유독성 4-Chlorobiphenyl의 생분해를 위한 탈염소화 유전자의 클로닝

김치경,채종찬,한재진,Kim, Chi-Kyung,Chae, Jong-Chan,Han, Jae-Jin 한국미생물학회 1994 미생물학회지 Vol.32 No.2

4-Chlorobiphebyl(4CB)를 분해한 Pseudomonas sp. DJ-12가 가지고 있는 pcbABCD 유전자를 Escherichia coli에 클로닝한 결과, 재조합 균주인 E. coli CU1과 CU101은 모두 Pseudomonas sp. DJ-12에서와 같이 4CB를 분해하여 2,3-dihydrozybiphenyl(2,3-DHBP)을 생성하는 탈염소화 기능을 보여주었다. 특히 pcbAB를 포함하는 재조합 플라스미드인 pCU101을 가지고 있는 E. coli CU101은 Pserudomonas sp. DJ-12에서와 같이 4CB로부터 4-chlorobenzoic acid를 생성하지 않고 2, 3-DHBP만을 생성하는 탈염소화 기능을 보여주었다. 그러므로 Pseudomonas sp. DJ-12의 염색체 DNA로부터 클로닝한 약 2.2kb의 pcbAB 유전자는 4CB로부터 2,3-DHBP만을 생성하는 탈염소화기능을 가지고 있음이 밝혀졌다. The pchABCD genes in Pseudomonas sp. DJ-12 speciyin degradation o 4-chlorobiphenyl(4CB) were cloned in Eschericia coli. The cloned cells of E. coli CU1 and CU101 showed to produce 2,3-dihydroxybiphenyl (2,3-DHBP) from 4-chlorobiphenyl by dechlorination, as Pseudomonas so. DJ-12 produced 2,3-DHBP from both biphenyl and 4CB. In particular, E. coli CU101 transformed with the recombinant plasmid of pCU101 revealed dechlorination activity to produce 2,3-DHBP from 4CB without production of 4-chlorobenzoic acid. Therefore, the pcbAB genes (2.2 kb in size) cloned from the chromosome of Pseudomonas sp. DJ-12 were found to have dechlorination activity on 4CB to produce 2,3-DHNP.

원발성 간암과 췌장암에 있어서의 αFP , TATI 및 CA 19 - 9 의 임상적 의의

이상종(Sang Jong Lee),이만호(Man Ho Lee),이승세(Seung Sei Lee),서영주(Young Joo Suh),채종찬(Jong Chan Chae),정을순(Eul Soon Jung) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3

N/A The aim of this study was to evaluate the usefulness of measuring more than two tumor markers including TATI in the diagnosis of pancreatic cancer and hepatoma. Pancreatic cancer patients (10); The range of serum CA 19-9 concentration was 55.01-231 U/ml. All of 10 (100%) had increased value (>33U/ml). Nine of 10 patients (90%) had CA 19-9 values above the cancer diagnostic cut off level (>100U/ml). Serum TATI concentration were raised (range 26.47-450 pg/,L ) in 80% (8/10) of the patients. Serum TATI concentration was above cut off level (40pg/I ) in 5 patients (50%). One patient, who had non diagnostic CA19-9 level (42.9 U/ml), had 413.47 pg/Lof serum TATI concentration. Thus when the two tumor markers (CA19-9, TATI) were used together, all of 10 pancreatic cancer patients (100%) had diagnostic cut off level. Primary hepatoma patients (22); Serum aFP value were raised in 86.36% (19/22) of the patients (range 14.26-2014 pg/ml). Six patients had value of non diagnostic range (14.26-237 pg/ml), so that 59. 09% (13/22) of the patients had a diagnostic aFP value (>400pg/ml). Serum TATI values were raised in 77.2% (17/22) of the patients. 59% (13/22) of the patients had serum TATI value above the cut off level. Of the 6 patients who had non diagnostic a FP level, three patients had serum TATI value above cut off level. When serum n FP and TATI were measured together, 81.8% (18/22) of the patients had at least one of the value above cut off level. So if the two tumor markers were used together (CA19-9 and TATI in pancreatic cancer, aFP and TATI in primary hepatoma) it might be helpful in some patients in the diagnosis of malignancy. But accounting economical and other factors, we think it needs further evaluation.

