흰쥐에서 내측 편도핵의 전기 자극에 의한 췌액 분비 증가 기전

윤신희,한상준,조양혁,Yoon, Shin-Hee,Hahn, Sang-June,Jo, Yang-Hyeok 대한생리학회 1989 대한생리학회지 Vol.23 No.2

This study was conducted to investigate whether an electrical stimulation of medial amygdaloid nucleus in rats increases pancreatic secretion. And an involvement of vagus nerve or plasma secretin in this process was also studied. In fasting rats anesthetized with urethane, a monopolar stainless steel electrode was stereotaxically inserted into the right medial amygdaloid nucleus. Pancreatic juice was collected for 20 minutes, during which physiological saline or 0.01 N HCI (0.18 ml/min) was perfused into the duodenum with or without bilateral subdiaphragmatic vagotomy. In the medial amygdaloid group, an electrical stimulation was continuously applied to the medial amygdaloid nucleus during the perfusion period. After collection of pancreatic juice, blood was drawn from the abdominal aorta for determination of the plasma secretin level. The results were as follows: 1) The electrical stimulaion of the medial amygdaloid nucleus did not influence the pancreatic secretion in response to intraduodenal saline perfusion. 2) The stimulation of the medial amygdaloid nucleus significantly increased the pancreatic secretory response (volume, bicarbonate output) to the intraduodenal 0.01 N HCI perfusion, and the increases were abolished by vagotomy. 3) The plasma secretin concentration after the intraduodenal 0.01 N HCI perfusion was higher than that after the saline perfusion. However, neither the electrical stimulation of the medial amygdaloid nucleus nor vagotomy affected the plasma secretin concentration during the intraduodenal perfusion with saline or 0.01 N HCI. It is, therefore, suggested that the medial amygdaloid nucleus facilitates the pancreatic secretion (volume, bicarbonate) elicited by intraduodenal HCI perfusion through the vagus nerve.

Effect of Damage to Medial Amygdaloid Nucleus on Pancreatic Exocrine Secretion Stimulated by Hydrochloric Acid in the Rat

김명석,윤신희,한상준,김미혜,Kim, Myung-Suk,Yoon, Shin-Hee,Hahn, Sang-June,Kim, Mie-Hye The Korean Physiological Society 1988 대한생리학회지 Vol.22 No.2

본 연구는 십이지장내 생리 식염수와 0.01 N HCl을 주입했을때 내측 편도핵의 손상이 췌액 분비와 혈장 secretin 농도에 미치는 영향을 구명하기 위하여 44마리의 수컷 흰쥐를 사용하였다. 그 중 21마리의 흰쥐는 뇌정위 고정장치에 의해 내측 편도핵에 삽입된 전극을 통해 양측성으로 내측 편도핵을 파괴한 내측 편도핵 손상군이고, 나머지 23마리는 편도핵의 손상없이 동일한 수술 조작만을 가한 수술 대조군이다. urethan 마취후에 십이지장 근위부에 위치한 관을 통해 십이지장 내강에 0.01 N HCl 또는 생리 식염수(0.9% NaCl)를 0.18ml/min 속도로 주입하면서 20분간 췌액을 채취하였다. 췌액 체취후 복대동맥에서 채혈하여 혈장 secretin농도를 측정하였다. 내측 편도핵 손상군에서 십이지장내 생리 식염수는 물론 0.01 N HCl 주입에 의한 췌액량은 수술 대조군의 것보다 유의하게 감소하였다. 내측 편도핵 손상군의 췌액내 단백질량은 생리 식염수 주입시 수술대조군의 것보다 유의하게 감소하였고, 0.01 N HCI 주입시에는 유의하지는 않지만 감소한 경향을 보였다. 그러나 기초상태 및 염산 자극에 의한 혈장 secretin 농도는 내측 편도핵의 손상에 의하여 아무런 영향을 받지 않았다. 따라서 내측 편도핵은 기초 상태 및 염산 자극에 의한 췌액의 분비에 촉진적 영향을 미치는 것으로 생각되나, secretin 분비기전이 이 촉진 영향에 관련되는 것으로는 보이지 않는다. This study was undertaken to investigate the effect of the medial amygdaloid nucleus on the pancreatic exocrine secretion and plasma secretin concentration in 44 male albino rats. Twenty-three rats in which the medial amygdaloid nucleus was damaged bilaterally by radio frequency a.c. through stereotaxically inserted electrodes (medical amygdaloid group, MA) and twenty-one rats which received the same operation without damage (operated control, OC), were prepared. Under urethan anesthesia, 0.01 N hydrochloric acid (HCl) or physiological saline (0.9% NaCl) was infused at a rate of 0.18 ml/min into the duodenum for 20 minutes. Pancreatic jucie was collected for the 20 min infusion period. After collection of pancreatic juice, blood was sampled from the abdominal aorta for the radioimmunoassay of plasma secretin concentration. In the MA group, the exocrine pancreatic secretory response to 0.01 N HCI as well as saline infusion was significantly inhibited compared with that in the OC group. The pancreatic protein output of the MA group significantly decreased after the saline infusion and tended to decrease after the 0.01 N HCI infusion, compared with that of the OC group. However, there was no significant difference in plasma secretin concentration between the two groups. Therefore it is strongly suggested that the rat medial amygdaloid nucleus has a facilitatory influence on both basal and acid-stimulated pancreatic exocrine secretion, but the releasing mechanism of secretin appears not to be involved in the influence.

