결핵성삼출성뇌막염(結核性渗出性腦膜炎)의 중서의결합치료(中西醫結合治療) (중의잡지 중심)(中醫雜誌 中心)

최해윤,김종대,Choi, Hae-Yun,Kim, Jong-Dae 대한한방내과학회 1998 大韓韓方內科學會誌 Vol.19 No.2

Pleural effusion means the inflammation of pleura which has a majority of respiratory disease. The main clinical manifestation is pleural effusional pain, dyspnea, cough, fever, etc. and at present the Tuberculous pleural effusion has the most frequency in which exists exudate in our country. And during studying oriental medical treatment about Tuberculous exudative pleural effusional patient, we found the clinical case about The Combination therapy of Chinese traditional and Western medicine at journal of traditional Chinese Medicine and considered it would be help in oriental medical treatment, so we adjust and report now. This study was performed by analyzing the six papers reported centering around the clinical case of The Combination therapy of Chinese traditional and Western medicine in journal of traditional Chinese Medicine published between 1990-1996. As these papers have no mistakes on diagnosis because it obtained pleurocentesis, tuberculin test positive reaction on choicing clinical case, definite results on X-ray, ultrasound as well as clinical basis, so it considers an apt conclusion. The results were as follows: 1. Western medical treatment uses chemical remedy same with pulmonary tuberculosis, and in case of tubercular pleuritis, it needs thoracic duct pyorrhea, and according to simple exudation also operates therapheutic pleural paracentesis. 2. In case of hydrothorax absorption about tuberculous pleural effusion, prescription of purge the heat accumulated in the lung and eliminate the retention of fluid with powerful purgatives shows considerable effects. 3. The latter period treatment of tuberculous pleural effusion needs Supplement qi and active the collaterals, Nourishing yin and clearing heat in addition to Supporting healthy energy to eliminate evils. 4. In case of curing tuberculous pleural effusion, The Combination therapy of Chinese traditional and Western medicine shows more considerable effect than single western medical treatment in absorption of hydrothorax. 5. In case of curing tuberculous pleural effusion, The Combination therapy of Chinese traditional and Western medicine shows more considerable effect than single western medical treatment in prevention of disease reappearance. 6. In case of curing tuberculous pleural effusion, The Combination therapy of Chinese traditional and Western medicine shows more considerable effect than single western medical treatment in vitality recovery at the latter period of disease.

백부근(百部根)에 관(關)한 문헌적(文獻的) 고찰(考察) (효능(效能) 및 약리작용(藥理作用)을 중심(中心)으로)

최해윤,김종대,Choi Hae-Yun,Kim Jong-Dae 대한한의학방제학회 1999 大韓韓醫學方劑學會誌 Vol.7 No.1

According to the study of literature on RADIX STEMONAE about its efficacy, pharmacological action, and clinical adaptive disease, the results are as follows; 1. About the efficacy of RADIX STEMONAE, it is known as moistening the lungs to arrest cough, and intestinal parasites from ancient to now, and dispelling phlegm is also known. 2. The clinical adaptation of RADIX STEMONAE is chronic bronchitis, pertussis, pulmonary tuberculosis in respiratory disease, and ascaricide for Ascaris, Enterobius vermicularis or eczema, pruritus, destroy louse for endermic liniment. 3. The pharmacological action of RADIX STEMONAE are antitussive, tuberculostatic, anthelmintic, antibacterial, antivirus, antifungal. 4. The antitussive mechanism of RADIX STEMONAE is central inhibitor for cough center of medulla oblongata, and the mechanisms of bronchial smooth muscle relax, and expectorant is also known.