Genome sequence of Ochrobactrum anthropi AM3 isolated from compost

이승제,박수제,채종찬,Patil, Kishor Sureshbhai,Lee, Seung Je,Park, Soo-Je,Chae, Jong-Chan 한국미생물학회 2016 미생물학회지 Vol.52 No.4

단일 탄소원과 에너지원으로 리그닌을 이용하여 성장할 수 있는 Ochrobactrum anthropi AM3 균주를 퇴비로부터 분리하였다. 본 연구에서는 AM3 균주로부터 56.2% G+C 함량의 약 5.11 Mb 크기 유전체 염기서열을 결정하였으며 연구 결과는 Ochrobactrum속의 유전적 다양성과 리그닌 분해기작 연구를 위한 유전체 정보를 제공한다. Ochrobactrum anthropi AM3 was isolated for the ability to utilize lignin as a sole carbon and energy source from compost in South Korea. Here we report the 5.11 Mb draft genome of strain AM3 with a G+C content of 56.2%, which is helpful for understanding the genetic diversity among Ochrobactrum spp. and the mechanism of lignin degradation.

Pseudomonas sp. G19에 의한 배추의 염 스트레스 경감 및 생장 촉진

이건웅,이귀재,채종찬,Lee, Gun Woong,Lee, Kui-Jae,Chae, Jong-Chan 한국미생물학회 2014 미생물학회지 Vol.50 No.4

염 스트레스를 비롯한 다양한 비생물학적 스트레스는 식물의 생장저해와 작물 생산량을 감소시키는 요인으로 작용한다. 계화도 간척지 토양에서 식물생장 촉진 세균을 분리하여 Pseudomonas sp. G19로 명명하였다. G19 균주는 36시간 배양 후 $7.5{\mu}g/ml$의 indole acetic acid를 생산하고 불용성인산을 25% 가용화시켰으며, 식물의 에틸렌 감소와 관련된 1-aminocyclopropane-1-carboxylate deaminase를 발현하였다. 또한 150 mM NaCl에 침지된 염 조건의 토양에서 재배된 유묘기 배추를 이용한 실증실험에서 G19 균주는 배추의 생체중량을 증가시킴으로써 염 스트레스의 경감 및 생장 촉진에 관여함을 알 수 있었다. A variety of abiotic stresses limit plant growth and crop productivity. Among the abiotic stress, salinity is one of the major harmful stresses to plants. Plant growth-promoting bacterium was isolated from reclaimed land soil of Kyehwa-do and identified as Pseudomonas. Pseudomonas sp. strain G19 produced $7.5{\mu}g/ml$ of indole acetic acid and solubilized 25% of insoluble phosphate after 36 h cultivation. Also, G19 was able to produce a protein that was structurally homologous to 1-aminocyclopropane-1-carboxylate deaminase of Pseudomonas fluorescens KACC10070 playing a role in reduction of ethylene in plant. The strain G19 increased the biomass of Chinese cabbage seedlings grown in the presence of 150 mM NaCl. The results indicated that the strain G19 promoted the growth of Chinese cabbage seedling under salinity stress through microbe-plant interactions.

항진균 세균과 난용성 인산염 가용화 효모의 혼합 배양액을 이용한 고추 병해의 생물학적 방제

이건웅,민병대,박수정,정원화,고은별,이귀재,채종찬,Lee, Gun Woong,Min, Byung-Dae,Park, Sujeong,Jheong, Weonhwa,Go, Eun Byeul,Lee, Kui-Jae,Chae, Jong-Chan 한국미생물학회 2013 미생물학회지 Vol.49 No.4

