Influence of pretreatment of insulin on phosphorylation of extracellular receptor kinase by gonadotropin releasing hormone in cultured mouse granulosa cells

채희동 ( Hee Dong Chae ),( Young Jin Lee ),( Il Kyung Jeon ),( Ji Won Lee ),( Eu Na Park ),( Ji Sun Kim ),( Yun Hee Koo ),( Sung Hoon Kim ),( Chung Hoon Kim ),( Byung Moon Kang ) 대한산부인과학회 2007 대한산부인과학회 학술대회 Vol.93 No.-

종양괴사인자-α가 배양된 인간 황체화과립막세포의 에스트라디올, 프로게스테론, 인슐린유사성장인자-II, 인슐린유사성장인자 결합단백질-1, 2, 3의 분비에 미치는 영향

채희동(Hee Dong Chae),강은희(Eun Hee Kang),박은주(Eun Ju Park),김성훈(Sung Hoon Kim),홍석호(Seok Ho Hong),김정훈(Chung Hoon Kim),강병문(Byung Moon Kang),장윤석(Yoon Seok Chang),김정구(Jung Gu Kim),이진용(Jing Yong Lee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.4

N/A Objectives : To investigate the influence of TNF-α on the secretion of estradiol (E2), progesterone (P4), insulin-like growth factor (IGF)-II, insulin-like growth factor binding protein (IGFBP)-1, 2, and 3 in cultured human luteinized granulosa cells. Materials and Methods : Human luteinized granulosa cells were obtained from the follicular fluid by transvaginal oocyte aspiration from infertile patients undergoing controlled ovarian hyperstimulation (COH) for in vitro fertilization (IVF). The cells were grouped into the control, 1.0, 10.0, and 100.0 ng/ml of TNF-α group according to the concentrations of TNF-α. The cells were cultured for 72 hours with the different concentrations of TNF-α as descibed above. The cells not treated with TNF-α served as control. The concentrations of E2, P4, IGF-I, IGFBP-1, 2, and 3 were determined in conditioned culture media by immunoradiometric assay (IRMA) or radioimmunoassay (RIA). Results : The cell number in 100.0 ng/ml of TNF-α group was significantly higher than those in other groups, although the cell viabilities were similar in all groups. There were no statistically significant differences in the concentrations of E2 in all groups. However, the concentrations of P4 were seemed to be decreased as the concentrations of TNF-α were increased and the concentration of P4 in 100.0 ng/ml of TNF-α group was significantly lower than those in the control and other TNF-α groups. The concentrations of IGF-II, IGFBP-1, 2, and 3 were not different among the control and each TNF-α group. The secretion of E2 and P4 was not affected by IGF type I receptor antibody pretreatment. Conclusion : TNF-α might play a role as a regulator of ovarian physiology by modulating luteinized granulosa cellular proliferation and P4 secretion, and this mechanism might not be related to IGF system.

배양된 생쥐의 과립막 세포에서 activin과 follistatin이 인슐린유사성장인자-1, 2, 인슐린유사성장인자 결합단백질-1, 2, 3의 mRNA 발현에 미치는 영향

채희동 ( Hee Dong Chae ),홍석호 ( Seok Ho Hong ),조윤현 ( Yun Hyun Cho ),오영미 ( Young Mi Oh ),이방현 ( Bang Hyun Lee ),김성훈 ( Sung Hoon Kim ),김정훈 ( Chung Hoon Kim ),강병문 ( Byung Moon Kang ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.3

목적 : Activin과 inhibin이 배양된 생쥐의 과립막 세포에서 인슐린유사성장인자 (insulin-like growth factor, IGF)-I, Ⅱ와 인슐린유사성장인자 결합단백질 (insulin-like growth factor binding protein, IGFBP)-1, 2, 3의 mRNA 발현에 어떠한 영향을 미치는 가에 대하여 알아보고자 본 연구를 시작하였다. 연구 재료 및 방법 : ICR 생쥐의 암컷에서 난소를 채취하고 과립막 세포 Objective : To investigate the influence of activin and follistatin on the expression of IGF (insulin-like growth factor)-I, Ⅱ, IGFBP (insulin-like growth factor binding protein)-1, 2, and 3 mRNA in cultured mouse granulosa cells Materials and Methods : T

