http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
산란계 개량에 관한 연구 1 . 순계의 생산능력 비교시험
상병찬,강만석,김동곤,한성욱 ( B . C . Sang,M . S . Kang,D . K . Kim,S . W . Han ) 한국축산학회 1980 한국축산학회지 Vol.22 No.5
This experiment was carried out to test performance of the domentic purebred fowl. The purpose of this experiment was to compare the productive performance of the purebred fowl to obtained basic materials for laying performance improvement by means of cross breeding. In this study, 1, 200 chickens was used-that is 6 purebred strains White Leghorns(720 chickens), 1 purebred strain Rhode Island Reds (120 chickens), 1 purebred strain New Hampshires (120 chickens). The results obtained in this study are as follows; 1. The average Viability of brooding, rearing and laying period was best for Rhode Island Reds and E. Strain among White Leghorns. 2. The average body weight at 8 weeks, first egg production days, 500days of age in W. L. was 546.86g. 1431.37g, 1738.34g, respectively, but there was no significant difference among. purebred strains of W. L. 3. The average Sexual maturity of 50% egg production was 170.58 days in W. L., but R. I. R. and S. N. H. were 189.94 days, 182.42days, respectively. There was significant difference at 1 % level among Strains. 4. The average hen-housed egg production was 214.16 eggs in W. L., but R. I. R. and S. N. H. were 214.22 eggs, 196.74 eggs, respectively. There was significant difference at 1% level among Strains, but the highest hen-housed egg production (231.84 eggs) was E. Strain among strains. 5. The average egg weight was 54. 48g in W. L, but R. I. R. and .S. N. H. were 56.12g, 54.90g, respectively, The highest egg weight (57.69g) was Coronial Strain of W. L. among strains. There was significant difference at 1 % Level among strains. 6. The average feed requirement was 3.01 in W. L. but R. I. R. and S. N. H. were 3.23, 3.52, respectively the highest feed requirement (2.79) was Coronial Strain of W. L. among strains. There was significant difference at 1 % level among strains. 7. On the basis of the results of this study, the productive performance was best for C. D. E. strains among W. L. and R. I. R.
상병찬,한성욱,오봉국,정선부 ( B . C . Sang,S . W . Han,B . K . Ohh,S . B . Chung ) 한국축산학회 1983 한국축산학회지 Vol.25 No.5
This study was conducted to improve the interior egg quality with a total of 6,093 eggs, laid at 351 Single Comb White Leghorns and 326 Rhode Island Reds, produced at National Livestock Breeding Station from March 1, 1979 through July 30, 1980. The results obtained are summarized as follows; 1. The average albumen height of the eggs at first eggs, 300 and 500 days of age was 8.32, 7.51 and 6.74㎜ in the S.C.W. Leghorn and 8.04, 6.94 and 6.05㎜ in the R.L.Red, and the Haugh units at first egg, 300 and S00 days of age was 90.92, 79.74 and 76.25 in the S.C.W. Leghorn, and 88.54, 80.02 and 74.56 in the R.I. Red, also the egg shell thickness at first egg, 300 and 500 days of age was 0.383, (1.364 and 0.334㎜ in the S.C.W. Leghorn and 0.354, 0.332 and 0.327 in the R.I. Red. 2. The heritability estimates in the S.C.W. Leghorn and R.I. Red were 0.398∼0.847 and 0.306∼0.634 for albumen height, 0.162∼0.594 and 0.125∼0.485 for Haugh units, 0.185-0.339 and 0.176∼0.402 for egg shell thickness, respectively. 3. The genetic correlation coefficients between the interior egg qualities were as follows. In the S.C.W. Leghorn and R.I. Red, the coefficients between albumen height and Haugh units, 0.432∼0.845 arid 0.397∼0.974; between albumen height and egg shell thickness, 0.046∼0.226 and 0.068∼0.197; between Haugh units and egg shell thickness, 0.089∼0.364 and 0.165∼0.327 ; respectively.
