탁리소독음 함유 크림의 피부탄력 개선 효과

하헌용 ( Hun-yong Ha ) 대한미용학회(구 대한미용과학회) 2017 대한미용학회지 Vol.13 No.2

Dermal elasticity is closely related to generation of wrinkles. Especially, around face and eyes, wrinkles are particularly affected by skin elasticity. Taklisodokeum, a traditional oriental medicinal prescription, is typically used as a medicine for dermatological diseases. Recently, dermal damages are increasing due to environmental factors and pollution. Therefore, we investigated the dermal elasticity effect of a Taklisodokeum-containing cream. The dermal elasticity improvement was measured on eye side of face using a cutometer. Measurements were performed in triplicate before the product use (0 weeks) and after its use for 2 and 4 weeks. The cream increased the average dermal elasticity from 0.831 A.U. when the test was started to 0.853 A.U. (by 2.65%) after 2 weeks (p<0.005) and to 0.874 A.U. (by 5.17%) after 4 weeks (p<0.000). The above results indicate that Taklisodokeum cream has an obvious dermal elasticity improvement effect.

백선 추출물의 간세포 손상에 대한 연구

하헌용 ( Hun Yong Ha ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.5

Objectives : This study was carried out to evaluate whether the water extract from cause the cellular damage in HepG2 cell line. It was reported that Dictamnus dasycarpus Turcz(DDT) intake induce poisoning symptoms in human population. These symptoms was closely related to liver toxicity, however, mechanisms for liver toxicity caused by DDT have not been elucidated exactly. Here, hepatotoxicity caused by DDT was evaluated using HepG2 cell line. Methods : Water extract of DDT was treated into HepG2 cell with various doses such as 0, 0.1, 0.5, 1.0 and 5.0 ㎎/㎖. In order to cell viability, both MTT and LDH assay were carried out. Also, apoptosis array kit was used to identify whether cell death caused by DDT is due to apoptosis or not. In addition, reactive oxygen species (ROS) was measured after treatment of water extract. Results : We found out significant changes in the apoptosis related factors of hepatocyte. The cell viability of HepG2 treated with DDT water extract was decreased in dose-dependent. Also most of the apoptosis related factors were significantly increased. We found out that Caspase 3, Cytochrome C and ROS had increased in dose-dependent. In addition, other apoptosis related factors Bcl 2 and Bax, which were also constant changes. However, there was no significance. Conclusions : These results suggest that water soluble extract of DDT is expected to have oral toxicity, including hepatocellular damage Therefore, it is suggested that DDT could cause various side effects and toxicity of clinical conditions.

죽력(竹瀝)의 인체 피부에 대한 안전성과 보습 효과

하헌용 ( Hun Yong Ha ) 대한미용학회(구 대한미용과학회) 2021 대한미용학회지 Vol.17 No.2

Bamboo is a useful plant resource for mankind. Bambusae Caulis in Liquamen (BCL) is one of the processed products of bamboo, and is made by refining the sap of bamboo through heating. Although various uses of bamboo have been known, clinical study of moisturizing effect has never been conducted. In the present study, the clinical trial was conducted in accordance with the clinical trial regulations of the KFDA. Skin safety test results were determined to be non-stimulation without any irritation. Clinical trials were conducted on a non-treated group, control group, and test group, and significant results were obtained. The amount of moisture present on the skin was measured using corneometer. The measurement time was at 0 h, 0.5 h, 3 h, 6 h. The moisturizing effect significantly increased by 155% at 0.5 h compared to the non-treated group (p<0.001). Meanwhile, at 6 h, the gap between the two test groups reduced. This study is characterized by the clinical study using raw materials without making formulations. The test results were also confirmed as dose-dependent results. Based on the results of this clinical study, it is hoped that the raw materials can be used in various cosmetic formulations.

