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두릅 추출물의 기능성 원료 표준화를 위한 HPLC 분석법 검증
안은미,최송암,최지영,Ahn, Eun-Mi,Choi, Song-Am,Choi, Ji-Young 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.6
개별인정형 건강기능식품 기능성 원료로 개발한 두릅추출물의 표준화를 위해 항고혈압활성 효과를 가지고 있는 기능성분 HE를 지표성분으로 설정하고, HPLC/UV 방법을 이용하여 기능성분 분석법을 확립하며 그에 따른 유효성 검증을 실시하고자 하였다. 유효성 검증 결과, 본 시험법에서 표준용액의 피크유지시간과 두릅추출물의 피크유지시간이 일치하였다. 검량선의 결정 계수(R2)는 0.9999 이상의 높은 직선성을 보여 분석에 적합함을 알 수 있었으며, 검출 한계는 $12.0{\mu}g/mL$, 정량한계는 $36.5{\mu}g/mL$로 두릅 추출물 중의 HE 함량분석을 위한 충분한 한계 수준으로 확인되었다. 정확성에서 $98.8{\pm}0.865-104.7{\pm}2.536%$로 높은 수준의 HE 회수율을 보였으며, 평균 분석오차는 0.013%로서 기준인 10% 이내를 만족하였다. 또한 반복성에서는 평균 $101.5{\pm}0.4%$로 RSD 0.4%를 나타내었고, 실험실내 정밀성에서 RSD 0.2%, 완건성에서 RSD 0.2%를 나타내어 모두 적합함을 확인하였다. 한편, 제주산 두릅추출물 중에는 기능성 지표성분인 HE가 4.88% 함유되어 있는 것으로 확인되었다. 이상의 실험 결과, 두릅추출물의 항고혈압활성 기능성분인 HE의 HPLC/UV 분석방법은 특이성, 직선성, 정확성, 정밀성, 반복성, 완건성 등의 모든 항목에서 validation 기준에 적합한 시험 방법으로 확인되었다. Aralia elata Seemann (AE) has long been used as a folk medicine for the treatment of various diseases including diabetes mellitus, anti-arthritic, and anti-gastric ulcer agent in Korea, Japan, and China. This study was performed to establish a simple and reliable HPLC/UV analytical method for determination of most active anti-hypertensive compound, a 3-O-${\alpha}$-L-rhamnopyranosyl($1{\rightarrow}$2)-${\alpha}$-L-arabinopyranosyl hederagenin 28-O-${\beta}$-D-xylopyranosyl($1{\rightarrow}6$)-${\beta}$-D-glucopyranosylester (HE) for the standardization of the shoot extract of AE as a health functional food ingredient. The quantitative analytical method of HE was optimized by HPLC analysis using reverse-phase C18 column at $40^{\circ}C$ with $H_2O$ and acetonitrile (70:30, v/v) as an isocratic mobile phase at a flow rate of 1.0 mL/min and detection wavelength of UV 205 nm. This HPLC/UV analytical method showed good specificity and high linearity in the tested range of 0.03125-2.0mg/ml with excellent coefficient of determination ($R^2$) of 0.9999. The limit of detection and limit of quantification were $12.0{\mu}g/mL$ and $36.5{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 0.2% and 0.1%, respectively. These results indicate that the established HPLC/UV analytical method is very simple, specific, precise, accurate, and reproducible and thus can be useful for the quantitative analysis of HE as a functional anti-hypertensive compound in AE extract.
이승용(Seung Yong Lee),정연복(Youn Bok Chung),한건(Kun Han),최송암(Song Am Choi) 대한약학회 1994 약학회지 Vol.38 No.1
From phase solubility studies bile acids and bile salts were found to form stable inclusion complexes with beta-cyclodextrin in aqueous solution. Stability constant of bile acids were larger than that of bile salts. Phase solubility diagrams of most bile acids showed Higuchi''s AL type but lithocholic acid showed Bs type. Not only the solubility of bile acids but also that of beta-cyclodextrin increased, especially in cases of cholic acid and ursodeoxycholic acid. Solubility increase of bile acids from their beta-cyclodextrin inclusion complex followed the order : cholic acid>ursodeoxycholic acid>chenodeoxycholic acid>deoxycholic acid>lithocholic acid. It seems that solubility of inclusion complexes was directly related with the hydrophilicity of bile acids.
데커시놀추출물의 경구투여후 흰쥐에 있어서의 약물속도론적 연구
김지혜,최송암,김동출 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3
The objective of this study is to investigate the pharmacokinetic parameters of decursinol following oral administration in Sprague-Dawley rats. The plasm concentration of decursinol was determined by LC/MS with APCL positive mode. The m/z value of decursinol was observed at 247. Folowing oral administration of decursinol extract, the apparent clearance was 5.3±2.7 ml/hr/rat, the absorption half life was 2.5±0.41 hr, the elimination half life was 3.05±1.57 hr and the apparent volume of distribution was 21±12 ml/rat. The LC/MS method was successfully applied to the phar-macokinetic study of decursinol.