생쥐 무릎관절 공간 발생에 있어 아포프토시스 세포에 관한 면역전자현미경적 연구

채희선,김경용,이원복,임형수,황덕호,장가용,Chae, Hee-Sun,Kim, Kyung-Yong,Lee, Won-Bok,Lim, Hyoung-Soo,Hwang, Douk-Ho,Chang, Ka-Yong 한국현미경학회 1998 Applied microscopy Vol.28 No.1

This study was designed to investigate the appearence and the characteristics of the apoptotic cells and the process of the joint cavity formation in mouse knee joint. Fetal mouse knee joints from 15 to 19 days of gestation were used. Paraffin-embedded serial sections, stained with H & E for light microscopic observation, Epon 812 embedded thin sections for electron microscopic observation and Lowicryl HM 20 embedded thin sections for immune-electron microscopic observation were prepared. Monoclonal antibodies to $\beta-tubulin$ and polyclonal antibodies to tissue transglutaminase were used for immune-electron microscopic study. The results obtained were as follows. 1. At 15 days of gestation, blood vessels, which have invaded in the mesenchymal cells, were present in the synovium, to form the joint cavity in the future. 2. At 16 days of gestation, the joint cleft was first appeared and several RBCs were present in the joint cleft. The invasion of blood vessels into the joint cleft was continuing, and apoptotic cells were present in the inner cell layer, adjacent to the joint cleft. Necrotic cells were also present in the outer cell layer; they were present 18 days of gestation, but apoptotic cells did not appear after 17 days of gestation. 3. In the apoptotic cells, transglutaminase were localized around vacuoles and the marginal site of the cytoplasm. 4. In the apoptotic cells, tubulin was around the endoplasmic reticulum and the marginal site of the cytoplasm. In the late stage of apoptotic cells, tubulin was localized diffusely in the cytoplasm. Tubulin was also strongly labeled around in the cytoplasm of the neighboring cell at which the apoptotic body was phagocytosed. Tubulin labeled particles were apparently increased in the seperated apoptotic bodies. On the basis of the above findings, it is proposed that during the development of the mouse knee joint, blood vessel invasion first occurs and then apoptosis and cell necrosis follow it. In the apoptotic cell, present in the synovium of the developing knee joint of the mouse. it is suggested that the redistribution of tubulin is associated with apoptotic process. And transglutaminase overexpressed in the apoptotic cell.

Pulsed-field gel electrophoresis(PFGE)를 이용한 Listeria monocytogenes의 molecular typing

채희선 ( Hee Sun Chae ),김주영 ( Ju Young Kim ),김연하 ( Yoen Ha Kim ),양윤모 ( Yun Mo Yang ),진경선 ( Kyong Sun Jin ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.3

A total of 1,354 samples was collected from bovine and porcine carcass from January 2005 to December 2006 in a slaughter house. Twenty five strains(1.8%) of L monocytogenes were isolated from 1,354 samples using selective media. Ten(1.4%) L monocytogenes were isolated from the 677 of bovine carcasses, and 15(2.2%) were isolated from the 677 of porcine carcasses. Among 15 L monocytogenes from porcine, 11 siolates were serovars 1/2c, followed by 1/2b (3 strains, 20.0%) and 1/2a(1 strain) Out of 10 bovine samples, positive cases in 1/2a were 9 strains (90.0%), 1/2b were 1 strains(10.0%). PCR primers were selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene (hlyA) of L monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested. A total of 25 L monocytogenes strains were analysed by PFGE after digestion with ApaⅠ. PFGE analysis of genomic DNA showed the 14~18 fragments ranging in size from 30 to 550 kb, resulting in 14 patterns.

