남성유방에 발생한 지방육종

최종범,임성철,기근홍,전호종,서재홍 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.3

다형성 선종에서의 근상피세포에 대한 병리학적 연구

최종범,임성철,전호종,서재홍 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.2

흰쥐에서 간담관 장애가 신장에 미치는 초미세 형태학적 연구

김용임,김일,이길부,최종범,기근홍,김용일,서재홍 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

It has been well-known that biliary cirrhosis with concomittant impairment of renal functions were occured after prolonged obstruction of biliary tract. In the present study, an attempt was made to investigate the mechanism of impaired renal function and the ultrastructural change of the kidney in rats, ligated the common bile duct for 21, 28 and 50 days in order to induce secondary biliary cirrhosis. The results obtained were as follows ; 1. Secondary biliary cirrhosis was produced evidently 28 days after ligation of common bile duct. Prominent proliferations of bile ducts and chronic inflammatory cell infiltrations were seen in pehportal area. 2. 28 and 50 days after ligation of common bile duct, show swelling and degeneration of mitochondria, microcystic formation and lysis of endoplasmic reticulum in the hepatocytes. Loss of microvilli in the bile ductules were noted. 3. Focal fusions, edema and bleb formations of foot processes were seen. Electron dense materials with variable size and global shape were found in foot process, 21, 28 and 50 days after ligation of common bile duct. 4. There were slightly thickening of renal glomerular basement membrane with hump in the subendothelial and widening of the mesangial matrix, 28 days after ligation of common bile duct. 5. There were moderately thickening of renal glomerular basement membrane and widening of the mesangial matrix with electron dense deposits, 50 days after ligation of common bile duct. From the above findings of electron microscopy, this study revealed electron dense deposits on renal glomerular mesangium of the rats as a results of experimental secondary biliary cirrhosis by ligation of common bile duct. Also severe hepatic injury due to swelling or lysis of rough endoplasmic reticulum and destruction of bile ductules resulted in deposited electron dense materials due to inhibition of excretion of immune complex through hepatobiliary tract, therefore this change indicated that immuncomplex could be deposited on renal glomerular mesangium.

O,p'-DDD가 흰쥐의 신장에 미치는 독성에 관한 면역조직화학적 및 초미세형태학적 연구

배재웅,최종범,임성철,김균홍,기근홍,박동수,전호종,박규호 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

o,P'-DDD, a derivative of insecticide DDT, which reveals the toxicity and adrenostatic effet by cortisol biosynthesis in the zona fasciculata of adrenal cortex has been used for the treatment of unoperable adrenocortical carcinoma. Adverse effects of o,P'-DDD were usually dose-related and reversible. Most untoward effects include gastrointestinal disturbances, neuromuscular disorders, depression of central nervous system and skin rash but there is a few reports on its nephrotoxicities. o,P'-DDD in corn oil was administered orally to rats for 28 days to investigate its nephrotoxicity by observation of the ultrastructural changes and immunoglobulin deposition in renal glomeuli. The results obtained were as follows. 1. The ultimate destructive target of intracytoplasmic organelle in endothelium and epithelium of renal glomeruli was mitochondria. The severity of the mitochondrial alterations was dependent of the dosages of o,p'-DDD. 2. The characteristic ultrastructural changes in endothelium of glomerular capillary were swelling and detachment from underlying basement membrane. The splitting and complete separations between endothelium and basement membrane were observed in more advanced lesions by the increased dosage of administration of o,p'-DDD and basement membrane in capillary wall of renal glomeruli was irregular and partly thickened. 3. Amorphous double ring formation, electron dense deposits and multiple myelin figures in mitochondria were characteristic ultrastructural findings in the epithelium of glomeruli. 4. Ig G and Ig M depositions were clearly observed in the capillary lumen, subendothelial area and epithelial cells of proximal convoluted tubule. These depositions were more prominent by the increased of o,p'-DDD dosage. From the above results it is suggested that o,P'-DDD exhibits nephrotoxicity by mitochondrial destruction in epithelium and endothelium of glomerular capillary and the severity of mitochondrial alterations was dose dependent of o,p'-DDD.

