한국 재래식 간장덧 발효시 대두 자숙 폐액 첨가가 젖산발효 촉진에 미치는 영향

최광수,최청,임무혁,최종동,정현채,김영호,이춘우 ( Kwang Soo Choi,Cheong Choi,Moo Hyeog Im,Jong Dong Choi,Hyun Chae Chung,Young Ho Kim,Choon Woo Lee ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3

In order to evaluate the effects of addition of soybean boiling waste liquor (SBWL) and sugar and inoculation of the lactic acid bacteria and yeast starter culture in Korean traditional kanjang mash, three types of kanjang were prepared in a clay pot of 100 l volume and compared the characteristics of lactic acid fermentation. The mashing compositions of the types of kanjang were as follows: (1) control treatment mash was prepared with meju : 20% salt solution (1:4) and SBWL, (2) kanjang mash with 3.5% added sugar to the control type mash and (3) kanjang mash with 3.5% added sugar and inoculation of the lactic acid bacteria and yeast starter culture 35 days after mashing to the control type mash. (1), (2) and (3) of kanjang mash were found to be effective in increasing the lactic acid content and improving the organoleptic characteristics of kanjang. But the effect of yeast starter culture was not clear because osmophilic yeasts were inhibited by metabolite(acetic acid) produced by lactic acid bacteria. The lactic acid content of (1), (2) and (3) kanjang was 2.05, 2.38 and 2.91% respectively in 90 day-matured kanjang.

육용 종계의 주요 경제형질에 대한 유전변이 추정에 관한 연구 1 . 양면교잡에 의한 육용 종계 체중의 유전변이 추정

최광수,오봉국,여정수 ( Kwang Soo Choi,Bong Kug Ohh,Jung Sou Yeo ) 한국축산학회 1980 한국축산학회지 Vol.22 No.6

The study was conducted to obtain some genetic information for developing superior broiler breeder stock. Genetic variations were estimated with 997 progenies of $quot;4 × 4$quot; dialled crosses. The progenies were raised at the Livestock Experimental Farm, College of Agriculture, Seoul National University from March, 1974 to June, 1975. Body weights at 8, 24 and 64 weeks of age were investigated and analyzed into additive gene effects, dominance effects and heritabilities with Hayman`s model. The estimates of these effects were made with $quot;4 × 4$quot; dialled tables of three White Plymouth lock lines (RF, SF, TF) and a line of White Cornish (TM), and again with $quot;3 × 3$quot; diallel tables of three White Plymouth Rock lines in order to compare the effects from breed-crosses and line-crosses of the same breed. In body weights at the ages of 8 and 24 weeks, the genetic variation due to additive among parental lines showed significant differences in both cases of breed-crosses and line-crosses. However, dominance effects appeared in case of breed-crosses, and the effects of overall dominance and line dominance slowed significant differences. No significant differences were observed in genetic variation on body weight at the age of 64 weeks. Heritability estimates for the body weights at the age of 8, 24 and 64 weeks were 0.77, 0.74 and 0.14 in the White Plymouth Rock ; and 0.49, 0.35 and 0.08 in the crossbred of White Plymouth Flock and White Cornish, respectively.

손목 자세와 외부 부하에 따른 손목 불편도 모델링

최광수,박재규,정의승,최재호,Choi, Kwang-Soo,Park, Jae-Kyu,Jung, Eui-S.,Choe, Jae-Ho 대한인간공학회 2005 大韓人間工學會誌 Vol.24 No.3

The objectives of this study are to analyze representative wrist postures while using hand tools and parts at general assembly processes, to evaluate perceived discomfort on the wrist when external loads are present, and to suggest an evaluation and prediction model of perceived discomfort. Sixteen subjects participated in an experiment to appraise perceived discomfort. Three types of the wrist postures with five levels of non-neutralities were analyzed when five levels of external load were applied to each posture. The ANOVA results showed that the perceived discomfort of wrist postures was significantly affected by both the wrist posture and external load (p$<$0.001). It was also shown that some of the interactions between external loads and the wrist postures(Flexion/$Extension^*$Load, Flexion/$Extension^*$supination/pronation, ulnar/radial $deviation^*$supination/pronation) were significant(p$<$0.001). The result implies that a new posture classification scheme for workload assessment methods may be needed to reflect such effects of external load and wrist posture. A regression model of perceived discomfort was developed with respect to wrist posture and external load from the experimental data. A subsequent experiment revealed that the correlation coefficient between the predicted values of perceived discomfort from the model and the actual values obtained from the experiment was about 0.98. It is expected that the results help to properly estimate the body stress resulting from worker's postures and external loads and can be used as a valuable design guideline to analyze potential hazard of musculoskeletal diseases in industry.