소의 분변과 도체에서 shiga toxin-producing Escherichia coli의 분리와 특성

채희선 ( Hee Sun Chae ),김능희 ( Neung Hee Kim ),한혜진 ( Hye Jin Han ),손홍락 ( Hong Rak Son ),김창기 ( Chang Ki Kim ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ),김종택 ( Jong Taek Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains can cause broad spectrum of human disease, including diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic colitis (HUS). We examined 868 samples was taken from bovine feces and carcass from January to December 2008 in Seoul. Twenty two (9.5%) shiga toxin -producing Escherichia coli were isolated from the 230 of bovine feces, and two (0.31%) were isolated from the 638 of carcasses. Serotype of E. coli isolates were O157 (10, 41.6%), O26 (10, 41.6%), O111 (1, 4.2%) and UT (3, 12.6%). In PCR, the isolates displayed three different stx gene combination (stx1〔2, 8.4%〕], stx2〔3, 12.6%〕and stx1 and stx2〔19, 87.5%〕). The eaeA and hlyA gene were found in 11 (45.8%) of the 24 strains. Saa gene was present only one strains (4.2%). Toxin typing using reverse passive latex agglutination test showed the same result in VT 1. But it showed different result in VT 2. In antimicrobial susceptibility test, all isolates were sensitive to amikacin, amoxicillin/clavulanic acid, ciprofloxacin and colistin. Eighteen strains (75.0%) of 24 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty four isolates was digested with Xba I. the 24 isolates showed 7 (A~G) PFGE type.

소 분변과 도체에서 E coli O157:H7의 분리와 항생제 감수성

채희선 ( Hee Sun Chae ),김종화 ( Jong Hwa Kim ),김규현 ( Gyu Hyeon Kim ),최태석 ( Tae Seok Choi ),신방우 ( Bang Woo Shin ),이덕주 ( Duck Joo Lee ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.1

In this study, a total of 2,119 samples was taken from bovine feces and carcass from March 2002 to December 2003. And those were examined for the presence of enterohemorrhagic E coli O157:H7. The properties of the isolates were characterized for biochemical features, serotypes, virulence genes and antimicrobial susceptibility. Forty five strains(3.7%) of E coli O157:H7 were isolated from 1,208 fecal samples and were not detected in carcass using immunomagnetic separation technique and selective media. In multiplex PCR using stx1, stx2, eaeA and hlyA primers, the amplified bands at 180bp, 255bp, 384bp and 534bp were observed, respectively. In antimicrobial susceptibility test, all isolates were susceptible to amoxicillin/clavulanic acid and cefazolin. The isolates were most resistant to sulfisoxazole(24.4%), followed by streptomycin(22.2%), tetracycline(20.0%). Eight strains(17.8%) of 45 isolates showed the multi-resistant patterns with over 3 drugs.

수생조류에서 Clostridium botulinum type C에 의한 발증사례

채희선 ( Hee Sun Chae ),김능희 ( Neung Hee Kim ),손홍락 ( Hong Rak Son ),김창기 ( Chang Ki Kim ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4

Botulism in wild waterfowl has been reported in many areas of the world. In 18 October 2008 to 22, 101 dead wild ducks were found in Anyangcheon. 24 of dead birds were spotbill (Anas poeilorhyncha), 56 were Mallard (Anas platyrhynchos), 18 were green- winged teal (Anas carolinensis) and 3 were others. Clinical sign of the sick birds were flaccid paralysis of the neck. At autopsy, most birds had no specific lesions. Stomach is empty. In the mouse bioassay, Samples of serum and intestinal contents taken from 9 dead birds killed all the injected mice, and their toxicity was neutralized by the antitoxin against C. botulinum type C toxin.

전문직 비정규직이 팀 내 수용 및 창의적 팀 성과에 미치는 영향에 관한 탐색적 연구 : 경계 없는 비정규직과 과도기적 비정규직을 중심으로

채희선(Hee-Sun Chae),박지성(Ji-Sung Park) 강원대학교 경영경제연구소 2020 Asia-Pacific Journal of Business Vol.11 No.2

Purpose - To understand the growing interests in the professional temporary workforce, this study aimed to examine the different types of professional temporary workers and their different effects on creative performance. Design/methodology/approach - By sub-classifying professional temporary workers as boundaryless workers and transitional workers, this study developed propositions to provide comprehensive framework for directing meaningful topics for future research on professional temporary workers. Findings - This study suggested that two types of professional temporary workers may differ significantly in terms of their work efforts for getting approval or acceptance from the team members and for achieving creative contributions in teams. Specifically, drawing on expectance theory, social identity theory and resource-based theory, this study discussed the relationship between acceptance(task-oriented vs. relational-oriented acceptance), employment duration(short-term status vs. long-term status), contract security(volition being temporary worker) and creative performance for boundaryless workers versus transitional workers. Research implications or Originality - This study have implications for increasing our understanding of professional temporary workers by showing that creative performance may vary by employment types.

