일차배양 간세포에서 t-Buty 1hydroperoxide에 의해 유발된 산화적 스트레스에 대한 길경 열수 추출물의 보호효과

최철웅(Chul Yung Choi),이경진(Kyung Jin Lee),정혜광(Hye Gwang Jeong) 대한약학회 2002 약학회지 Vol.46 No.6

Oxidative stress is considered to be associated with many diseases, such as inflammatory and cardiovascular diseases, aging and cancer. An important etiological mechanism of these diseases may be a causal relationship between the presence of oxidants and the generation of lipid hydroperoxides derived from enzymatic reactions or xenobiotic metabolism. The hydroperoxides can be decomposed to alkoxy- (RO·) and peroxy- (ROO·) free radicals that can oxidize other cell components, resulting in changes in enzyme activity or the generation of mediators, which can cause further cell damage. The aim of this study was to evaluate the ability of aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil (CK), to affect cellular response in primary cultures of rat hepatocytes to t-butyl hydroperoxide (t-BHP) induced oxidative stress and hepatotoxicity. CK-treated cells showed an increased resistance to oxidative challenge, as revealed by a higher percent of survival capacity In respect to control cells. CK reduced t-BHP-enhanced lipid per- oxidation measured as production of malondialdehyde and enhanced intracellular reduced glutathione depletion by t-BHP. Furthermore, CK protected from the t-BHP-induced intracellular generation of reactive oxygen species assessed by monitoring dichlorodihydrofluorescein flurorescence. It can be concluded that CK exerts an antioxidant action inside the cell, responsible for the observed modulation of the cellular response to oxidative challeng, and CK have a markd antionxidative and hepatoprotective potency.

건축물의 구성재에 따른 쥐성장 생활환경과 체중 증감효과에 관한 실험적 연구

정영배(Yung-Bea Chung),김미옥(Mi-Ok Kim),최철웅(Chul-Yung Choi) 한국실내환경학회 2006 한국실내환경학회지 Vol.3 No.4

In architecture, constitutions of space are subjected to the structural material and interior finishing material. Interior environment can exert a great effect on people by means of space constitution. In this study the constitution of space was set to consider five materials: control, wood, steel, wire mesh, and concrete structural material. In this study, experiments with mice, were carried out on condition of each living environment with different living environment using control, wood, steel, wire mesh and concrete structural material. The experiments with mice were made by five-month-old mice(BALB/C) with about 15 grams weight. In order to evaluate the adaptability of the target mice, such variables as body weight, food consumption, and water consumption were monitored against living environment for normal BALB/C mice during the same period. The weight, total foods, and total water consumption were measured under the living environments for 55 days with 2~3 days intervals. To test five different living environments, i.e., control, wood, steel, wire mesh and concrete, 5 mice were allocated for each group. According to the results of this study, the case of wood living environment resulted in a gain in weight with statistical significance. The other side of steel, wire mesh and concrete showed a slight decrease for weight. The weight variation of the experimental mice was assumed under five different living environments that resulted from the stabilized environmental conditions such as the wood living environment.

뇌하수체 세포인 GH3세포에서 non-genomic action을 통한 Nonylphenol의 nitric oxide 증진효과

이경진(Kyung-Jin Lee),최철웅(Chul-Yung Choi),손현정(Hyun-Jung Sohn),정백진(Back-Jin Jeong),문소희(So-Hee Moon),이황희(Hwanghee Blaise Lee),이종빈(Jong-Bin Lee) 환경독성보건학회 2003 환경독성보건학회지 Vol.18 No.4