육성기사료의 양적제한급여가 겸용종계의 생산성에 미치는 영향
상병찬,강만석,김동곤,한성욱 ( B . C . Sang,M . S . Kang,D . K . Kim,S . W . Han ) 한국축산학회 1980 한국축산학회지 Vol.22 No.5
The effect of feed restriction during the rearing Period on the reproductive Performance was studied using 400 birds of dual purpose breeder pullet (Rhode Island Reds). During the rearing period (7∼21 week of age) feed was affered 80%, 70%, 60% of feed consumption of full feed group, All groups were full feed during laying period after 21 week of age. The results abtained are summarized as follows ; 1. Viability was not significantly affected by the feed restriction in either the rearing period or laying period of the experiment. 2. Body weight of 60% restricted group at 22 weeks of age was 75% of control group. At 72 weeks of age it was 95% of that of full feed group and not yet recovered completely. 3. Feed consumption of restricted groups from 22 to 72 weeks of age was some what more than that of control group, but total feed consumption per hen of all age was significantly different according to the feed restriction. 4. The degree of sexual maturity as measured by age at 50% egg Production was delayed from 9 to 27 days compared to control group according to the restricted intensity during rearing period. 5. Hen-day egg production (22-72 weeks of age) was range from 67.23 to 68.79% among treatment. There was not significant difference among treatment. 6. Average hen-housed egg production over 53g per bird to the end of the experiment was 154.2 eggs for control and 159.7 eggs for 80%, 157.4 eggs for 70%, 166.9 eggs for 60% restricted group. There was significant difference at 5 % level among treatment. 7. Average egg weight was significantly increased in restricted groups relative to control. The difference between restricted groups and control was highly significant average egg weight. 8. In the economic analysis, feed cost for 1 hatchable egg (over 53g) was in the highest with the control group and was decreased as the degree of feed restricted intensity. On the basis of the results of this study, it was suggested that in dual purpose breeder the feed restriction should be fed 60% of control group during the rearing period (7-21 weeks of age) and the body weight at 22 weeks of age should be kept at the level of 75 of the control in other to get better performance during the laying period.
PCR 기법을 이용한 한우의 κ-casein 과 β-lactoglobulin 유전자의 다형성
원유석,상병찬,신형두,김진호,한성욱 한국축산학회 1997 한국축산학회지 Vol.39 No.5
This study was performed to find genetic polymorphisms of κ-casein and β-lactoglobulin loci in Hanwoo using the PCR-RFLP. Genomic DNA was prepared from the blood of 327 Hanwoo. Amplified products of 530bp and 262bp fragments were obtained from the amplification of K-casein and β-lactoglobulin loci by PCR. The K -casein AA genotype showed a 530bp fragment in amplified products of K -casein loci with the restricted enzyme digestion of Taq I. The κ-casein AB genotype showed 530bp, 3446p and 186bp fragments and BB genotype showed 3446p and 186bp fragments. The β-lactoglobulin AA genotype showed 153bp and 109bp fragments in amplified products of β-lactoglobulin loci with the restricted enzyme digestion of Hae III. The β-lactoglobulin AB genotype showed 153bp, 109bp, 79bp and 74bp fragments and BB genotype showed 109bp, 79bp and 74bp fragments. The κ-casein genotype of Hanwoo gave frequencies of 45.9%, 37.7% and 16.4% for AA, AB and BB types. The rc-casein A and B alleles were 0.647 and 0.353, respectively. The β-lactoglobulin genotype of Hanwoo gave frequencies of 6.4%, 28.8% and 64.8% for AA, AB and BB types. The β-lactoglobulin A and B alleles were 0.208 and 0.792, respectively.