가미음천이진탕(加味陰喘二陳湯) 투여환자(投與患者) 33례(例) 분석보고(分析報告)

최해윤,최영아,전연이,김종대,Choi Hae-Yun,Choi Young-Ah,Jeon Yeon-Yi,Kim Jong-Dae 대한한의학방제학회 2000 大韓韓醫學方劑學會誌 Vol.8 No.1

Objectives: We intented to find out the effect of Gami-Eumcheonyijintang to patients who complain with coughing and to make it useful for treating the patients with the symptom. Materials and Methods: We examined the medical of 33 patients who visited with the symptoms of coughing, sputum in Kyungsan University, Hospital of Oriental Medicine from April, 1996 to February, 1999. and we collected the result of the administration with Gami-Eumcheonyijintong. We aimed to find any change in the symptoms, reappearance after recovery, and any special facts with the medicine surveying by telephoning to the patients. Results : the results are as follows, 1. It showed that patients who were under thirties showed higher rate of recovery(75.00%) than who were over thirties(52.94% ). 2, The acute-stage patients who were recovered in less than four weeks showed higher rate of recovery than those chronic-stage patients who were recovered in more than eight weeks. 3. The patients who didn't have the symptoms of sputum or had less serious problems of it with severe coughing showed relatively the highest rate of recovery(73.91% ). 4. The patients who showed the coughing symptom right after URI without any past constitutional history showed the highest rate of recovery(73.68% ). Conclusion : From the above result, we found that Gami-Eumcheonyijintang is more effective in early coughing symptom after URI than chronic stage coughing symptom. It is thought that more studies about medicine composition are needed in the further.

Streptozotocin으로 유발된 당뇨백서에서 수종의 한약재가 혈당강하에 미치는 영향

최해윤 ( Hae Yun Choi ),정태영 ( Tae Young Jung ),서부일 ( Bu Il Seo ),김종대 ( Jong Dae Kim ),박동일 ( Dong Il Park ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2

N/A Objectives : This study was aimed to verify the hypoglycemic effects of the herbs which appear frequently as the effects of antipyresis & putting out the fire(淸熱○火)-Phellodendri Cortex(黃柏), Scrophulariae Radix(玄蔘), Radicis Lycii Cortex(地骨皮), Gardeniae Fructus(○子), Aloe(○○), Rehmanniae Radix(生地黃), Anemarrhenae Rhizoma(知母)-which were chosen among the herbs that were regarded as holding the hypoglycemic effects. Methods : To verify the hypoglycemic effects through the animal test, after the rats for test were treated 1 times with %mg/kg i.v. injection of streptozotodSigma, USA) which had been dissolved in the pH4.3 citrate buffer and were induced experimental hyperglycemia, the streptozotocin(ST2) induced hyperglycemic rats were prescribed with extract liquid 7.58 of each herb produced for test. And then the component such as glucose, insuline, and glucagon in serum and the change of body weight were measured and analyzed. Through that course, the significance for cure of DM were verified. Results : According to the booklet investigation, seven herbs which were useful for cure of DM were chosen and tested per 0s. As the result of that, It became evident that .Scrophulariae Radix, Anemarrhenae Rhizoma were significant for the hypoglycemic effects on DM. The insulin measurement showed the significant increase on both 3rd day and 7th day in the group of Scrophulariae Radix, Gardeniae Fructus, Aloe, Rehmanniae Radix and only showed the significant increase on 7th day ipl the group of Phellodendri Cortex, Radicis Lycii Cortex, Anemarrhenae Rhizoma. Besides, the glucagon measurement showed the significant decrease in the group of Radicis Lycii Cortex, Gardeniae Fructus, Rehmanniae Radix, Anemarrhenae Rhizoma. And the measurement of weight change showed the significant increase in all the sample herb groups. Conclusions : According to above result, Phellodendri Cortex, Scrophulariae Radix, Radicis Lycii Cortex, Gardeniae Frutus, Aloe, Rehmanniae Radix, Anemarrhenae Rhizoma were expected to be applied to the treatment of DM.

