Antioxidant and Anticancer Effects of Millet and Sorghum Varieties

Ji Myung Choi(최지명),Jee Yeon Ko(고지연),Seok Bo Song(송석보),Myeong Eun Choe(최명은),Chang Hwan Park(박장환),Do Yeon Kwak(곽도연) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

아밀로이드 베타에 의해 유도된 인지 및 기억능력 손상에 대한 김치의 보호 효과

최지명(Ji Myung Choi),이상현(Sanghyun Lee),박건영(Kun Young Park),강순아(Soon Ah Kang),조은주(Eun Ju Cho) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.3

김치의 EtOH 추출물을 이용하여 in vitro 상에서 DPPH와 ?OH radical 소거능을 측정하였고, ICR mouse의 해마 부위에 Aβ를 주입시킨 AD model을 이용하여 김치 추출물을 2주간 투여한 후 물체 인지, T-maze, water maze의 실험방법을 이용하여 인지능력 개선 효과를 살펴보았다. 김치추출물은 우수한 DPPH와 ?OH radical 소거능을 나타내었다. 또한 AD 동물 model에서 해마 부위에 Aβ를 주입한 control군의 경우 물체 인지, 기억 및 학습능력의 손상을 확인할 수 있었으나, 김치 추출물을 100과 200 mg/kg/day 를 투여한 군에서는 물체 인지 실험에서 새로운 물체에 대한 호기심 정도가 높았으며, T-maze 실험에서는 새로운 길에 대한 탐색 정도도 뛰어난 것을 확인할 수 있었다. Water maze 실험에서도 도피대를 찾아가는 반복 훈련을 할수록 도피대를 찾아가는 시간이 점차 단축되는 것을 확인할 수 있었으며, 도피대를 기억하는 능력도 향상됨을 확인할 수 있었다. 본 연구 결과에서는 김치 추출물이 radical을 소거함으로써 산화적 스트레스로부터 보호하여 인지능력 및 기억능력을 향상시키는 효과가 있음을 확인하였다. 이러한 연구를 바탕으로 김치의 산화적 스트레스 개선 효과 및 AD예방에 대한 상관관계에 대한 작용기작 연구가 이루어진다면 우리나라 대표 식품인 김치의 섭취로 인한 AD 예방 효과에 대해 명확하게 규명할 수 있을 것으로 사료되어진다. Kimchi is a Korean traditional fermented food with various health functionalities. However, the protective effects of kimchi against Alzheimer"s disease (AD) have not been studied yet. In this study, the protective activities of kimchi extract against oxidative stress and AD were investigated in an amyloid beta (Aβ)-induced AD model using ICR mice. Kimchi extract exerted strong scavenging activities against 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical. In addition, T-maze, object cognition, and water maze tests were carried out using the AD model. The Aβ25-35-injected groups showed impairment of cognition and memory. However, the abilities of novel object recognition and new route awareness were improved by administration of kimchi extract (100 and 200 mg/kg/day) for 2 weeks. Furthermore, the results on water maze test indicated that kimchi extract exerted protective activity against cognitive impairment induced by Aβ25-35. The present study suggested that kimchi protected against Aβ-induced impairment of memory and cognition as well as attenuated oxidative stress.

흰민들레(Taraxacum coreanum) 활성획분인 Ethylacetate Fraction의 Peroxynitrite에 대한 산화적 스트레스 개선 효과

최지명 ( Ji Myung Choi ),최미진 ( Mi Jin Choi ),이설림 ( Sul Liim Lee ),야마베노리꼬 ( Noriko Yamabe ),이상현 ( Sang Hyun Lee ),조은주 ( Eun Ju Cho ) 부산대학교 김치연구소 2012 김치의 과학과 기술 Vol.15 No.-

The EtOAc fraction from Taraxacum coreanum showed the strongest radical scavenging activity. The protective activity of active EtOAc fraction against the oxidative stress was investigated under cellular system using LLC-PK1 renal epithelial cell. The LLC-PK1 cells showed loss of the cell viability and elevation for lipid peroxidation by the treatment with generator of peroxynitrite (ONOO-), 3-mor- -pholinosydnonimine, and its precursors, nitric oxide (NO) and superoxide anion (O2 ), produced by sodium nitroprusside and pyrogallol, respectively. However, the treatment of the EtOAc fraction significantly, dose-dependently, recovered cell viability and inhibited lipid peroxidation against both ONOO- itself, and its precursors of NO and O2 . At 100μg/ml of the EtOAC fraction, the cell viability was significantly increased to 93.4% from 61.0%, and lipid peroxidation was inhibited to 0.104 nmol/mg protein from 0.494 nmol/mg protein. The present study suggests that T. coreanum would have the protective potential from ONOO--induced oxidative stress.

