인체적용시험을 통한 한식 우수성 구명연구

채수완(Soo-Wan Chae) 한국식품영양과학회 2011 식품산업과 영양 Vol.16 No.2

의료ㆍ농업 융복합시스템의 방향과 미래예측

채수완(Soo-Wan Chae) 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

에그로메디컬 융복합 R&D사업의 분석체계 구축에 관한 연구

채수완 ( Chae Soo Wan ),소병옥 ( Byung Ok So ),주병철 ( Byeoung Chul Ju ) 대한관광경영학회 2014 觀光硏究 Vol.29 No.5

본 연구의 목적은 에그로메디컬 융복합 R&D사업의 분석체계를 개발하고 내ㆍ외부의 전문가를 대상으로 분석지표별 중요도를 분석하는 것이다. 이를 위해 분석지표에 대한 국내외 선행연구를 바탕으로 세 가지 관점을 도출하고 각 관점별로 세부적 분석항목과 지표를 추출하였으며, 관련 전문가들을 대상으로 의견조사를 실시하여 적합성 여부를 검토한 후 최종 분석체계를 구축하였다. 구축된 분석체계는 정책적타당성, 기술적타당성, 경제적타당성의 세 가지 영역으로 분류되고 핵심 분석항목은 8가지 항목으로, 세부 분석지표는 29가지로 구성하였다. 구축된 분석체계의 중요도 및 우선순위의 분석을 위해 학계와 기업 그리고 관련 기관 등의 전문가를 대상으로 설문조사를 시행하였으며, 분석방법으로 AHP분석을 사용하였다. 구축된 분석체계의 세 가지 관점별 중요도를 분석한 결과, 전문가들은 기술적타당성, 경제적타당성, 정책적타당성 순으로 상대적 중요도를 높게 판단하였다. The purpose of this study was to develop an analysis system for an agro-medical convergence R&D project and to assess the importance of each analysis indicator through internal and external interviews with experts. To this end, the study identified three perspectives from domestic and overseas studies previously conducted on analysis indicators, extracted detailed items and analysis indicators for each perspective, reviewed goodness of fit by conducting opinion research among relevant experts, and established a final analysis system. The final system is classified into three areas: policy validity, technical validity, and economic validity. There were eight key analysis items and 29 detailed analysis indicators. To assess their importance and order of priority for the analysis system, a survey was conducted among experts in academia, industry, and other relevant organizations. The analytic hierarchy process (AHP) was used for this. The results, assessing the importance of the three perspectives, revealed that the experts believed technical validity to have the highest relative importance, followed by economic validity, and then policy validity.

M&A 및 전략적 제휴를 통한 UCC 기반 서비스 확보 전략 분석: 망외부성 이론 관점에서의 성공 및 실패 사례 분석

채수완 ( Soo Wan Chae ),유병준 ( Byung Joon Yoo ) ㈜엘지씨엔에스 2007 Entrue Journal of Information Technology Vol.6 No.1

