움직임 벡터의 공간적 국부 특성에 기반한 프레임간 내삽

성원락(Won Rak Sung),강응관(Eung Kwan Kang),최종수(Jong Soo Choi) 한국통신학회 1998 한국통신학회 학술대회논문집 Vol.1998 No.7

GnRH (Gonadotropin-Releasing Hormone)에 의한 자궁내막암 유래 세포주의 세포 증식 억제 기전에 있어서 Integrin, FAK (Focal Adhesion Kinase) 및 ERK (Extracellular Signal Regulated Kinase)의 역할

최종락,박동욱,최동순,민철기,Choi, Jong Rak,Park, Dong Wook,Choi, Dong Soon,Min, Churl K. 대한생식의학회 2006 Clinical and Experimental Reproductive Medicine Vol.33 No.2

Objective: To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II. Method: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [$^3H$] thymidine incorporation assay. Immunoblotting was utilized to detect proteins. Results: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin ${\beta}3$. Phosphorylation of FAK and ERK were induced by GnRH-I and -II. Conclusion: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation. 목 적: 본 연구를 통해 GnRH 의한 세포 분열의 억제는 integrin, FAK 빛 ERK를 통한 세포 내 신호전달 기전을 통하여 일어남을 규명하고자 하였다. 연구방법: 연구에 사용된 인간자궁내막암 세포주는 DMEM/F12 (10% FBS)의 조건에서 배양 하였다. GnRH-I과 -II는 실험 목적에 따라 100 nM 농도로 0, 5, 10, 15, 20, 30분간 또는 10 nM or 100 nM의 농도로 20분간 처리 하였다. 세포의 분열 정도는 [$^3H$] thymidine incorporation assay를 이용하여 정량적으로 측정 하였으며, Immunoblotting 방법을 이용하여 단백질의 발현을 확인 하였다. 결 과: GnRH-I과 -II 모두 HEC1A 세포의 세보분열을 억제하였으며 integrin ${\beta}3$의 발현을 증가 시켰다. GnRH-I과 -II를 처리 후 FAK 및 ERK의 안산화가 증가됨을 관찰할 수 있었다. 결 론: GnRH에 의한 세포분열의 억제는 integrin의 발현과 FAK 및 ERK의 인산화 과정을 통하여 일어남을 알 수 있었다.

재발된 전전뇌증

김종석(Jong Seok Kim),최종락(Jong Rak Choi),정철완(Chul Wan Jung),서경(Kyung Seo),김중열(Jung Yeol Kim),성연준(Youn Joon Sung) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7

Holoprosencephaly(HPE), a common developmental defect affecting the forebrain and cranioface, is etiologically heterogenous. Teratogen, chromosomal anomalies, genetic syndrome, or genetic disorder of non-syndromic HPE are usually accepted as etiology. But the severity of brain and craniofacial malformation are not associated with etiology. Individuals with microform of HPE, who usually have normal cognition and brain imaging, are at the risk of having children with HPE. Several studies on the basis of HPE gene have been performed, which shed valuable insight on normal brain development. As additional HPE genes are identified, more accurate recurrent risk counseling can be given. We experienced a case of recurrent HPE diagnosed by transabdominal ultrasound examinations at 22 weeks' gestation.

KSC-7 사용후핵연료 수송용기 핵임계해석

윤정현,최종락,곽은호,이흥영,정성환,Yoon, Jung-Hyun,Choi, Jong-Rak,Kwak, Eun-Ho,Lee, Heung-Young,Chung, Sung-Whan 대한방사선방어학회 1993 방사선방어학회지 Vol.18 No.2

본 연구에서는 사용후핵 연료를 안전하게 수송할 수 있는 수송용기의 여러 가지 설계 항목중에 수송용기 내부에 장전한 핵연료에 의한 핵임계반응을 방지하기 위한 핵임계해석을 수행하였다. 핵임계 해석에 사용한 HANSEN-ROACH-KENO-Va 전산시스템에 대한 검증계산을 수행하였고 수송용기의 핵임계측면에서의 안전성을 확보하기 위해 가능한 보수적인 가정을 하여 어떠한 경우에도 수송용기에 장전된 핵연료가 임계상태에 도달하지 않도록 수송용기 내부의 구조 및 적절한 핵임계 방지제를 선택하였고 정상수송 및 가상사고 조건 등에 대한 해석을 수행하였다. 그 결과 KSC-7 수송용기 의 설계조건을 만족하고 핵임계측면에서의 안전성을 보장할 수 있는 재료 및 구조에 대한 결론을 해석적으로 도출하였다. The criticality of the shipping cask(KSC-7) for transportion of 7PWR spent fuel assemblies has been calculated and analysised on the basis of neutron transport theory. For criticality analysis, effects of the rod pitches, the fixed neutron absorbers(borated sus+boral) were considered. The effective multiplication factor has been calculated by KENO-Va, Mote Carlo method computer code, with the HANSEN-ROACH 16 group cross section set, which was made for personal computer system. The criticality for the KSC-7 cask was calculated in terms of the fresh fuel which was conservative for the aspects of nuclear critility. From the results of criticality analysis, the calculated Keff is proved to be lower than subcritical limit during normal transportation and under hypothetical accident condition. The maximum calculated criticalities of the KSC-7 were lower the safety criticality limit 1.0 recommended by US 10CFR71 both under normal and hypothetical accident condition. Also, to verify the KSC-7 criticality calculation results by using KENO-Va, it was carried out benchmark calculation with experimental data of B & W(Bobcock and Wilcox) company. From the 3s series of calculation of the KSC-7 cask and benchmark calculation, the cask was safely designed in nuclear criticality, respectively.

