초고압처리가 생막걸리의 저장기간 연장에 미치는 영향

최한석,강창수,Choi, H.S.,Kang, C.S. 국립한국농수산대학교 교육개발센터 2020 현장농업연구지 = Journal of practical agricultural resear Vol.22 No.2

생막걸리의 유통기간은 연장하고자 UV조사와 초고압처리를 하였다. UV조사는 막걸리내 미생물을 감소시키지 못하였으나 초고압처리는 급격하게 감소시켰다. 막걸리의 생균수는 2.2-5.8 × 10⁷ CFU/mL이었으나 300MPa로 1분간 처리하였을 때 1.4-10 × 10³ CFU/mL로 감소하였고 400MPa 처리에 의해서는 4-68 CFU/mL, 500MPa 처리에 의해서는 40CFU/mL이하로 감소하였다. 효모는 400MPa 압력에서 사멸하였고 Bacillus amyloliquefaciens와 Rummeliibacillus stabekisii 500MPa 압력에서도 생존하였다. 400과 500MPa의 압력으로 처리한 막걸리는 상온(25℃)에서 30일간 저장하여도 균체수가 증가하지 않았고 알코올, 산도, 아미노산도의 변화도 억제되었다. 그러나 30일 이상 상온에서 저장하면 막걸리의 산도와 아미노산도가 증가하면서 음용이 어려웠다. 저온(4℃)저장에서는 저장 70일까지 막걸리의 품질변화를 억제시켜 신선한 막걸리와 유사한 맛을 가지고 있었다. To extend the shelf life of draft makgeolli, UV irradiation and high hydrostatic pressure were conducted. UV irradiation did not reduce the total cell number in makgeolli, but high hydrostatic pressure treated drastically decreased. The number of viable cells in makgeolli was 2.2-5.8 × 10⁷ CFU/mL, but when treated with 300 MPa pressure for 1 minute, it decreased to 1.4-10 × 10³ CFU/mL, with 400 MPa to 4-68 CFU/mL, and with 500 MPa to under 40 CFU/mL. Yeast died at 400 MPa pressure, but Bacillus amyloliquefaciens and Rummeliibacillus stabekisii survived at 500 MPa pressure. The makgeolli treated with 400 and 500 MPa pressure did not increase the number of cells even when stored at room temperature (25℃) for 30 days, and changes in alcohol, acidity, and amino acid vlaue were also suppressed. However, when stored for more than 30 days at room temperature, the acidity and amino acid levels of makgeolli increased, making it difficult to drink. At low temperature (4℃) storage, the quality change of makgeolli was suppressed until 70 days of storage, so it had a taste similar to that of fresh makgeolli.

이온交換樹指에 依한 토리움分離

최한석,하영구,Choi, Han-Suk,Ha, Young-Gu 대한화학회 1961 대한화학회지 Vol.5 No.1

Ion exchange procedure was studied for the separation of thorium from the acidic solution obtained by means of decomposition of monazite with alkali solution. Present cation exchange method consists of adsorption of cations from the sample solution (ca. 0.6N HCl acidic) onto Amberlite IR-120 resin, elution of all of the rare earth cations with 700 ml. of 2N Hydrochloric acid, and recovery of the thorium by elution with 200ml. of 6N sulfaric acid. Thorium recovery by the ion-exchange method mentioned above, was quantitative, and it is concluded that this ion-exchange method may be used not only for industrial separation of thorium from rare earths but also for quantitative determination of thorium with relative error, ${\pm}1.0.$.

稀土類中 토리움의 分離定量 에 관하여 (第一報) 過酸化水素法에 對한 檢討

최한석,박순자,Choi, Han-Suk,Park Soon Za 대한화학회 1957 대한화학회지 Vol.4 No.1

On the performance of the hydrogen peroxide method for Th-determination we encountered some difficulties, namely the formation of the insoluble matter occured in the nearly neutral solution after evaporation. We carried out the Th-determination in the mixture of the Th and some kind of the rare earth, and found that the insoluble matter was not formed in case of the mixture of Th and some kind of rare earth, but formed in case of Monazite. The formation of the insoluble matter in the case of Monazite sand, however, could be avoid by using the beaker instead of the evaporating dish and by adding the water repeatedly before it was completely evaporated to dryness.