한국 재래 간장에서 분리한 Scopulariopsis brevicaulis가 생성하는 ${\alpha}-galactosidase$의 특성 및 작용양상

최광수,이선호,홍승표,이희덕,배두경,최청,Choi, Kwang-Soo,Lee, Seon-Ho,Hong, Seung-Pyo,Lee, Hee-Duck,Bae, Du-Kyung,Choi, Cheong 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.7

한국 재래 간장으로부터 분리한 Scopulariopsis brevicaulis가 생성하는 ${\alpha}-galactosidase$의 최적 생산조건은 tryptone 1.5%, $NH_4NO_3$ 0.2%, raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$에서 3일간 배양 했을 때였다. ${\alpha}-galactosidase$의 최적 pH는 7.0, 최적온도는 $27^{\circ}C$였으며, $pH\;6.0{\sim}8.0$범위와 $40^{\circ}C$이하에서 안정하였다. $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$ 등의 금속이온 및 p-chloromercuribenzoic acid, Iodine에 의해 강하게 저해되어 본 효소의 catalytic부위에 -SH기가 있음이 추정되었다. Km값은 1.9 mM, Vmax값은 $9.66{\times}10^2\;{\mu}M/min$이었다. 배양기간에 따른 raffinose의 분해양상을 high performance liquid chromatography로 살펴본 결과 배양초기에 raffinose가 분해되어 sucrose, glucose, fructose가 관찰되었다. 배양기간이 경과함에 따라 raffinose가 감소하였고 sucrose, glucose, fructose도 소실됨을 확인하였다. The optimum culture condition of Scopulariopsis brevicaulis for the production of ${\alpha}$-galactosidase was as follows: Tryptone 1.5%, $NH_4NO_3$ 0.2%, Raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$. The optimum pH and temperature for the enzyme activity of ${\alpha}$-galactosidase producing Scopulariopsis brevicaulis were pH 7.0 and $27^{\circ}C$, respectively. The enzyme was relatively stable at $pH\;6.0{\sim}8.0$ and at temperature below $40^{\circ}C$. The activity of the enzyme was inhibited by $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$, p-chloromercuribenzoic acid and Iodine. These results would indicate the presence of -SH groups in the catalytic site of the enzyme. Km value was 1.9 mM for $p-nitrophenyl-{\alpha}-D-galactopyranoside$ and Vmax value was $9.66{\times}10^2\;{\mu}M/min$. Sugar constituents of culture broth were identified by HPLC that the enzyme liberated sucrose, glucose and fructose from raffinose and raffinose was significantly decreased.

테트라싸이클린 함유 차폐막을 이용한 골조직 유도 재생에 관한 연구

최광수,김탁,양대승,김은철,유형근,신형식,Choi, Kwang-Soo,Kim, Tak,Yang, Dae-Seung,Kim, Eun-Cheol,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.2

Application of membranes for guided tissue regeneration(GTR) have been confined to the subgingival barrier functions; however, many studies have provided evidence that some drugs, including tetracycline, initially can promote the growth of periodontal ligament or alveolar bone in peridontal therapy. Osseous regeneration in periodontal defects is increased by local administration of tetracycline due to its anti-collagenolytic effect, which enhances bone-forming ability via osteoblast cell chemotaxis and reduced bone resorption. The aim of this study was to evaluate effects of tetracycline loaded poly-L-lactide(PLLA) barrier membranes for guided bone regenerative potential. Tetracycline was incorporated into the PLLA membrane with the ratio 10% to PLLA by weight. Ability to guided bone regeneration of the membranes were tested by measuring new bone in the tibial defects($7{\times}10{\times}5\;mm^3$) of the beagle dog for 4,5, and 6 weeks. In control, drug-unloaded PLLA membranes were used in same size of defect. In histologic finding of the defect area, a few inflammatory cells were observed in both groups. These membrane were not perforated by connective tissue and maintained their mechanical integrity for the barrier function for 4-6 weeks. New bone formation was greater in defects covered by tetracycline-loaded membrane than in defects covered by drug- unloaded membranes. In bone regeneration guiding potential test, tetracycline-loaded membrane was more effective than drug- unloaded membranes(p<0.05). These results suggest that tetracycline-loaded PLLA membranes potentially enhance guided bone regenerative efficacy and might be a useful barrier for GTR in periodontal treatment.