고추생장 촉진을 위해 병원균에 대한 방제력과 난용성 인산염에 대한 가용화 활성을 보이는 미생물 혼합배양액의 효과를 검증하였다. Saccharomyces sp. L13은 난용성 인산염에 대한 가용화 활성으로 분리되었으며 Bacillus sp. L32는 고추역병과 고추탄저병에 대한 길항력 활성으로 분리되었다. 특히 L32 균주는 대치배양법과 잎을 이용한 실증실험에서 모두 길항능력을 보였으며 시설재배를 이용한 실증실험에서도 병의 발병율을 저감시켰다. 두 균주의 혼합배양은 각 균주들의 생장율에 영향을 주지 않았으며 고추에 대한 혼합배양액 처리는 고추역병과 고추탄저병의 발병율을 저감시키는 동시에 난용성 인산염의 가용화를 통해 고추의 생장율을 증대시키는 효과를 확인할 수 있었다. 본 결과는 두 균주의 혼합배양액이 작물재배를 위한 미생물제제로서 잠재적 효용성이 높다는 것을 제시한다. This study was to investigate beneficial effects of microbial mixture on red pepper which was capable of promoting plant growth by solubilizing insoluble phosphate as well as protecting plants from pathogenic attack. Saccharomyces sp. L13 was isolated for phosphate solubilizing activity on aluminium phosphate, tricalcium phosphate, calcium hydrophosphate, and magnesium hydrophosphate. On the other hand, Bacillus sp. L32 was isolated for antagonistic activity against Phytophthora capsisi and Colletotrichum gloeosporioides, causing Phytophthora blight and Anthracnose disease in pepper, respectively. The strain L32 exhibited antagonistic activities both under dual culture assays and detached leaves assays. The each strain under the condition of mixed cultivation exhibited the same growth rates as one under pure cultivation. In greenhouse study, the mixed culture showed the both effect of plant growth promotion and reduction of disease symptom development against P. capsisi and C. gloeosporioides providing a potential as effective microbial agent for plant husbandry.

국내 하천에 분포하는 ampicillin 내성균의 다양성

고은별,정인영,김혁,석광설,김벼리,유용재,장예진,채종찬,Go, Eun Byeul,Chung, In-Young,Kim, Hyuk,Seok, Kwang-Seol,Kim, Byeori,Yoo, Yong-Jae,Jang, Yejin,Chae, Jong-Chan 한국미생물학회 2015 미생물학회지 Vol.51 No.4

임상과 자연환경에서 발견되는 항생제내성균의 문제는 보건학적 관심의 대상이 되고 있으며 환경 중의 미생물은 항생제 내성유전자 확산의 한 요인으로 판단되고 있다. 본 연구에서는 국내 하천 7지점으로부터 분리한 배양성 ampicillin 내성균의 다양성을 조사하였다. 분리된 498종의 세균들 중에서 Aeromonas와 Acidovorax 속의 내성균 분포가 가장 높았으며, 66%의 분리균들이 조사된 14종의 항생제 중 6종 이상의 항생제에 내성을 나타내었다. 그리고 7종의 항생제에 내성을 보이는 비율이 19.7%로 가장 높았다. 또한 항생제감수성 조사 결과를 바탕으로, 분포율이 높았던 Aeromonas, Acidovorax, Pseudomonas, Elizabethkingia 속의 내성균에 대한 자연내성 특징을 파악하였다. The widespread emergence of antibiotic resistant microorganisms in clinics and natural environments has attracted public concern. Especially, microorganisms inhabiting natural environment is considered as a source responsible for increasing the abundance of antibiotic resistant genes in ecosystem. In this study, the diversity of culturable bacteria resistant to ampicillin was investigated with water samples collected from seven locations in Korea. The genera belonging to Aeromonas and Acidovorax were dominant among the isolated 498 strains. The 66% of isolates showed multi-drug resistance against more than six antibiotics among tested fourteen ones and isolates resistant to seven antibiotics were the most prevalent with 19.7% abundance. Using the antibiotics susceptibility results, the intrinsic resistance profile was suggested for the most dominant genera, Aeromonas, Acidovorax, Pseudomonas, and Elizabethkingia.