냉증에 대한 산소챔버의 임상 효능 및 안전성 연구

하헌용 ( Hun Yong Ha ),윤달환 ( Dal Hwan Yoon ),고호연 ( Ho Yeon Go ),한용대 ( Yong Dae Han ),김남식 ( Nam Sik Kim ),남은영 ( Eun Young Nam ),김형준 ( Hyung Jun Kim ) 대한한방부인과학회 2013 大韓韓方婦人科學會誌 Vol.26 No.4

Cold hypersensitivity is regarded to be associated with blood circulation. This study is aims to evaluate the effects and safety of oxygen chamber therapy on cold hypersensitivity by comparing the temperature and Visual Analogue Scale. Methods: 42 outpatients who visited ○○ University Oriental Hospital from July 11th, 2013 to August 28th, 2013 were analyzed. Patients were subjected to thermometer, and those with thermal difference greater than 0.3℃ between upper arm and palm and also with more than VAS 4 of cold hypersensitivity were diagnosed with cold hypersensitivity. 42 outpatients diagnosed with cold hypersensitivity are divided into two groups, one is the experimental group consisted of 21 patients and other was control group consisted of 21 patients. The experimental group had oxygen chamber therapy 10 times for 4 weeks. Thereafter the effects of oxygen chamber therapy on cold hypersensitivity was analyzed with t-text using SPSS for Windows version 21. Results: After the oxygen chamber therapy, experimental group had considerable improvement on cold hypersensitivity, in consequence of decreasing rate of thermal difference and VAS of cold hypersensitivity. Ear deafness and hand numbness were reported as an adverse effects in experimental group, but there was no serious adverse effects. Conclusions: This clinical trial showed oxygen chamber therapy could be effective and safe to reduce cold hypersensitivity.

Hyaluronic Acid Micro Fill Patch와 황금 (Scutellariae Radix) 에탄올 추출물 병용의 피부 보습 효과

한상국 ( Sang-kuk Han ),하헌용 ( Hun-yong Ha ) 대한미용학회(구 대한미용과학회) 2017 대한미용학회지 Vol.13 No.1

Recently, hyaluronic acid (HA) has attracted attention as a potential moisturizing material. Since HA is closely related to the skin`s moisture content, several methods for increasing HA in the human skin have been described. HA micro fill patch is a kind of micro needle patch and is one method for physically injecting HA from outside of the human body. We designed an HA micro fill patch using sodium hyaluronate (200,000 Da), HA (5,000 Da) and xylitol. Separately, micro needle patches are often used with essences. We have produced a Scutellariae Radix ethanol extract (SREE)-containing essence to potentially impact HA synthesis. Next, we measured moisturizing effects of these treatments alone or in combination using a corneometer. After application of the HA micro fill patch alone and in combination with SREE, the average levels of moisture content in the areas of application increased significantly by 7.0% and 11.7%, respectively (p<0.05). These data indicate that co-administration of HA micro fill patch and SREE improve moisturizing effect in the human skin.

일당귀 에탄올 추출물의 Hyaluronic Acid 합성 효과에 대한 실험적 연구

강민서,하헌용,김희택,Kang, Min-Seo,Ha, Hun-Yong,Kim, Hee-Taek 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.1

Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and anti-aging. The purpose of this study is to examine the effects of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There was no cytotoxicity in $50{\mu}g/ml$ concentration Angelica acutiloba extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration Angelica acutiloba extract. Hyaluronic acid production was higher in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration Angelica acutiloba extract than control group. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin.

중학생들의 건강 관련 생활습관에 따른 BMI 연구

이창희,하헌용,김희택,김남송,Lee, Chang-Hee,Ha, Hun-Yong,Kim, Hee-Taek,Kim, Nam-Song 대한한방안이비인후피부과학회 2012 한방안이비인후피부과학회지 Vol.25 No.1