자유전자레이저의 개발현황과 전망

이병철,정영욱,박성희,한상준,Lee, Byung-Cheol,Jeong, Young-Ug,Park, Seong-Hee,Hahn, Sang-June 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.5

자유전자레이저는 고에너지 전자빔의 운동에너지를 빛 에너지로 바꾸는 장치로서, 다른 어떤 종류의 광원으로도 제공할 수 없는 넓은 범위의 파장가변성과 우수한 분광특성을 갖고 있어, 기초연구에서부터 산업응용에 이르기까지 다양하게 활용될 수 있을 것으로 기대된다. 이 논문에서는 자유전자레이저의 원리와 특성, 요소기술을 소개하고, 최근 주목받고 있는 테라헤르츠파 자유전자레이저와 에너지 회수형 고출력 자유전자레이저에 대하여 자세하게 소개하였다. Free electron lasers (FELs) are promising sources of coherent radiation that can provide users with radiations having a wide-range frequency-tunability and good spectral characteristics for basic science and industrial applications. Especially in Terahertz or X-ray ranges of spectrum, FELs can generate much stronger radiations than conventional light sources. In this paper, we introduce the working principles and key technologies of FELs, the status and the prospects of FEL developments.

X선 사진의 판독 효율 향상을 위한 노출과 현상 기법에 관한 연구

장선필 ( Seon Phil Jang ),박영만 ( Young Man Park ),한상준 ( Sang June Hahn ) 한국사진학회 2011 AURA Vol.0 No.25

This study was conducted to improve the reading efficiency of X-ray Photographs by changing the development condition. To improve the reading efficiency, the characteristic curve was changed by varying the development conditions according to the condition of the image. The contrast was increased in the film with a low contrast, and decreased in the film with a high contrast. Using the normal X-ray filming method, the parts that are difficult to identify were emphasized. It was particularly effective for identifying minute cracks or internal structures. In addition, in the cases wherein the image cannot be represented in one film due to the difference in depth or thickness, the depth of the image was adjusted according to the unique range of the film to improve the reading efficiency of X-ray Photographs. This method can be applied to all X-ray filming subjects as well as cultural properties. Based on this technique, the image in the X-ray Photograph can be emphasized and the reading efficiency for the hard-to-detect parts can be increased. It seems that this would be very helpful for the X-ray Photograph analysis.

내측 편도핵의 위산분비 촉진작용에서 미주신경의 역할

김명석(Kim, Myung-Suk),조양혁(Jo, Yang-Hyeok),윤신희(Yoon, Shin-Hee),한상준(Hahn, Sang-June),이덕주(Rhie, Duck-Joo),김정진(Kim, Chung-Chin) 대한생리학회 1989 대한생리학회지 Vol.23 No.2

Sixty-seven conscious rats prepared with chronic gastric fistula were studied to examine the effect of vagotomy on gastric secretory responses to medial amygdaloid stimulation. Gastric acid output was significantly increased by electrical stimulation of the medial amygdaloid nucleus, and the increases in acid secretion were completely eliminated by vagotomy. However, in rats subjected to stimulation of the medial amygdaloid nucleus plus vagotomy, acid output was almost same as that in only vagotomized rats. And vagotomy itself decreased the acid secretion significantly. These results indicate that the influence of electrical stimulaton of the medial amygdaloid nucleus on acid secretion is carried largely via vagus nerves. And also, without electrical stimulation of medial amygdaloid nucleus, acid secretion is controlled by way of vagus in rats. Plasma gastrin concentrations were measured following stimulation of the medial amygdaloid nucleus. Plasma levels of gastrin were not significantly altered by stimulation of the medial amygdaloid nucleus with or without vagotomy. It is therefore inferred from the above results that the facilitatory influence of the medial amygdaloid nucleus on the gastric acid secretion is mediated chiefly via vagal activity and that gastrin is not responsible for the increase in acid secretion in this process.

