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原著(원저) : 혈장성분헌혈의 혈장 채혈량 조정을 위한 실험
고중석 ( Joong Seok Koh ),최범열 ( Bum Ryoul Choi ),이충영 ( Chung Young Lee ),김종훈 ( Jong Hoon Kim ),정창숙 ( Chang Sook Jeong ),최중문 ( Joong Moon Choi ) 대한임상병리사협회 2001 임상수혈검사학회 발표자료집 Vol.6 No.1
배경 : 1회 혈장 헌혈량을 항응고제가 포함된 500mL와 순수 혈장 500mL(항응고제 포함 500mL 이상)로의 이원화 필요성이 제기되고 있다. 혈장 성분 채혈에 있어서 순수 혈장량 500mL를 도입하기 전에 기존 채혈량 500mL를 채혈하는데 따른 차이를 비교하여 헌혈자의 안전을 확보하기 위해 본 실험을 실시하였다. 방법 : 제 I군은 항응고제를 포함해서 500mL의 혈장을 채집한 남성 115예, 여성 78예로 하였고, 제 II군는 헌혈자의 동의를 받아 순수 혈장 500mL(항응고제 포함 500mL 이상) 채집한 남성 108예, 여성 62예로 하였다. 헌혈자 두 군의 헌혈 전, 후 혈압, 맥박과 Hct., 혈장 총단백을 측정하였고, 각 성분채혈기별 비교와 헌혈자에서 나타난 부작용, 혈장제제 1단위당 신선 혈장의 양 등을 비교하였다. 또한, 혈장 헌혈 때 드물게 발생할 수 있는 부작용은 혈장성 분헌혈 시행 과정 또는 후에 관찰하였다. 결과 : 1. 제 I 과 제 II군의 두 군 사이의 연령과 남성 체중의 유의한 차이는 없었으며, 헌혈 전, 후의 혈압, 맥박, Hct.와 총단백량도 유의한 차이를 보이지 않았다(p>0.05). 2. 성분채집기 3종을 중심으로 두 집단간의 헌혈후 각종 모니터 수치를 비교해 보았는데 체외순환 혈액량, 항응고액과 혈장량, cycle 및 소용시간 등에서 유의한 차이를 보였다(p<0.05). 3. 두 군 모두에서 특별한 헌혈 부작용은 없었다. 결론 : 국내 혈장 자급자족에도 도움이 되며, 헌혈자에게도 특별한 부작용이 나타나지 않는 점을 고려해 볼 때, 혈장 채혈량을 항응고액 포함 500mL 용량과 순수 혈장 500mL 용량(항응고제 포함 500mL 이상)으로 하는 이원화 방안을 제시해 본다. Background : It has been proposed that the amount of donated plasma should be differentiated according to the volume of plasma collected ; 500mL of donated plasma including anticoagulant versus 500mL of pure plasma (over 500mL, when it includes anticoagulant). The purpose of this study was to evaluate whether the increase of donated plasma volume would have any adverse effects on donor safety. Methods : According to the volume of plasma collected donor w r divided into group I (500mL of plasma including anticoagulant) and group II (500mL plasma only; more than 500mL if anticoagulant is included). Group I and II comprised 115 and 108 men and 78 and 62 women, respectively. Pre- and post-plasmapheresis of blood pressure, pulse, hematocrit, and total protein were compared between the two group. In addition, parameter of apheresis instruments used and occurrence of adverse reactions du to increased plasma collection were evaluated. Results : 1. Regarding age, body weight of male donors, and pre- and post-plasmapheresis of blood pressure, pulse, hematocrit and total protein, no significant difference was observed between the two groups (p>0.05). 2. Parameters of apheresis instrument (extracorporeal
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원저(原著) : 한국인 헌혈자에서의 C형간염항체 양성률 고찰 -제3세대 효소면역측정법 및 확인시험-
최범열 ( Bum Ryoul Choi ),최중문 ( Joong Moon Choi ),김혜경 ( Hye Kyoung Kim ),김석완 ( Seoung Wan Kim ),민태희 ( Tai Hee Min ) 대한임상병리사협회 1995 임상수혈검사학회 발표자료집 Vol.2 No.1