최근, 웹 2.0이 인터넷 비즈니스에서 화두가 되고 있다. IT 기업들은 변화하는 웹 환경에 적응하기 위해 기술적 해결책을 모색하고 있으며, 서비스의 변화를 추구하고 있다. 웹 2.0을 대표하는 핵심 가치인 참여, 공유, 개방을 담고 있는 UCC(User Created Contents) 기반 서비스가 그 변화의 핵심 서비스로 주목 받게 됨에 따라 IT 기업들은 UCC 기반 서비스 및 컨텐츠 확보에 열을 올리고 있다. 본 연구는 IT 기업들의 효과적인 UCC 서비스 및 컨텐츠 확보 전략에 있어서 M&A 및 전략적 제휴를 매우 효과적인 대안으로 제시하고 분석하고자 한다. 이를 위하여, 본 논문은 국내외 주요 IT 기업들의 다양한 사례들을 바탕으로 분석함으로써 M&A 및 전략적 제휴를 통한 UCC 서비스 확보 방안의 효율성을 분석하였다. 이 분석에 있어 본 논문은 망외부성 효과의 성패 여부를 설명함에 있어 중요한 이론적 배경으로 제시하고자 한다. 본 연구는 경제이론을 바탕으로 한 분석의 틀을 제시함으로써 UCC 기반 서비스 확보에 있어서의 실무적 시사점과 관리적 통찰력을 IT 기업들에게 제공해 줄 수 있을 것으로 기대된다. Recently, Web 2.0 has been a red hot topic in e-Business. IT companies are trying to find technological solutions to change their services in order to quickly respond to the rapidly changing web environment. In particular, IT companies are desperately seeking ways to acquire UCC based services that are now at the core of Web 2.0 which characteristics include participation, distribution, and open distinguished from the current web environment of Web 1.0. In this study, we discuss M&A and strategic alliances as two effective ways to acquire UCC based services and their contents. We qualitatively analyze several cases of M&A and strategic alliances that major Korean and foreign IT companies have experienced during UCC acquisition. We suggest network externalities theory as a theoretical background to explain the success and failure of M&A and strategic alliances. From this study, we expect to provide practical implications and managerial insights to IT companies on M&A and strategic alliances for UCC acquisition supported by an economic theory.

Clinical Trials for Respiratory Health of Functional Foods

Soo-Wan Chae(채수완) 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

개구리 피부(皮腐)의 막전위(膜電位) 및 단락전류(短絡電流)에 미치는 Morphine의 영향

채수완,조규박,Chae Soo-Wan,Cho Kyu-Park 대한약리학회 1984 대한약리학잡지 Vol.20 No.1

정상 또는 morphine을 처리한 개구리(Rana nigromaculate)의 피부에서 Ussing 등의 방법에 의하여 막전위와 단락전류에 미치는 morphine의 작용과 morphine 작용에 미치는 전해질의 영향을 관찰하여 다음과 같은 성적을 얻었다. 1) Morphine $5{\pm}10^{-3}$은 개구리 피부의 막전위와 단락전류를 현저히 감소시켰으며 약물투여후 60분후에 최고의 효과를 나타냈다. 2) Naloxone $10^{-4}$은 morphine의 막전위 및 단란전류 억제작용을 차단치 못하였다. 3) 영양액내 $Na^+$ 농도감소, $K^+$ 농도증가 및 $Ca^{2+}$농도증가는 morphine의 막전위 및 단란전류 억제작용ㅇ르 현저히 강화하였으며, $Mn^{2+}$첨가는 morphine의 막전위 억제효과만을 강화시켰다. 한편 $Ca^{2+}$ 농도의 감소로써 morphine의 단락전류억제 효과는 현저히 약화되었으며 $Ma^{2+}$농도 감소로써 morphine의 단락전류 억제효과로 더불어 막전위 억제효과도 감약되었다. 4) Morphine 처리 표본에서 막전위와 단락전류는 morphine 처리 초기에 일시적인 감소를 일으킨 후 증가하여 morphine 처리 $4{\sim}8$일에는 대조군에 비하여 현저히 상승하였다. 또한 morphine의 막전위 및 단락전류 억제효과에 있어서도 morphine처리 $1{\sim}2$일에는 morphine의 억제효과가 강화되나 morphine의 처리 4 또는 8일에는 현저히 약화되었다. 이상의 실험성적으로 morphine의 약물내성을 포함한 그 약리적작용이 생체막에서의 전해질수송 또는 전해질 투과성에 영향을 미쳐 초래될 수 있음을 시사하는 것으로 추측하였다. The effects of morphine on the transmembrane potential and the short circuit current in the isolated frog skin were studied under different experimental conditions. The measurem ents of the transmembrane potential and the short circuit current were carried out according to Ussing and Zerahn's method. Experimental results were summerized as follows: 1) $5{\pm}10^{-3}$M of morphine markedly depressed the transmembrane potential and the short circuit current of the naive preparation. The peak of these inhibitory effects of morphine was observed about 1 hour after administration of the drug. 2) However $10^{-4}$M of naloxone did not affect these effects of morphine. 3) Decrease of $K^+$, increase of $K^+$ or $Ca^{2+}$ in the perfusate, markedly potentiated the inhibitory action of morphine on both transmembrane potential and short circuit current of the frog skin, and addition of $Mn^{2+}$ to the solution depressed the effect of morphine on the transmembrane potential, while the inhibitory effect of morphine on the short circuit current was diminished in the $Ca^{2+}$-free ringer solution, and increase of $Mg^{2+}$ concentration depressed those effect of morphine on both electrical parameters. 4) In the morphine treated preparations, transmembrane potential and short circuit current were decreased in the early phase of drug treatment ($1{\sim}2$ days), but gradually increased to the significantly high level from the control (48 days after treatment). In these preparations, the effects of morphine on both electrical parameters were also potentiated in the early phase, but markedly diminished in the late phase of treatment. From the above results, it is postulated that the pharmacological actions of morphine as well as development of the tolerance by morphine may be partially related to the changes of ion fluxes and/or permeabilities of skin by the drug.