재래누룩에서 분리한 N4와 N9 효모의 증류식 소주 양조특성

최한석,강지은,정석태,김찬우,백성열,여수환,Choi, Han-Seok,Kang, Ji-Eun,Jeong, Seok-Tae,Kim, Chan-Woo,Baek, Seong-Yeol,Yeo, Soo-Hwan 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.5

Soju is a Korean traditional distilled alcoholic beverage produced from mashes various crops and Nuruk which is cultured with wild microorganisms. This study was conducted to investigate rice-Soju brewing characteristics of yeasts isolated from Korean traditional Nuruk. The general components of rice (Hanarumbyeo) raw materials were 14.7 g of water, 6.8 g of crude protein, 0.9 g of crude lipid, 0.4 g of crude ash, and 76.5 g of carbohydrate in 100 g. Saccharifying and proteolytic activities in Hanarumbyeo ipguk (solid-state culture of Aspergillus luchuensis) were also determined. The alcohol content of the fermented wash from isolates was 15.37-16.58% (v/v), which is 16.7-36.0% higher than that of industrial yeasts (12.33-13.19%). Reducing sugar contents were 2.04-3.92 and 7.92-8.78 g/100 mL in the isolates and industrial yeasts, respectively. The isolated yeasts showed 25.2-52.7% higher yield of distillates (41% alcohol) compared to industrial yeasts. Forty-one components were detected in the rice distillated Soju (25% alcohol) and principal component analysis revealed differences between the isolated and industrial yeasts with respect to the contents of i-BuOH, isobutanal diethyl acetal, ethyl caprate, and tetradecanoic acid. 재래누룩에서 분리한 효모인 N4와 N9에 대해 쌀 증류식 소주제조 특성을 살펴보았다. 원료 쌀(한아름 품종)의 일반성분은 수분 14.7, 조단백질 6.8, 조지방 0.9, 조회분 0.4, 탄수화물 76.5 g/100 g이었다. 제조된 입국의 총산 함량은 2.92%(citric acid, dry base)였으며 효소활성에 있어 당화력은 926.72, ${\alpha}$-amylase 52.40, gluco-amylase 887.71, ${\alpha}$-glucosidase 0.14, acidic carboxypeptidase 17,335.73, ${\beta}$-glucosidase 174.46 U/g dry base로 나타났다. 술덧의 알코올 함량은 재래누룩 분리 효모가 15.37-16.58%로 상업용 효모 12.33-13.19%보다 16.7-36.0% 높은 것으로 나타났다. 반면, 환원당 함량은 각각 2.04-3.92, 7.92-8.78 g/100 mL로 상업용 효모의 당 이용률이 누룩분리 효모보다 낮았다. 이러한 결과로 증류 후 원주의 획득량이 누룩분리 효모에서 25.2-52.7% 높았다. 쌀 증류식 소주(알코올 25%)에서 41개의 휘발성 성분이 검출되었으며, 주성분 분석 결과 누룩분리 효모와 상업용 효모는 i-BuOH, isobutanal diethyl acetal, ethyl caprate, tetradecanoic acid 성분의 함량 차이가 있는 것으로 나타났다.

인공재배 버섯의 혈전분해효소 활성검색

최한석(Han-Seok Choi),김명곤(Myung-Kon Kim),박효숙(Hyo-Suk Park),김재성(Jae-Sung Kim),심명화(Ming-Hva Shen),김성준(Sung-Jun Kim) 한국식품과학회 2005 한국식품과학회지 Vol.37 No.6