Objectives : The middle school students'life style were studied with focus on Body Mass Index(BMI). Information was collected during 1st June 2009 to 10th July from 397 students(both male and female) by the subjective survey centered on personal characteristic, understanding about health in general, everyday activities, eating habits, preference in food, understanding about diet and obesity, and thoughts about fitness and diet. Methods : Students were selected from six classes by convenience sampling, then the final analysis was performed on 397 self-administered questionnaires, excepting 5 questionnaires, which have insincere answers. The data collection was carried out by SPSS(V.14.0 English Version), and the data was verified by $x^2$-test, ANOVA, multiple regression analysis. Results and Conclusion : The survey subjects were in the following distribution of body weights - underweight: 63.6%, normal weight: 29.5% and 6.9% were overweight. There was significantly different between BMI and mothers' occupation and satisfaction with school life(p<0.05). As for the analysis(multiple regression analysis) on understanding about health, the factors that affect BMI are self recognition of figure and efforts to reduce weight. In terms of efforts for one's own health, 78.2% of under weight and 82.3% of normal weight were taking active initiatives. But 85.2% of the overweight students were not giving any efforts. As for the free time activities, more than half answered, 'playing with computers'(48.4 - 66.7%, 1.57 hours on average). As for TV, on average, 2.03 hours were spent in overall. And it turned out that the overweight students spent more time watching TV by 46.49 minutes(0.77 hours) on average(p<0.05). 7.4 hours was the average sleeping time. As for the eating habit,'quantity of meal, snack, meal between meals, fat reduction, and supplementary food'were shown to be related to the BMI(p<0.05). The instant food, meat and sweet food were shown to be also related to the BMI. In terms of knowledge about overweight and diet, only 8 students(2% of the total) answered all 10 questions correct. On average, 7-8 questions were correctly answered(53.9 %). When the scores were analyzed against the BMI by classifying the scores to high, middle and low categories, it showed a relationship by different weight group(p<0.02), and the overweight students had higher score by 0.82 compared to the underweight students. When thoughts and attitude on fitness and diet were analyzed in relationship to the BMI, the result was only - "I try to eat fruits more often"(fruits)(p<0.00).

초정광천수 도포가 피부장벽손상 회복에 미치는 효과

김용민 ( Yong-min Kim ),하헌용 ( Hun-yong Ha ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.2

Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease. Inflammatory responses in AD are related to transepidermal water loss (TEWL). This study examined the effect of Cho-Jeong mineral water (CJW) on TEWL. To induce skin barrier damage, epicutaneous patches with 1% sodium lauryl sulfate were applied to the volar forearm for 24 h. The level of TEWL in the experimental skin treated with CJW was lower than that in the control skin which was not treated with CJW. The average TEWL in skin treated with CJW decreased by 79.64% after 1 week of application and by 91.26% (p<0.05) after 2 weeks of application. These results suggest that CJW inhibits TEWL and may be a new alternative for the alleviation or prevention of AD.

왕호장근 뿌리 에탄올 추출물의 항산화 활성과 tyrosinase 활성 억제 효과

이동현 ( Dong Hyun Lee ),하헌용 ( Hun Yong Ha ),이평재 ( Pyeongjae Lee ) 대한미용학회 2016 대한미용학회지 Vol.12 No.1

In this study, quantitative analysis of emodin and polydatin in Fallopia sachalinensis rhizome harvested from the Ulleung island was performed using HPLC. In addition, the antioxidant activity and tyrosinase inhibitory effects were examined. The concentrations of emodin and polydatin in the ethanolic total extract (TE) were 3.3346 and 4.3314 μg/mg, respectively. Emodin was mainly detected in the hexane fraction (HX, 8.7281 μg/mg) and polydatin in ethyl acetate fraction (EA, 40.5763 μg/mg). Antioxidant activity was evaluated using the DPPH radical scavenging assay. DPPH scavenging activity was detected in TE, HX and EA in dose-dependent manners. Of them, EA displayed the highest levels of DPPH scavenging activity (SC50: 29.17 μg/mL). Using the tyrosinase inhibition assay, we determined that EA had the highest capacity to suppress tyrosinase activity. Taken together, these results suggest that F. sachalinensis rhizome has the potential to be developed for functional food and in the cosmetic industry. However, studies on F. sachalinensis-specific compounds and their precise biological effects will need to be further examined.

택사(Alismatis Rhizoma) 추출물의 급성 경구투여 독성 연구

석지현,노항식,정자영,하헌용,Seok, Ji-Hyun,Roh, Hang-Sik,Jeong, Ja-Young,Ha, Hun-Yong 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.4

Objectives : This study was carried out to evaluate the acute oral toxicity of Alismatis Rhizoma in Sprague-Dawley(SD) rats. Methods : male and female rats were administered orally with Alismatis Rhizoma water extract of 1,000 mg/kg (low dosage group), 2,000 mg/kg(middle dosage group) and 4,000 mg/kg(high dosage group). We daily observed number of deaths, clinical signs and gross findings for 7 days. After 7 days, we measured body and organs weight. Also we analyzed hematological changes. Results : No dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology and serum biochemistry. In addition no significant changes of gross body and individual organs weight. Conclusions : These results suggest that water soluble extract of Alismatis Rhizoma has not acute oral toxicity and oral $LD_{50}$ value was over 4,000 mg/kg in SD rats.