백서(白鼠) 뇌조직(腦組織)에서 Morphine의 Saturable Binding에 미치는 전해질(電解質)의 영향(影響)

고복현,채수완,조규박,Ko, Bok-Hyun,Chae, Soo-Wan,Cho, Kyu-Park 대한약리학회 1982 대한약리학잡지 Vol.18 No.2

전해질농도(電解質濃度)를 달리하고 morphine, naloxone 또는 morphine+naloxone을 가(加)하거나 가(加)하지 않은 modified Krebs-Henseleit bicarbonate buffer용액(溶液)에 흰쥐의 뇌절편(腦切片)을 incubate하여 저온처리(低溫處理)와 media내(內) 전해질조성(電解質組成)이 $(^3H)-morphine$의 saturable binding에 미치는 영향(影響)을 관찰(觀察)하여 다음과 같은 성적(成績)을 얻었다. 1) 저온처리(低溫處理)는 $(^3H)-morphine$의 saturable binding을 현저(顯著)히 증가(增加)시켰고, maximal saturable binding은 증가(增加)시키나 $K_D$치(値)에는 영향(影響)을 미치지 못하였다. 2) 저온처리시(低溫處理時) media내 morphine과 naloxone은 $(^3H)-morphine$ 의 maximal saturable binding 과 $K_D$치(値)를 증가(增加) 시켰다. 3) 저온처리시(低溫處理時) media내(內) $K^+$감소(減少), $Mg^{++}$제거 또는$Mn^{++}$첨가(添加)는 $(^3H)-morphine$의 saturable binding을 현저(顯著)히 증가(增加)시켰고, $Na^+$감소(減少), $Ca^{++}$증가(增加)는 saturable binding에 영향(影響)을 미치지 못하였다. 4) 저온처리시(低溫處理時) media내(內) morphine에 의한 $(^3H)-morphine$의 saturable binding증가(增加)는 media내(內) $Na^+$감소(減少), $K^+$감소(減少), $Mg^{++}$제거 또는 $Mn^{++}$첨가(添加)로는 영향(影響) 받지 않았으나 $Ca^{++}$증가(增加)로 억제(抑制)되었고, media내(內) naloxone에 의한 saturable binding증가(增加)는 $Mn^{++}$첨가(添加)는 영향(影響)받지 않았으나 media내(內) $Na^+$감소(減少), $K^+$감소(減少), $Ca^{++}$증가(增加) 또는 $Mg^{++}$제거로 억제(抑制)되었다. 이상(以上) 실험성적(實驗成績)은 저온처리(低溫處理) 및 저온처리시(低溫處理時) media내(內) morphine 또는 naloxone이 opiate수용체(受容體)의 양적(量的)인 변동(變動)과 또는 친화력(親和力)의 변동(變動)을 유발(誘發)하며, media내(內) 전해질조성(電解質組成)이 이들 변동(變動)에 영향(影響)을 미침을 시사(示唆)한다. The binding in vitro of an opiate agonist, $(^3H)-morphine$, was studied using rat brain slices which were incubated in the modified Krebs-Henseleit bicarbonate buffer solution containing various concentrations of electrolytes with or without morphine, naloxone or morphine+naloxone at $4^{\circ}C$ for 24 hours. The binding of $(^3H)-morphine$ may be seperated into two component; one a saturable binding and the other nonsaturable. The saturable binding may be calculated from the differences in binding observed in the absence and presence of high concentration of morphine. The maximal saturable binding and $K_D$ value in the naive preparations were $0.32{\pm}0.02\;pmole/mg$ protein and $0.75{\pm}0.07\;nM$ respectively. The saturable binding of $(^3H)-morphine$ was significantly increased by low temperature-treatment, while $K_D$ value was not changed. Morphine in the incubation media significantly increased the saturable binding of $(^3H)-morphine$ and $K_D$ value. Naloxone also increased the maximal saturable binding of $(^3H)-morphine$ and $K_D$ value of the drug. Decrease of $K^+\;and\;Mg^{++}$, and addition of $Mn^{++}$ in the incubation media significantly increased the saturable binding of $(^3H)-morphine$, but decrease of $Na^+$and increase of $Ca^{++}$ in the incubation media did not influence the binding. The increment of the saturable binding of $(^3H)-morphine$ by nonlabeled morphine in the incubation media was notaffected by decrease of $Na^+,\;K^+\;or\;Mg^{++}$, or addition of $Mn^{++}$ into the incubation media, but was inhibited by increase of $Ca^{++}$ in the incubation media, while the increment of the saturable binding of $(^3H)-morphine$ was net observed by decrease of $Na^+,\;K^+\;or\;Mg^{++}$, or increase of $Ca^{++}$ in the incubation media. The above results indicate that change of opiate binding sites in quality, i.e. affinity, and quantity, i.e. number of binding sites, may occur by low temperature-treatment in the absence and presence of morphine or naloxone and that electrolytes play role of the changes of opiate binding sites.