흰쥐에서 내측 편도핵의 손상이 염산 자극에 의한 췌장 외분비에 비치는 영향

김명석(Kim, Myung-Suk),윤신희(Yoon, Shin-Hee),한상준(Hahn, Sang-June),김미혜(Kim, Mie-Hye) 대한생리학회 1988 대한생리학회지 Vol.22 No.2

본 연구는 십이지장내 생리 식염수와 0.01 N HCl을 주입했을때 내측 편도핵의 손상이 췌액 분비와 혈장 secretin 농도에 미치는 영향을 구명하기 위하여 44마리의 수컷 흰쥐를 사용하였다. 그 중 21마리의 흰쥐는 뇌정위 고정장치에 의해 내측 편도핵에 삽입된 전극을 통해 양측성으로 내측 편도핵을 파괴한 내측 편도핵 손상군이고, 나머지 23마리는 편도핵의 손상없이 동일한 수술 조작만을 가한 수술 대조군이다. urethan 마취후에 십이지장 근위부에 위치한 관을 통해 십이지장 내강에 0.01 N HCl 또는 생리 식염수(0.9% NaCl)를 0.18ml/min 속도로 주입하면서 20분간 췌액을 채취하였다. 췌액 체취후 복대동맥에서 채혈하여 혈장 secretin농도를 측정하였다. 내측 편도핵 손상군에서 십이지장내 생리 식염수는 물론 0.01 N HCl 주입에 의한 췌액량은 수술 대조군의 것보다 유의하게 감소하였다. 내측 편도핵 손상군의 췌액내 단백질량은 생리 식염수 주입시 수술대조군의 것보다 유의하게 감소하였고, 0.01 N HCI 주입시에는 유의하지는 않지만 감소한 경향을 보였다. 그러나 기초상태 및 염산 자극에 의한 혈장 secretin 농도는 내측 편도핵의 손상에 의하여 아무런 영향을 받지 않았다. 따라서 내측 편도핵은 기초 상태 및 염산 자극에 의한 췌액의 분비에 촉진적 영향을 미치는 것으로 생각되나, secretin 분비기전이 이 촉진 영향에 관련되는 것으로는 보이지 않는다. This study was undertaken to investigate the effect of the medial amygdaloid nucleus on the pancreatic exocrine secretion and plasma secretin concentration in 44 male albino rats. Twenty-three rats in which the medial amygdaloid nucleus was damaged bilaterally by radio frequency a.c. through stereotaxically inserted electrodes (medical amygdaloid group, MA) and twenty-one rats which received the same operation without damage (operated control, OC), were prepared. Under urethan anesthesia, 0.01 N hydrochloric acid (HCl) or physiological saline (0.9% NaCl) was infused at a rate of 0.18 ml/min into the duodenum for 20 minutes. Pancreatic jucie was collected for the 20 min infusion period. After collection of pancreatic juice, blood was sampled from the abdominal aorta for the radioimmunoassay of plasma secretin concentration. In the MA group, the exocrine pancreatic secretory response to 0.01 N HCI as well as saline infusion was significantly inhibited compared with that in the OC group. The pancreatic protein output of the MA group significantly decreased after the saline infusion and tended to decrease after the 0.01 N HCI infusion, compared with that of the OC group. However, there was no significant difference in plasma secretin concentration between the two groups. Therefore it is strongly suggested that the rat medial amygdaloid nucleus has a facilitatory influence on both basal and acid-stimulated pancreatic exocrine secretion, but the releasing mechanism of secretin appears not to be involved in the influence.

흰쥐에서 내측 편도체가 위산 분비와 혈장 Gastrin 농도에 미치는 영향

윤신희(Yoon, Shin-Hee),김정진(Kim, Chung-Chin),김명석(Kim, Myung-Suk),조양혁(Jo, Yang-Hyeok),한상준(Hahn, Sang-June),김미혜(Kim, Mie-Hye),최현(Choi, Hyun) 대한생리학회 1989 대한생리학회지 Vol.23 No.1

This study was undertaken to investigate the effect of medial amygdala on the gastric acid secretion and plasma gastrin concentration in the rats with chronic gastric fistula. After the medial nucleus of amygdala was damaged bilaterally by radiofrequency a. c. through stereotaxically inserted electrodes, the gastric juice was collected in the basal and histamine-stimulated states for 1 hour. The gastric juice was also collected while the medial nucleus of amygdala was stimulated with biphasic square wave in the both states. After the collection of the gastric juice, blood samples were drawn from the abdominal aorta for the radioimmunoassay of plasma gastrin. The results were as follows: 1) The damage of the medial amygdala significantly decreased the gastric juice volume and the acid output in the histamine-stimulated state. 2) The electrical stimulation of the medial amygdala significantly increased the gastric juice volume and the acid output in the histamine-stimulated state, and the acid output in the basal state. 3) The damage of the medial amygdala significantly decreased the plasma gastrin concentration but the electrical stimulation of the medial amygdala did not affect the plasma gastrin concentration. It is therefore suggested that the medial amygdala has a facilitatory influence on the histamine-stimulated gastric acid secretion in rats, and the influence may not be attributed to gastrin release.