중합연쇄반응 제한효소 단편 장다형을 이용한 듀센형 근이영양증 산전 진단

차동현(Dong Hyun Cha),이국(Kook Lee),최종락(Jong Rak Choi),송경순(Kyung Soon Song) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

목적 : 듀센형 근이영양증(이하 DMD)은 X-염색체 열성으로 유전되며, 디스트로핀 유전자의 변이로부터 발생한다. DMD의 산전진단에 있어서 중합 연쇄반응 제한효소 단편 장다형의 효율성을 보고자 한다. 연구 방법 : DMD 가계원의 혈액과 태아의 양막세포로부터 DNA를 추출하였고, 5개의 RFLP probe를 이용하여 중합 연쇄반응 제한효소 단편 장다형을 시행하였다. 결과 : 가계 A는 다양한 엑손 결실(6, 8, 12, 13, 17)이 관찰된 가계로서 산모는 pERT84에 이형접합을 보였고, 태아는 DMD 환자와 같은 대립형질을 나타내었고, 유전자형(genotype) 검사상 역시 같은 엑손 결실을 나타내어, 18주에 임신 종결하였다. B 가계의 산모는 pERT84와 pERT87-15에서 이형접합을 보였고, 보인자로 생각되며, 태아는 DMD 환자와 다른 대립형질을 가져 재조합이 없다면 정상으로 생각되었다. C 가계의 산모는 pERT84에서 이형접합을 보였으며, 태아는 역시 환자와 다른 대립형질을 가져 정상으로 생각되었다. 두 가계의 태아는 현재 분만되었고, 정상적으로 생활하고 있으며 추적 관찰 중이다. 결론 : DMD의 예방에는 산전진단이 무엇보다도 중요하다. 중합 연쇄반응 제한효소 단편 장다형은 DMD를 진단하는데 있어서 매우 신속하고 정확한 검사법으로서, 특히 임신 초에 진단이 가능하여 산전진단에 유용하리라 생각된다. Objective : Duchenne muscular dystrophy(DMD) is a X-linked recessive disease and results from mutation in the dystrophin gene. In this study, we evaluate the efficacy of polymerase chain reaction-restriction fragment length polymorphism in prenatal genetic diagnosis of DMD.Methods : DNA was isolated from DMD family's blood and fetal amniocyte and used to perform PCR-RFLP. In DMD family(3 cases), linkage analysis was tried with 5 RFLP probes.Results : DMDs of the family A had mutiple exon deletions(6, 8, 12, 13, 17). The mother was a heterozygote of pERT84;MaeIII. The male fetus had a same allele and also same exon deletions with the affected males. The pregnancy was terminated at IUP 18 gestational weeks. Pregnant woman of the family B was heterozygote of both pERT84;MaeIII and pERT87-15;BamHI, and pregnant woman of the family C was of pERT84;MaeIII. The both male fetuses , as compared with the affected male of each family, had a different allele. Thus, the fetuses were probably not affected with a confidence level of 95%. Conclusion : Prenatal diagnosis in prevention of DMD is most important. PCR-RFLP analysis in DMD family is rapid and useful diagnostic tool.

에세폰과 ABA 處理가 葡萄의 糖 및 酸含量에 미치는 影響

金善圭(Kim Seon-Kyu),崔東龍(Choi Dong-Yong),朴鍾天(Park Jong-Cheon),吳鎭煥(Oh Jin-Hwan),崔宗洛(Choi Jong-Rak) 한국원예학회 1998 Horticulture, Environment, and Biotechnology Vol.39 No.5

증례 : 혈액종양 ; AML1-ETO 양성인 양표현형 급성 백혈병의 1예

서주희 ( Ju Hee Seo ),이혜원 ( Hye Won Lee ),임주은 ( Ju Eun Lim ),정주원 ( Joo Won Chung ),최종락 ( Jong Rak Choi ),양우익 ( Woo Ick Yang ),민유홍 ( Yoo Hong Min ) 대한내과학회 2009 대한내과학회지 Vol.76 No.5