환경호르몬인 p,p-DDE의 흡입 시 ICR 마우스의 폐에 미치는 독성병리학적 연구

최해윤(Hae-Yun Choi),정태영(Tae-Young Jung),구세광(Sae-Kwang Ku),양희복(Hee-Bog Yang),이형식(Hyeung-Sik Lee) 한국독성학회 2005 Toxicological Research Vol.21 No.2

In order to monitor the histological and general profiles of lung after direct expose of p,p-DDE, 1, 5 and 10 ㎎/㎖ of p,p-DDE were sprayed to male ICR mouse, and seven days after exposure, changes of body weight, lung weight, clinical signs, histological profiles of lung and total WBC in blood were investigated with changes of total cell number and their differential count in bronchoalveolar lavage fluid (BALF). In the present study, a significant and dosage-dependent decrease of body weight was detected in p,p-DDE exposed groups and body weight gains during observational periods (7 days) were also significantly and dosage-dependently decreased in p,p-DDE exposed groups compared to that of vehicle control group. In addition general depression signs were detected in all p,p-DDE exposed groups with dosage-dependent manners, and lung weights were also increased in p,p-DDE exposed groups. Congestion, hemorrhage and severe exudate were observed in the lung of p,p-DDE exposed groups with sarcomatous changes and these signs were also showed by dosage-dependent manners. In addition, foreign body pneumonia signs were observed in the lung of p,p-DDE exposed groups in histological levels. The percentage of ALSA (Area of luminal surface of alveoli) was also significantly and dosage-dependently decreased in p,p-DDE exposed groups and total blood WBC and BALF cell numbers were significantly and dosage-dependently increased in p,p-DDE exposed groups compared to that of vehicle control group and increase percentage of neutrophil, eosinophil, and lymphocyte in BALF were monitored in p,p-DDE exposed groups compared to that of vehicle control group. In conclusion, severe allergic response and/or foreign body pneumonitic changes were induced by direct exposure of p,p-DDE containing beverage. So it is considered that strong and powerful regulation was need to control production of residence of environmental pollutant especially to p,p-DDE.

檳榔子 추출물의 마우스 경구 단회 투여독성 평가

최해윤(Hae Yun Choi) 한의병리학회 2013 동의생리병리학회지 Vol.27 No.3

인체폐암세포의 성장에 미치는 葦莖湯의 영향에 관한 연구

최해윤(Hae-Yun Choi),박철(Cheol Park),최영현(Yung Hyun Choi),박동일(Dong Il Park) 한의병리학회 2004 동의생리병리학회지 Vol.18 No.2

금은화가 LPS로 유발된 급성 폐 손상에 미치는 영향

이창건,최해윤,박미연,김종대,Yi, Chang-Geon,Choi, Hae-Yun,Park, Mee-Yeon,Kim, Jong-Dae 대한예방한의학회 2011 대한예방한의학회지 Vol.15 No.1

Objective : The object of this study was to observe the effects of Lonicerae Flos (LF) aqueous extracts on lipopolysaccharide (LPS)-induced rat acute lung injury. Method : Five different dosages of LF extracts were orally administered once a day for 28 days before LPS treatments, and then all rats were sacrificed after 5 hour-treatment of LPS. Eight groups of 16 rats each were used in the present study. The following parameters caused by LPS treatment were observed ; body weights, lung weights, pulmonary transcapillary albumin transit, arterial gas parameters (pH, $PaO_2$ and $PaCO_2$) bronchoalveolar lavage fluid (BALF) protein lactate dehydrogenase (LDH), and proinflammatory cytokines tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$) contents, total cell numbers, neutrophil and alveolar macrophage ratios, lung malondialdehyde (MDA), myeloperoxidase (MPO), proinflammatory cytokines TNF-${\alpha}$ and IL-$1{\beta}$ contents. In addition, the histopathologic changes were observed in the lung in terms of luminal surface of alveolus, thickness of alveolar septum, number of polymorphonuclear neutrophils. Result : As results of LPS-injection, dramatical increases in lung weights, pulmonary transcapillary albumin transit increases, increases in $PaCO_2$, decreases in pH of arterial blood and $PaO_2$, increases of BALF protein, LDH, TNF-${\alpha}$ and IL-$1{\beta}$ contents, total cells, neutrophil and alveolar macrophage ratios, TNF-${\alpha}$ and IL-$1{\beta}$ contents increases were detected with decreases in LSA and increases of alveolar septum and PMNs numbers, respectively as compared with intact control. These are means that acute lung injuries (resembling acute respiratory distress syndrome) are induced by treatment of LPS mediated by inflammatory responses, oxidative stress and related lipid peroxidation in the present study. However, these LPS-induced acute lung injuries were inhibited by 28 days continuous pretreatment of 250 and 500mg/kg of LF extracts. Because of lower three dosages of LF treated groups, 31.25 and 62.5 and 125mg/kg did not showed any favorable effects as compared with LPS control, the effective dosages of LF in LPS-induced acute lung injuries in the present study, is considered as about 125mg/kg. The effects of 250mg/kg of LF extracts showed almost similar effects with ${\alpha}$-lipoic acid 60mg/kg in preventing LPS-induced acute lung injuries. Conclusion : It seems that LF play a role in protecting the acute respiratory distress syndrome caused by LPS.

