity analysis of RT-ddPCR for TSWV showed no amplification for main pepper viruses and negative control. TSWV transcripts levels measured by RT-ddPCR and RT-qPCR showed a high degree of linearity; however, the former yielded results that were at least 10-fold more sensitive and detected lower TSWV copy numbers than the latter. Collectively, our findings show that RT-ddPCR provides improved analytical sensitivity and specificity for TSWV detection, making it suitable for identifying low TSWV concentrations in field samples.

쌍별귀뚜라미(Gryllus bimaculatus) 산란방법 및 알 저장조건

김성연,김도익,구희연,김정은,김현진,이유범,김지수,김호혁,한연수,김영철,Kim, Seong-Yeon,Kim, Do-Ik,Koo, Hui-Yeon,Kim, Jung-Eun,Kim, Hyun-Jin,Lee, Yu-Beom,Kim, Ji-Soo,Kim, Ho-Hyuk,Han, Yeon-Soo,Kim, Young-Cheol 한국응용곤충학회 2020 한국응용곤충학회지 Vol.59 No.2

2016년에 쌍별귀뚜라미는 식품의약품안전처 식품공전에 등록된 식품원료로 가장 큰 장점은 월동 없이 연중 대량 사육이 가능하다는 것과 60% 이상의 단백질 함량이다. 본 연구에서는 사육공간, 인건비 등의 문제로 인하여 귀뚜라미 사육이 불가능할 때를 대비하여 저장조건을 설정하였으며, 산란율이 높은 산란처 선발과 알의 동종포식률을 조사하였다. 산란은 상토 산란처에서 가장 선호도가 높았으며(p < 0.05), 알의 동종포식률을 보았을 때 10 L 당 종령 약충 62마리 이하, 1일 동안 산란을 받는 것이 가장 적합한 것으로 판단되었다(p < 0.05). 알의 저온 저장을 하였을 때는 28℃에서 10일간 보관 후 16℃에서 10일간 저온 저장했을 때 부화율이 가장 높았고, 저장을 하지 않았을 때와 비교하였을 때 12~14일 부화를 연장할 수 있었다(p < 0.05). 쌍별귀뚜라미의 산란처, 산란 방법 및 알 저장조건을 설정함으로써 효율적인 생산방법을 제시하였다. In 2016, the two-spotted cricket was approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. Additionally, the two-spotted crickets have high protein content (60%) and can be reared throughout the year even during the overwintering period. In this study, storage conditions were set in case cricket breeding was not possible due to problems such as breeding space and labor costs, and selection oviposition mats to determine high fecundity rates and low cannibalism rates for the eggs were investigated. The oviposition mat was mostly composed of soil (p < 0.05). Less than 62 crickets per 10 litters were found to be best suited for 1 day of spawning, based on the cannibalism rate of the eggs during the egg laying period. The results from the hatched eggs indicated that the hatching extended 12 to 14 days, and the best hatching rate was approximately 85% when stored at low temperatures(16℃) for 10 days. An efficient production proposed method that established the best oviposition mat and egg storage method for the cricket.

식용 갈색거저리 유충에서 분리한 키틴으로부터 Serratia marcescens PRNK-1에 의한 N-아세틸글루코사민의 생산

서동준 ( Dong-jun Seo ),문채영 ( Chaeyeong Moon ),송용수 ( Yong-su Song ),최승희 ( Seung-hee Choi ),한연수 ( Yeon Soo Han ),조용훈 ( Yong Hun Jo ),노미영 ( Mi Young Noh ),정우진 ( Woo-jin Jung ) 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.3

S. marcescens PRNK-1로부터 얻어진 키틴분해 조효소와 식용 갈색거저리 유충으로부터 얻어진 콜로이달 키틴의 산업적 활용에 대하여 연구하였다. 식용 갈색거저리로부터 얻어진 키틴의 형태는 게 껍질에서 확인된 α-form 형태임을 X-ray 회절분석법과 FT-IR 분석법을 통하여 확인하였다. 게 껍질과 식용갈색거저리를 이용하여 제조한 콜로이달 키틴을 기질로 하여 S. marcescens PRNK-1로부터 얻어진 키틴분해 조효소와 반응한 결과 식용 갈색거저리 키틴에서 더 높은 함량의 N-아세틸글루코사민을 생성함을 확인하였다. 식용 갈색거저리 유충으로부터 얻어진 콜로이달 키틴이 S. marcescens PRNK-1로부터 얻어진 키틴분해 조효소와 반응하여 N-아세틸글루코사민 생산을 확인함으로써 산업적으로 응용 가능성이 매우 높을 것으로 기대된다. In this study, crab shell chitin and Tenebrio molitor larva (Mealworm) chitin were characterized by X-ray diffraction and FTIR analysis. From the X-ray diffraction results, α-form chitin was observed four crystalline reflections, shown at 9.4°, 19.3°, 20.8°, and 23.3° in crab shell chitin, and at 9.44°, 19.3°, 20.7°, and 23.3° in mealworm chitin by crystalline structure. From the FT-IR spectra results, α-form chitin showed doublet at amide I band in both crab shell chitin and mealworm chitin. Manufacturing process of colloidal chitin from mealworm was conducted with acid, alkali, and decoloration and then adjusted at pH 6~7 with 70% ethanol. Degradation pattern of colloidal chitin from crab shell and mealworm was investigated after reaction of chitinase-producing bacterium Serratia marcescens PRNK-1 by TLC and HPLC. Production of N-acetyl-glucosamine showed rapidly at 3 hr after reaction with crab shell and mealworm colloidal chitin and crude enzyme of S. marcescens PRNK-1 on TLC plates. N-acetyl-glucosamine was produced by 5,041.3 ppm and 5,319.8 ppm at 3 days in crab shell colloidal chitin and in mealworm colloidal chitin, respectively, after reaction with crude enzyme of S. marcescens PRNK-1. Our results indicate that the colloidal chitin obtained from mealworm could be used as useful industrial resources at production of N-acetyl-glucosamine.

연구보문 : 환경독성 ; 석창포 추출물의 모기에 대한 기피활성

이승호 ( Seung Ho Lee ),정진관 ( Jin Kwan Jung ),박상현 ( Sang Hyun Park ),김문식 ( Mun Sik Kim ),한연수 ( Yeon Soo Han ),서정미 ( Jung Mi Seo ),김인선 ( In Seon Kim ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.1

BACKGROUND: Acorus gramineus is of medicinal plants that exhibit variable biological activities for human health and against insect pests. The extracts of A. gramineus was examined in an attempt to develop a natural repellent against human disease-mediating Culex pipiens. METHODS AND RESULTS: The roots of A. gramineus dried under dark conditions were homogenized and extracted with ethanol. The extracts were subjected to repellent activity assays against C. pipiens in a hand-made acrylamide box with three accessible rooms. Significantly low number of mosquitos was found in the room previously fumigated with the extracts at 50mg/L on the filter paper, exhibiting less than 20% of mosquitos tested. More than 50% of mosquitos tested was found in the room without the extracts, but less than 30% was found in the room that released mosquitos. GC/MS analysis detected β-asarone as a main component of the extracts. The commercial asarones (α and β) showed a repellent activity at 50mg/L on the filter paper similar to the extracts. CONCLUSION(S): A. gramineus has potential for use as a mosquito repellent since β-asarone, a main component of the plant, exhibited a strong repellent activity against C. pipiens.