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TGF- ${\beta}$ 첨가가 한우 난포란의 체외성숙에 미치는 영향
최선호,이혜현,연성흠,한만희,김현종,조상래,우제석,백광수,류일선,손동수,Choi, Sun-Ho,Lee, Hye-Hyun,Yeon, Seong-Heum,Han, Man-Hye,Kim, Hyun-Jong,Cho, Sang-Rae,Woo, Jae-Seok,Baek, Kwang-Soo,Ryu, Il-Sun,Son, Dong-Soo 한국발생생물학회 2004 발생과 생식 Vol.8 No.2
소 체외성숙시 난포란에 영향을 주는 과립막세포와 난자의 핵성숙을 촉진하는 물질이 확인되지 않은 혈청내의 물질 뿐만 아니라 호르몬이나 생리활성인자 등에 의해 촉진됨이 밝혀졌다. 이에 따라 체외성숙 및 체외발달에 사용되는 배양액의 조성도 복합배양액에서 단순배양액으로 전환을 시도하고 있으며, 체내의 조건에 보다 더 접근하고자 하는 시도들이 수행되고있다. 본 연구는 한우 난포란의 체외성숙율 및 체외수정 후 배발달율을 향상시키기 위하여 TGF-${\beta}$의 첨가가 미치는 영향에 대하여 조사하였다. 한우 난포란의 체외성숙시 TGF-${\beta}$ 0.1, 1. 10ng/ml를 첨가하였을 때 24시간에 MetaphaseⅡ 도달율은 95.8%, 95.8%, 100%를 나타내었으며, 농도간의 유의적인 차이는 없었다. TGF-${\beta}$로 체외성숙된 난포란을 체외수정 후 TGF-${\beta}$가 체외성숙 배양액을 동일하게 체외발달을 유도하였으나, 0~0.8%의 배반포율을 보였으며, TGF-${\beta}$로 체외성숙된 난포란을 체외수정 후 TCM 199_10% FBS, 0.8% BSA, 0.1% PVA에 배양한 결과 12.4%, 12.8%, 8.5%의 배반포 발달율을 보였고, 무혈청 배양액인 IVMD, IVD에 배양한 결과 38.4%, 34.8%의 배반포 발달율을 보였으며, 유의적인 차이를 나타냈다(P<0.005). 결론적으로 TGF-${\beta}$는 한우 난포란의 체외성숙시 상당히 유용한 물질이나, 체외발달에는 만족할 수 없어, 이외의 생리활성 인자들간의 상호관계에 대하여 더 많은 요구가 요구된다. It is well known that unidentified factors in sera, hormones and growth factors promote the proliferation of granulosa cells and nuclear maturation of bovine COCs in vitro. Attemps had been developed the simple composition of culture media and similar system to in vivo conditions has been applied. In the present study, we investigated the effect of TGF-${\beta}$ on in vitro maturation and in vitro development of Hanwoo COCs. When the COCs were matured in TCM 199 containing 0.1, 1 or 10 ng/ml TGF-${\beta}$ for 24 hrs, metaphaseⅡ of COCs were obtained 95.8%, 100% of matured COCs, respectively and there were no differences among the concentrations of TGF-${\beta}$. Matured COCs with TGF-${\beta}$ cultured in maturation medium after in vitro fertilization, developmental rate to blastocyst were 0~0.8%. Matured COCs with TGF-${\beta}$ were cultured in TCM 199+10% FBS, 0.8% BSA, 0.1% PVA, blastocyst formation were showed in 12.4%, 12.8%, 8.5% of those and cultured in IVMD or IVD without serum were 38.4%, 34.8%, respectively. There were significant differences among the media (P<0.05). TGF-${\beta}$ is available for i vitro maturation of bovine COCs, but further investigation would be need for finding the synergistic autocrine/paracrine fashion of other growth factors in early bovine development.
최선호,Choi, Sun-Ho 대한기계학회 1977 대한기계학회논문집 Vol.1 No.2
It is well known fact that there is a theoretical analogous relation ship in terms of differential equation between the stress distribution of a bar under pure torsional moment and the deflection of a membrane subjected to a uniform pressure. This relationship has been applied to the experimental determination of torsional stresses with troublesome and elaborative measuring procedure since Griffith's invention of the soap-film apparatus. In this paper, the soap-film was replaced by a highly polished steel plate and whole visualable deflectional contour-line-map was able to obtain by using Michelson Interferometer with Ne-He Gas Laser lignt source enabling to determine the stress distribution in the vicnity of the symmetrical inner-boundary in a section. Experimental results were satisfactory and the margin of error in the measurement was lower than 1%.
5.25GHz 무선 LAN을 위한 T-Slot Weathercock-Shaped 마이크로스트립 패치 안테나 설계 및 제작
최선호,정계택,이화춘,곽경섭,Choi Sun-Ho,Jeong Gyey-Teak,Lee Hwa-Choon,Kwak Kyung-Sup 한국통신학회 2005 韓國通信學會論文誌 Vol.30 No.12A
본 논문에서는 5.15-5.35GHz Wireless LAN 대역의 사용을 위한 Weathercock-Shaped의 마이크로스트립 패치 안테나에 대하여 설계 및 제작하였다. 이동성을 위해 소형화하였고 Weathercock패치 모양에 T-Slot을 삽입하여. 적절한 대역폭을 얻고자 하였다. 시뮬레이션 설계는 Weathercock 모양과 T-Slot의 길이, 폭 그리고 급전위치를 최적화하여 제작 및 측정하였고 단일 양면 기판만을 사용해서 제작하였다. 실제 제작된 안테나의 측정결과는 Voltage Standing Wave Ratio (VSWR) < 2에서 $7.62\%$의 대역폭과 5.25${\~}$6.70dBi의 Gain을 얻었으며 3-dB 빔폭은 Azimuth와 Elevation이 각각 $80.32^{\circ}$와 $83.88^{\circ}$이 측정되었다. In this paper, a weathercock-shaped microstrip patch antenna for application in 5.25GHz band wireless LAN is designed and fabricated. To obtain sufficient bandwidth in VSWR<2, the T-slot is inserted on the patch, the coaxial probe source is used. The measured result of fabricated antenna obtained 350MHz or about $7.62\%$ bandwidth in VSWR<2 referenced to the center frequency, the gain of 5.25${\~}$6.70dBi. The experimental 3-dB beam width is shown to be broad across the pass band in azimuth and elevation at $80.32^{\circ}$ and $83.88^{\circ}$, in several.
공동주택 주방 배기 시스템 구성 요소별 정압손실 특성에 따른 주방환기 성능에 관한 실험적 연구
최선호(Choi, Sun-Ho),이건태(Lee, Geon-Tae) 대한건축학회 2012 대한건축학회논문집 Vol.28 No.3
The purpose of this study was to analyze the static pressure loss of exhaust fan affected by each component of kitchen ventilation system which is consisted of kitchen hood, filter, flexible or rigid duct and exhaust cap. Different kinds of each part of the kitchen ventilation system were chosen respectively among the material and components mainly installed in apartment housing. The static pressure was measured by test chamber that air flow rate is able to be controlled by 4 nozzles, inverter fan and controller. As a result, a high static pressure fan must be used in order to effectively prevent the spread of odor and air pollutant occurred in the kitchen of high air-tightness housing kitchen ventilation system because static pressure was higher than expected. If not, each part of kitchen ventilation system which static pressure loss is low will be needed.