귀비탕(歸脾湯)이 비소세포 폐암세포(NCI-H520) 이식 마우스에서 항암 및 면역 활성에 미치는 영향

손지영,최해윤,김종대,Son, Ji-Young,Choi, Hae-Yun,Kim, Jong-Dae 대한한방내과학회 2012 大韓韓方內科學會誌 Vol.33 No.4

Objectives : This study was to observe anticancer and related immunomodulatory effects of Kwibi-tang extracts (KBTe) on non-small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice. Methods : Three different dosages of KBTe, 50, 100 and 200 mg/kg were orally administered once a day for 42 days from 11 days after tumor cell inoculation. Six groups, each of 8 mice per group were used in the present study. Changes in body weight, tumor volume and weight, lymphatic organs (spleen and popliteal lymph node), serum interferon (IFN)-${\gamma}$ levels, splenocytes NK cell activity and peritoneal macrophage activities, splenic tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-10 contents were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. The results were compared with a potent cytotoxic anticancer agent, 5-FU (5-Fluorouracil) 30 mg/kg, intraperitoneal treatment (3-day intervals for 42 days, the optimal effective treatment regimes already confirmed). Results & Conclusions : This study suggest that over 50 mg/kg of KBTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory effects. Although relatively lower anticancer effects were observed in KBTe 200 mg/kg treated mice as compared with 5-FU 30 mg/kg treated mice, no meaningful favorable immunomodulatory effects were observed after 5-FU treatment in the present study.

담훈(痰暈)환자의 임상적 고찰과 복부 적외선 체열촬영의 의의

박미연,최해윤,김종대,송광규,Park, Mee-Yeon,Choi, Hae-Yun,Kim, Jong-Dae,Song, Kwang-Kyu 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.2

To make a objective diagnosis of Dam Hun(痰暈), we investigated the 63 patients with Dam Hun(痰暈) and 34 normal people as control group. we compared the thermal difference between Chon-jung(CV17 ) and Chung-wan(CV12 中脘), Chon-jung(CV17 ) and Gwan-won(CV4 關元) and we compared the thermal difference of Yang-mun(ST21 梁門), Chun_chu(ST25 天樞), Su-do(ST28 水道), too. All 63 patients had dizziness and GI trouble. They had the symptom - fatigue, nausea vomitting, head heaviness, indigestion, cold limbs, headache, heart burn, constipation, diarrhea, anorexia. And some had edema, numbness, insomnia, palpitation, facial flushing, dysuria, ringing. Considering the average temparature of Chon-jung(CV17 ), Chung-wan(CV12 中脘), Gwan-won(CV4 關元), Yang-mun(ST21 梁門), Chun-chu(ST25 天樞), Su-do(ST28 水道) between the patients group and the control group, the patients group were lower than the control group except for Chon-jung(CV17 ), Gwan-won(CV4 關元), but the statistical significance was not. Considering the thermal difference of Chon-jung(CV17 ) and Chung-wan(CV12 中脘), Gwan-won(CV4 關元), Yang-mun(ST21 梁門), Chun-chu(ST25 天樞), Su-do(ST28 氷道) between the patients group and the control group, the temparature deviation of Chon-jung(CV17 ) and Chung-wan(CV12 中脘), Chon-jung(CV17 ) and Yang-mun(ST21 梁門) was significant. We suggest that coldness of upper abdomen is significant in diagnosis of Dam Hun(痰暈).