본 연구는 환경호르몬(endocrine disruptors)으로 분류되었으며,에스트로젠 화합물의 특성을 지닌 4-Nonylphenol(NP)이 설치류 pituitary 세포 중 성장호르몬을 분비하는 GH3 세포의 Nitric oxide (NO)을 증가시키는 작용기전을 규명코자 수행되었다. 먼저 GH3세포에 NP처리 농도에 따른 NO의 생성을 측정한결과 NP처리농도 의존적으로 증가시켰다. 이러한 NO의 증가가 genomic action인지를 확인하기 위해GH3 세포의 NO를 증가시키는 효소인 neuronal oxide synthase의 단백질량을 측정한 결과 CH3 세포에서NP에 의한 nNOS의 단백질의 변화는 없었다. 에스트로젠 화합물인 NP가 에스트로젠 리셉터 (ER)와의관계를 조사하기 위해 ER억제제(ICI 168,780)를 처리한 경우 NP에 의해 증가한 NO가 감소하였다. 또한유전자 전사억제제인 actlnomycin D 및 단백질 발현 억제제인 cycloheximide을 처리한 경우는 NP에 의한NO 증가억제효과가 없었다. 이러한 결과를 종합해 볼 때 GH3 세포에서 NP는 ER을 매개한 non-genomicaction에 의해 NO를 증가키는 것으로 사료된다.

천연수액으로 제조한 간장의 품질특성

조숙현 ( Sook Hyun Cho ),최용조 ( Yong Jo Choi ),오주열 ( Joo Yeul Oh ),김낙구 ( Nak Gu Kim ),노치웅 ( Chi Woong Rho ),최철웅 ( Chul Yung Choi ),조성환 ( Sung Hwan Cho ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2007 한국식품저장유통학회지 Vol.14 No.3

갈근 및 녹차추출물의 아질산염 소거작용 및 간독성 보호효과

윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),허정호 ( Jung Ho Heo ),최철웅 ( Chul Yung Choi ),성태종 ( Tae Jong Seoung ),김윤근 ( Yun Geun Kim ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3

This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/ 100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~ 100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of galgun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.

대나무수액의 활성산소 소거활성과 세포독성

조숙현 ( Sook Hyun Cho ),최용조 ( Yong Jo Choi ),노치웅 ( Chi Woong Rho ),최철웅 ( Chul Yung Choi ),김덕송 ( Deok Song Kim ),조성환 ( Sung Hwan Cho ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2008 한국식품저장유통학회지 Vol.15 No.1

멀꿀 열매 추출물의 항산화 활성 및 H₂O₂로 유도된 산화적 스트레스와 아세트아미노펜 독성 모델에서의 간 보호효과

이규옥(Gyuok Lee),김재용(Jaeyong Kim),강후원(Huwan Kang),배동혁(Donghyuck Bae),최철웅(Chul-yung Choi) 한국생명과학회 2018 생명과학회지 Vol.28 No.6