자궁강내 인공수정시 사용되는 카테타에 따른 임상 결과의 비교

채희동(Hee Dong Chae),강은희(Eun Hee Kang),김정훈(Chung Hoon Kim),강병문(Byung Moon Kang),장윤석(Yoon Seok Chang) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.2

목적: 자궁강내 인공수정(intrauterine insemination, IUI)을 시행함에 있어 사용되는 카테타의 종류가 임상 결과에 어떠한 영향을 미칠 수 있는 가를 알아보고자 하였다. 연구 대상 및 방법: 1998년 3월부터 1998년 9월까지 울산대학교 의과대학 서울중앙병원 산부인과 불임클리닉을 방문하여 IUI를 시행받은 95명을 대상으로 하였다. 대상 환자들은 사용된 카테타의 종류에 따라 TomCat group(n = 39)과 Mackler group(n = 56)으로 분류되었다. IUI를 위한 과배란유도에는 성선자극호르몬분비호르몬 작용제(GnRH-a)를 사용한 황체기 장기투여법(luteal phase long protocol)이 사용되었다. 통계학적 유의성은 Student's t-test, χ2 test, 그리고 Fisher's exact test 등으로 검증하였다. 연구 결과: 양 군간에 과배란유도를 위하여 사용된 외인성 성선자극호르몬의 용량과 기간은 유의한 차이가 없었다. 또, hCG 투여일의 혈중 에스트라디올 농도, 자궁 내막의 성상 및 두께에 있어서도 양 군간 유사한 결과를 보였다. 그러나, 시술후 출혈의 빈도는 TomCat group에서 Mackler group에 비하여 다소 높은 것으로 나타났으며(15.4% vs. 3.6%, p = 0.06), 처음 시술시 카테타의 삽입이 어려워 sound를 해야 했던 경우도 TomCat group에서 Mackler group과 비교할 때 유의하게 높은 빈도로 나타났다(23.1% vs. 0%, p < 0.01). 한편, 환자당 임상적 임신율은 양 군간에 유의한 차이가 없었다. 결론: IUI를 시행함에 있어서, 특히 자궁경부인자 불임 환자의 경우라면, Mackler 카테타를 이용하는 것은 임상적으로 유용할 수 있으리라고 추측된다. Objective: To compare the clinical outcomes of intrauterine insemination (IUI) according to the catheter used. Materials and Method: From March 1998 to September 1998, total 95 infertile patients were included in this study. Patients were randomly allocated to TomCat group (n = 39) and Mackler group (n = 56) according to the catheter for insemination. The controlled ovarian hyperstimulation (COH) using luteal long protocol of gonadotropin releasing hormone agonist (GnRH-a) was used in all patients. Statistical analysis was performed using Student's t-test, Fisher's exact test, and χ2 test as appropriate. Statistical significance was defined as p < 0.05. Results: The total dose and duration of exogeneous gonadotropin required were similar between the two groups. There were also no significant differences in serum estradiol (E2) level, endometrial thickness and texture on the day of hCG administration between the two groups. However, the percentage of uterine souding due to failure of initial approach was significantly higher in TomCat group compared to Mackler group (23.1% vs. 0%, p < 0.01). The percentage of bleeding after IUI in TomCat group seemed to be higher than that in Mackler group (15.4% vs. 3.6%, p = 0.06), although there was no statistically significant difference between the two groups. There was also no significant difference in the clinical pregnancy rate per patient between the two groups. Conclusion: These results suggested that using Mackler catheter might be effective for IUI, especially for the patients with cervical factor infertility.

Influence of TNF-α on the Expression of IGF-2, IGFBP-1, 2, and 3 mRNA in Cultured Human Luteinized Granulosa Cells

채희동 ( Hee Dong Chae ),( Seok Ho Hong ),( Young Mi Oh ),( Bang Hyun Lee ),( Sung Hoon Kim ),( Chung Hoon Kim ),( Byung Moon Kang ) 대한산부인과학회 2002 대한산부인과학회 학술대회 Vol.88 No.-

자궁근종과 생식력

김소라,채희동,Kim, So-Ra,Chae, Hee-Dong 대한생식의학회 2010 Clinical and Experimental Reproductive Medicine Vol.37 No.3