Effects of ${\alpha}_1-Adrenergic$ Stimulation on Membrane Potential, Twitch Force, Intracellular $Na^+,\;and\;H^+$ Activity in Hyperthyroid Guinea Pig Ventricular Muscle

김진상,채수완,조규박,Kim Jin-Sang,Chae Soo-Wan,Cho Kyu-Park The Korean Society of Pharmacology 1995 대한약리학잡지 Vol.31 No.1

갑상선 기능 항진증 심장에서의 ${\beta}-adrenoceptor$의 역할은 잘 알려져 있으나 ${\alpha}-adrenoceptor$에 대해서는 알려져 있지 않은 바, 저자 등은 갑상선 기능 항진증 기니픽 심장의 유두근에서 일반 미세 전극과 이온-선택적 미세 전극을 이용하여 세포내 $Na^+$과 $H^+$ 활성도에 대한 phenylephrine의 영향을 연구하였다. Phenylephrine ($10^{-5}$ 또는 $3{\times}10^{-5}M$)에 의한 ${\alpha}_1-adrenoceptor$ 자극은 다양한 활동전위의 변동, 수축기 막전위의 과분극 ($1.5{\pm}0.1mM$), 세포내 활성도 증가 ($0.4{\pm}0.15mM$), 현저한 수축력 증가 ($220{\pm}15%$) 그리고 세포내 pH의 증가 ($0.06{\pm}0.002\;unit$)를 일으켰고, 이와 같은 변동이 prazosin과 atenolol에 의해 차단되었다. 그래서 이들 효과가 ${\alpha}_1-adrenoceptor$를 경유함을 알 수 있었고, 역시, 세포내 $Na^+$ 활성도와 수축력 증가 효과가 $Na^{+}-H^{+}$ 교환기 억제제인 ethylisopropylamiloride로 차단됨으로 보아 ${\alpha}_1-adrenoceptor$ 자극은 $Na^{+}-H^{+}$ 교환기를 자극하여 세포내 $a^{i}_{Na}$와 pH를 증가시킴을 시사한다. 이는 ${\alpha}_1-adrenoceptor$ 자극에 의한 세포내 $Na^+$감소와 초기 수축력 감소 효과를 나타내는 정상 기니픽 심장과는 매우 다른 결과로 갑상선 기능 항진증 심장에서 ${\alpha}_1-adrenoceptor$는 매우 중요한 기능을 갖고 있음을 의미한다. The roles of ${\beta}-adrenoceptor$ were well known in hyperthyroidal heart, but not with ${\alpha}-adrenoceptor$. So we studied the effects of phenylephrine on membrane potential, intracellular sodium activity ($a^{i}_{Na}$), twitch force, and intracellular pH ($pH_i$) by continuous intracellular recordings with ion-selective and conventional microelectrodes in the papillary muscles of hyperthyroid guinea pig heart. ${\alpha}_1-adrenoceptor$ stimulation by phenylephrine (10^{-5}\;or\;3{\times}10^{-5}M$) produced the following changes: variable changes in action potential duration, a hyperpolarization ($1.5{\pm}0.1mM$) of the diastolic membrane potential, an increase in $a^{i}_{Na}\;(0.4{\pm}0.15mM)$, a stronger positive inotropic effect ($220{\pm}15%$), an increase in $pH_i\;(0.06{\pm}0.002\;unit)$. These changes were flocked by prazosin and atenolol. This indicated that the changes in membrane potential, $a^{i}_{Na}$ twitch force, and $pH_i$ are mediated by a stimulation of the ${\alpha}_1-adrenoceptor$. Ethylisopropylamiloride ($10^{-5}$) also blocked the increase in $a^{i}_{Na}$ and twitch force. On the other hand, strophanthidin, tetrodotoxin, $Cs^+$ or verapamil did not block the increase in $a^{i}_{Na}$ and twitch force. Thus, it was suggested that ${\alpha}_1-adrenoceptor$ stimulation increased $a^{i}_{Na}\;and\;pH_i$ by stimulation of $Na^{+}-H^{+}$ exchange, thereby allowing intracellular alkalinization and $a^{i}_{Na}$ increase. These results were very different from euthyroidal heart which showed ${\alpha}_1-adrenoceptor$-induced decrease in $a^{i}_{Na}$ and initial negative inotropic effect. From the above results, it was concluded that ${\alpha}_1-adrenoceptor$ had a important role in hyperthy-roidal heart.