자궁내막의 탈락막세포에 대한 면역조직화학적 및 전자현미경적 연구

윤확,최종범,임성철,기근홍,서재홍 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

Toward the end of the secretory phase, and in some cases of endometrial hyperplasia, the stromal cells become rounded, acquire more cytoplasm, and develop vesicular nuclei with occasional prominent nucleoli. Cytoplasmic borders become somewhat more distinct. These changes become more generalized and advanced, so that the cells have well defined cytoplasmic borders, abundant cytoplasm, and centrally placed vesicular nuclei. This unique mullerian stromal transformation is called decidualization when fully developed, and predecidualization when partially developed. Human decidual cells of early pregnancy, late secretory phase of the normal menstrual cycle and in some cases of hyperplasia were studied immunohistochemically and ultrastructurally with respect to pericellular basement membrane material, cytoplasmic organelles and intermediate filaments. The results obtained were as follows : 1) The endometrial stroma of early pregnancy and late secretory phase of the normal menstrual cycle and in some cases of hyperplasia consisted of three distinct types of decidual cells: large mature cells(204.5±:58.0 ㎛^(2)), intermediate sized cells(64.1±19.6 ㎛^(2)) and small elongated cells(29.8±7.0㎛^(2)). Decidual tissul specimens from early pregnancy consisted mainly of large, mature epithelioid decidual cells individually dispersed in a loose stroma. The small and intermediate decidual cells were seen at the late secretory phase of the menstrual cycle and in endometrial hyperplasia of menopause. 2) The large mature decidual cells showed intense immunostaining for lysozyme, vimentin, α-1-antitrypsin and type Ⅳ collagen. The intermediate sized decidual cells were immunostained weakly for lysozyme, vimentin, α-1-antitrypsin, type Ⅳ collagen. The small cells(predecidualized cells) did not exhibit immunoreactive deposits of lysozyme, α-1-antitypsin and type Ⅳ collagen, except vimentin. 3) Ultrastructurally, clumps of electron dense basement membrane material were present in vicinity to the cell membrane of the large mature decidual cells in early pregnancy. The small cells (predecidual cells) with no basement membrane material and intermediate filament were also observed. In conclusion, the results suggest that three types of decidual cells might represent various stages of the sequential differentiation of stromal cells into decidual cells, as well as that process of decidualization and predecidualization can be characterized morphologically and immunochemically the accumulation of basement membrane material in specific decidual cell subpopulations and acting as a physical barrier to trophoblast invasion.

이차성 담즙성 간경변증이 신사구체에 미치는 영향에 관한 면역조직화학적 및 초미세형태학적 연구

이준상,최종범,임성철,기근홍,박동수,이병래,전호종,서재홍 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

It has been well known that renal functions in patients with prolonged hepatobiliary disease are impaired and also it's pathogenesis is not yet cleared. In the present study, secondary biliary cirrhosis was induced by bile duct ligation for 28 days in rats to investigate the pathogenesis of renal damages by observations of ultrasructural changes and the types of immunoglobulin deposition in renal glomeruli. The results obtained were as follows. 1. Secondary biliary cirrhosis bearing cirrhotic nodules, prominent proliferations of bile ducts and chronic inflammatory cell infiltration in the periportal area was occured on the 28th day after common bile duct ligation. 2. Ig A depositions in the mesangial matrix were appeared 5 days after bile duct ligation Ig G and Ig M were prominently observed in the intracytoplasm of the proximal convoluted tubular epithelium on the 28th day of bile duct ligation. 3. The characteristic ultrastructural features of the glomeruli included irregularly thickened basement membrane, edema and fusion of the capillary endothelium and foot process. 4. Electron dense deposits were shown in the subendothelial, intramembranous and subepithelial areas and in the mesangial matrix. Intramembranous electron dense deposits were enclosed by fine membranous materials while that in the mesangial matrix were irregular. 5. In the proximal convoluted tubule, dense deposits were variable in size and shape with myeline figures. From the above experimental results it is thought that secondary biliary cirrhosis induces ultrastructural alterations in glomerular capillary wall and Ig A depositions in mesangial matrix, subepithelial and subendothelial areas.

o,p'-DDD가 흰쥐 뇌하수체 전엽의 ACTH분비의 활성도에 미치는 영향에 관한 면역조직화학적 연구

기근홍,최종범,임성철,박동수,이병래,전호종,서재홍,박규호 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

It has been well known that the toxicity of o,p'-DDD on the zona fasciculata in adrenal gland. The toxicity revealed adrenostatic effect on the cortisol biosynthesis by mitochodrial destruction. So, o,p'-DDD was used for unoperable adrenocortical carcinoma and its metastasis. The secretory activity of cortisol in adrenal cortex depended on ACTH secretion in the adenohypophysis. o,p'-DDD was dissolved in corn oil and administered orally for 28 days by 75 ㎎ / ㎏, 150㎎ / ㎏ and 300 ㎎ / ㎏ to investigate the adrenostatic effect on the cortisol biosynthesis of o,p'-DDD by assesment of ACTH secretory activity in anterior lobe of pituitary gland of rats. The results obtained were that the secretory activity of ACTH and the number of ACTH secretory cells were increased according to the dosage of o,p'-DDD, especially 300㎎ / ㎏ administrated group. Therefore, the suppression of cortisol biosynthesis of o,p'-DDD was confirmed by increased secretory activity of ACTH and the number of ACTH secretory cells.

불임여성 자궁내막에 관한 병리조직학적 연구

임성철,최종범,기근홍,전호종,서재홍 대한병리학회 1991 Journal of Pathology and Translational Medicine Vol.25 No.3

단핵구성 탐식세포의 분화에 관한 연구

서재흥,임성철,최종범,이길선,기근흉 최신의학사 1990 最新醫學 Vol.33 No.7