본 연구에서는 멀꿀 열매 추출물의 세포수준에서 항산화 활성과 아세트아미노펜(APAP)으로 유도된 간 독성 동물모델에서의 간 기능 보호효과를 연구하였다. 멀꿀 열매 추출물의 총 폴리페놀 함량과 플라보노이드 함량은 각각 16.13±0.27 mg gallic acid equivalent/g 및 4.7±0.80 mg catechin equivalent/g이었다. 또한 DPPH 라디컬소거능과 ORAC도 각각 63.62±4.10 μg/ml 및 90.63±5.29 μM trolox equivalent/g이었다. 과산화수소로 유도된 산화적 손상에 대한 멀꿀 열매 추출물의 간세포 보호효과를 실험한 결과, 200 μg/ml에서 세포생존율이 증가하고 증가된 LDH 활성이 감소함을 확인하였다. 간세포에서 과산화수소로 산화적 스트레스를 유도하여 감소된 항산화효소(SOD, CAT, GR, GPx)의 활성은 멀꿀 열매 추출물 처리로 활성이 증가하여 간세포를 보호하였다. 아테트아미노펜(APAP) 유도 간 손상 생쥐 모델에서 간 보호활성을 평가하였다. 간 손상 혈청 표지지표인 ALT 및 AST수준이 APAP 단독 처리군에 비해 멀꿀 열매 추출물 200 mg/kg군에서 유의적으로 감소되었으며, 간의 과산화지질함량도 감소한 것으로 나타났다. 간 조직의 병리학 검사에서도 간조직이 정상 회복되는 형태를 보였다. 이상의 결과를 토대로 멀꿀 열매 추출물이 항산화 기전을 통해 간기능 보호 효과를 나타냄을 확인할 수 있었다. The antioxidant activity and protective effects of a hot water extract from the Stauntonia hexaphylla fruit (WESHF) were investigated in vitro and in vivo. The total polyphenol and flavonoid contents of WESHF were 16.13±0.27 mg gallic acid equivalent/g and 4.7±0.80 mg catechin equivalent/g, respectively. In addition, the DPPH radical-scavenging activity (SC50) and the Oxygen Radical Absorbance capacity of WESHF were 63.62±4.10 μg/ml and 90.63±5.29 μM trolox equivalent/g, respectively. The hepatoprotective effect of WESHF against hydrogen peroxide-induced oxidative damage was investigated. H₂O₂-induced liver damage on HepG2 cells was prevented by 200 μg/ml of WESHF. Furthermore, to investigate the protection mechanism of WESHF on hydrogen peroxide-induced cytotoxicity in HepG2 cells, pre-treatment with 200 μg/ml of WESHF significantly attenuated a decrease in the activities of CAT, SOD, GR, and GPx. The hepatoprotective activity of WESHF was evaluated in an experimental model of hepatic damage induced by acetaminophen (APAP). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly decreased in the livers of mice treated with 200 mg/kg of WESHF compared to the APAP-treated group. The lipid peroxidation level, which increased after APAP administration, was significantly reduced in the WESHF group. In addition, histological examinations of the liver showed the same protective effect of WESHF treatment. Based on these findings, it is suggested that WESHF has potent hepatoprotective effects, and the mechanism that causes this type of protection could be related to antioxidant pathways.

실험쥐를 이용한 식생활 및 주거환경의 민감도에 대한 기초적 연구

김미옥 ( Mi Ok Kim ),은종극 ( Jong Geuk Eun ),정영배 ( Yung Bea Chung ),최철웅 ( Chul Yung Choi ) 한국식품영양학회 2008 韓國食品營養學會誌 Vol.21 No.1

건강기능식품 기능성 원료로써 장흥 차조기 열수 추출물의 지표성분인 로즈마린산 분석법 검증

박성용(Sung-Yong Park),김정은(Jung-Eun Kim),최철웅(Chul-Yung Choi),이동욱(Dong-Wook Lee),김기만(Ki-Man Kim),윤구(Goo Yoon),윤인수(In-Su Yoon),문홍섭(Hong-Seop Moon),조승식(Seung-Sik Cho) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.1