누룩미생물을 이용한 잡곡발효음료의 제조

최한석(Han Seok Choi) 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

3D 콘텐츠 제어를 위한 키넥트 기반의 동작 인식 모델

최한석(Han Suk Choi) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.1

본 논문에서는 키넥트 적외선 프로젝터를 통해 깊이를 감지할 수 있는 카메라를 이용하여 사람 움직임을 추적하고 본 논문에서 제안한 몸동작 모델 인식을 통하여 3D 콘텐츠를 제어하는 기법을 제안 한다. 본 논문에서 제안하는 사람의 동작 인식 모델은 사람의 오른팔과 왼팔의 손목, 팔꿈치, 어께 움직임의 거리를 계산하여 좌, 우, 상, 하, 확대, 축소, 선택 등의 7가지 동작 상태를 인식한다. 본 연구에서 제안한 키넥트기반의 동작 인식 모델은 기존의 접촉식 방식의 인터페이스와 비교할 때 특정센서 또는 장비 부착에 대한 불편함을 없애고 고비용의 하드웨어 시스템을 이용하지 않음으로서 사람의 자연스런 몸동작 이동에 따른 저 비용 3D 콘텐츠 제어 기술을 보여준다. This paper proposes a kinect-based human motion recognition model for the 3D contents control after tracking the human body gesture through the camera in the infrared kinect project. The proposed human motion model in this paper computes the distance variation of the body movement from shoulder to right and left hand, wrist, arm, and elbow. The human motion model is classified into the movement directions such as the left movement, right movement, up, down, enlargement, downsizing. and selection. The proposed kinect-based human motion recognition model is very natural and low cost compared to other contact type gesture recognition technologies and device based gesture technologies with the expensive hardware system.

장수버섯 배양법에 의해 urushiol이 제거된 발효옻 추출물이 된장발효에 미치는 영향

최한석 ( Han Seok Choi ),정석태 ( Seok Tae Jeong ),최지호 ( Ji Ho Choi ),강지은 ( Ji Eun Kang ),김유진 ( Eu Gene Kim ),노종민 ( Jong Min Noh ),김명곤 ( Myung Kon Kim ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

된장제조공정에 발효옻 추출물을 첨가하고 염수발효기간 동안의 미생물, 효소활성, 유리아미노산, 유기산 및 일반성분 변화를 조사하였다. 염수발효 42일 경과 후 추출물 첨가군(0.7%, 2.0%, 5.0%, 10.0%, w/v)의 염수 중 총 유리아미노산 함량은 2188.3, 4634.7, 2982.7, 5070.6 mg/100mL로 대조군 보다 각각 1.3, 2.8, 1.8, 3.1배 높게 나타났다. 염수를 걸러낸 된장의 미생물 분포는 일반세균 0.3-12.0 × 108, 곰팡이 3.0-21.0 × 104, 효모 1.0-2.0 × 104, 대장균 불검출, B. cereus 3.0-25.0 × 102cfu/g으로 추출물 첨가에 의해서 일반세균과 곰팡이의 생균수가 1 log cycle 범위 내에서 변하였을 뿐 큰 변화는 관찰되지 않았다. 발효옻 추출물 첨가에 의해서 skim milk 분해활성은 13.8-26.0%, starch 분해활성은 16.1-35.1%, acidic protease의 활성은 1.8-2.5배, 1.9, a-amylase의 효소활성은 6.4-7.0배 증가되었다. 발효옻 추출물이 첨가된 된장의 총 유리아미 노산 함량은 각각 855.97, 1899.01, 1675.03, 1733.07 mg%로 추출물 첨가에 의해서 1.4-3.0배 상승하였으며, alanine, arginine, aspartic acid, citrulline, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, valine 등이 주요 유리 아미노산이었다. 유기산은 lactic, acetic, pyroglutamic acid가 주요 유기산이었다. 일반성분은 pH, 수분, 회분, 염도, 아미노산성 질소 함량이 증가하였다. The effect of fermented Rhus verniciflua stem bark (FRVSB) extract on the microbial count, enzyme activity, concentrations of free amino acids and organic acids, and physiochemical properties of doenjang (soybean paste) was evaluated during brine fermentation. The FRVSB extract increased the total free amino acid concentration by 1.3-3.1-fold on the 42nd day of brine fermentation. After the filtration of brine, the following microbial counts were obtained in the doenjang: bacteria, 0.3 × 108-12.0 × 108cfu/g; mold, 3.0 × 104-21.0 × 104cfu/g; yeast, 1.0 × 104-2.0 × 104cfu/g; Escherichia coli, not detected; and Bacillus cereus, 3.0 × 102-25.0 × 102cfu/g. The FRVSB extract addition enhanced the protein and starch degrading activity by 13.8-26.0% and 16.1-35.1%, respectively. The extract increased the total free amino acid content by 1.4-3.0-fold. Lactic acid, acetic acid, and pyroglutamic acid were the predominant organic acids in doenjang. Moreover, the proximate composition, pH, moisture, ash, salt, and amino nitrogen content were increased.