PCR 기법을 이용한 한국재래산양의 α s2-casein 유전자의 특성
이상훈,상병찬,한성욱,김지애,서길웅 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4
This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from the analysis of genetic characteristics of α_(S2-) casein gene in Korean Native goat using PCR-RFLP. This study confirmed the amplified product of 1.3kb fragment obtained from the amplification of a α_(S2-)casein loci by PCR. The frequency of a α_(S2-)casein genotype in Korean Native goat was 100% for AA type and the frequencies of a α_(S2-)casein genotype in Saanen were 80 and 20% for AA and AB genotypes. The frequency of a α_(S2-)casein A allele was 1.000 in Korean Native goat, and the frequencies of a α_(S2-)casein A and B alleles were 0.900 and 0.100 in Saanen, respectively. The nucleotide sequence of a α_(S2-)casein gene of Korean Native goat was 99.0% homology with 12 nucleotide sequences difference of that of goat reported in GeneBank(AJ238965.1, AJ238967.1) and was 95.0% homology with 57 nucleotide sequences difference of that of Holstein reported in Gene Bank(M94327). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphisms and sequences for a α_(S2-)casein gene should be used as basic data for preservation and improvement of genetic resources in Korean Native goat breeding.
상병찬,이상훈,류승희,서길웅,한성욱,김선균 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2
재래산양의 유전자원 보존과 유전적 개량을 위하여 재래산양과 수입산양의 genomic DNA의 유전적 다형을 분석하기 위하여 수행되었으며 재래산양과 수입산양의 유전적 판별 분석은 RAPD기법을 이용하였으며 공시품종은 재래산양 30두, 재래산양 교잡종 10두, 수입산양 10두를 이용하였다 재래 산양육과 수입 산양육의 판별을 위한 시료는 재래 산양육 10두와 수입 산양육 10두를 이용하였다. 이들로부터 얻어진 결과를 요약하면 다음과 같다. 1. 재래산양, 수입산양 및 재래산양 교잡종으로부터 추출된 genomic DNA는 전기영동에 의해 약 23kb 크기의 DNA을 얻을 수 있었으며 UV spectrophotometer를 이용하여 흡광도 A 260과 A280의 비율로 측정한 결과, 그 비율이 175~210의 범위로 순도는 비교적 양호한 결과를 얻었다. 2. 순수 재래산양의 유전자원의 보존을 위한 재래산양의 유전자 감식여부를 탐색하기 위하여 약 110 여종의 random primer를 이용한 RAPD 기법에 의하여 재래산양, 수입산양 및 교잡종의 다형성을 분석한 결과 random primer OPO-19(5'-CAA ACG TCG G-3')를 이용하였을 때 재래산양에서만 396bp에서 band 가 나타났으며 수입산양과 교잡종에서는 vand가 나타났으며 수입산양과 교잡종에서는 band가 나타나지 않았다. 3. 또한, 재래 산양육과 수입 산양육의 유전적인 차이를 구명하기 위한 RAPD 기법에 의한 genomic DNA의 다형성 분석에 있어서도 random primer OPO-19(5'-CAA ACG TCG G-3')를 사용하였을 때 396bp에서 재래 산양육에서는 band가 나타났지만, 수입 산양육에서는 band가 나타나지 않았다. This study was carried out to analyze the genetic polymorphisms of genomic DNA of blood and meat for conservation of the genetic resources and genetic improvement of Korean Native goat. The genetic identification between Korean Native goat and imported goat was examined using RAPD(random amplified polymorphisms DNAs) analysis with 30 Korean Native goat, 10 hybrid, 10 imported goat. 10 Korean native goat meat and 10 imported goat meat. The results obtained from this study were summarized as follows. 1. Genomic DNA from Korean native goat, hybrid and imported goat could be obtained above about 23kb size using 0.5% agarose gel electrophoresis and the ratio of optical density at 260nm to that at 280nm was between 1.7 and 2.0 using UV spectrophtometer instrument. 2. In the results of the gene identification between Korean Native goat and hybrid, and imported goat using RAPD methods with random primer of 110 kinds, only Korean native goat showed a specific band at about 369bp using a random primer OPO-19 (5’-CAA ACG TCG G-3’), but imported goat and hybrid not showed. 3 Also, in the results of the gene identification between Korean Native goat meat and imported goat meat using RAPD methods with random primer, Korean native goat only showed a specific band at about 369bp using a random primer No 19(5’-CAA ACG TCG G-3’). but imported goat not showed.