충전층 반응기내에서 고정된 Pseudomonas aeruginosa에 의한 Cd²⁺의 제거

최광수,김철경,Choi, Kwang Soo,Kim, Chul Kyung 한국청정기술학회 2002 청정기술 Vol.8 No.4

본 연구에서는 수직 원통형 충전반응기(packed-bed column reactor)내에 균체가 고정화된 Ca-alginate bead를 넣어 카드뮴이온을 제거하는데 있어 반응기내로 유입되는 유속, 초기농도 등에서 카드뮴 이온($Cd^{2+}$) 제거하는데 최적 조건을 찾고자 하였다. 사용된 균주(Pseudomonas aeruginosa ATCC 27853)는 한국과학기술원 유전공학센터 유전자은행(KCTC)으로부터 구입하였고, Bead로 사용된 Ca-alginate bead는 Sodium Alginate에 균체와 $CaCl_2$를 섞어 제조한 것을 사용하였다. 실험 결과 동일조건일 때 유속이 30 mL/hr > 45 mL/hr > 60 mL/hr의 순서로 제거율이 좋았고 유입속도의 변화에도 불구하고 각 유속에서 50 ppm > 100 ppm > 200 ppm > 300 ppm의 순으로 좋은 제거율을 보였다. The effects of initial cadmium ion concentrations (50, 100, 200, 300ppm), and feeding velocities (30, 45, 60mL/hr) on the removal ratio of cadmium ion by Pseudomonas aeruginosa ATCC 27853 immobilized in Ca-alginate gel beads in a packed-bed column reactor were investigated at operating temperature $37^{\circ}C$. The removal ratio of cadmium ion with variable initial concentration was decreased in the following order : 50ppm > 100ppm > 200ppm > 300ppm. The optimum removal conditions of cadmium ion by Pseudomonas aeruginosa ATCC 27853 were initial concentration 50ppm, feeding velocity 30mL/hr.

豆腐製造時 大豆의 水浸時間에 따른 水溶性糖類 및 豆腐收率의 變化

최광수(Kwang Soo Choi),김순희(Soon Hee Kim) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.4

두부 제조시 대두 내에 함유된 장내 가스 생성성분인 raffinose등의 수용성 소당류가 두부로 적게 이행되는 방법을 구명하기 위하여 대두의 수침 시간을 5, 10, 24시간으로 연장시켜서 제조된 두유, 두부 및 순물 내의 이들 당류의 함량 및 두부수율의 변화를 조사하여 다음과 같은 결과를 얻었다.<br/> 1. 대두의 수침 시간을 5, 10, 24시간으로 연장시킴에 따라 두유내의 포도당 총량은 대두에 함유되었던 포도량 총량에 비하여 225, 425 및 431%씩 각각 현저히 증가하여 나타났고 그 양의 약 1/3량들이 각각 두부로 이행하였다.<br/> 2. 총 sucrose 량은 수침 시간을 연장함에 따라 약간씩 감소하여 두유로 이행되었으나 그 대부분은 순물로 이행되었고 두부로는 1/10량만이 이동되었다.<br/> 3. maltose는 수침 시간 연장에 별 관계없이 대부분이 두유로 이행되었다가 거의 전량 순물로 이행되었다.<br/> 4. raffinose는 수침 시간이 5, 10, 24시간으로 연장됨에 따라 대두 내의 총량에 비하여 55.6, 52.5 및 46.3%씩 각각 점차 감소하여 두유로 이행되었고 두유 내에 함유된 양의 약 20%가 두부로 이행되고 대부분 순물로 이동되었다.<br/> 5. 두부수율은 침지 시간을 5, 10, 24시간으로 연장시킴에 따라 차츰 증가하여 각각 37.6, 44및 47.4% 이었다. Changes in the soluble sugar content contained in the soybean, soybean milk, soybean curd and soybean whey were determined to investigate the method of reducing the introductions of soluble oligosaccharides like sucrose, raffinose and stachyose, which cause flatulence in human, to soybean curd from soybean by extending the soaking time of soybean.<br/> The soybean curd yields according to the extention of the soaking time were also determined.<br/> Markedly increased glucose, slightly reduced fructose and sucrose and noticeably reduced raffinose in soybean milk were observed with extended soaking time. Most of sucrose, raffinose and all of maltose were transferred to soybean whey.<br/> Gradual increase of soybean curd yields were obtained by extending soaking time within the experimental conditions.