하수처리장 방류수에 존재하는 항생제 내성인자가 하천에 미치는 영향

장예진,유용재,설우준,차창준,이옥재,채종찬,Jang, Yejin,Yoo, Yong-Jae,Sul, Woo Jun,Cha, Chang-Jun,Rhee, Ok-Jae,Chae, Jong-Chan 한국미생물학회 2017 미생물학회지 Vol.53 No.4

하수처리장 방류수와 하천 중의 항생제 내성인자 분포에 대한 상관성을 분석하기 위해 방류수와 상류 하천수, 하류 하천수를 대상으로 항생제 내성유전자와 전파 관련 유전자를 조사하였다. 3개 지점에서 134~183개의 항생제 내성유전자(ARG) 및 전파 관련 유전자(MGE)가 검출되었으며, 1개의 16S rRNA 유전자에 대한 ARG 및 MGE 유전자의 상대적인 총 합이 0.063~0.422 copy로 분석되었다. ARG와 MGE의 수와 존재량은 방류수에서 가장 높게 검출된 반면, 총 세균 수는 가장 적게 검출됨으로서 하수처리 과정에서 사멸된 세균에 포함된 유전자들이 검출된 것으로 판단된다. 또한 MGE의 존재량 양상이 ARG의 존재량과 상관관계를 보임으로서 항생제 내성균들의 내성기작이 자연내성보다는 획득내성일 가능성을 제시하였다. The antibiotic resistant genes (ARG) and mobile genetic elements (MGE) were investigated with the effluent of waste-water treatment plant (WWTP), and river waters of upstream and downstream in order to elucidate the effect of effluent on antibiotic resistance in a natural river. Total numbers of 134~183 of ARG and MGE were detected and the abundance of ARG and MGE was 0.063~0.422 copies per one of 16S rRNA gene in three water samples. Effluent sample contained the highest amount of the total number and abundance of ARG and MGE whereas total viable cells were observed in the lowest amount among the three samples. This indicated that the genes were originated from cells died during the wastewater treatment process. In addition, the co-relationship of abundance between ARG and MGE suggested that acquired resistance was a prevalent mechanism among the antibiotic-resistant bacteria existing in WWTP.

지상발표 : Achalasia 의 3예

전우규(Woo Kue Jun),정인교(In Kyo Chung),채종찬(Jong Chan Chae),김향(Hyang Kim),박영철(Young Cheol Park),이사종(Sa Jong Lee) 대한소화기학회 1987 대한소화기학회 추계학술대회 Vol.1987 No.-

지상발표 : 위장관 악성종양에 있어서 몇가지 종양 표식자의 임상적 의의

서영주(Young Joo Suh),김철우(Cheol Woo Kim),전우규(Woo Kue Jun),채종찬(Jong Chan Chae),이승세(Seung Se Lee),정을순(Eul Soon Chung),이상종(Sang Jong Lee) 대한소화기학회 1987 대한소화기학회 추계학술대회 Vol.1987 No.-

초등학생의 당, 나트륨, 지방 섭취 저감화를 위한 교육용 웹사이트 및 e-Learning system 개발

김형우(Hyung Woo Kim),이경애(Kyoung Ae Lee),조은진(Eun Jin Cho),채종찬(Jong Chan Chae),김유경(Yoo Kyeong Kim),이성숙(Sung Sug Lee),이경혜(Kyung Hea Lee) 대한지역사회영양학회 2010 대한지역사회영양학회지 Vol.15 No.1

This study was carried out to reduce children`s sugar, natrium and fat intakes and establish their healthy lifestyle. To achieve these goals, we developed an educational web site and an e-learning system. The targets of this web site are children, parents and educators. This site has various information about sugar, natrium and fat, and has special menus for each target: such as the `Let`s study` for children, `Guides for child`s eating` for parents, and `Educational softwares, Lesson plans/materials, and Textbooks/Teaching guides` for educators. We developed three nutrient characters and applied them to the web site. We provided information in the form of texts, images, flash and sounds. This site has special boards in `Nutrition cafe` menu to interchange information or their successful stories between the connecters. We developed an e-learning system with two courses. One is for junior elementary students and the other is for senior students. Children can study each nutrient step by step in a course according to their academic ability and concern. Also, they can evaluate their academic achievement in this system, which was uploaded into `Let`s study` in children menu in the web site. Conclusively this web site and e-learning system could contribute to reducing children`s sugar, natrium and fat intakes by helping children study them systematically and effectively by on-line system. We expect this e-learning system would be a new nutrition education system to make nutrition education more active. (Korean J Community Nutrition 15(1): 36~49, 2010)