차조기 열수 추출물을 개별인정형 건강기능식품 기능성 원료로 개발하기 위하여 지표성분 표준화를 위한 rosmarinic acid의 분석법 설정과 분석법에 대한 밸리데이션을 실시하였다. 1% acetic acid가 첨가된 water와 methanol을 이동상으로 하고 Zorbax extended-C18(4.6×150 mm, 5 μm) 칼럼을 사용하여 기울기 용리(gradient elution) 방법으로 분석하였다. 본 연구에서는 분석법을 확립하고 분석법에 대하여 특이성, 직선성, 정확성과 정밀성 그리고 회수율에 대하여 확인하였다. Rosmarinic acid의 검량선은 R2=0.9995로 좋은 선형성을 보였으며 정량한계와 검출한계는 각각 1.2 μg/mL와 0.36 μg/mL였다. 일내와 일간 분석에서 상대 표준편차(RSD)는 각각 3.21%와 1.43% 미만으로 나왔다. 정밀성 측정 결과에서는 97.04~98.98%로 나왔고, RSD는 0.25~1.97%였다. 그러므로 HPLC를 이용한 rosmarinic acid의 분석법이 차조기 열수 추출물 기능성원료 표준화를 위한 적합한 시험법임이 검증되었다. 본 시험법에 따라 분석한 차조기 열수 추출물 내의 rosmarinic acid의 함량은 세 lot를 3회 분석하였을 때 평균 0.03%로 나왔다. 따라서 본 연구를 통하여 확립된 rosmarinic acid의 분석법이 차조기 열수 추출물 개별인정형 건강기능식품 기능성 원료 개발을 위한 기초자료로 활용될 것으로 사료된다. This study attempted to establish an HPLC analysis method for determination of marker compounds as a part of material standardization for the development of health functional food materials from Perilla frutescens Britton var. acuta Kudo. The quantitative determination method of rosmarinic acid as a marker compound of P. frutescens Britton var. acuta Kudo extract (PFE) was optimized by HPLC analysis using a C18 column (4.6×150 mm, 5 μm) with 0.1% acetic acid as the elution gradient and methanol as the mobile phase at a flow rate of 1 mL/min and detection wavelength of 280 nm. The HPLC/UV method was applied successfully to quantification of the marker compound in PFE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation (R2) of 0.9995, and the limit of detection and limit of quantitation were 0.36 μg/mL and 1.2 μg/mL, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 3.21% and 1.43%, respectively. Recovery rate test at rosmarinic acid concentrations of 12.5, 25 and 50 μg/mL scored between 97.04~98.98% with RSD values from 0.25~1.97%. These results indicate that the established HPLC method is very useful for the determination of marker compound in PFE to develop a health functional material.

아세트아미노펜에 의해 유도된 간독성 모델에서의 Theanine의 간보호 효과

우정부(Jung Bu Eu),김선오(Sun Oh Kim),성태종(Tae Jong Seoung),최성길(Sung Gil Choi),조성환(Sung Hwaon Cho),최철웅(Chul Yung Choi) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.3

아세트아니모펜(APAP)으로 유도된 간독성 모델에 미치는 theanine의 간 보호 작용에 대하여 간 기능 지표효소의 활성 측정, 항산화 및 GSH량 측정, 조직학적 변화 등을 통해 확인하였다. Theanine은 그 자체로 항산화 효과를 보였으며, 과량 투여된 APAP에 의해 발생하는 간조직의 지질과산화의 감소와 GSH가 회복되는 것을 동물실험을 통해 확인할 수 있었다. 또한 지질과산화와 GSH의 감소로 인해 발생한 간세포의 손상이 theanine의 처리 농도에 비례하여 감소하는 것을 간수치 검사와 간조직 검사를 통해 최종 확인하였다. 지금까지 간보호에 효과를 보이는 녹차의 카테킨이 주로 연구되어 왔으나, 본 연구를 통해 녹차의 theanine도 간 보호 작용을 알 수 있었다. 이러한 연구 결과는 주 아미노산 성분인 theanine을 강화시킨 기능성 녹차 및 건강기능식품 개발의 기초 자료로 활용 가능할 것으로 사료된다. The hepatoprotective effects of theanine on acetaminophen (APAP)-induced hepatotoxicity were investigated in vivo and in vitro. The effects of theanine on liver toxicity induced by APAP were assessed by blood biochemical and histopathological analyses. APAP treatment (400 mg/kg) caused severe liver injury in mice as indicated by their significantly elevated plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Pretreatment with theanine for 3 days attenuated the increase in ALT and AST when challenged with APAP. These protective effects of theanine against APAP-induced toxicity were consistent with the results from the histopathological examinations. We next examined the effects of theanine on the GSH concentration in liver plasma. The hepatic GSH level was significantly elevated in a dose-dependent manner by theanine treatment. The results suggest that the protective effects of theanine APAP-induced hapatotoxicity by antioxidative effect and GSH induction, implying that theanine should be considered a potential chemopreventive agent.