BAL은 일반적으로 기타 급성 백혈병보다 예후가 나쁘다고 알려져 있으나, 그 치료방침이 아직 확립되어 있지 않다. AML1-ETO 유전자 재배열은 BAL에서도 좋은 예후인자 중 하나로 제시된 적이 있으며 본 증례에서도 1차례의 관해 유도요법 후 완전관해를 보였고, t(8:21) 즉, AML1-ETO 정량검사상 전사 정도가 현저히 감소함을 경험하였다. 특히, AML에 준한 치료만으로 성공적 관해가 유도되었으며, 이는 BAL에 있어서도 AML1-ETO 종양단백이 병태생리에 주 역할을 할 것임을 짐작할 수 있다. AML에서와 마찬가지로 AML1-ETO 유전자 재배열은 향후 BAL 환자의 치료 효과와 예후를 알 수 있는 지표로 사용될 수 있을 것이다. Biphenotypic acute leukemia (BAL) is a subtype of acute leukemia that expresses two different immunophenotypic lineages, most commonly myeloid and either B- or T-lymphoid lineages. This entity has been defined by a scoring system proposed by the European Group for the Immunological Characterization of Leukemias (EGIL). The prognosis of BAL is regarded as being worse than either acute lymphoid or myeloid leukemia that does not show lineage ambiguity. However, a treatment strategy for BAL has not yet been established. We experienced a case of BAL with the t(8;21) translocation, a favorable cytogenetic rearrangement in acute myeloid leukemia (AML). The patient was successfully treated with cytarabine and anthracycline for induction and consolidation. The quantitative value of the AML1-ETO gene decreased after achieving complete hematologic remission. Thus, the AML1-ETO gene rearrangement in BAL may be associated with an acceptable response to the treatment strategy for AML. (Korean J Med 76:617-621, 2009)

KCNT1 돌연변이가 확인된 영아 이동성 부분 발작 뇌전증 환아에서의 Quinidine 치료를 시도한 영아 1예

지나리(Nalee Jee),고아라(Ara Ko),김세희(Se Hee Kim),이준수(Joon Soo Lee),김흥동(Heung Dong Kim),이승태(Seung-Tae Lee),최종락(Jong Rak Choi),강훈철(Hoon Chul Kang) 대한소아신경학회 2017 대한소아신경학회지 Vol.25 No.3

Epilepsy of infancy with migrating focal seizure (MFEI) is an early-onset epileptic encephalopathy characterized by randomly migrating focal seizures and psychomotor deterioration. It is associated with mutations in a variety of genes, with potassium sodium-activated channel subfamily T member 1 (KCNT1) being an example. Previously reported KCNT1 mutations in MFEI are gain-of-function mutations. Therefore, quinidine therapy targeted at reduction of pathologically increased KCNT1 channel-mediated potassium conductance has been proposed as a target treatment for MEFI with KCNT1 mutation. The authors report a case involving a patient with MFEI and a missense mutation in KCNT1 (c.7129G>A; p.Phe346Leu) treated with quinidine therapy. Seizure activity was poorly responsive to quinidine.

모체의 말초 혈액에 존재하는 태아 세포 분리에 의한 새로운 기형아 진단 - Microdissection 에 의한 CGH 기법의 사용

양영호(Young Ho Yang),김성훈(Sung Hoon Kim),김세광(Sei Kwang Kim),박용원(Yong Won Park),조재성(Jae Sung Cho),김인규(In Kyu Kim),최종락(Jong Rak Choi),김미순(Mi Soon Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.2