보문 : 장수버섯 균사체가 배양된 옻피의 이화학적 특성 및 생리활성

최한석 ( Han Seok Choi ),김보현 ( Bo Hyun Kim ),여수환 ( Soo Hwan Yeo ),정석태 ( Seok Tae Jeong ),최지호 ( Ji Ho Choi ),박효숙 ( Hyo Suk Park ),김명곤 ( Myung Kon Kim ) 한국균학회 2010 韓國菌學會誌 Vol.38 No.2

The contents of proximate composition, free amino acids and phenolic acids in the Fomitella fraxinea cultivated-Rhus verniciflua stem bark(FRVSB), and its adipogenesis effect were investigated. The proximate composition(%) of FRVSB was as follows: moisture(7.64), ash(6.30), crude fat(3.86), crude protein(3.59) and sugar(not detected); while Rhus verniciflua stem bark(RVSB) contained 1.64, 8.09, 7.28, 6.48 and 5.39, respectively. The total free amino acids concentration was 97.41 mg% in FRVSB and 71.91 mg% in RVSB. Phosphoserine(55.06 mg%), ammonia(17.84mg%) and aspartic acid(6.05mg%) were predominant amino acids. The content of total phenolic acids was 422.89 ppm/ in ethanol extract and 283.86 ppm (in water extract), with syringic and gallic acid as the main component. The FRVSB extracts showed a potent free radical scavenging activity for DPPH(2,2-diphenyl-1-picrylhydrazyl hydrate) with IC50 of 28.54 μg(EtOH) and 54.70 μg(water), respectively, whereas IC50 value of gallic acid was 1.84 μg. The protective effect of both ethanol and water extract the extracts against UV-induced oxidative stress in NIH3T3 was observed. The water extracts of FRVSB may promote adipogenesis in 3T3-L1 cells.

RANKL 억제제의 최신 업데이트

최한석 ( Han Seok Choi ) 대한내과학회 2018 대한내과학회지 Vol.93 No.3

The prevalence of osteoporosis is continuing to increase with growing elderly population. A variety of anti-osteoporotic agents have been used for the prevention and treatment of osteoporosis and osteoporotic fractures. Novel therapeutic agents based on the newly discovered mechanisms in bone biology have been recently introduced. Denosmab, a fully human monoclonal antibody to the receptor activator of nuclear factor-κB ligand, is a new therapeutic agent that inhibits osteoclast differentiation, activity, and survival. In the fracture reduction evaluation of denosumab in osteoporosis every 6 months (FREEDOM) trial, denosumab treatment for 36 months significantly increased bone mineral density at all skeletal sites evaluated, thereby resulting in a significant reduction in the risk of vertebral, nonvertebral, and hip fractures. In the FREEDOM Extension study, in which denosumab treatment was continued for up to 10 years, denosumab showed a continued improvement in bone mineral density and a consistently low fracture risk similar to rates observed in the denosumab group during the FREEDOM trial. Denosumab also offered a favorable safety profile with generally low and stable adverse event rates. Denosumab was indicated for treatment of postmenopausal women with osteoporosis at high risk for fracture, treatment to increase bone mass in men with osteoporosis, treatment of bone loss in men receiving androgen deprivation therapy for prostate cancer, and treatment of bone loss in women receiving adjuvant aromatase inhibitor therapy for breast cancer. For these patients, denosumab is an important option for the prevention and treatment of osteoporosis and osteoporotic fractures. (Korean J Med 2018;93:252-259)