북한산 국립공원 주변지역 사육견의 광견병 항체 분포조사(2)

채희선 ( Hee Sun Chae ),소병재 ( Byung Jae So ),김두환 ( Doo Hwan Kim ),조미영 ( Mee Young Jo ),배니수 ( Nae Soo Bae ),기노준 ( No Joon Ki ),이병동 ( Byung Dong Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.1

Recently, the rabies cases have been reported in Paju-and Yangju-gun, Gyeonggi province near Seoul metropolitan area. The Pukhansan national park, nearly located from the cities, is suspected to be a high risk area for incidence and spread of the rabies to metropolitan area. This study was conducted to investigate the prevalence rate for rabies antibody of dogs near the Pukhansan national park and in some other districts in Seoul metropolitan city. From march to october 2001, a total of 306 serum samples were taken from dogs for breeding(189) and pet dogs(117) in 4 districts near the Pukhansan national park and other districts of Seoul. Rabies virus antibodies in sera were detected by neutralizing peroxidase-linked assay(NPLA). Of the 306 sera of dogs tested, 74(24.2%) were positive to rabies virus antibody. The prevalence rates of rabies antibody in Pukhansan national park area and in the other districts of Seoul city were 23.7% and 25.3%, respectively. There was no significant difference in the prevalence rate between these two districts. The prevalence rates of rabies antibody in pet dogs and dogs for breeding were 40.2% and 14.3% respectively. The prevalence rates of rabies antibodies in less than 1 year, 1~<2 years, 2~<3 years, and over 3 years old dogs were 14.5%, 22.4%, 32.6%, and 27.1%, respectively, and overall 24.2% in the dog population. In addition, we found that dogs less than 1 year old had lower antibody prevalence than those over 1 year old. It was concluded that enhancement of vaccination is important in the prevention of the rabies, and that rabies vaccines should not be less supplied than the population of the dog.

자기 비판적 완벽주의와 조직시민행동 간 관계: 성별의 조절효과

채희선(Heesun Chae),박지성(Jisung Park) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.2

본 연구는 개인 수준에서 조직시민행동이 초래하는 딜레마적 상황을 보다 잘 이해하기 위해서, 조직시민행동에 영향을 미치는 개인적 특성인 자기 비판적 완벽주의 성격과 성별효과를 검증하고자 한다. 즉, 자원보존이론을 바탕으로 자기 비판적 완벽주의 성격이 조직시민행동에 미치는 영향에 대해 논의하고, 성별 차이가 이 관계를 어떻게 변화 시키는지를 살펴보고자 한다. 본 연구에서는 자원보존이론에서 주장하듯이, 자기 비판적 완벽주의 성격은 조직시민행동에 부정적인 영향을 줄 것으로 예측하였다. 또한 성별 관련 논의들에 근거하여 여성에 비해 남성일 경우 자기 비판적 완벽주의와 조직시민행동 간 부(-)의 관계가 강하게 나타날 것임을 가설로 제시하였다. 이러한 가설들을 검증하기 위하여 한국 기업들을 대상으로 상사-부하 설문을 실시하였고, 총 150쌍의 데이터를 사용하여 위계적 선형모형 분석을 실시하였다. 분석결과 자기 비판적 완벽주의 성격과 조직시민행동 간에 부(-)적인 영향이 있음을 확인 하였고, 이러한 부적 효과는 남성의 경우 더 강하게 나타남을 확인 하였다. 본 연구는 조직시민행동을 설명하는 개인적 특성과 성별효과를 살펴봄으로 인해, 조직시민행동 관련된 분야에 이론적·실무적 함의를 제공한다고 할 수 있다. This study examined the effects of self-critical perfectionism on organizational citizenship behavior (OCB) and the moderating effects of gender in the relationship to understand deeply the dilemmatic situation resulting from OCB at the individual level. In other words, based on the conservation of resources theory, this study examined how self-critical perfectionism influences the OCB and how gender moderates the relationship between self-critical perfectionism and OCB. More specifically, this study predicts that self-perfectionism will be negatively related to OCB. Furthermore, this negative relationship between self-critical perfectionism and OCB will be strengthened in men compared to women. To test these hypotheses, the supervisor-subordinate survey was conducted, and 150 dyad data were analyzed. The analytical results of HLM (Hierarchical Linear Modeling) showed that self-critical perfectionism negatively influenced the OCB, and this negative effect was strengthened in men compared to women. This paper suggests implications and limitations for the literature on OCB.