목적 : 모체의 말초 혈액에서 분리된 태아의 유핵 적혈구에 CGH기법을 이용하여 태아 염색체 이상을 진단함에 있어 임상적 유용성을 알아보고자 한다. 방법 : 임산부의 말초혈액을 채취하여 heparin 처리한 후 triple density gradient 원심분리, CD45와 CD71을 이용한 MACS의 방법을 거쳐 태아의 유핵 적혈구를 분리한다. 얻어진 태아의 유핵 적혈구들을 microdissection하여 분리, DOP (degenerate oligonucleotide primed) - PCR (polymerase chain reaction)로 증폭시킨 후, nick translation과 CGH를 시행한다. 결과 : 모든 예에서 모체의 말초 혈액으로부터 태아의 유핵 적혈구가 분리 가능하였으며, 이는 Kleih uer- Betke 염색법에 의하여 증명되었다. 얻어진 태아 유핵 적혈구들에 microdissection, DOP-PCR, Nick translation 시행 후의 DNA의 크기는 교잡에 적합하였으며, CGH를 시행하여 정상 여자 태아와 trisomy 21 남자 태아를 진단할 수 있었다. 결론 : 모체의 말초 혈액에서 분리된 태아 유핵 적혈구를 이용한 CGH 분석을 통한 태아 염색체 이배수성의 산전 유전 진단법은 정확하고 빠르며, 비침습적인 방법으로써 임상적으로 유용하다. 향후 더 많은 예의 연구가 되어진다면, 이러한 방법은 산전 유전 진단에 있어 더욱 유용하게 사용될 것으로 사료되어 진다. Objective: The objective of this study was to determine the clinical use of CGH (comparative genomic hybridization) for detection of fetal aneuploidy from fetal cells (nucleated red blood cells, nRBCs) isolated Methods: Maternal peripheral venous blood sample was collected and treated by heparin. Triple density gradient centrifugation, and MACS (magnetic activated cell sorting) using CD45 and CD 71 were used to isolated the fetal nRBCs. With microdissection, DOP (degenerate oligonucleotide primed)-PCR (polymerase chain reaction), and nick translation, CGH was performed. Results: Fetal nRBCs were successfully isolated from maternal peripheral blood. After microdissection of fetal nRBCs, DOP-PCR. and nick translation, DNA size was suitable for hybridization. In CGH analysis, we can confirm normal female and trisomy 21 male fetus. Conclusion: Prenatal diagnosis from fetal cells in maternal peripheral blood by comparative genomic hybridization shows clinical promise in terms of speed, accuracy, and non-invasiveness. To enable widespread use of this method, further studies involving many cases are warrented.

관동맥 질환에서 혈청 Troponin T 의 임상적 의의

이병권(Byoung Kwon Lee),권혁문(Hyuck Moon Kwon),박현영(Hyun Young Park),박광주(Kwang Joo Park),김현승(Hyun Seung Kim),최종락(Jong Rak Choi),송경순(Kyung Soon Song) 대한내과학회 1996 대한내과학회지 Vol.50 No.1

N/A Objectives: Although the diagnosis of coronary heart disease is usually straight forward, conventional diagnostic tests, such as CK, CK-MR, LDH, and AST have several drawbacks. The diagnostic efficiency of these conventional thests is disappointingly low, especially in minimal myocardial damage. l herefore, there has be necessity of more sensitive and specific diagnostic thest. Recently, cardiac TnT (TnT) was introduced with better sensitivity, specificity, and more wide diagnostic window. So we analyzed distribution of TnT in control, release kinetics, diagnositic significance of TnT in coronary heart disease including unstable angina and acute myocardial infarction, and efficiency of TnT as a marker of reperfusion in acute myocardial infarction. Methods: We tested cardiac TnT in 40 normal healthy subjects and 25 extracardiac traumatic patients as control group. We also tested cardiac TnT in 34 patients with acute myocardial infarction and 27 patients with unstable angina. We evaluated the distribution and serial change of cardiac TnT with other cardiac enxzymes after chest pain onset, the discriminant power of Tnl in discriminating of reperfusion after thrombolytic therapy in patients with acute myocardial infarction with conventional coronary angiography and the correlation between release kinetics of TnT and left ventricular ejection fraction with echocaroliography. Results: 1) In control group, all 65 cases serum TnT value were less than 0.2㎍/L. And its specificity (100%) and sensitivity in diagnosis of coronary heart disease including untable angina(acute myocardial infarction: 100%, unstable angina 70.4%) was better than those of other cardiac enzymes, such as CK, CK-MB. 2) In patient group, the release pattern of cardiac TnT was in bimodal curve pattern, while the pattern of other cardiac enzymes was in unimodal pattern. 3) In acute myocardial infarction, the first peak value of cardiac TnT appeared siginificantly earlier (9.53±2.36 hr in stable-reperfused group, 13.40±1.92 hr in non-reperfused group of acute MI than that of caridac enzymes; CK(17.65±6.48 hr in stable-reperfused group 27.53±10.26 hr in non-reperfused group), and CK-MH (16.84±7.93hr in stable-reperfused group, 24.33±10.36 hr in non-reperfused group of acute MI)(p<0.01). And troponin T was continuously increased above the cut-off value during the test period, so the diagnostic window of TnT was longer than other cardiac enzyme. 4) In acute myocardial infarction, cardiac TnT showed better discriminant power above 80% for reperfusion than those of CK(67.65%) and CK-MB (58.82%) In stable reperfused group of acute MI, cardiac TnT showed significant correlation with left ventri- cular ejection fraction(absolute value of r >0.6, p< 0.001). Conclusion: Cardiac troponin T was a good diagnostic marker for coronary heart disease including unstable angina and acute myocardial infarction with better sensitivity and specificity, for discriminating of reperfusion after thrombolytic therapy, and for the prognosis of remnant left ventricular global systolic function