자기증폭자발방사에서의 Coherent bunched beam효과

한상준 中央大學校 基礎科學硏究所 1995 基礎科學硏究所 論文集 Vol.9 No.-

A general formalism of Self-Amplified-Spontaneous-Emission(SASE) in the linear regime is developed by deriving the coupled Maxwell-Klimontovich equations for an arbitrary density profile and including the effects of the energy spread, diffraction, and the betatron oscillation. The temporal and the spectral intensity profiles of SASE depend linearly on the initial electron correlation function. The correlation function consists of two terms, a term giving rise to the usual spontaneous radiation and its amplification to SASE, and a term representing the coherent bunched beam effect. The latter term has been neglected so far in the treatments of SASE, but it could be significant when there is a variation in the electron density at a length scale comparable to the wavelength. By the extensive numerical calculation, the comparison with the recent SASE experiment at Stanford University will be made.

Dilatant System의 유동메카니즘 : Effect of Electroytes on the Viscosity of Starch-Water Suspenions Starch-Water Suspension의 점성에 미치는 전해질의 영향

崔相元,韓相準,金應冽 漢陽大學校 基礎科學硏究所 1985 基礎科學論文集 Vol.4 No.-

Photato starch-water suspension에 KCI, Bacl₂, AICI₃의 전해질을 첨가 했을때, 각 농도변화와 전해질의 valene에 따라 suspension의 점도가 어떻게 변화 하는지를 couette type antomatic rotation viscometer를 이용하여 관찰하였다. 전해질의 양이온 원자가가 클수록, 또한 농도가 증가 할수록 dilatancy가 감소하는 것으로 나타났다. Verwey-Overbeek theory에 의하면 repulsive potential(??)은 전해질 농도와 valance가 증가됨에 따라 증가하며, 이러한 ??의 증가는 입자간의 수소결합을 약화시켜 Scaffolding structure가 쉽게 깨어지게 하므로 계의 Viscosity가 감소하게됨을 알았다. Starch-Water suspension의 rheological property에 있어서, 입자간 수소결합이 큰 영향을 미치고 있는 것을 알수 있었다. The viscosity changes of potato starch-water suspension containing various electrolytes (with KCI, BaCl₂, and AlCl₃) concentration and valences were measured by using a Coutte type automatic rotational viscometer respectively. A system, which has higher valence and larger electrolyte concentration, has smaller dilatancy phenomenon observed. According to the Verwey-Overbeek Theory, the repulsive potential(??) increases with increasing electrolyte concentration and valence, and the increasing of ?? inhibits hydrogen bond between starch granules. These results indicate clearly that the scaffolding structure becomes more unstable and the viscosity of suspension becomes smaller. The rheological properties of starch-water suspension can be explained by hydrogen bond system.

TiO₂(Rutile)-H₂O 현탁용액의 유동성질에 미치는 농도 및 온도효과

이철우,한상준,김응렬 漢陽大學校 基礎科學硏究所 1990 基礎科學論文集 Vol.9 No.-

TiO₂- water 현탁계의 유동성질을 계의 농도와 온도의 변화에 따라 Couette 형회전 점도계를 이용하여 측정하였으며 Ree-Eyring flow equation으로부터 유도된 dilatant flow equation에 적용하여 고찰하였다. Rutile-water 현탁계는 30%wt 이상의 고농도에서는 thixotropy를 나타내고 25%wt 이하의 농도에서는 dilatancy를 나타내었으며 유동에 대한 활성화 에너지는 약 14Kcal/mol정도였다. 본연구에서는 유동성질간의 전이를 한 물질 내에서 구조의 형성과 파괴가 경쟁적으로 일어난다는 견지에서 고찰하였다. Rheological properties of Rutile-water suspension with various conditions of particle concentrations and temperatures. The rheologicl properties were measured using Couette type rotational viscometer. And we calculated flow parameters with applied to BKHR flow equation. Rutile-water suspension shows dilatancy at lower concentration relatively (at below 28%wt.) but at higher concentration(above 30%wt.) thixotropic flow was observed. And the activation energy for flow was about 14Kcal/mol. In this paper we studied this transition in the view point of competitive rate process between structure formation and deformation in the same material.