사람심방 K+ 통로 활성에 대한 H2O2의 효과

이완구 ( Wan Goo Lee ),김태균 ( Tae Kyun Kim ),채수완 ( Soo Wan Chae ) 전북대학교 의과학연구소 2001 全北醫大論文集 Vol.25 No.2

Hydrogen peroxide의 농도에 따른 심장기능 조절작용을 규명하고자, 심방에서 중요한 기능을 한다고 알려진 hKv1.5유전자를 HEK-293 cell 및 Ltk-cell에 발현시켜 유도된 K+전류 및 사람 심방세포의 K+전류에 미치는 hydrogen peroxide의 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. Hydrogen peroxide는 HEK-293 cell 및 Ltk-cell에서 Kv1.5 전류의 activation curve의 전압의존성을 과분극쪽으로 약 20mV정도 이동시켜 전류를 증가시켰다. 또한, HEK-293 cell에서 Kv1.5/Kvb1.3전류의 activation curve의 전압의존성을 이동시켰을뿐만 아니라 fast inactivation을 하였다. 2. Ltk-cell에서 0.01-0.3 mM 이상에서는 현저히 감소시켰다. 3. 사람 심방세포에서 1 mM의 hydrogen peroxide는 steady state outward 전류를 5배이상 증가시켰다. 이상의 실험성적은 hydrogen peroxide가 농도에 따라 hKv1.5전류를 조절할수 있음을 밝혔는데, 특히 비교적 낮은 농도의 hydrogen peroxide는 이 전류를 증가시킴을 사람심방세포에서 확인하였다. During myocardial ischemia and subsequent reperfusion, reactive oxygen species are an important mediator of cellular damage and rhythm disturbances. H2O2, a small, uncharged and relatively stable molecule that diffuses easily through tissue, has several adverse effects on the myocardium including induction of cardiac arrhythmias. Although several previous investigations have described electrophysiological effects of H2O2 in mammalian myocardium, the underlying ionic mechanisms of H2O2 effects remain unclear. In addition, voltage-gated K` channels represent the most complex group of ion channel genes expressed in cardiovascular system. The human Kv1.5 channel (hKv1.5) represents the IKur repolarizing current in atrial myocytes. The hKv1.5 channel is functionally modulated by the Kvb1.3 subunit, which converts it from a delayed rectifier to a channel with rapid inactivation and enhanced voltage sensitivity. In present study, we examined the effects of H2O2 following coexpression of hKv1.5 with the Kvb1.3 subunit in Ltk-cells and HEK-293 cells. H2O2 (1 mM) reversibly increased the K` currents in the cells transected with hKv1.5 and/or Kvb1.3 or Kvb2.1 subunits. Additionally, H2O2 Slowed deactivation and fast inactivation in the cells cotransfected with hKv1.5 and/or Kvb1.3 or Kvb2.1, and made the hyperpolizing shift of activation curve by about 20 mV. However higher concentration of H2O2 (higher than 1mM) decreased the hKv1.5/Kvb2.1 current. Catalase (1000ц/ml) itself accelerated the inactivation process of hKv1.5 current and decreased the steady-state current. In isolated human atrial myocytes. H2O2 (1mM) reversibly increased the outward K` currents by about five times. These results reported that H2O2 has a dual effect on hKv1.5 current, thereby being able to explain the potential roles of H2O2 during myocardial reperfusion injury.