돼지의 초산월령에 미치는 환경요인의 효과

최광수(Kwang Soo Choi),신원집(Won Jib Shin),백동훈(Dong Hoon Baik) 전북대학교 농업과학기술연구소 1990 농업생명과학연구 Vol.21 No.-

Retort Pouch 쌀밥의 加熱殺菌時 파우치 包裝內의 眞空度가 熱傳達 및 物性에 미치는 影響

최광수(Kwang Soo Choi),김창식(Chang Shik Kim) 한국식품영양과학회 1982 한국식품영양과학회지 Vol.11 No.4

retort pouch 쌀밥 제조 시간을 단축시키고 가열살균 중의 진공도가 열전달 속도 및 물성에 미치는 영향을 조사하기 위하여 본 연구를 실시하였다.<br/> 쌀의 수침시 평형수분 함량에 도달하는 시간이 품종에 별로 관계 없이 실온(24℃)에서는 2.5~3시간이고 증미를 실온에서 침지하여 약 60% 수분의 최적 쌀밥 수분함량에 도달하는 시간이 약 1시간이라는 것을 발견하였다.<br/> 111℃에서 49분간 살균했을때 고진공 포장에서의 Fo값은 3.16분이고 저진공포장 쌀밥의 2.90분으로써 고진공 포장에서의 살균값이 더 컸다. fh 값도 111℃ 살균시 고진공포장은 8.5분(j값=1.08), 저진공포장에서는 12.9분(j값=1.18)로서 고진공포장이 열전달 속도가 빠르다는 것을 알았고 121.1℃에서도 진공도의 영향은 같은 경향을 보였다. 고진공포장 쌀밥이 stickiness/Hardness의 비율로 봐서는 우수하게 보였으나 저진공포장의 쌀밥보다 stickiness, adhesiveness가 현저히 증가하고 쌀알이 파괴되고 엉켜서 품질이 좋지 않았다. Optimum moisture content of about 60% of cooked rice was obtained by soaking the fully steamed rice with 35% moisture conture content in ambient water temperature(24℃) for one hour.<br/> Two different levels of package vacuum was used in packing this cooked rice in retort pouch, and the effect of the levels of package vacuum on the texture of the cooked rice in the pouch during thermal process was investigated: Higher sterilization value (Fo value) and steeper slope (fh value) on the heating curve of the thermal process was obtained with the higher package vacuum product. The j value of the heating curves of cooked rice packed in retort pouch was ranged between 0.80 and 1.18 without respect to the levels of the package vacuum and the process temperature.<br/> Although in respective of the ratio of the stickiness to the hardness (-H/H) the product with higher package vacuum showed better result, the shape of most of the rice grain in the vacuum vacuum pouch was deformed and Jumped mass of inferior quality than the one in the lower vacuum package.

새로운 ZVS 소프트 스위칭 H-Bridge 인버터

최광수(Kwang-Soo Choi),정두용(Doo-Yong Jung),김재형(Jae-Hyung Kim),이수원(Su-Won Lee),원충연(Chung-Yuen Won),정용채(Yong-Chae Jung) 전력전자학회 2008 전력전자학술대회 논문집 Vol.- No.-

In this paper, we have proposed a novel zero-voltage -switching (ZVS) soft-switching H-bridge inverter. Because the conventional H-Bridge inverter generates switching losses at turn on and off, the efficiency is reduced. The proposed inverter operates ZVS switching using an auxiliary switch and resonant circuit to improve the efficiency. in the DC-DC converter stage, it can reduce not only switching loss but also capacity and size of passive devices due to the resonant elements. DC-AC inverter stage supplies load with energy through the ZVS operation of 4 switches. A detail mode analysis of operating is in presented. We have presented the inverter topology, principle of operation and simulation results obtained from the PSIM simulator.