After test the blood samples donated by 33,838 Koreans with Lucky HCD, 3rd generation Hepatitis C diagnostic reagent, using ELISA method, We selected the samples found positive and retest them with Lucky HCD confirm, which is the diagnostic reagent confirming Hepatitis C in Immunoblot method and achieved the following results. 1. Among blood doner 33,838, 133 which is equivalent to 0.39 % of the total were found anti-CV positive. Among the male, 31,023(92 %), 126 which is equivalent to 0.41 % were found positive. Among the female 2.815(8 %), 7 which is equivalent to 0.25 % were found positive. 2. When tested 133 which were found positive by ELISA method with Lucky HCD confirm in order to confirm the test results, 40(30 %) were found positive, 93(70 %) were false positive, 17(3 %) were indeterminate, and 76(57 %) were negative. Summarizing from the total blood donors, 0.12 % were anti-HCV and the false positive percentage except indeterminate were 0.22 %. 3. The ALT value of 32,159(95 %) of the total blood donors were less then 45IU/L(normal). When 32,159 were tested with HCD3.0, 109(0.34 %) were found anti-HCV. When 109 were rested with Lucky confirm, 24(0.07 %) were found positive. 771(2.3 %) of the total blood donors were having ALT value from 46 to 59 IU/L. When 771 were tested with HCD3.0, 8 were found positive. When 8 were retested with Lucky confirm, 3(0.39 %) were found positive. 908(2.7 %) of the total donors were having ALT value above 60IU/L. When 908 were tested with HCD 3.0, 16(1.76 %) were found positive. 16 were retested with Lucky confirm, and 13(1.43 %) were found positive. These statistics show that the potential percentage of HCV positive grows in the proportion of the ALT value. As shown above, the substantial quantity of the blood donated are presently decided false positive by the Hepatitis C Antibody test using 3rd generation ELISA method in the Red Cross Blood Center and destroyed. By noticing such results to the blood donors having goodwill, The Red Cross might lose it`s faith for testing the blood and it will eventually affect the controlling the blood donors To supplement the current testing system, we consider that we need to have final confirm test at the end of the test procedure before release the test results.
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원저(原著) : 친자확인 검사에서 발견된 cis-AB가족 1예
최범열 ( Bum Ryoul Choi ),이동범 ( Dong Beom Lee ),최중문 ( Joong Moon Choi ),성호연 ( Ho Yun Sung ) 대한임상병리사협회 1996 임상수혈검사학회 발표자료집 Vol.3 No.1
저자들은 일상 혈액형 검사에서 혈구형 검사만을 실시할 경우 발견할 수 없었던 cis-A2B3형 아버지와의 A1형 어머니 사이에서 O형의 아들이 출생된 cis-AB형 가계를 경험하였기에 문헌고찰과 함께 보고하며 수혈검사시 ABO식 혈액형에서 필히 혈구형과 혈청형을 실시하여야 할 것임을 제안하고자 한다. ABO discrepancy is difficult to inspections of one`s child confirmation and transfusion. Cause of ABO discrepancy have several of things and the authers introduced 1 case of the causes to you. ABO blood group is determinated by A and B genes located on each chromosomes which are inherited from parents.But cis-AB has been reported unusual inheritive of A and B genes on the same chromosome. By a routine blood typing, we have found that parent of cis-A2B3 type and A1 type can bear children of O type blood. From the fact above-mentioned, we have concluded that the above case cannot be predicted from Mendel`s laws. Therefore, We intend to report the truth with the conference books and propose that medical technologist must be tested cell and back typing certainly pretransfusion test.
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