기니픽 심근의 수축력과 세포내 $Na^+$ 활성도에 미치는 ${\alpha}_1-Adrenergic$ 수용체 자극효과

김진상,강형섭,채수완,이진옥,Kim, Jin-Sang,Kang, Hyung-Sub,Chae, Soo-Wan,Lee, Chin-Ok 대한약리학회 1996 대한약리학잡지 Vol.32 No.2

Myocardial ${\alpha}_1-adrenoceptors$ have been shown to mediate a biphaslc inotropic response that was characterized by a transient decline followed by a sustained increasing phase in guinea pig ventricular muscle. Recently one group reported that an ${\alpha}_1-adrenoceptors-induced$ intracellular $Na^+$ decrease is linked to fast $Na^+$ channel inhibition and another group reported that it is linked to $Na^+$-$K^+$ pump activation by ${\alpha}_{1b}-adrenoceptors$. But until now, its mechanism is not clear. Therefore, to see whether the $Na^+$channel or $Na^+-K^+$ pump is related to a decrease in intracellular $Na^+$ activity and/or the negative inotropic response, and which ${\alpha}_1-adrenoceptor$ subtype was involved in the decrease in intracellular $Na^+$activity by phenylephrine, we used conventional and sodium selective microelectrodes, and tension transducer to determine the effects of ${\alpha}_1-adrenergic$ stimulation on membrane potential, intracellular $Na^+$ activity, and twitch force in guinea pig ventricular muscles. $10^{-5}$ M Phenylephrine produced a slight hyperpolarization of the diastolic membrane potential, a decrease or increase in $a_N^i_a$, and a biphasic inotropic response. The negative inotropic response accompanied by a decrease in intracellular $Na^+$activity, whereas in muscles showing a remarkable positive inotropic response without initial negative inotropic effect was accompanied by an increase in intracellular $Na^+$ activity. The decrease in intracellular $Na^+$ activity was apparently inhibited by WB4101, an antagonist of the ${\alpha}_{1a}-adrenoceptors$. The decrease in intracellular $Na^+$ activity caused by phenylephrine was not abolished or reduced by a block of the fast $Na^+$ channels. $V_{max}$ also was not affected by phenylephrine. Phenylephrine produced an increase in intracellular $Na^+$ activity in the presence of a high concentration of extracellular $Ca^{2+}$ (in quiescent muscle) or phorbol dibutyrate, a protein kinase C activator(in beating muscle). These suggest that the ${\alpha}_{1a}-adrenoceptors-mediated$ decrease in intracellular $Na^+$ activity may be related to the protein kinase C.