논 재배 팥 품종별 수량구성요소 및 에탄올 추출물의 항산화 성분 비교

전현정,정기열,최영대,이상훈,송석보,고지연,최지명,장윤우,Chun, Hyen Chung,Jung, Ki Yuol,Choi, Young Dae,Lee, Sanghun,Song, Seok bo,Ko, Jee Yeon,Choi, Ji Myung,Jang, Yun Woo 한국작물학회 2017 한국작물학회지 Vol.62 No.3

This study investigated the changes in yields, antioxidant compounds, and antioxidant activities among 11 adzuki bean cultivars cultivated in a paddy field with somewhat poorly drained soil. The adzuki bean cultivars were cultivated in the paddy field from 2015 to 2016 in Milyang, Gyeongsangnam, Korea. Average soil moisture content was 16.5% in 2015 and 31.0% in 2016 at the experimental site during adzuki bean cultivation. As the soil moisture content increased, most of the adzuki bean cultivars showed deceases in stem height, first setting pod node, number of pods, 100 seed weight, and yield. Chungju-pat produced the greatest yields among the 11 cultivars in 2015 and 2016, whereas Hongeon had the smallest yields. Adzuki bean yields after paddy field cultivation was lower for all cultivars than for adzuki bean yields from the same cultivars after upland field cultivation. Chungju-pat and Chilbo-pat showed the smallest decreases in yields after paddy field cultivation, whereas Hongeon and Kumsil showed the greatest yield reductions. There were no significant differences in proximate composition. Some mineral components ($P_2O_5$, Ca, and Mg) were statistically different across cultivars. Chungju-pat had the highest Ca and Mg contents, but antioxidant components (polyphenol and flavonoids) and antioxidant activities (ABTS and DPPH) were highest in Saegil and lowest in Jungbu-pat. PCA and clustering analyses, based on the growth, yield, and antioxidant component measurements, performed to identify which variables contributed the most to separating adzuki bean cultivars or to grouping cultivars with similar characteristics. These analyses showed that the antioxidant components and antioxidant activities had the most influence on grouping cultivars together. Among the 11 cultivars, Saegil was statistically different from the other cultivars, but the other 10 cultivars were not significantly different under paddy field cultivation. Soil moisture content affected adzuki bean yield and antioxidant component contents. An increase in soil moisture led to a decrease in yield, but an increase in antioxidant components. These results provide information that will improve the selection of an appropriate adzuki bean cultivar for use in paddy fields.

국내산 법제 하수오의 라디칼 소거능 및 산화적 스트레스 개선 효과

김현영(Hyun Young Kim),김준영(Jun Young Kim),조은주(Eun Ju Cho),최지명(Ji Myung Choi),황정은(Chung Eun Hwang),이희율(Hee Yul Lee),안민주(Min Ju Ahn),이진환(Jin Hwan Lee),김윤근(Yun-Geun Kim),고건희(Keon Hee Ko),구영민(Young-Min Goo) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.6

본 연구에서는 국내산 법제 하수오 메탄올 추출물로부터 생리활성물질을 확인하였고, 추출물의 라디칼 소거능과 LLCPK1 cell을 이용한 산화적 스트레스 개선 효과를 살펴보았다. HPLC 분석 결과 4종의 생리활성물질인 2,3,5,4"-tetrahydroxystilbene 2-O-β-D-glucoside, emodin, chrysophanol 및 rhein을 확인하였다. 특히 주요 화합물인 2,3, 5,4"-tetrahydroxystilbene 2-O-β-D-glucoside는 115.02 mg/kg이었다. 법제 하수오 추출물의 DPPH, ABTS, hydroxyl 라디칼 소거능은 농도 의존적으로 증가하였다. 추출물의 50 μg/mL 처리 시 DPPH 라디칼 소거능은 48.4%, ABTS 라디칼 소거능은 57.9% 및 hydroxyl 라디칼 소거능은 81.2%로 나타내었다. 한편 LLC-PK1 cell에서 각각의 NO, O₂-, 및 ONOO- 생성물질인 pyrogallol, sodium nitroprusside(SNP) 및 morpholinosydnonimine(SIN-1) 처리에 의해 유도된 산화적 스트레스 상에서 세포 생존율은 감소하였다. 그러나 법제 하수오 메탄올 추출물은 농도 의존적으로 세포 독성을 저해하였다. 추출물의 50 μg/mL 처리 시 세포 생존율은 각각 82.1%(pyrogallol), 89.1%(SNP) 및 77.6%(SIN-1)였다. In this study, we confirmed biological compounds from methanol (MeOH) extract of processed Polygoni Multiflori Radix (PPMR), and the radical scavenging effect and oxidative stress protective activity of MeOH extract of PPMR were investigated under in vitro conditions using LLC-PK1 renal epithelial cells. In HPLC analysis, MeOH extract of PPMR contained four species of biological compounds named 2,3,5,4"-tetrahydroxystilbene 2-O-β-D-glucoside, emodin, chrysophanol, and rhein. 2,3,5,4"-Tetrahydroxystilbene 2-O-β-D-glucoside was detected as the main compound in PPMR as 115.02 mg/kg. MeOH extract of PPMR showed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2"-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS), and hydroxyl radical scavenging activities in a concentration-dependent manner. In particular, upon 50 μg/mL of PPMR extract treatment, DPPH, ABTS, and hydroxyl radical scavenging activities were approximately 48.4%, 57.9%, and 81.2%, respectively. LLC-PK1 cell viability declined in response to oxidative stress induced by pyrogallol, sodium nitroprusside (SNP), and morpholinosydnonimine (SIN-1) generators of NO, O₂-, and ONOO-, respectively. However, MeOH extract of PPMR significantly and dose-dependently inhibited oxidative-stressed LLC-PK1 cell cytotoxicity. In fact, upon 50 μg/mL of PPMR extract treatment, LLC-PK1 cell viabilities were approximately 82.1%, 89.1%, and 77.6% compared to stress levels induced by pyrogallol, SNP, and SIN-1, respectively.