자궁근종은 환자마다 다양한 조성, 크기, 위치, 개수를 가지는 양성 종양으로 여러 가지 방법으로 생식력에 영향을 준다. 자궁근종이 생식률에 미치는 영향에 대한 연구는 많이 시행되어 왔지만 여전히 명확한 결론을 내리기는 어렵다. 자궁근종의 위치와 크기는 향후 임신에 영향을 미치는 가장 중요한 인자이다. 장막하근종은 임신에 거의 영향을 주지 않으나 자궁내강의 모양을 변형시키는 점막하근종과 근층내근종은 임신율을 감소시키며 자연 유산율을 증가시킨다. 그러므로 보조생식술을 시행하기 전에는 먼저 제거하는 치료가 충분히 상의되어야 한다. 자궁내강을 변형시키지 않은 근층내근종이 있는 환자도 임신율의 저하를 보였지만, 확실한 연구가 없기 때문에 결론을 내리기가 쉽지 않다. 근층내근종의 치료로 근종절제술이 생식력을 향상시킨다는 증거는 아직 불충분하므로 환자의 상태와 의사의 경험에 따라 치료 방법을 결정하여야 한다. Uterine myomas are heterogeneous tumors in composition, size, location, and number; variation in any of these factors could possibly alter the effect on a woman's fertility status. The effect of myomas on fertility has been the subject of many studies. However, a definitive answer is still missing. The location and size of the myomas are the two parameters that influence the success of a future pregnancy. Subserosal myomas seem to have little effect on reproductive outcome. Myomas that compress the uterine cavity with an intramural portion and submucosal myomas significantly reduce pregnancy rates, and should be removed before assisted reproductive techniques are performed. Patients with intramural myomas also may have a poorer reproductive outcome, but the lacks of quality evaluations make this conclusion tenuous at best. Removal of myomas with an intra-cavitary component seems to be of benefit. However there are as yet no data to support myomectomy in the treatment of intramural myomas to improve fertility outcomes. Treatment modality for myomas located at intramural sites should be determined according to clinical status of the patient and doctor's experience.

다낭성난소증후군 환자들에서 Clomiphene Citrate에 대한 난소 반응의 예측 인자들에 관한 연구

김대진,채희동,손철,김정훈,강병문,장윤석,목정은,Kim, Dae-Jin,Chae, Hee-Dong,Sohn, Cherl,Kim, Chung-Hoon,Kang, Byung-Moon,Chang, Yoon-Seok,Mok, Jung-Eun 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

Objectives: To determine whether the body weight, body mass index (BMI), and basal serum level of LH, FSH, testosterone (T), dehydroepiandrosterone sulfate (DHEA-S) are related to the ovarian response to clomiphene citrate (CC) in patients with polycystic ovarian syndrome (PCOS). Materials and Method: From January 1996 to June 1997, total 57 patients with PCOS were enrolled in the present study. Women who had other infertility factors were excluded from our study. The ovulation induction using CC was used in all patients. The patients were grouped into 50 mg group, 100 mg group, and 150 mg group according to their daily CC dose. The patients were also grouped to ovulatory and non-ovulatory group. The body weight, BMI, and basal serum level of LH, FSH, T, DHEA-S were measured in all patients on the 2nd or 3rd day of the menstrual cycle. Results were analysed with Student's t-test and Fisher's exact test. Results: The body weight and BMI of the nonovulating group were significantly higher than those of the ovulating group in all groups (50, 100, 150 mg of CC). However, there were no significant differences of the level of LH and FSH between ovulating and nonovulating groups in all CC groups (50, 100, 150 mg). The level of T of nonovulating group was significantly higher in 50 and 100 mg of CC groups, but not in 150 mg group. The level of DHEA-S of the non-ovulating group is significantly higher in 50 mg group, but not in 100 and 150 mg groups. Conclusion: The body weight and BMI could be useful predictors of ovarian response to CC in patients with PCOS, and basal T and DHEA-S also might be useful in cases of low-dose CC treatment.