주도적 성격과 창의적 행위: 사회적 상호작용과 상사의 비인격적 행위의 조절효과

채희선 ( Heesun Chae ),박지성 ( Jisung Park ) 한국윤리경영학회(구 한국기업윤리학회) 2020 윤리경영연구 Vol.20 No.2

본 연구는 주도적 성격과 창의적 행위와의 관계를 보다 잘 이해하기 위해서 사회적 경계조건으로 상사와 동료의 영향력을 살펴보았다. 즉, 주도적 성격이 가지는 특징을 활성화 시키는 상황변수로 동료들 간의 사회적 상호작용을 고려하였고, 주도적 성격이 가지는 특징을 제약하는 상황변수로는 상사의 비인격적 행동을 살펴보았다. 이러한 조절 효과를 확인하기 위해서 다양한 산업군의 조직에서 근무하는 248명의 조직구성원-상사설문을 통해 확보한 데이터를 분석하였다. 분석 결과, 주도적 성격과 창의적 행위 간의정(+)적인 관계를 확인하였으며, 이러한 정(+)의 관계가 가장 강하게 나타날 때는 상사의 비인격적 행위가 낮고 동료들 간의 사회적 상호작용이 높을 때임을 확인할 수 있었다. 본 연구의 결과를 바탕으로 결론에서는 창의적 행위에 영향을 미치는 성격적 요인에 대한 추가적인 논의와 상황적 변수의 중요성에 대해서 이론적ㆍ실무적 시사점을 논의하였다. This study examines the effects of leaders and coworkers on the relationship between a proactive personality and creative behavior as social boundaries. More specifically, this study considers social interactions among coworkers as a contingent variable to stimulate the characteristics of a proactive personality. By contrast, this study also investigates a leader’s abusive supervision as a contextual variable to constrain the activation of a proactive personality. To test these moderating effects, this study analyzed 248 employees through leader dyad data collected from various industries. The analytical results showed that a proactive personality was positively related to creative behavior. In addition, this positive relationship was highly pronounced when social interactions among coworkers were high and a leader’s abusive supervision was low. Based on these findings, this study provides theoretical and practical implications and limitations in the personality and creativity literature.

세라마이드로 유발된 신경세포사멸에서 에스트로젠의 Bax 발현 억제를 통한 보호작용

채희선(Hee-Sun Chae),김성수(Sung Su Kim),김경용(Kyung Yong Kim),이원복(Won Bok Lee) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.6

In the present study, we investigated effects of estrogen on cell death induced by ceramide in human neuroblastoma SK-N-SH. Estrogen, attenuated ceramide-induced cell death. To determine the molecular mechanism of protective effect of estrogen, we investigated the effect of estrogen on the increase of Bax by ceramide. Estrogen decreased Bax expression. Moreover, estrogen showed the inhibitory effect on the caspase activation by ceramide. These results suggest that estrogen attenuate ceramide-induced neuronal cell death by downregulation of Bax and subsequent inhibition of caspase activation. 본 연구는 세라마이드에 의해 유발된 사람 신경모세포종 SK-N-SH의 세포사멸에 대한 에스트로젠의 효과를 알아보았 다. 에스트로젠은 세라마이드에 의한 세포사멸을 효과적으로 억제하였다. 세라마이드로 유발된 세포사멸에 대한 에스트로젠의 보호작용과 Bax 발현 변화와의 관련성을 규명하기 위하여 세라마이드에 의한 Bax 단백질의 발현에 대한 에스트로젠의 영향을 측정하였다. 그 결과 에스트로젠은 세라마이드에 의해 증가된 Bax 단백질의 발현수준 증가를 억제하였다. 또한 에스트로젠이 caspase의 활성도 억제하는 것으로 나타났다. 이러한 결과는 에스트로젠이 Bax 발현을 억제하고 이후 caspase의 활성 또한 억제하는 신호전달과정을 통하여 세라마이드에 의한 세포사멸에 대해 보호효과를 나타내고 있음을 제시해 주는 것이라 하겠다.