Clomiphene Citrate 부하검사와 난소 반응 예측 인자와의 연관성에 관한 연구

문신용,채희동,김광례,서창석,김석현,최영민,신창재,김정구,이진용,Moon, Shin-Yong,Chae, Hee-Dong,Kim, Kwang-Rye,Suh, Chang-Suk,Kim, Seok-Hyun,Choi, Young-Min,Shin, Chang-Jae,Kim, Jung-Gu,Lee, Jin-Yong 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.3

Objective: To determine the cutoff value of clomiphene citrate challenge test(CCCT) that can predict the normal and abnormal(diminished) ovarian response group and to assess the usefulness of CCCT as a predictor of ovarian reserve. Materials and Methods: From March 1994 to Februry 1996, CCCT was performed to 129 infertile patients and among them, 20 patients whose basal FSH on the third day of menstrual cycle was more than 20 mIU/ml were excluded. At the same time, the same CCCT was performed to the fifteen healthy volunteers with proven fertility to determine the cutoff value of CCCT. Results; 1) A FSH value higher than 23.4 mIU/ml, measured on the 10th day of menstrual cycle, was defined as a abnormal ovarian response. The cutoff value of 23.4 mIU/ml is more than 2 standard deviations(SD) above the mean value of 15 healthy women after CCCT. 2) The abnormal CCCT group, the subpopulation with a FSH value of 23.4 mIU/ml or more, was 7.3%(8/109) and their mean age was higher than the normal CCCT group($36.5{\pm}4.5$ vs. $32.9{\pm}4.8$, P = 0.059). And the percentage of the patients older than 35 years of the abnormal CCCT group was significantly higher than that of the normal CCCT group(62.5% vs. 38.6%, p <0.05). 3) There was no correlation between the hormone values of the third day and the 10th day of menstrual cycle before and after CCCT except between FSH of the third day and the 10th day. Conclusion: The CCCT is a good method to predict the individual ovarian response to COH for ART, especially in the patients who has no other abnormal findings that predict poor prognosis. And it is neccessary to determine the cutoff value of CCCT by the large numbers of randomized study, and combining the previously proven prognostic factors, it can be applicated in many individual centers for evaluate the ovarian response before ART program.

체외수정시술시 예후 인자로서 정자 첨체반응 유발검사의 유용성

김정훈,채희동,강은희,추형식,전용필,강병문,장윤석,목정은,Kim, Chung-Hoon,Chae, Hee-Dong,Kang, Eun-Hee,Chu, Hyung-Sik,Cheon, Yong-Pil,Kang, Byung-Moon,Chang, Yoon-Seok,Mok, Jung-Eun 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

It is well known that the clinical test for responsibility of accurate fertilization capacity in male partners is very important to diagnose and treat the infertility. However, it has been reported that the traditional semen analysis cannot accurately predict fertilization and pregnancy potential. The present study was performed to evaluate the acrosomal reaction to ionophore challenge (ARIC) test as a prognostic indicator for fertilization of sperm and oocyte in an in vitro fertilization and embryo transfer (IVF-ET) program. From March 1996 to Februry 1997, 30 couples undergoing IVF program were allocated to this study group. All female partners in the study group were 35 years old or less and their serum level of basal follicle stimulating hormone (FSH) and estradiol $(E_2)$ were normal. All the male partners have normal parameters of semen analysis. The ARIC tests were performed on the day of ovum pick up and in vitro insemination in all the male partners. The controlled ovarian hyperstimulation (COH) using luteal long protocol of gonadotropin releasing hormone (GnRH) agonist was used in all couples for IVF-ET. The acrosomal reaction with $10{\mu}l$ of 10% DMSO was induced spontaneously in $10.1{\pm}9.8%$, and acrosomal reaction with calcium ionophore A 23187 was induced in $27.4{\pm}18.1%$, and the ARIC value was $17.4{\pm}16.2%$. There were no significant correlation between the ARIC value and the fertilization rate ($r^2$=0.044, p=0.268). There were also no significant correlation between the ARIC value and the percentage of the grade I, II embryos ($r^2$=0.046, p=0.261). On the basis of above results, it was suggested that ARIC test might not be a useful prognostic indicator for fertilization in IVF-ET in male partners with normal parameters of conventional semen analysis. We guessed that IVF-ET could be performed to the patients primarily without universal appilcation of ARIC test to all male partenrs, and if fertilization failure occurs, the micro assisted fertilization (MAF) such as intracytoplsmic sperm injection (ICSI) might be used as an alternative mode of treatment with acceptable success rate.

흰쥐 자궁 상피와 내막에서 기원한 세포주의 체외배양

강병문,이석원,채희동,강은희,추형식,김정훈,장윤석,남주현,Kang, Byung-Moon,Lee, Suk-Won,Chae, Hee-Dong,Kang, Eun-Hee,Chu, Hyung-Sik,Kim, Chung-Hoon,Chang, Yoon-Seok,Nam, Joo-Hyun 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1

Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.